CFX Duet Real-Time PCR System for the Classroom

CFX Duet Real-Time PCR System for the Classroom

Overview

Teach real-time quantitative PCR (qPCR) with this industry-quality but economical system designed for singleplex and duplex experiments.

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Ordering Information

Description

Description

Contact a Specialist

Note: This page provides ordering information for educational uses of the CFX Duet Real-Time PCR System. To order for research applications, please visit the Life Science Research page for the CFX Duet System.

 

Teach real-time quantitative PCR (qPCR) in your classroom laboratory with the compact, cost-effective CFX Duet Real-Time PCR System.

The CFX Duet System brings Bio-Rad qPCR technology to the hands of your students by offering the same performance characteristics as the CFX Opus platform but at a lower price point. Designed for 2-channel multiplex capability and without stand-alone operation, Wi-Fi connectivity, or BR.io compatibility, the CFX Duet System is the perfect choice for many teaching laboratories.

  • Quick system set up — easy installation and factory-calibrated optics
  • Our best thermal uniformity and accuracy to date
  • Uniform optical system free of regular calibration requirements
  • No need for a reference/normalizing dye
  • Can perform duplex assays, including genotyping
  • Connects to a PC running CFX Maestro Software (version 2.3 or higher, sold separately)

CFX Maestro Software — Real-Time PCR Data Collection, Analysis, and Statistics

Operation of the CFX Duet System requires connection to a PC running CFX Maestro Software (sold separately). This software tool helps you set up your qPCR experiments, select reference genes, perform statistical analyses, and graph your results.

Teach Real-Time PCR (qPCR) Using Bio-Rad Explorer Classroom Kits

Bio-Rad offers educational kits that help you teach the principles and techniques of qPCR and how it compares with traditional PCR:

Other Resources

Specifications

Specifications

Thermal Cycler
Maximum ramp rate, °C/sec
5
Average ramp rate, °C/sec
3.3
Lid, °C
30–110
Temperature
Accuracy, °C
±0.2 of programmed target at 90°C
Uniformity, °C
±0.3 well-to-well after 10 sec of arrival at target
Gradient
Operational range, °C
30–100
Programmable span, °C
1–24
Optical Detection
Excitation
2 filtered LEDs
Detection
3 filtered photodiodes
Range of excitation/emission wavelengths, nm
450–580
Sensitivity
Detects 1 copy of target sequence in human genomic DNA
Dynamic range
10 orders of magnitude
Scan Time
All channels, sec
12
FAM/SYBR® Green only, sec
3
Software
Operating system
Windows 10 and up
Multiplex analysis
Up to 2 targets per well
System
Sample capacity, wells
96
Sample size, µl
1–50 (10–50 recommended)
Communication interface
USB 2.0
Electrical approvals
IEC, CE
Dimensions (W x D x H), cm/in
33x56x36 / 13x22x14
Weight, kg/lb
22/48

Ordering

Ordering

items

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CFX Duet Real-Time PCR System
(Discontinued) CFX Duet Real-Time PCR Instrument
12016265EDU

96-well, 2-color plus FRET, real-time PCR detection system, includes CFX Duet base unit, cables; for educational use only (for research instrument, see 12016265)

cfx-maestro-software-12004110-tn.jpg
(Discontinued) CFX Maestro 2.3 Software for Windows PC
12013758EDU

1 license, CFX Maestro 2.3, software for real-time PCR plate setup, data collection, statistics, and graphing of results; for Windows PCs; for educational use only

Kits & Reagents

Kits & Reagents

170-8880EDU_tn.jpg
(Discontinued) iQ SYBR® Green Supermix
1708880EDU
2.5 ml (2 x 1.25 ml), 2x qPCR mix, contains dNTPs, iTaq™ DNA polymerase, MgCl2, SYBR® Green I, enhancers, stabilizers, fluorescein, for 100 x 50 µl reactions; education use only
ssoadvanced-universal-sybr-green-supermix-172-5270-thumb.jpg
(Discontinued) SsoAdvanced Universal SYBR Green Supermix, 200 x 20 µl rxns, 2 ml (2 x 1 ml)
1725270EDU
2 ml (2 x 1 ml vials), 2x qPCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, SYBR Green I, ROX normalization dyes, for 200 x 20 µl reactions; for educational use only
1725271EDU-sku-tn
(Discontinued) SsoAdvanced Universal SYBR Green Supermix, 500 x 20 µl rxns, 5 ml (5 x 1 ml)
1725271EDU

5 ml SsoAdvanced Universal SYBR Green Supermix (5 x 1 ml vials), 2x qPCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, SYBR Green I, ROX normalization dyes, for 500 x 20 µl reactions; for educational use only 

HSP9601_tn.jpg
(Discontinued) Hard-Shell 96-Well PCR Plates, low profile, thin wall, skirted, white/clear
HSP9601EDU

Pkg of 50, white shell/clear well PCR plate, rigid 2-component design; for educational use only (see HSP9601 for research use)

(Discontinued) Microseal 'B' Adhesive Seals
MSB1001EDU
Pkg of 100, optically clear seal for PCR plates used in real-time and conventional PCR reactions; education use only

Educational Kits

Educational Kits

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(Discontinued) Crime Scene Investigator PCR Basics Real-Time PCR Starter Kit
1662660EDU
Introductory real-time PCR classroom study kit, contains Crime Scene Investigator PCR Basics kit (1662600EDU) and additional real-time reagents; education use only
166-2560EDU_tn.jpg
(Discontinued) GMO Real-Time PCR Starter Kit
1662560EDU

Introductory real-time PCR classroom study kit, contains GMO Investigator Kit (1662500EDU) and additional real-time reagents; education use only

Documents

Documents

Number Description Options
10000148048
CFX Duet Real-Time PCR System Instrument Guide
10000147782
CFX Duet Real-Time PCR System Quick Start Guide
3332
Solutions for Teaching Real-Time PCR