PROTEAN II xi Cell

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Overview

The PROTEAN II xi cell is a large format vertical electrophoresis cell that is used for common electrophoretic techniques such as SDS-PAGE, native electrophoresis, and agarose gel electrophoresis.
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Description

Description

The PROTEAN® II xi cell is a large format vertical electrophoresis cell that is used for the most common electrophoretic techniques such as SDS-PAGE, native electrophoresis, and agarose gel electrophoresis. The PROTEAN II xi cell is available in two sizes to accommodate up to four* 16 x 16 cm or 16 x 20 cm gels.

Key Features

  • Accommodates handcast gels
  • Patented single-screw clamps on the casting stand that exert uniform pressure along the entire length of the glass plates provide a leakproof seal without agarose plugs or grease
  • Central cooling core can be connected to tap water or a cooled recirculating bath, or filled with coolant, providing smile-free patterns with as little as 1.5 L of buffer
  • Choice of glass plates, spacers, and sandwich clamps to cast 2 gel lengths, 16 or 20 cm
  • Ability to cast multiple gradient gels using the PROTEAN II multi-gel casting chamber, the Model 495 gradient former, and the appropriate exponential piston. Use of the gel clip facilitates handling of large gels
  • Notched plates allow up to four 16 x 16 cm or 16 x 20 cm handcast gels to be run simultaneously (not recommended for 2-D applications)
  • Optional frosted glass plates allow high-resolution vertical agarose electrophoresis of nucleic acids
  • Comb conversion screws convert any PROTEAN II xi comb from a standard 25 mm well depth to a 10 mm depth to simplify comb removal

PROTEAN II IPG Conversion Kits for 2-D Applications

Conversion kits expand the capabilities of the PROTEAN II xi cell to include applications such as tube gel IEF and second-dimension SDS-PAGE. To convert the PROTEAN II xi cell into a:

  • PROTEAN xi 2-D cell — use available adaptors and other accessories to accommodate 16 tube gels for IEF applications
  • PROTEAN II XL cell — use the PROTEAN II IPG conversion kit for second-dimension electrophoresis of 17 cm IPG strips

Each cell includes the central cooling core with gaskets, lower buffer chamber, lid with cables, 2 sets of glass plates, 4 sandwich clamps, upper buffer dam, casting stand with gaskets, leveling bubble, and instructions. The 16 cm cells include clamps for running 16 cm gels; the 20 cm cells include clamps for 20 cm gels. Cells without combs and spacers (catalog #165-1801 and #165-1811) allow you to select the spacer thickness and comb that best fit your application. See the Accessories tab for a list of available combs and spacers.

* For higher throughput, the PROTEAN II xi multi-cell provides 6-gel capacity for second-dimension runs.

Ordering

Ordering

items

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(Discontinued) PROTEAN II xi Cell
1651801
Large-format vertical electrophoresis cell, 16 x 16 cm gel size, 4 gel capacity, 2 sets of glass plates, 2 sets of sandwich clamps, casting stand, upper buffer dam, alignment card with leveling bubble; spacers and combs not included
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(Discontinued) PROTEAN II xi Cell
1651802
Large format vertical electrophoresis cell, 16 x 16 cm gel size, 4 gel capacity, includes four 1.5 mm spacers, two 15-well combs, 2 sets of glass plates, 2 sets of sandwich clamps, casting stand, upper buffer dam, alignment card with leveling bubble
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protean-ii-xi-cell-165-1803-thumb.jpg
(Discontinued) PROTEAN II xi Cell
1651803
Large format vertical electrophoresis cell, 16 x 16 cm gel size, 4 gel capacity, includes four 1.0 mm spacers, two 15-well combs, 2 sets of glass plates, 2 sets of sandwich clamps, casting stand, upper buffer dam, alignment card with leveling bubble
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(Discontinued) PROTEAN II xi Cell
1651804
Large format vertical electrophoresis cell, 16 x 16 cm gel size, 4 gel capacity, includes four 0.75 mm spacers, two 15-well combs, 2 sets of glass plates, 2 sets of sandwich clamps, casting stand, upper buffer dam, alignment card with leveling bubble
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protean-ii-xi-cell-165-1812-thumb.jpg
(Discontinued) PROTEAN II xi Cell
1651812
Large format vertical electrophoresis cell, 16 x 20 cm gel size, 4 gel capacity, includes four 1.5 mm spacers, two 15-well combs, 2 sets of glass plates, 2 sets of sandwich clamps, casting stand, upper buffer dam, alignment card with leveling bubble
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(Discontinued) PROTEAN II xi Cell
1651813
Large format vertical electrophoresis cell, 16 x 20 cm gel size, 4 gel capacity, includes four 1.0 mm spacers, two 15-well combs, 2 sets of glass plates, 2 sets of sandwich clamps, casting stand, upper buffer dam, alignment card with leveling bubble
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(Discontinued) PROTEAN II xi Cell
1651814
Large format vertical electrophoresis cell, 16 x 20 cm gel size, 4 gel capacity, includes four 0.75 mm spacers, two 15-well combs, 2 sets of glass plates, 2 sets of sandwich clamps, casting stand, upper buffer dam, alignment card with leveling bubble
1651815
Kit converts PROTEAN II xi cell into a tube gel IEF 2-D system; includes 2 tube gel adaptors, 24 glass tubes (1.5 mm ID, 180 mm length), gaskets, grommets, stoppers
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1651834
Vertical electrophoresis system, includes electrophoresis cell with central cooling core, gel casting stand

Accessories

Accessories

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(Discontinued) Inner Glass Plates
1651823
Pkg of 2, 20 x 20 cm, glass plates for 20 cm PROTEAN II xi and PROTEAN II XL electrophoresis cells, use with outer plate (#165-1824)
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(Discontinued) Outer Glass Plates
1651824
Pkg of 2, 22.3 x 20 cm, glass plates for 20 cm PROTEAN II xi and PROTEAN II XL electrophoresis cells, use with inner plate (#165-1823)
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1651825
Pkg of 2, 16 x 20 cm, glass plates for agarose gels, for 16 cm PROTEAN II xi electrophoresis cell only, use with outer plate (#165-1822)
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1651827
Pkg of 2, 16 x 20 cm, 16 cm bevel length, for 2-D tube gel procedures, for PROTEAN II xi 2-D electrophoresis cell, use with outer plate (#165-1822)
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1651832
Pkg of 1, 16 x 20 cm, 16 cm bevel length, glass plate for double-up procedures, for 16 cm PROTEAN II xi electrophoresis cell only
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1651859
Pkg of 10 each comb conversion and standard screws, for conversion of 2 PROTEAN II xi combs from 25 mm to 10 mm well depth (agarose gels)

Documents

Documents

Number Description Options
4106107
Instruction Manual, PROTEAN® II xi Cell IPG Conversion Kit, Rev B
1760
PROTEAN® II xi and XL Cells PIS, Rev F
3095
2-D Electrophoresis: Tools for Rapid, High-Resolution Protein Separations Brochure, Rev B