This unique, modular, and multiweek laboratory course that guides students through a molecular biology workflow is identical to that performed in a working science lab. Students clone, sequence, and analyze a housekeeping gene in the GAPDH family, taken from a plant of their choice. The final sequencing and bioinformatics module allows each class to produce novel data - the real thing!
The full Cloning and Sequencing Explorer Series can be purchased as a bundle or as individual modules. See the individual products below for more details
- Cloning and Sequencing Explorer Series
The complete Cloning and Sequencing Explorer series is a 6–8 week series of laboratory activities in which students clone and analyze a portion of the GAPC gene. Activities range from DNA extraction to computer-based sequence analysis.
- Nucleic Acid Extraction Module
The first step in a gene cloning lab, the Nucleic Acid Extraction module allows students to extract all nucleic acids from plant cells using tissue grinding and lysis, followed by purification using column chromatography.
- GAPDH PCR Module
An advanced PCR lab that utilizes nested PCR, the GAPDH PCR module allows students to amplify a portion of the GAPC gene, a housekeeping gene in the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) family, from a plant of their choice.
- Electrophoresis Module
These DNA electrophoresis reagent packs are available in small, medium, and large sizes. Students can pour their own gels to visualize the products of PCR reactions, ligations, and restriction digests or perform dye electrophoresis.
- Ligation and Transformation Module
This module allows students to directly ligate purified PCR products into the pJET1.2 plasmid vector and immediately transform freshly prepared competent bacteria with the ligation reaction.
- Microbial Culturing Module
The Microbial Culturing module contains the materials necessary to teach basic sterile technique, culture and transform ampicillin-resistant E. coli, and grow cultures for plasmid minipreps.
- Aurum™ Plasmid Mini Purification Module
In this module, students can rapidly purify up to 20 µg of plasmid DNA from transformed bacteria using a three-step alkaline lysis procedure followed by mini column chromatography.
- Sequencing and Bioinformatics Module
The sequencing and bioinformatics module is used to analyze a portion of the GAPC gene isolated from a plant. Students are guided through a sequence analysis that allows them to make a real contribution to science!