NGC 10 Medium-Pressure Chromatography Systems

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Overview

The preconfigured NGC 10 Chromatography Systems meet a range of throughput and application needs. Each system can be easily upgraded. All configurations share a common software platform. For analytical and preparative chromatography.

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Description

Description

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The family of NGC Medium-Pressure Chromatography Systems fit your throughput requirements when purifying biomolecules for analytical- and preparative-scale applications. Each system is composed of interchangeable modules that confer different system capabilities.

The preconfigured NGC Liquid Chromatography Systems listed below are designed with increasing automation and throughput to serve a wide range of laboratory needs:

  • NGC Quest 10 Plus Systems (7880003) — for separating biomolecules at high resolution using accurate gradients
  • NGC Scout 10 Plus Systems (7880007) — for medium throughput applications requiring rapid scouting of protein purification conditions with automated gradients and buffer preparation
  • NGC Discover 10 (7880009) and NGC Discover 10 Pro Systems (7880011) — for higher throughput applications with rapid, robust automation and a need for method development

For an overview of the features and capabilities of the four NGC Chromatography Systems, view the NGC 10 System comparison chart and watch the NGC System Tour.

The NGC Systems are composed of interchangeable modules that confer different system capabilities. Browse the module selection guide to help you decide which system is best for you, or use our online tool to configure your personalized NGC Instrument.

Multidimensional (Multi-D) Chromatography with NGC Systems

Multi-D chromatography is a feature of the NGC Systems that automates multistep purifications using complementary strategies to isolate your product of interest at high yields and purity levels run after run.

Compared to traditional sequential chromatography workflows, which require running single columns sequentially, Multi-D sets up an automated workflow and consolidates day-long methods to hour-long protocols without requiring the intervention of users, allowing them to save valuable time.

 

Resources for Converting to Automated Multi-D Chromatography

 
  • Automated Multi-Column Protein Purification with the NGC System

    This whiteboard animation video provides a brief introduction to Bio-Rad’s automated multi-column purification options (Multi-D purification) on Bio-Rad's NGC Preparative Chromatography System.

  • The Advantages of Multidimensional (Multi-D) Chromatography

    Watch the Multi-D Webinar to learn more about automating purifications, optimizing methods, and creating multidimensional chromatography method templates.

Features and Benefits of the NGC 10 ml Chromatography Systems

Flexible, customizable chromatography systems to suit both your application and workflow needs:

  • The guided fluidics selection tool (patent pending), coupled with the module discovery system, enables a single-click application-based system setup through automated module function recognition
  • The Tier Rotate Feature allows optimal placement of valves and detectors to minimize the flow path
  • Compact footprint — fits in a refrigerator, lab bench, or coldroom
  • Point-to-Plumb Feature shows the step-by-step plumbing of the system using LEDs
  • ChromLab Software provides powerful system control, automated methods, and analysis options
  • Multi-D setup automates multistep purifications and completes complex separation protocols without user involvement

Applications and Uses of Medium-Pressure Chromatography Systems

Other NGC Chromatography Systems

Bio-Rad also offers NGC Chromatography Systems with integrated 100 ml system pumps and NGC Fraction Collector as well as a variety of chromatography columns and resins.

Compatible Chromatography Columns

Designed specifically for NGC Chromatography Systems, these prepacked high-resolution columns work at high pressures and are optimized for the separation of biomolecules at fast flow rates.

 

More Information

 

Upgrade your NGC Chromatography System by adding more modular components to match your changing throughput and application requirements:

See how the NGC System benefits University of California researchers by increasing throughput and making it easier for students to become experts at protein purification.

Ordering

Ordering

items

Use the filters below to refine results!

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7880001
Includes 10 ml/min pumps, single-wavelength (UV) and conductivity detection, and automated sample injection, for the purification of proteins
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7880003

Includes 10 ml/min pumps, multi-wavelength (UV/Vis) and conductivity detection, and automated sample injection, for the purification of proteins

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7880005
Includes 10 ml/min pumps, single-wavelength (UV) and conductivity detection, buffer blending options, for rapid pH scouting during sample purification
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7880007
Includes 10 ml/min pumps, multi-wavelength (UV/Vis) and conductivity detection, buffer blending options, for rapid pH scouting during the purification of proteins, peptides, and nucleic acids
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7880009
Includes 10 ml/min pumps, multi-wavelength (UV/Vis) and conductivity detection, expanded scouting options, large-sample injection, and column switching of up to 5 columns, for method development
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7880011
Includes 10 ml/min pumps, multi-wavelength (UV/Vis) and conductivity detection, expanded scouting options, large-sample injection, outlet valve, and column switching of up to 5 columns, for method development

Accessories

Accessories

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7410002

100/240 V, fraction collector, includes power cord, rack set F1 (2 x flatpack, 13 mm), BioFrac Diverter Valve, fittings kit

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(Discontinued) Computer
7884015

PC for ChromLab Software and database, communicates with and stores data generated by the NGC System

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7884016

Pkg of 1, enables analog to digital signal conversion and connection to external detectors and third party autosamplers such as the C-96 Autosampler

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7885003
The NGC System Pump Maintenance Kit includes pump head seals, wash behind seals, wash behind check valves, check valves, O-rings, and small and large disks for servicing one NGC Chromatography F10 pump.
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7885026

Pkg of 1, for accurate pH monitoring (pH 1–14) during purifications performed to 200 ml/min, for use with the pH valve

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12012531
Pkg of 1, includes a 10 mm UV flow cell with an integrated conductivity monitor compatible with the NGC Multi-Wavelength II Detector Module (12010343) for analytical applications.
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12012532
Pkg of 1, includes a 5 mm UV flow cell with an integrated conductivity monitor compatible with the NGC Multi-Wavelength II Detector Module. (12010343)
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12012533
Pkg of 1, includes a 2 mm UV flow cell with an integrated conductivity monitor compatible with the NGC Multi-Wavelength II Detector Module (12010343) for preparative applications.

Fittings

Fittings

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(Discontinued) NGC Fittings Kit
7884017

Pkg of 1, kit includes multiple fittings, fittings maker, fittings tightener, ferrules, nuts, screwdrivers, pump head keys, and syringes required to set up, prepare, and prime NGC Systems

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7320113

Low-pressure system fittings kit, includes 1/4–28 female to male and 1/4–28 female to female luers to connect 1 cartridge to BioLogic DuoFlow and NGC Systems

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(Discontinued) Tefzel Plug
7500563

Pkg of 5, 1/4–28 male connection, to plug unused ports on valves and columns, for use with BioLogic DuoFlow and NGC Systems

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7885009
Pkg of 4 (qty 2 pairs — each with one 1/4-28 F to 10-32 M adaptor and one 1/4-28 M to 10-32 F adaptor)
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7500565

Pkg of 2, fittings to connect one Econo-Column (luer) Column to BioLogic DuoFlow and NGC Systems

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7885010
Pkg of 10, includes five slip luer F to 10-32 M adaptors and five quick-connect luer M to 10-32 F adaptors
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(Discontinued) Tefzel Cap
7500559

Pkg of 5, Tefzel caps, 1/4–28 female connection, to plug unused tubing, for use with BioLogic DuoFlow and NGC Systems

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7500568

Fittings kit, includes 2 nuts and 4 ferrules to connect UNO Column to an HPLC system, for use with BioLogic DuoFlow and NGC Systems

Modules

Modules

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12010343

Pkg of 1, includes UV/Vis and conductivity detector kit with 5 mm flow cell, necessary tubing, and fittings, for protein, peptide, nucleic acid, and chromophore detection

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7884008
Pkg of 1, includes UV/conductivity detector kit with necessary tubing and fittings, for nucleotide and protein detection, and salt gradient monitoring
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7884007

Pkg of 1, kit includes necessary tubing, fittings, and a sample injection port, for automated application of small sample volume samples

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7884006

Pkg of 1, kit includes necessary tubing and fittings, for automated switching between multiple buffers and samples during method development

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7884013

Pkg of 1, kit includes necessary tubing and fittings for automated fraction collection of large-volume fractions with up to 12 vessels

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7884010
Pkg of 1, kit includes necessary tubing and fittings, for inline buffer preparation and pH gradient generation for quick pH scouting
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7884012
Pkg of 1, kit includes the necessary tubing and fittings to accommodate up to 5 columns, for column scouting and reverse flow applications using 10 ml NGC Chromatography Systems
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7884004

Pkg of 1, includes 100 ml/min sample pump kit with the necessary tubing and fittings, for automated large-volume sample application

Documents

Documents

Number Description Options
3359
Inline Dilution for Antibody Purification on NGC Chromatography Systems Flier
3232
Scale Separation: Optimizing the Bio-Rad NGC Chromatography System for Low Volume Size Exclusion Chromatography
3229
Optimizing the NGC Chromatography System for Cryo-EM Applications Quick Guide
6456
Connectors for Using Bio-Rad and Cytiva Columns on NGC Chromatography Systems Quick Guide
7260
Microbial Challenge and Sanitization of the NGC Discover 10 Pro System Using Sodium Hydroxide
7194
Integrating the CETAC MVX-7100 Autosampler with the NGC Chromatography System Protocol, Ver A
7208
NGC Chromatography System Connection Guide for Wyatt Instrumentation Protocol
[ Add to Cart (Free) ]
3211
Purification of Cas9 Nuclease with a Two-Step Chromatographic Process Application Note
7195
Integrating the CETAC MVX-7100 Autosampler with the NGC Chromatography System Quick Guide, Ver A
7205
NGC Liquid Chromatography System Quick Guide
7137
A Model System for Fast Screening of Optimal Protein Purification Conditions
7031
Integrating the ALIAS Autosampler with the NGC Chromatography System Protocol
6297
Foresight Prepacked Plates and Columns Product Information Sheet
6286
NGC Chromatography Systems Brochure
[ Add to Cart (Free) ]
7209
Chemical Compatibility with the NGC Chromatography System Flier
[ Add to Cart (Free) ]
6326
NGC Chromatography Systems Module Selection Guide
[ Add to Cart (Free) ]
10000049090
NGC Chromatography Systems and ChromLab Software Installation Guide Version 6.1
10000049091
NGC Chromatography Systems and ChromLab Software Instrument Guide Version 6.1
10000049092
NGC Chromatography Systems and ChromLab Software User Guide Version 6.1
6879
Increasing Thoughput of a Tandem mAb Purification by Adding a High-Volume Autosampler to the NGC Chromatography System
6896
Integrating the CETAC ASX-560 Autosampler with the NGC Chromatography System
6608
NGC Chromatography Systems for Protein Purification in Drug Discovery and Development Brochure
[ Add to Cart (Free) ]
6963
NGC Fraction Collector Brochure
6674
NGC Chromatography Systems Multidimensional (Multi-D) Plumbing Guide
6377
Chromatography Success Guide
6701
Multidimensional (Multi-D) Chromatography Success Guide, Ver B
7277
Determination of Molecular Weight and Disperity of Antibody Samples Using Bio-Rad's NGC Chromatography System and Wyatt's MALS and RI Detectors
6694
Advantages of Multidimensional (Multi-D) Chromatography Using the NGC Chromatography System over Traditional Sequential Chromatograph
6725
From Optimization to Automation: Multidimensional (Multi-D) Histidine-Tag Protein Purification, Ver C
6745
Automated mAb Workflows: Combining Multidimensional (Multi-D) Purifications with Product Analysis, Ver C
6297
Foresight Prepacked Plates and Columns Product Information Sheet
6494
Protein Purification Workflow with the NGC Chromatography System and Stain-Free Gels Brochure
[ Add to Cart (Free) ]
6601
Enhancing the Flexibility of the NGC™ Chromatography System: Addition of a Refractive Index Detector for Wine Sample Analysis, Rev A
6529
Protein Purification and Crystallization of Diabetes Related Peroxisome Proliferator–Activated Receptor-γ (PPARγ), Rev A
6769
From Optimization to Automation: Multidimensional (Multi-D) Histidine-Tag Protein Purification (Poster), Ver A
6770
Automated mAb Workflows: Combining Multidimensional (Multi-D) Purifications with Product Analysis (Poster), Ver A
6805
Proven Method for Confirming Protein Purity and Yield Brochure
[ Add to Cart (Free) ]
6843
NGC Chromatography System Applications Publication List
[ Add to Cart (Free) ]
6675
ChromLab Software Product Information Sheet
3251
Cas9 Nuclease Preparation Using EconoFit Columns and the NGC Chromatography System Application Note
3352
NGC Chromatography System for Low-Volume, High-Resoution Size Exclusion Chromatography Flier