ChemiDoc MP Imaging System

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ChemiDoc MP System

Overview

Stain-Free Western Blotting
Fluorophore Compatibility Chart

Fluorophores
      Starbright™  
DyLight 488 DyLight 550 DyLight 650 DyLight 680 DyLight 800
Alexa Fluor 488 Alexa Fluor 546 Alexa Fluor 647 Alexa--a Fluor 680 Alexa Fluor 790
Cy2 Cy3 Cy5 Cy5.5 Cy7
  Rhodamine SYPRO Ruby IRDye 680 IRDye 800
Imaging System Selection Guide

  ChemiDoc MP ChemiDoc Touch GelDoc GO Gel Doc XR+ GS-900
ChemiDoc
MP
ChemiDoc
GelDoc Go
Gel Doc
XRS+
GS-900
Nucleic Acid Detection
Ethidium Bromide
SYBR Green I and SYBR Safe Dyes
Fast Blast DNA Stain
Protein Staining, 1-D Gels
Stain-Free Gels
Coomassie Blue Stain
Silver Stain
SYPRO Ruby, Flamingo, and Oriole Gel Stains
Protein Staining, 2-D Gels
Coomassie Blue Stain
Silver Stain
SYPRO Ruby, Flamingo, and Oriole Gel Stains
Cy2, Cy3, Cy5 Label
Blot Detection
Multiplex fluorescence
Immun-Star 
Chemiluminescence
Stain-free blots
Colorimetric blots
SYPRO Ruby Protein Blot Stain*

*  Optimal with low fluorescence PVDF membrane.

Use the ChemiDoc MP Imaging System for imaging of gels and blots. Detect multiplex fluorescence, chemiluminescence, and a wide range of different stains.
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Ordering Information


Description

Request a Demo
Stain Free Enabled

What is Stain-Free Technology?

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The ChemiDoc MP Imaging System is a full-feature instrument for imaging and analyzing gels and western blots. It is designed to address multiplex fluorescent western blotting, chemiluminescence detection, general gel documentation applications, and stain-free technology imaging needs.

Complete Capabilities: Superior Images

Stain-Free
Total Protein Normalization

Learn how to properly design a quantitative western blotting experiment and faithfully compare protein expression levels, without concern for changing or overexpressed HKP’s.
See Data »

Chemiluminescence
Replaces Film

Save money on consumables and reduce environmental waste without compromising on performance. Get the sensitivity of film and the convenience of digital documentation.
See Data »

Multiplex Fluorescence
3 Colors

Save sample, avoid the errors commonly associated with stripping, reprobing, and cutting blots and answer more complex biological questions by optimizing your experiments to fluorescence.
See Data »

Features and Benefits

  • All-in-one flexible imaging — get precise, reproducible fluorescence, chemiluminescence, and colorimetric gel and blot detection, analysis and documentation in a single system
  • Improve your imaging capabilities with the ChemiDoc MP — benefit from the advantages of using a wider range of fluorescent reporters (RGB, far red, and near infrared) including StarBright Fluorophores; see the fluorophore compatibility chart
  • Multiplex fluorescent western blotting — detect up to three proteins simultaneously and eliminate the need for stripping and reprobing
  • Ease of use — features include automatic selection of optimal light source by application, auto focus, auto exposure, and preview features ensure optimal images. Learn in minutes with on screen help
  • Stain-free protein normalization — stain-free imaging permits the normalization of bands to total protein in both in gels and blots; eliminates the need for housekeeping proteins
  • Sample Trays and Smart Tray Technology — three sample trays available to cover diverse imaging applications. Smart Tray Technology automatically recognizes the application-specific trays and adjusts imaging parameters and software options accordingly
  • Installation/Operational Qualification kit available for ensuring installation and calibration is properly executed for highly regulated laboratory environments.

Performing Stain-Free western blotting using the ChemiDoc MP System provides the ability to rapidly assess electrophoresis and blot transfer quality prior to blot processing, to perform total protein normalization and multiplexing using a wide rance of fluorophores.

Fluorophore reference poster for western blotting and gel imaging

Request a Free Fluorophore Reference Poster

Determine the best fluorophore for maximum sensitivity and high signal-to-noise ratio in your next experiment using this poster.

Applications and Uses

  • Electrophoresis — protein, including stain-free, and nucleic acid gel imaging and documentation
  • Blotting — identify and quantify proteins, nucleic acids, protein-ligand interactions, etc. with fluorescence, chemiluminescence, or colorimetric detection

Included Components

  • Internal computer with Image Lab Touch Software
  • 12” touch screen display
  • Image Lab Software to install on your computer
  • Blot/UV/Stain-Free Sample Tray for Bio-Rad stain-free gels and blots, fluorescence multiplexing, chemiluminescence detection, ethidium bromide and SYPRO Ruby (12003028)

Mini and Midi ChemiDoc MP Stain-Free Western Blotting also include:

  • ChemiDoc MP Imaging System
  • Mini-PROTEAN Tetra Cell, 4-gel (1658004) or Criterion Cell (1656001) Electrophoresis Chamber
  • PowerPac Basic Power Supply (1645050)
  • Trans-Blot Turbo Transfer System (1704150)
  • 4–20% Mini-PROTEAN TGX Stain-Free or 4–20% Criterion TGX Stain-Free Protein Gels
  • Buffers, Nitrocellulose Transfer Packs, Immun-Blot LF PVDF Membranes, and other consumables

Optional Components

  • White Sample Tray for Coomassie Blue, copper, silver, and zinc stains (12003026)
  • Blue Sample Tray for nucleic acid applications with GelGreen or any SYBR Stain (12003027)
  • Holder for ChemiDoc Sample Trays and UV Shield (1708377)
  • Gel Alignment Templates for ChemiDoc MP and ChemiDoc Imaging Systems (12003915)
  • UV Safety Shield for ChemiDoc MP and ChemiDoc Imaging Systems (12003914)

Configuration Options

Product
Catalog Number
ChemiDoc MP Imaging System
17001402
ChemiDoc MP Stain-Free Western Blotting Workflow for Mini Gels
17001395
ChemiDoc MP Stain-Free Western Blotting Workflow for Midi Gels
17001389

Note: Also available are the ChemiDoc Imaging System (17001401), ChemiDoc Stain-Free Western Blotting Workflows for Mini Gels (17001403), and ChemiDoc Stain-Free Western Blotting Workflows for Midi Gels (17001390), which have a more limited fluorescence capability than ChemiDoc MP Systems. These systems can be upgraded to a broader spectrum (full RGB, far red, and near IR) to enable fluorescence multiplexing with the ChemiDoc to ChemiDoc MP Upgrade Kit (12003263).

Related Categories

ChemiDoc MP Imager

Important Information

The ChemiDoc MP Imaging System featured on this page replaces our classic model of the ChemiDoc MP Imager, which is being discontinued. However, we will continue to provide support for customers with the discontinued model.


Specifications

Automation Capabilities  
Smart Tray Technology™ Automatic recognition of application-specific tray and adjustment of imaging parameters and software options
Autofocus Precalibrated focus for any zoom setting or sample height
Auto-exposure Two user-defined modes (rapid or optimal auto-exposure) for all blot and gel imaging applications
Image flat fielding Dynamic; precalibrated and optimized for each application
Hardware  
Touch-screen functionality Multitouch capability (2 points) 12.1" display
Maximum image area (W x H) 21 x 16.8 cm
Detector Cooled CCD, 6 megapixels
Dynamic range >4 orders of magnitude
Illumination sources Trans-UV, 302 nm excitation
Epi-white
Trans-white (requires White Sample Tray)
Trans-blue, 450−490 nm excitation (requires Blue Sample Tray)
Epi-blue, 460−490 nm excitation
Epi-green, 520−545 nm excitation
Epi-red, 625−650 nm excitation
Epi-far red, 650−675 nm excitation
Epi-near IR, 755−777 nm excitation
Filter and application 590/110 nm standard filter to perform protein and DNA gel and blot imaging
Chemiluminescence filter
518–546 nm filter for blue-excitable fluorophores and stains
577–613 nm filter for green-excitable fluorophores and stains
675–725 nm for red-excitable fluorophores and stains
700–730 nm filter for far red-excitable fluorophores and stains
813–860 nm for near IR-excitable fluorophores and stains
Data output 16-bit or 8-bit: SCN, TIFF, JPEG files
Instrument weight 35 kg (78 lb)
Instrument size (L x W x H) 61 x 51 x 53 cm
Operating voltage 100–250 V
Operating temperature 10–28°C
Operating humidity 10–85% relative humidity (non-condensing)

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ChemiDoc™ MP Imaging System

12003154
The ChemiDoc MP Imaging System is a full-feature, best-in-class instrument for imaging and quantifying nucleic acid and protein in gels and western blots. It is capable of imaging fluorescent western blots (RGB, far-red, near-IR), chemiluminescent western blots, all common nucleic acid and protein gel stains, plus stain-free gels. Includes internal computer, 12" touch-screen display, camera, Image Lab™ Touch Software, and Image Lab Software

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Accessories

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White Sample Tray for ChemiDoc™ MP/ChemiDoc Imaging Systems

12003026
Pkg of 1, sample tray for gels and blots stained with Coomassie Blue, copper, silver, or zinc stains

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$1,126.00 
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Blue Sample Tray for ChemiDoc™ MP/ChemiDoc Imaging Systems

12003027
Pkg of 1, sample tray for nucleic acid applications with GelGreen or any SYBR® Stain

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$1,126.00 
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Blot/UV/Stain-Free Sample Tray for ChemiDoc™ MP/ChemiDoc Imaging Systems

12003028
Pkg of 1, sample tray for imaging chemiluminescence and fluorescence blots, stain-free gels and blots, and gels stained with ethidium bromide, SYPRO Ruby, Oriole, or SYBR® Stains

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$1,126.00 
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UV Safety Shield for ChemiDoc™ MP/ChemiDoc Imaging Systems

12003914
Pkg of 1, UV safety shield

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$309.00 
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ChemiDoc™ and ChemiDoc MP Imaging System Installation/Operational Qualification Kit

12005137
This kit ensures your ChemiDoc and ChemiDoc MP Instruments are properly installed and calibrated according to the technical specifications for optimal performance according to regulatory guidelines

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$4,917.00 
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XcitaBlue Viewing Goggles

1708185
Pkg of 1, goggles for use with SYBR Safe dyes and Blue Trays for use with ChemiDoc and GelDoc Imaging Systems

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$28.00 
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Holder for Sample Trays and UV Shield

1708377
Pkg of 1, imaging tray holder, holds 3 sample trays and 1 UV shield for ChemiDoc and GelDoc Imaging Systems; trays and shield not included

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$77.00 
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Gel Alignment Templates for ChemiDoc™ MP and ChemiDoc Imaging Systems

12003915
Pkg of 2, alignment templates for use with ChemiDoc MP and ChemiDoc Imaging Systems

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$97.00 
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Mitsubishi Thermal Printer

1708089
Optional thermal printer, for use with Bio-Rad imaging systems

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$1,992.00 
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Standard 302 nm UV Lamps

1708097
Pkg of 6, 302 nm replacement UV lamps for all ChemiDoc™ and Gel Doc™ Imaging Systems

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$396.00 
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Documents

Number Description Options
6356 Traditional vs. Stain-Free Western Blotting Click to download
6253 Stain-Free Western Blotting Brochure Click to download [ Add to Cart (Free) ]
6390 General Stain-Free Western Blotting Protocol Click to download [ Add to Cart (Free) ]
7051 Imaging Systems Family Flier Click to download [ Add to Cart (Free) ]
6873 ChemiDoc MP Imaging System Brochure Click to download
6936 ChemiDoc MP Imaging System Flier, Ver A Click to download
10000062126 ChemiDoc and ChemiDoc MP Imaging Systems with Image Lab Touch Software Use Guide Version 3.0.1 (English) Click to download
100000131171 ChemiDoc and ChemiDoc MP Imaging Systems with Image Lab Touch Software Use Guide Version 3.0.1 (Simplified Chinese) Click to download
6427 Bio-Rad Stain-Free Western Blotting Click to download [ Add to Cart (Free) ]
10000060300 Acquiring an Image with ChemiDoc™ and ChemiDoc MP Imaging Systems Quick Guide, Ver A Click to download
10000060301 Acquiring a Multichannel Image with the ChemiDoc™ and ChemiDoc MP Imaging Systems Quick Guide, Ver B Click to download
6493 Bio-Rad’s Host Cell Protein (HCP) Workflow Quick Guide Click to download [ Add to Cart (Free) ]

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