hFAB™ Rhodamine Housekeeping Protein Fluorescent Primary Antibodies

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hFAB Rhodamine Housekeeping Protein Fluorescent Primary Antibodies allow one-step detection of common housekeeping proteins like actin, tubulin, and GAPDH in your samples. These anti-actin, anti-tubulin, and anti-GAPDH antibodies are directly labeled with a rhodamine derivative (Ex_max/Em_max = 530 nm/580 nm). The hFAB Rhodamine Antibodies were generated with Human Combinatorial Antibody Library (HuCAL) Technology, providing exceptional specificity.

These housekeeping protein antibodies can be used for protein normalization when the protein loading amounts are in the linear dynamic range. hFAB Rhodamine Antibodies can be imaged with the ChemiDoc MP Imaging System, and can be used in multiplex experiments with StarBright™ Blue 700 Secondary Antibodies as well as other traditional fluorescent antibodies, permitting detection of multiple target proteins and the housekeeping protein in a single blot.

Features and Benefits

• Simple — No requirement for a secondary antibody; one-step detection of housekeeping proteins using highly cross-adsorbed antibodies for high specificity and sensitivity
• Versatile — Compatible with use of a wide range of other fluorophores including StarBright Blue 700 and DyLight 800 Fluorophores for multiplex western blotting
• Unique — HuCAL Technology eliminates cross-reactivity with primary/secondary antibodies from other species
• Diverse — Antibodies have been tested to detect human or mouse housekeeping proteins

Related Products

• Primary Antibodies
  • PrecisionAb™ Validated Antibodies
    • Actin for Chemiluminescent Western Blotting
    • Tubulin for Chemiluminescent Western Blotting
    • GAPDH for Chemiluminescent Western Blotting
    • Precision Plus Protein Prestained Protein Standards
• Secondary Antibodies
  • StarBright Blue 520 Fluorescent Secondary Antibodies
  • StarBright Blue 700 Fluorescent Secondary Antibodies
  • DyLight 800 Secondary Antibodies
  • HRP Secondary Antibodies
• ChemiDoc MP Imaging System
• Mini-PROTEAN TGX Stain-Free Precast Gels

Related Categories

• Fluorescent Western Blotting Antibodies
• Imaging Systems
• Semi-Dry and Rapid Blotting Systems
• Stain-Free Western Blotting Workflow

Supporting Documents

• hFAB Rhodamine Housekeeping Antibodies Instruction Manual
• Fluorescent Western Blotting with StarBright Blue 700 Secondary Antibodies Selection Guide
• StarBright Blue 700 Secondary Antibodies Flier
• Western Blot Normalization Quick Start Guide

Online Resources

• The Fluorescent Way: Why Start Fluorescent Western Blotting Now?
• Fluorescent Western Blotting: Helpful Tips for Optimizing Your Protocol