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Overview
Prepacked Spin Columns
| Prepacked Spin Column Selection Guide | |||||
| Bio-Spin 6 | Micro Bio-Spin 6 | Bio-Spin 30 | Micro Bio-Spin 30 | PCR Kleen | |
| Packed support | Special grade Bio-Gel P-6 gel | Special grade Bio-Gel P-6 gel | Special grade Bio-Gel P-30 gel | Special grade Bio-Gel P-30 gel | Special grade size exclusion gel |
| Equilibration buffer | 10 mM Tris, pH 7.4 or SSC buffer* | 10 mM Tris, pH 7.4 or SSC buffer* | 10 mM Tris, pH 7.4 or SSC buffer* | 10 mM Tris, pH 7.4 or SSC buffer* | 10 mM Tris, 1 mM EDTA, pH 7.0 |
| Applications Desalting of oligonudeotides >20 bases | • | • | — | — | — |
| Labeling reactions: removal of unincorporated nucleotides >20 bases or bp from DNA | — | — | • | • | — |
| Removal of primers and primer-dimers from PCR products >200 bp | — | — | — | — | • |
| Buffer exchange (restriction fragments, PCR products, enzyme reactions, sequencing templates) | • | • | — | • | — |
| DNA sequencing reaction mixture cleanup** | — | — | • | • | — |
| Riboprobe cleanup*** | — | — | — | • | — |
| Desalting of antibody, enzyme, and protein solutions | • | • | — | • | — |
| Purification of proteins of molecular weight >6, 000 | • | • | — | — | — |
| Purification of proteins of molecular weight >40,000 | — | — | • | • | — |
| Bed volume | 1.1 ml | 0.7 ml | 1.1 ml | 0.7 ml | 0.6 ml |
| Retention and recovery | 90% recovery of 20 bases or bp, 99% retention of salts | 90% recovery of 20 bases or bp, 99% retention of salts | 95% recovery of 22 bases or bp, 98% retention of ddNTPs | 95% recovery of 22 bases or bp, 98% retention of ddNTPs | 85% recovery of ≥700 bp, 95% retention of primers and primer-dimers |
| Molecular weight exclusion limit, globular proteins | 6,000 | 6,000 | 40,000 | 40,000 | 8,000,000 |
| Sample volume | 50–100 µl | 10–75 µl | 50–100 µl | 10–75 µl | 25–100 µl |
| Centrifuge type | Swinging bucket | Microcentrifuge | Swinging bucket | Microcentrifuge | Microcentrifuge |
| Autoclavability | Yes | Yes | Yes | Yes | Yes |
* 150 mM NaCl, 17.5 mM sodium citrate, pH 7.0.
** In Tris buffer.
*** In RNase-free Tris buffer.
Rapid Removal of Primer-dimers
Effective, rapid removal of primer-dimers from PCR reactions. Two PCR reaction mixtures were purified with PCR Kleen spin columns. Lane 1, a mixture of 2 µg of a 660 bp DNA fragment with 100 ng of a 25-mer primer-dimer; lane 3, a mixture of 2 µg of a 660 bp DNA fragment with 500 ng of a 25-mer primer; lane 2, primer-dimer purification; lane 4, primer purification; M, DNA markers. Reaction products and purified samples (10 µl each) were analyzed on a 1.5% agarose gel.
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I cannot find the Certificate of Analysis I am looking for. Help!
When using our online Certificate of Analysis search tool, keep the following in mind:
- Make sure you entered the correct catalog number and lot/control number in the search fields
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Where can I find the catalog number, SKU number, or product number?
The catalog number, SKU number, or product number is printed on your product label. See the sample label below for the location of this information.
Where can I find the lot or control number?
The lot number or control number (one or the other, not both) is printed on your product label. See the sample label below for the location of this information.
Why are Certificates of Analysis not on the Documents tab?
Certificates of Analysis are associated not only with a product, but also with a specific lot of that product. For a given product, there may be several Certificates of Analysis, especially if it is an older product line and has had several lots produced over the years. Using the Certificate of Analysis search tool, you can enter the catalog number and lot/control number for the specific product you have on hand and download the exact Certificate of Analysis you need.
I have a batch number, not a lot/control number. How do I find the Certificate of Analysis for my item?
You can use the batch number in place of the lot/control number. Use the Certificate of Analysis search tool, entering the catalog number as usual and the batch number where the lot/control number would go.
Can I get a Certificate of Analysis even if my item is expired?
Yes. While we do periodically remove Certificates of Analysis for site maintenance purposes, we typically keep them available well beyond a product's expiration date.
Why does it say "Product Name Not Found" for a Certificate of Analysis listed in the search results?
There are Certificates of Analysis that correspond to products that are either discontinued or simply not available on the website. In these situations, the Certificate of Analysis is available for download, but other product details, such as the product name, are not.
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Please use the Request Form here or contact your local Bio-Rad Representative so that we can send you the Certificate of Analysis.
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