hFAB™ Rhodamine Housekeeping Protein Fluorescent Primary Antibodies

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Overview

One-step fluorescence detection of housekeeping proteins on western blots, without the need for secondary antibodies. HuCAL® monoclonal antibodies provide exceptional specificity for actin, tubulin, or GAPDH.
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Description

Description

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hFAB Rhodamine Housekeeping Protein Fluorescent Primary Antibodies allow one-step detection of common housekeeping proteins like actin, tubulin, and GAPDH in your samples. These anti-actin, anti-tubulin, and anti-GAPDH antibodies are directly labeled with a rhodamine derivative (Exmax/Emmax = 530 nm/580 nm). The hFAB Rhodamine Antibodies were generated with Human Combinatorial Antibody Library (HuCAL) Technology, providing exceptional specificity.

These housekeeping protein antibodies can be used for protein normalization when the protein loading amounts are in the linear dynamic range. hFAB Rhodamine Antibodies can be imaged with the ChemiDoc MP Imaging System, and can be used in multiplex experiments with StarBright™ Blue 700 Secondary Antibodies as well as other traditional fluorescent antibodies, permitting detection of multiple target proteins and the housekeeping protein in a single blot.

Features and Benefits

  • Simple — No requirement for a secondary antibody; one-step detection of housekeeping proteins using highly cross-adsorbed antibodies for high specificity and sensitivity
  • Versatile — Compatible with use of a wide range of other fluorophores including StarBright Blue 700 and DyLight 800 Fluorophores for multiplex western blotting
  • Unique — HuCAL Technology eliminates cross-reactivity with primary/secondary antibodies from other species
  • Diverse — Antibodies have been tested to detect human or mouse housekeeping proteins

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hucal-hkp-actin-40ul-12004164-tn.jpg
12004164
Pkg of 1, lyophilized rhodamine-conjugated anti-actin human Fab fragment; reconstitute to 40 µl
hucal-hkp-actin-200ul-12004163-tn.jpg
12004163
Pkg of 1, lyophilized rhodamine-conjugated anti-actin human Fab fragment; reconstitute to 200 µl
hucal-hkp-tubulin-40ul-12004166-tn.jpg
12004166
Pkg of 1, lyophilized rhodamine-conjugated anti-tubulin human Fab fragment; reconstitute to 40 µl
hucal-hkp-tubulin-200ul-12004165-tn.jpg
12004165
Pkg of 1, lyophilized rhodamine-conjugated anti-tubulin human Fab fragment; reconstitute to 200 µl
hucal-hkp-gapdh-40ul-12004168-tn.jpg
12004168
Pkg of 1, lyophilized rhodamine-conjugated anti-GAPDH human Fab fragment; reconstitute to 40 µl
hucal-hkp-gapdh-200ul-12004167-tn.jpg
12004167
Pkg of 1, lyophilized rhodamine-conjugated anti-GAPDH human Fab fragment; reconstitute to 200 µl

Documents

Documents

Number Description Options
10000068702
hFAB™ Rhodamine Housekeeping Antibodies Instruction Manual, Ver B
10000078050
Comprehensive Guide to Fluorescent Western Blotting with StarBright and hFAB™ Rhodamine Antibodies
10000068973
Fluorescent Western Blotting with StarBright™ Blue 700 Secondary Antibodies Selection Guide, Ver B
6911
Fluorescent Western Blotting Antibodies Flier