GMO Investigator Kit

Overview

Kit contains sufficient materials for 8 student workstations (2–4 students per workstation)
Bio-Rad certified non-GMO food control	1
InstaGene matrix, 20 ml	1
GMO positive control DNA, 500 µl	1
PCR master mix (Taq DNA polymerase, dNTPs, buffers), 1.2 ml	1
GMO primers (red), 15 µl	1
Plant PSII primers (green), 15 µl	1
PCR molecular weight ruler, 200 µl	1
Orange G loading dye, 1 ml	1
Disposable plastic transfer pipets	20
Micro test tubes, 1.5 ml	60
Screwcap tubes, 1.5 ml	50
PCR tubes, 0.2 ml	50
Capless PCR tube adaptors, 1.5 ml	50
Foam micro test tube holders	8
Curriculum, including Teacher's Guide, Student Manual, and graphic Quick Guide	1
Required Accessories Not Included in Kit:
Obtain food samples locally
Horizontal gel electrophoresis chambers	4–8
Adjustable micropipets
2–20 µl	1–8
20–200 µl	1
Pipet tips, aerosol barrier
2–20 µl, Xcluda B	1 box
20–200 µl, Xcluda D	1 box
Water bath	1
Microcentrifuges	1–4
Thermal cycler	1
Power supplies	2–4
Mortars and pestles	1–8
Balance with range 0.5–2 g	1
Weigh boats or paper	16
Recommended (Optional) Accessories:
Rocking platform
Microwave oven

GMO Investigator Flowchart

Polymerase Chain Reaction

Key Kit Features

Extract and amplify DNA from 8 food samples
Perform genuine diagnostic procedures
Use PCR and electrophoresis
Complete in three 45 minute lab sessions

Purchasing Guide Table

Reduce. Reuse. Refresh.

Refresh kit components, reduce packaging waste, reuse components, and refresh your kits, and you’ll save storage space by purchasing individual items. Bio-Rad now has many individual components for Biotechnology Explorer™ kits available for purchase.

To read more click here.

Curriculum Fit

Conventional PCR Animation

GM Debate

Are GM crops a good thing? Many people who object to the use of GM crops argue that there is a potential for "superweeds" to arise through cross-pollination with herbicide resistant crops, or that "superbugs" will evolve to be resistant to the toxins in pest-resistant crops. Many are concerned about potential allergic reactions to novel proteins, antibiotic resistance arising from the selectable markers used to develop the crops, or other unforeseen effects on public health. Some voice concerns that we have not done enough research to fully understand the implications of altering the planet's plant diversity.

Proponents of GM crops and foods argue that these crops are beneficial for the environment because they reduce the use of herbicides and pesticides that are toxic to the environment and human health. In addition, GM crops may preserve arable land by reducing stresses on the land, improve the nutritional value of food for developing countries, and allow crops to be grown on previously nonarable land.

Regardless of where your students stand on the GM debate, won't they be interested to know how much of the corn- or soy-based foods they eat have been genetically modified?

Large Class Preparation Guide

Learn tips and techniques for preparing agar plates and agarose gels in large quantities.

To read more click here

Complete Purchasing Guide

Download the complete Biotechnology Explorer™ Refresh Kit Components Purchasing Guide.

To read more click here.

Use the interactive table to calculate how many kits and refresh kit components to order.

PowerPoint Presentation for Educational Use

PowerPoint Presentation for Workshops and for Teaching

GMO Investigator Kit

This PowerPoint presentation is intended for educational use only. The presentation provides educators with background on the GMO Investigator Kit and includes instructions for using the kit.

Download PowerPoint presentation

This kit uses PCR and DNA electrophoresis to test for the presence of two different GMO-associated DNA sequences: the 35S promoter of CaMV and the terminator of the nos gene of A. tumefaciens.
Read full description

Ordering Information

Description
Specifications
Ordering
Refills
Documents

Description

The GMO Investigator Kit uses PCR and DNA electrophoresis to test for the presence of two different GMO-associated DNA sequences: the 35S promoter of the cauliflower mosaic virus and the terminator of the nopaline synthase gene of Agrobacterium tumefaciens. These DNA sequences are present in >85% of the GM crops that are approved for distribution worldwide. As a control for the integrity of the plant DNA extracted from food, PCR is used to amplify a section of the photosystem II chloroplast gene that is common to most higher plants. Students engage in a complete investigation in which they gather sample food items from the grocery store, extract DNA from the samples, amplify the DNA using polymerase chain reaction (PCR), and use agarose gel electrophoresis to identify the presence or absence of amplified GMO sequences.

Features and Benefits

The kit allows a guided-inquiry approach. Students conduct sophisticated scientific procedures employing multiple levels of controls that allow them to assess the validity of their results. Students are able to:

Extract and amplify DNA from eight food samples
Perform genuine diagnostic procedures
Use PCR and electrophoresis

Students determine the presence or absence of GMO sequences in their food samples and answer the following questions:

Did we successfully extract DNA?
Did our PCR work as expected?
Do we have GM content?

Regardless of where your students stand on the GM debate, won't they be interested to know how much of the corn– or soy–based foods they eat has been genetically modified?

More Information

Three-session laboratory activity, 45 min per session
Provides sufficient materials for eight student workstations, up to four students per workstation

Specifications

Kit contains sufficient materials for 8 student workstations (2–4 students per workstation)
Bio-Rad certified non-GMO food control	1
InstaGene™ Matrix, 20 ml	1
GMO positive control DNA, 500 µl	1
PCR master mix (Taq DNA polymerase, dNTPs, buffers), 1.2 ml	1
GMO primers (red), 15 µl	1
Plant PSll primers (green), 15 µl	1
PCR molecular weight ruler, 200 µl	1
Orange G loading dye, 1 ml	1
Disposable plastic transfer pipets	20
Micro test tubes, 1.5 ml	60
Screwcap tubes, 1.5 ml	50
PCR tubes, 0.2 ml	50
Capless PCR tube adaptors, 1.5 ml	50
Foam micro test holders	8
Curriculum, including Teacher's Guide, Student Manual, and graphic Quick Guide	1

Ordering

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GMO Investigator Kit

1662500EDU
Gel electrophoresis classroom study kit, includes positive control, InstaGene matrix, master mix, primers, dye, test tubes/holders, curriculum, and more, for 32 students; education use only

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GMO Investigator Kit Plus Small DNA Electrophoresis Reagent Pack

1662550EDU
Classroom study kit includes GMO Investigator kit (1662500EDU) and reagents sufficient to pour, run, and stain forty-eight 1% or sixteen 3% 7 x 10 cm agarose gels; education use only

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Refills

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GMO Investigator Kit Reagent Refill Pack

1662501EDU
Consumables refill kit for use with GMO Investigator Kit (1662500EDU), contains PCR master mix, primers, positive control, PCR molecular weight ruler, Orange G loading dye; education use only

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InstaGene Matrix

7326030EDU
20 ml, Chelex-based resin for PCR-ready DNA purification, 100 extractions; education use only

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50x Tris/Acetic Acid/EDTA (TAE)

1660742EDU
100 ml, TAE nucleic acid electrophoresis buffer solution, pH 8.0; education use only

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Master Mix For PCR

1665009EDU
90 units, 1.2 ml includes 0.075 units per µl Taq DNA polymerase, reaction buffer, 4 mM MgCl₂, 0.4 mM of each dNTP (dATP, dCTP, dGTP, dTTP); education use only

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Jellyfish Foam Floating Racks

1660479EDU
Pkg of 8, microcentrifuge tube rack, holds 12 tubes; education use only

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Gel Staining Trays

1660477EDU
Pkg of 4; education use only

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Disposable Plastic Transfer Pipets

1660474EDU
Pkg of 500, sterile; education use only

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Documents

Number	Description	Options
5396	Fast Gel Protocol — Easily Run Your DNA Gels in Under 10 Minutes — Bio-Rad Explorer, Ver D	Click to download
5290	GMO Investigator Kit, Biotechnology Explorer, Quick Guide	Click to download
10002155	Instruction Manual, GMO Investigator Kit, Biotechnology Explorer, Rev C	Please login to download this document
5891	Refresh Kit Components — Large Class/Multiple Class Preparation Guide	Click to download
5872	Bio-Rad Explorer™ Refresh Kit Components Purchasing Guide	Click to download
6602	UView™ 6x Loading Dye and Stain Guide, Bio-Rad Explorer™	Click to download
7151	Science, Food, and Environment AP Strand Poster, Ver A	Click to download

This product contains electrical or electronic material. Learn more about Bio-Rad's EU Recycle Program

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Call us at 1-800-4-BIORAD (1-800-424-6723)

Where can I find the catalog number, SKU number, or product number?

The catalog number, SKU number, or product number is printed on your product label. See the sample label below for the location of this information.

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I have a batch number, not a lot/control number. How do I find the Certificate of Analysis for my item?

You can use the batch number in place of the lot/control number. Use the Certificate of Analysis search tool, entering the catalog number as usual and the batch number where the lot/control number would go.

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GMO Investigator Kit

Overview

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GMO Investigator Kit

Description

Specifications

Ordering

GMO Investigator Kit

GMO Investigator Kit Plus Small DNA Electrophoresis Reagent Pack

Refills

GMO Investigator Kit Reagent Refill Pack

InstaGene Matrix

50x Tris/Acetic Acid/EDTA (TAE)

Master Mix For PCR

Jellyfish Foam Floating Racks

Gel Staining Trays

Disposable Plastic Transfer Pipets

Documents

Where can I find the catalog number, SKU number, or product number?

Where can I find the lot or control number?

Why are Certificates of Analysis not on the Documents tab?

I have a batch number, not a lot/control number. How do I find the Certificate of Analysis for my item?

Can I get a Certificate of Analysis even if my item is expired?

Why does it say "Product Name Not Found" for a Certificate of Analysis listed in the search results?

Why does it say "Not Available" for a Certificate of Analysis listed in the search results?

Support

Documents

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