Teach PCR and real-time quantitative PCR (qPCR) with these hands-on lab activities that engage students with real-world contexts. Students will amplify and analyze DNA from forensic (simulated), human, agricultural, and environmental samples to explore the utility of PCR and qPCR in crime scene investigation, medicine, food analysis, and ecology, among other applications.
PCR Kits
DNA Barcoding Kits
Students incorporate PCR, DNA sequencing, and bioinformatics as they genetically identify species of fish, mammals, insects, or fungi using a DNA barcode.
Crime Scene Investigator PCR Basics Kit
Students use pre-made DNA samples to simulate DNA profiling as commonly used in forensic laboratories.
PV92 PCR Informatics Kit
Students extract DNA from their cheek cells or hair follicles and then use PCR and electrophoresis to fingerprint their own DNA.
GMO Investigator Kit
Students extract DNA from food samples and then apply PCR and DNA electrophoresis to test for the presence of two GMO-associated DNA sequences.
Real-Time PCR Kits
Crime Scene Investigator PCR Basics Real-Time PCR Starter Kit
This activity uses the primers and pre-made DNA samples in the Crime Scene Investigator PCR Basics Kit to teach students the fundamentals of real-time PCR.
GMO Investigator Real-Time PCR Starter Kit
An extension of the GMO Investigator Kit, this activity teaches students the fundamentals of real-time PCR and its application in testing foods for the presence and amount of genetic modification.
Educational Resources
PCR
YouTube PCR Playlist
Use these informational and entertaining videos to bring your lessons on PCR to life.
Animation: Polymerase Chain Reaction
The steps of PCR are best visualized through animation.
A Brief History of PCR (PPT 5.7 MB)
An editable file that you can customize as needed.
Real-Time PCR
Real-Time PCR Basics
(PPT 5.7 MB)An editable presentation that explains how real-time PCR works and how it can be used for quantitative purposes.
Real-Time PCR Applications
(PPT 4.3 MB)An editable presentation that explains how real-time PCR works and several of its real-life applications.
Webinar: Discover Real-Time PCR for the Classroom (1:00 Hour)
Narrated by David Palmer, PhD, this webinar covers the basics of real-time PCR with a unique explanation of the calculation of starting DNA quantities from real-time PCR data.
Equipment
PCR Equipment
T100 Thermal Cycler
The 96-well T100 Thermal Cycler offers an intuitive touch screen and reliable performance in a compact footprint for performing PCR in the classroom.
PTC Tempo Thermal Cycler
Teach PCR amplification using this compact yet powerful thermal cycler that offers the latest in Bio-Rad amplification technology, a choice of reaction blocks, and features that make it easy to use for novices and experts alike.
Real-Time PCR Equipment
CFX Duet Real-Time PCR System
Designed for 2-channel multiplex capability and without stand-alone operation, Wi-Fi connectivity, or BR.io compatibility, the CFX Duet System is the perfect choice for many teaching laboratories.
CFX Opus Real-Time PCR Systems
Available in 96-well (5-color detection) and 384-well (4-color detection) formats for multiplex assays, the CFX Opus Real-Time PCR Systems offer flexible options for stand-alone or remote operation and data retrieval. It represents Bio-Rad’s premium offering for real-time qPCR.
Looking for PCR or real-time PCR products for your research? See Bio-Rad's products for Life Science Research.