Description
Description
hFAB Rhodamine Housekeeping Protein Fluorescent Primary Antibodies allow one-step detection of common housekeeping proteins like actin, tubulin, and GAPDH in your samples. These anti-actin, anti-tubulin, and anti-GAPDH antibodies are directly labeled with a rhodamine derivative (Exmax/Emmax = 530 nm/580 nm). The hFAB Rhodamine Antibodies were generated with Human Combinatorial Antibody Library (HuCAL) Technology, providing exceptional specificity.
These housekeeping protein antibodies can be used for protein normalization when the protein loading amounts are in the linear dynamic range. hFAB Rhodamine Antibodies can be imaged with the ChemiDoc MP Imaging System, and can be used in multiplex experiments with StarBright™ Blue 700 Secondary Antibodies as well as other traditional fluorescent antibodies, permitting detection of multiple target proteins and the housekeeping protein in a single blot.
Features and Benefits
- Simple — No requirement for a secondary antibody; one-step detection of housekeeping proteins using highly cross-adsorbed antibodies for high specificity and sensitivity
- Versatile — Compatible with use of a wide range of other fluorophores including StarBright Blue 700 and DyLight 800 Fluorophores for multiplex western blotting
- Unique — HuCAL Technology eliminates cross-reactivity with primary/secondary antibodies from other species
- Diverse — Antibodies have been tested to detect human or mouse housekeeping proteins
Product Options
Products |
Cross Reactivity |
Antigen |
Catalog Number |
hFAB Rhodamine Anti-Actin Primary Antibody, 40 µl |
Human, Mouse, and Rat | Recombinant human β-actin expressed in E. coli | |
hFAB Rhodamine Anti-Actin Primary Antibody, 200 µl |
Human, Mouse, and Rat | Recombinant human β-actin expressed in E. coli | |
hFAB Rhodamine Anti-Tubulin Primary Antibody, 40 µl |
Human, Mouse, and Rat | Recombinant human β-tubulin expressed in E. coli | |
hFAB Rhodamine Anti-Tubulin Primary Antibody, 200 µl |
Human, Mouse, and Rat | Recombinant human β-tubulin expressed in E. coli | |
hFAB Rhodamine Anti-GAPDH Primary Antibody, 40 µl |
Human, Mouse, and Rat | Recombinant human GAPDH expressed in E. coli | |
hFAB Rhodamine Anti-GAPDH Primary Antibody, 200 µl |
Human, Mouse, and Rat | Recombinant human GAPDH expressed in E. coli |
Related Products
- Primary Antibodies
- Secondary Antibodies
- ChemiDoc MP Imaging System
- Mini-PROTEAN TGX Stain-Free Precast Gels
Related Categories
- Fluorescent Western Blotting Antibodies
- Imaging Systems
- Semi-Dry and Rapid Blotting Systems
- Stain-Free Western Blotting Workflow
Supporting Documents
- hFAB Rhodamine Housekeeping Antibodies Instruction Manual
- Fluorescent Western Blotting with StarBright Blue 700 Secondary Antibodies Selection Guide
- StarBright Blue 700 Secondary Antibodies Flier
- Western Blot Normalization Quick Start Guide
Online Resources
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