Unstained SDS-PAGE Standards

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Overview

Specifications
Natural Unstained Standards Specifications

  Volume Applications*
(Number of Mini Gels)

SDS-PAGE standards 200 µl 800–1,600
Polypeptide standards 200 µl 800

* Number of applications depends on staining method.

Discontinued Products for Unstained SDS-PAGE Standards
Discontinued Silver Stain SDS-PAGE Standards   
Recommended Options for Replacement     
161-0314 Low Range 161-0363  
161-0304  
161-0317  
Precision Plus Protein™ Unstained
SDS-PAGE Unstained Low Range
SDS-PAGE Unstained Broad Range
161-0315 High Range
161-0363  
161-0303  
161-0317  
Precision Plus Protein™ Unstained
SDS-PAGE Unstained High Range
SDS-PAGE Unstained Broad Range

Unstained SDS-PAGE Standards

Unstained natural SDS-PAGE standards are available in broad, low, high, and polypeptide MW ranges, allowing calibration in almost any percentage gel.


Natural unstained standards


Natural unstained standards.


Constituent proteins of natural unstained SDS-PAGE standards.
  Ranges Available*
Protein Source MW (kD) Broad Low High Polypeptide
Myosin Rabbit skeletal muscle 200.0    
β-Galactosidase E. coli 116.3    
Phosphorylase b Rabbit muscle 97.4  
Serum albumin Bovine 66.2  
Ovalbumin Hen egg white 45.0
 
Carbonic anhydrase Bovine 31.0    
Triosephosphate isomerase Rabbit 26.6      
Trypsin inhibitor Soybean 21.5    
Myoglobin Equine 17.0      
a-Lactalbumin Bovine 14.5      
Lysozyme Hen egg white 14.4    
Aprotinin Bovine pancreas 6.5    
Insulin B chain, oxidized Bovine 3.5      
Bacitracin Bacillus licheniformis 1.4        •


* SDS-PAGE — high, low, broad, and polypeptide.

Unstained standards allow accurate MW determination on SDS-PAGE gels. Every batch is tested for proper mobility, providing a reliable control for gel-to-gel variability.

SDS-PAGE Standards

SDS-PAGE standards are blended to give uniform band intensities when stained with Coomassie Blue R-250 or zinc stains. SDS-PAGE standards are available in broad, low, high, and polypeptide molecular weight ranges, allowing calibration in almost any percentage gel.

Polypeptide SDS-PAGE standards are for molecular weight determination of peptides and small proteins resolved on Tricine gels. Consisting of six polypeptides with molecular masses ranging from ~1.4 to ~26.6 kD, polypeptide SDS-PAGE standards stain uniformly with Coomassie Blue G-250 stain.

 

SDS-PAGE Standards

161-0303
200 μl, high range standards (45,000–200,000 MW), unstained mixture of five proteins

List Price:   $105.00
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SDS-PAGE Standards

161-0304
200 μl, low range standards (14,400–97,400 MW), unstained mixture of six proteins

List Price:   $92.00
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SDS-PAGE Standards

161-0317
200 μl, broad range standards (6,500–200,000 MW), unstained mixture of nine proteins

List Price:   $110.00
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Polypeptide SDS-PAGE Standards

161-0326
200 μl, ~1,400–~26,600 MW range, unstained mixture of six polypeptides

List Price:   $85.00
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Number Description Options
2032 Western Blotting Detection Reagents Brochure, Rev F Click to download [ Add to Cart (Free) ]
2000 Polypeptide Standards: SDS-PAGE and Kaleidoscope Standards PIS, Rev B Click to download
2414 The Little Book of Standards, Rev D Click to download [ Add to Cart (Free) ]
2998 Protein Standards Application Guide, Rev F Click to download [ Add to Cart (Free) ]
3118 Protein Standards for Electrophoresis and Blotting Brochure, Rev C Click to download [ Add to Cart (Free) ]
5561 Precision Plus Protein WesternC Standards, Rev E Click to download [ Add to Cart (Free) ]
4006047 Instruction Manual, Silver Stain SDS-PAGE Standards, Low Range, Rev C Click to download
4006048 Instruction Manual, Silver Stain SDS-PAGE Standards, High Range, Rev D Click to download
4006046 Instruction Manual, Polypeptide SDS-PAGE Molecular Weight Standards, Rev B Click to download
4006035 Instruction Manual, SDS-PAGE Molecular Weight Standards, Broad Range, Rev C Click to download
4006034 Instruction Manual, SDS-PAGE Molecular Weight Standards, High Range, Rev C Click to download
LIT442 Instruction Manual, Silver Stain Plus, Rev E Click to download
9532 Instruction Manual, SDS-PAGE Molecular Weight Standards, Low Range, Rev C Click to download
6148 Transfer of High Molecular Weight Proteins to Membranes: A Comparison of Transfer Efficiency Between Blotting Systems, Rev B Click to download [ Add to Cart (Free) ]