Prestained SDS-PAGE Standards

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Overview

Kaleidoscope Standards

Kaleidoscope prestained standards are broad range and have individually colored proteins to allow instant orientation on SDS-PAGE gels and western blots.


Kaleidoscope prestained standards
Calibrated Molecular Weights of Kaleidoscope Prestained Standards.
Protein Color MW (kD)
Myosin Blue 216.0
β-Galactosidase Magenta 132.0
BSA Green 78.0
Carbonic anhydrase Violet 45.7
Soybean trypsin inhibitor Orange 32.5
Lysozyme Red 18.4
Aprotinin Blue 7.6


MWs are of representative lots; actual weights may vary. Lot-specific MWs are included with each vial.

Prestained SDS-PAGE Standards
Prestained SDS-PAGE Standards


Use prestained SDS-PAGE standards to assess the efficiency of electrophoretic transfer on western blots. Broad range prestained SDS-PAGE standards, 5 µl, were run on a 4–20% Ready Gel® precast gel and transferred to nitrocellulose in the Mini Trans-Blot® cell. Bio-Rad's original prestained SDS-PAGE standards are available in high, low and broad ranges.


Calibrated Molecular Weights of Prestained SDS-PAGE Standards.
  Ranges Available
Protein MW (kD) Broad Low High
Myosin 210.0  
β-Galactosidase 125.0  
Phosphorylase b 113.0    
Bovine serum albumin (BSA) 101.0
Ovalbumin 56.2
Carbonic anhydrase 35.8  
Soybean trypsin inhibitor 29.0  
Lysozyme 21.0  
Aprotinin 6.9    


MWs are of representative lots; actual weights may vary. Lot-specific MWs areincluded with each vial.

Prestained standards for SDS-PAGE and western blotting provide a quick and easy way to monitor protein separation during electrophoresis and to assess transfer efficiency on blots. Each lot of Kaleidoscope and SDS-PAGE prestained standards is individually calibrated for estimating the MW of sample proteins.

Kaleidoscope™ Standards

Kaleidoscope prestained standards are broad range and have individually colored proteins to allow instant orientation on SDS-PAGE gels and western blots.

  Calibrated Molecular Weights* of Kaleidoscope Standards

Protein Color Prestained
Myosin Blue 216,000
β-Galactosidase Magenta 132,000
BSA Green 78,000
Carbonic anhydrase Violet 45,700
Soybean trypsin inhibitor Orange 32,500
Lysozyme Red 18,400
Aprotinin Blue 7,600

* MWs are of representative lots; actual weights may vary. Lot-specific MWs are included with each vial.

Prestained SDS-PAGE Standards

Original Bio-Rad prestained SDS-PAGE standards are available in broad, low, and high ranges.

  Calibrated Molecular Weights* of Prestained SDS-PAGE Standards

Protein Broad Range Low Range High Range
Myosin 209,000 204,000
β-Galactosidase 124,000 123,000
Phosphorylast b 103,000
BSA 80,000 77,000 80,000
Ovalbumin 49,100 50,000 48,000
Carbonic anhydrase 34,800 34,300
Soybean trypsin inhibitor 28,900 28,800
Lysozyme 20,600 20,700
Aprotinin 7,100

* MWs are of representative lots; actual weights may vary. Lot-specific MWs are included with each vial.

MW size range Varies by standard
Stain type Stained
Volume 500 µl
Shelf life 12 months
Kaleidoscope Prestained Standards

161-0324
500 μl, broad-range standards (MW 7,600–216,000), seven individually colored proteins

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Prestained SDS-PAGE Standards

161-0305
500 μl, low range standards (20,700–103,000 MW), prestained mixture of six proteins

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Prestained SDS-PAGE Standards

161-0309
500 μl, high range standards (48,000–204,000 MW), prestained mixture of four proteins

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Prestained SDS-PAGE Standards

161-0318
500 μl, broad range standards (7,100–209,000 MW), prestained mixture of eight proteins

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Number Description Options
2998 Protein Standards Application Guide, Rev G Click to download [ Add to Cart (Free) ]
3118 Protein Standards for Electrophoresis and Blotting Brochure, Rev D Click to download [ Add to Cart (Free) ]
2414 The Little Book of Standards, Rev D Click to download [ Add to Cart (Free) ]
2032 Western Blotting Detection Reagents Brochure, Rev F Click to download [ Add to Cart (Free) ]
5561 Precision Plus Protein WesternC Standards, Rev E Click to download [ Add to Cart (Free) ]
6148 Transfer of High Molecular Weight Proteins to Membranes: A Comparison of Transfer Efficiency Between Blotting Systems, Rev B Click to download [ Add to Cart (Free) ]
4006026 Instruction Manual, Prestained SDS-PAGE Standards, Broad Range, Rev D Click to download
4006024 Instruction Manual, Prestained SDS-PAGE Standards, Low Range, Rev D Click to download
4006025 Instruction Manual, Prestained SDS-PAGE Standards, High Range, Rev B Click to download
LIT599 Instruction Manual, Kaleidoscope Prestained Standards, Rev E Click to download
4006029 Instruction Manual, Kaleidoscope Polypeptide Standard, Rev B Click to download
2000 Polypeptide Standards: SDS-PAGE and Kaleidoscope Standards PIS, Rev B Click to download