NGC Detector Modules and Components

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Overview

The single-wavelength, multi-wavelength, conductivity, and pH detectors enable real-time sample monitoring for high-sensitivity monitoring of complex samples without background interference.
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Description

Description

The family of NGC Medium-Pressure Chromatography Systems can be personalized with the addition of specific modules pertinent to your application and throughput needs. Different detectors can be added according to the type of detection needed: use the single-wavelength detector for identifying nucleotides and proteins, and the multi-wavelength detector for distinguishing proteins, peptides, nucleic acids, and chromophores.

The NGC Scout 10, NGC Quest 10 , and NGC Quest 100 Systems include the single-wavelength detector module while the NGC Scout 10 Plus, NGC Quest10 Plus, NGC Quest 100 Plus, NGC Discover 10, and NGC Discover 10 Pro Systems include the multi-wavelength detector module. Both of these detector modules have an integrated conductivity module for monitoring salt gradients.

The NGC pH Valve Module includes a pH electrode, for accurate inline pH monitoring during the run (pH range 1–14). The pH valve is capable of directing or bypassing the flow of sample to the pH electrode and is controlled via ChromLab Software. The NGC Scout and NGC Discover Systems include the pH valve module, which is available as an add-on for all NGC Quest Systems.

Features and Benefits of NGC Chromatography Detector Modules

  • Both single- (UV) and multi-wavelength (UV/Vis) detectors can be used with interchangeable flow cells with path lengths of 2, 5, and 10 mm
  • Both single- and multi-wavelength detectors have an integrated conductivity module for monitoring salt gradients
  • pH valve module features integrated calibration, bypass, and pH detection ports

Applications and Uses of NGC Chromatography Detector Modules

  • Single-wavelength (UV) detector module for detecting absorbance of nucleotides (255 nm) or proteins (280 nm)
  • Multi-wavelength (UV/Vis) detector module for monitoring up to four wavelengths simultaneously between 190 and 800 nm and detecting the absorbance of proteins, peptides, nucleic acids, and chromophores
  • pH valve module for inline monitoring of buffer pH during separation of sensitive proteins

More Information

Additional modules may be added to your NGC Chromatography System to suit your application and throughput needs. For example, the sample pump module enables accurate and large volume sample application, the buffer blending valve module enables automatic inline buffer preparation and generation of pH gradients for quick pH scouting, and the column switching valve module attaches multiple columns for your applications or for column scouting.

ChromLab Software makes it easy to incorporate the added modules into fluidic schemes and methods or to operate and monitor their individual status during automated, complex separations. Additional modules are described on the NGC Chromatography Modules and Accessories page.

Ordering

Ordering

items

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7884008
Pkg of 1, includes UV/conductivity detector kit with necessary tubing and fittings, for nucleotide and protein detection, and salt gradient monitoring
ngc-ph-valve-module-SKU-788-4011-thumb.jpg
7884011
Pkg of 1, contains the pH valve kit, includes pH probe, tubing, fittings, for accurate inline pH measurement
12010343_thum.jpg
12010343
Pkg of 1, includes UV/Vis and conductivity detector kit with necessary tubing and fittings, for protein, peptide, nucleic acid, and chromophore detection

Accessories

Accessories

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7885056
Pkg of 1, replacement conductivity monitor
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7885026
Pkg of 1, for accurate pH monitoring (pH 1–14) during purifications performed to 200 ml/min, for use with the pH valve
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(Discontinued) NGC™ pH Valve Cap
7885027
Pkg of 1, contains a pH valve cap, for use when the pH valve is removed, not required, or not inline
ngc-uv-flow-cell-10-mm-SKU-788-5023-thumb.jpg
7885022
Pkg of 1, includes 2 mm UV flow cell for use with NGC Chromatography Systems for preparative applications. Compatible with the NGC Single-Wavelength (7884008) and Multi-Wavelength (7884009) detector modules.
ngc-uv-flow-cell-5-mm-SKU-788-5024-thumb.jpg
7885024
Pkg of 1, includes standard 5 mm UV flow cell for use with NGC Chromatography Systems. Compatible with the NGC Single-Wavelength (7884008) and Multi-Wavelength (7884009) detector modules.
ngc-uv-flow-cell-10-mm-SKU-788-5023-thumb.jpg
7885023
Pkg of 1, includes 10 mm UV flow cell for use with NGC Chromatography Systems for analytical applications. Compatible with the NGC Single-Wavelength (7884008) and Multi-Wavelength (7884009) detector modules.
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7885000
Pkg of 1, 255/280 nm LED lamp replacement for single-wavelength UV detectors
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7885001
Pkg of 1, UV lamp replacement for multi-wavelength detectors
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7885002
Pkg of 1, visible lamp replacement for multi-wavelength detectors

Documents

Documents

Number Description Options
6286
NGC Chromatography Systems Brochure
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6326
NGC Chromatography Systems Module Selection Guide
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6778
Recommended Flow Rates for Tubing, UV/Vis Flow Cells, and Mixer Barrels on NGC™ Chromatography Systems Guide, Ver A
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