GMO Investigator Real-Time PCR Starter Kit

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Overview

The GMO Investigator real-time PCR starter kit is designed for teaching students the principles of PCR and its use in testing foods for the presence of genetic modifications.

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Description

Description

DNA gels

Run your DNA gels in 10 minutes!

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Polymerase chain reaction (PCR) is now such a fundamental technique in the biotechnology laboratory that L. A. Pray wrote in 2004: "PCR is to biology what petroleum is to transportation." PCR is a basis for multiple ways — ranging from DNA fingerprinting and sequencing to mutagenesis — to analyze and detect nucleic acids. Real-time PCR is an extremely valuable analytical tool that not only reveals what DNA is present but how much. Real-time PCR is becoming the most widely used PCR application for genomic and gene expression analysis in research laboratories; it also is rapidly establishing itself as a technique in the clinical diagnostic lab. The need for faster, more accurate, and more economical systems with a high throughput has fueled the popularity of real-time PCR.

Using genomic DNA as the template for amplification, real-time PCR can be used in infectious disease diagnostics to rapidly determine levels of specific pathogens in various tissues. The molecular diagnostic laboratory also relies heavily on real-time PCR for detecting aneuploidies and diagnosising other genetic diseases. In microbiology laboratories, real-time PCR can be used to detect and quantitate various microbial contaminants in environmental samples.

The GMO Investigator real-time PCR starter kit is designed for teaching students the principles of PCR and its use in testing foods for the presence of genetic modifications. This kit can be used to quantitate the amount of DNA in a plant and to compare it with the level of genetically modified DNA that is recovered from each food sample. It is even possible to determine what fraction of a food product is made with genetically modified ingredients, in the same manner as standard testing laboratories do.

Reference

Pray LA (2004). Consider the Cycler, The Scientist 18, 34–37.

 

 

 

Specifications

Specifications

Kit contains sufficient materials for 8 student workstations (2–4 students per workstation), plus additional real-time reagents for further studies
 
GMO Investigator kit
1
iQ™ SYBR® Green Supermix, 2.5 ml
1
Agarose powder, 25 g
1
Electrophoresis buffer, 50x TAE, 100 ml
1
Fast Blast™ DNA stain, 500x, 100 ml
1
Sterile water, 500 ml
1
PCR tube strips
120
Optical flat caps
120
Curriculum, including Teacher's Guide and Student Manual
1

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1662560EDU

Introductory real-time PCR classroom study kit, contains GMO Investigator Kit (1662500EDU) and additional real-time reagents; education use only

Accessories

Accessories

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1709799EDU
Applications guidebook providing considerations for designing, analyzing, and optimizing real-time PCR experiments; education use only

Refills

Refills

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(Discontinued) GMO Investigator Kit
1662500EDU
Gel electrophoresis classroom study kit, includes positive control, InstaGene matrix, master mix, primers, dye, test tubes/holders, curriculum, and more, for 32 students; education use only
166-2501EDU_tn.jpg
1662501EDU

Consumables refill kit for use with GMO Investigator Kit (1662500EDU), contains PCR master mix, primers, positive control, PCR molecular weight ruler, Orange G loading dye; education use only

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(Discontinued) Master Mix For PCR
1665009EDU
90 units, 1.2 ml includes 0.075 units per µl Taq DNA polymerase, reaction buffer, 4 mM MgCl2, 0.4 mM of each dNTP (dATP, dCTP, dGTP, dTTP); education use only
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1708880EDU
2.5 ml (2 x 1.25 ml), 2x qPCR mix, contains dNTPs, iTaq™ DNA polymerase, MgCl2, SYBR® Green I, enhancers, stabilizers, fluorescein, for 100 x 50 µl reactions; education use only
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TLS0851EDU
Pkg of 120, thin-wall polypropylene 8-tube strips for PCR and low volume reactions, white, (960 tubes); education use only
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MLL4851EDU
Pkg of 50, white PCR plate, 8 x 6 format, one-component polypropylene, can be cut for fewer samples; education use only

Documents

Documents

Number Description Options
10002155
Instruction Manual, GMO Investigator Kit, Biotechnology Explorer, Rev C
M1662505
GMO Investigator Kit: A Quantitative Real-Time PCR Extension, Biotechnology Explorer, Application Note, Rev A
5396
Fast Gel Protocol — Easily Run Your DNA Gels in Under 10 Minutes — Bio-Rad Explorer, Ver D