The Precision Plus Protein™ WesternC™ pack contains five sets of Precision Plus Protein WesternC standards (#161-0376) and Precision Plus Protein™ StrepTactin-HRP conjugate (#161-0381). Precision Plus Protein WesternC standards are a combination standard for western blotting or fluorescence detection. They contain ten Strep-tagged recombinant proteins (10–250 kD), including three pink reference bands (25, 50, and 75 kD), as well as unstained versions of the Strep-tagged recombinant proteins for molecular weight sizing after blot development. StrepTactin-HRP conjugate is needed for colorimetric or chemiluminescent blot detection of the WesternC standard; StrepTactin-AP conjugate (#161-0382) can also be used.
Applications and Uses
Choose Precision Plus Protein WesternC standards for SDS-PAGE and blotting:
- Monitoring electrophoretic separation
- Monitoring blot transfer efficiency with easy orientation via multicolor standards
- Molecular weight sizing of proteins after blot development
- Fluorescent blot detection with prestained bands, with blue bands visualized in the red wavelength range and pink bands in the green wavelength range (prepare a 1:10 dilution of the WesternC standard and load 5 µl per well. No conjugate is needed for fluorescent blot detection)
30% (w/v) glycerol, 2% SDS, 62.5 mM Tris, pH 6.8, 50 mM DTT, 5 mM EDTA, 0.02% NaN3, 0.01% bromophenol blue