Description
Description
Hard-Shell 96-Well 480 PCR Plates are optimized to work on the Roche LightCycler 480 real-time PCR system. Hard-Shell Plates are specifically designed to withstand the stresses of heat sealing, thermal cycling, and robotic handling. The patented* two-component microplate design provides superior stability and flatness, allowing precise positioning for automation. The low-profile wells are optimized for low-volume reactions and fast PCR.
Key Features and Benefits
- Polycarbonate shell — prevents plate warping when thermal cycling in the Roche LightCycler 480
- Extremely uniform wells — reduce well-to-well variability in optical assays performed in real-time PCR
- Black lettering — facilitates well identification
- Rigid shell — ideal for heat sealing methods with instruments such as the PX1 PCR Plate Sealer
Plate Dimensions
Not drawn to scale.
All dimensions in mm, unless otherwise noted.
| Microplate Dimensions | Hard-Shell 96-Well 480 PCR Plates (HSR-99xx) |
| Length at base plane | 127.76 mm |
| Width at base plane | 85.48 mm |
| Height overall | 15.51 mm |
| Well depth | 14.41 mm |
| Well diameter at opening | 5.46 mm |
| Well diameter at bottom of conical section | 3.00 mm |
| Well volume | 200 μl |
| Well spacing | 9.00 mm |
| Well angle | 17.0° |
| Well offset | |
| Left edge to well A1 | 14.38 mm |
| Top edge to well A1 | 11.24 mm |
| Center of A1 to center of A12 | 99.00 mm |
| Center of A1 to center of H1 | 63.00 mm |
Additional Details
- White-well option for increased fluorescent signal strength
- Bar-coded on row A side for convenient database tracking
- Footprint and well spacing match ANSI/SBS standard dimensions
- Reaction volumes of 5–125 µl (200 µl maximum)
- Process sampling and testing to ensure that plates are free of DNase, RNase, and human DNA
Compatible PCR Instrument
- Roche LightCycler 480 Real-Time PCR System
* Hard-Shell plates are covered by one or more of the following U.S. patents or their foreign counterparts owned by Eppendorf AG: U.S. Patent Numbers 7,347,977; 6,340,589; and 6,528,302.