Gene Pulser® Electroporation Buffer

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Gene Pulser Electroporation Buffer

Overview

Data Using the Gene Pulser MXcell™ System

Optimization of electroporation conditions. Human primary fibroblast cells denoted as FS (foreskin) were used to compare a square waveform with varying voltages (top) to an exponential waveform with varying capacitance values (bottom) in a 96-well plate. Although the exponential waveform resulted in good data overall, the square waveform resulted in the highest transfection efficiency and cell viability when compared side by side on the same plate. RLU, relative luminescence units.

Data Using the Gene Pulser Xcell™ System

Gene Pulser® electroporation buffer produces optimal results in various electroporation systems. Gene Pulser Xcell™, Gene Pulser II, and a competitor's electroporation instrument were used to demonstrate the Gene Pulser electroporation buffer's flexibility. CHO cells were transfected with a scrambled siRNA (green) or an siRNA targeting luciferase (blue). Similar transfection efficiencies were observed across platforms for siRNA delivery.

Gene Pulser electroporation buffer outperforms buffers traditionally used for electroporation when using either square or exponential waveforms. Transfection conditions were optimized in different cell types. For COS-7 cells (top), a square-wave protocol was optimal, whereas for K562 suspension cells (bottom) an exponential-decay protocol was optimal. Under these optimized conditions, Gene Pulser electroporation buffer yielded significantly higher transfection efficiencies than phosphate buffered saline (PBS) and Opti-MEM medium (Invitrogen Corporation), both of which are commonly used for electroporation. RLU, relative luminescence units.

Cell Survival

Cell survival under a range of electroporation conditions. Post-electroporation survival of neutrophils was assayed after 24 hr in culture by counting the number of intact cells that excluded trypan blue, and dividing by the number of neutrophils initially transfected. Cell survival was substantially reduced at 300 V and 1,000 µF.

Transfection Protocol Library


The Transfection Protocol Online Library contains protocols obtained from the literature, developed by Bio-Rad scientists, or submitted by scientists like you. browse protocols to view our library and find your starting point.

Bio-Rad Laboratories has helped scientists deliver molecules into cells for over 35 years. Instruments, including the Gene Pulser® series of electroporators, the MicroPulser electroporator, and the Helios® gene gun and PDS-1000/He biolistic systems, have been used successfully to transfect prokaryotic and eukaryotic cells, including plant and animal cells. These transfection instruments are cited extensively in the literature. Our chemically mediated methods include lipid-based transfection reagents — TransFectin lipid reagent for delivery of plasmid DNA and siLentFect lipid reagent for siRNA delivery.

Gene Pulser electroporation buffer is formulated to improve cell transfection rates by minimizing cell mortality while ensuring highly efficient delivery of nucleic acids.


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Description

Gene Pulser electroporation buffer is formulated to improve electroporation by minimizing cell mortality while ensuring highly efficient delivery of nucleic acids. This universal buffer is an excellent tool for electroporating mammalian cells, including primary cells and difficult-to-transfect cells.

Key Features

  • Works with most electroporators, including the Gene Pulser MXcell, Gene Pulser Xcell, and Gene Pulser II electroporation systems
  • Delivers siRNA, DNA, and other biomolecules into primary cells and difficult-to-transfect cell lines
  • Improves efficiency of transfection and maintains cell viability after electroporation
  • Can be used for transfection in single cuvettes or in multiwell electroporation plates

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Gene Pulser Electroporation Buffer

1652676
10 x 1.8 ml, electroporation buffer for mammalian, primary, and difficult-to-transfect cells, for use with any Gene Pulser electroporation system

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Gene Pulser Electroporation Buffer

1652677
30 ml, electroporation buffer for mammalian, primary, and difficult-to-transfect cells, for use with any Gene Pulser Electroporation System

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Documents

Number Description Options
10009178 Instruction Manual, Gene Pulser Electroporation Buffer Reagent, Rev B Click to download
5582 Gene Pulser Electroporation Buffer Product Information Sheet, Rev A Click to download
5596 Quick Guide, Gene Pulser Electroporation Buffer Optimization, Rev B Click to download
5598 Gene Pulser MXcell Electroporation System Flier, Rev B Click to download
5641 The Gene Pulser MXcell Electroporation System Delivers Consistent Results Required for Optimizing Delivery Protocols, Rev A Click to download
5603 Optimization of Electroporation Using Gene Pulser Electroporation Buffer and the Gene Pulser MXcell Electroporation System, Rev A Click to download
5622 Optimization of Electroporation Conditions With the Gene Pulser MXcell Electroporation System, Rev A Click to download
5686 Optimization of Electroporation Conditions for Jurkat Cells Using the Gene Pulser MXcell Electroporation System, Rev A Click to download
5687 Transfection of Mammalian Cells Using Preset Protocols on the Gene Pulser MXcell Electroporation System, Rev A Click to download
5720 Transfection of Neuroblastoma Cell Lines Using the Gene Pulser MXcell Electroporation System, Rev A Click to download
5704 Electroporation Conditions for Chinese Hamster Ovary Cells Using the Gene Pulser MXcell Electroporation System, Rev A Click to download
5733 Transfection of Chinese Hamster Ovary-Derived DG44 Cells Using the Gene Pulser MXcell Electroporation System, Rev A Click to download
5774 Delivery of siRNA by Electroporation Into Primary Human Neutrophils Using the Gene Pulser MXcell System, Rev A Click to download
5778 Electroporation Parameters for Transfection of HL-60 Leukocytic Cell Line With siRNA Using the Gene Pulser MXcell System, Rev B Click to download
5842 Optimization of Electroporation Conditions for Two Different Burkitt Lymphoma Cell Lines Using the Gene Pulser MXcell System, Rev B Click to download
5904 Transfection of Mouse and Human Embryonic Stem Cells by Electroporation Using the Gene Pulser Mxcell™ System, Rev A Click to download

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