Cloning and Sequencing Explorer Series

Overview

Flowchart

Lab Preparation Checklist

Planning Guide

Conventional PCR Animation

The complete Cloning and Sequencing Explorer series is a 6–8 week series of laboratory activities in which students clone and analyze a portion of the GAPC gene. Activities range from DNA extraction to computer-based sequence analysis.

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Description

The Cloning and Sequencing Explorer Series gives your students the tools to perform novel research and contribute to the world's body of scientific knowledge. Over 8 modules, students use classic and cutting-edge molecular biology techniques to clone and sequence a portion of the GAPC gene from a plant of their choice. GAPC is a housekeeping gene in the glyeraldehyde-3-phosphate dehydrogenase (GAPDH) family and is well conserved among plants.

The Cloning and Sequencing Explorer Series guides students through the following experimental workflow steps:

Extract genomic DNA from plants
Amplify a GAPDH gene using PCR and analyze using electrophoresis
Purify the PCR product
Ligate the PCR product into a plasmid
Transform the ligated plasmid into bacteria
Grow minipreps of the transformed bacteria
Isolate plasmid DNA and analyze by restriction digestion
Sequence plasmid DNA (sequencing service not included)
Analyze GAPDH gene sequence using bioinformatics

Features and Benefits

Designed by undergraduate and community college educators, this course was specifically developed to engage your students in authentic research, while ensuring success for both you and them. Tailored experimental controls allow your students to:

Continue the experiment to its conclusion even with negative results
Make mistakes, learn from them, and still complete every step of the workflow
Experience firsthand the satisfaction of completing a successful research project
Apply molecular biology skills and techniques to complete a real-world research project

The Cloning and Sequencing Explorer Series allows students to apply molecular biology skills and techniques to complete a real-world research project, potentially encouraging them to pursue a career in research.

Now with Geneious Prime!

The Cloning and Sequencing Explorer Series now includes a 4-month subscription to Geneious Prime Bioinformatics Software to support your students' scientific discovery.

For more information, visit the Sequencing and Bioinformatics Module product page.

More Information

6–8 week series of laboratory activities made up of eight modular kits
Provides sufficient materials for 12 student workstations, up to four students per workstation
Sequencing service not included.

Additional Resources

Cloning and Sequencing Explorer Series Instruction Manual
Example Chinese Broccoli sequence files

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(Discontinued) Cloning and Sequencing Explorer Series

1665000EDU

Molecular biology study kit, includes 8 modules with lab activities from DNA extraction and cloning to computer-based sequence analysis, curriculum (sequencing service not included); education use only

Refills

(Discontinued) Nucleic Acid Extraction Module

1665005EDU

First module in Cloning and Sequencing Explorer Series classroom study kit, includes buffers, DTT, test tubes

(Discontinued) GAPDH PCR Module

1665010EDU

Second module in Cloning and Sequencing Explorer Series classroom study kit, includes primers, PCR master mix, test tubes/holders, curriculum

(Discontinued) Small Ethidium Bromide DNA Electrophoresis Reagents Pack

1660451EDU

Agarose gel preparation kit,makes 16–48 mini gels, includes agarose powder, ethidium bromide, buffer; education use only

(Discontinued) Small Fast Blast DNA Electrophoresis Reagents Pack

1660450EDU

Agarose gel preparation kit,makes 16–48 mini gels, includes agarose powder, Fast Blast DNA Stain, buffer; education use only

(Discontinued) Medium Fast Blast DNA Electrophoresis Reagent Pack

1660455EDU

Agarose gel preparation kit,makes 90–270 mini gels, includes agarose powder, Fast Blast DNA Stain, buffer; education use only

(Discontinued) Large Fast Blast DNA Electrophoresis Reagent Pack

1660460EDU

Agarose gel preparation kit,makes 360–1,080 mini gels, includes agarose powder, Fast Blast DNA Stain, buffer; education use only

(Discontinued) Medium Ethidium Bromide DNA Electrophoresis Reagents Pack

1660456EDU

Agarose gel preparation kit,makes 90–270 mini gels, includes agarose powder, ethidium bromide, buffer; education use only

(Discontinued) Large Ethidium Bromide DNA Electrophoresis Reagents Pack

1660461EDU

Agarose gel preparation kit,makes 360–1,080 mini gels, includes agarose powder, ethidium bromide, buffer; education use only

(Discontinued) PCR Kleen™ Spin Purification Module

7326300EDU

Fourth module in Cloning and Sequencing Explorer Series classroom study kit, includes PCR Kleen Spin columns, test tubes, curriculum, for 12 workstations; education use only

(Discontinued) Ligation And Transformation Module

1665015EDU

Fifth module in Cloning and Sequencing Explorer Series classroom study kit, includes T4 ligase, cloning vector, growth medium, curriculum, and more, for 12 workstations; education use only

(Discontinued) Microbial Culturing Module

1665020EDU

Sixth module in Cloning and Sequencing Explorer Series classroom study kit, includes ampicillin, petri dishes, E. coli strain HB101 K-12, curriculum, and more, for 12 workstations; education use only

(Discontinued) Aurum Plasmid Mini Purification Kit

7326400EDU

Plasmid purification kit contains plasmid-binding mini columns, capless collection tubes, reagents, for 100 preps

(Discontinued) Sequencing and Bioinformatics Module

1665025EDU

Eighth module in Cloning and Sequencing Explorer Series classroom study kit, includes primers, plasmid, barcoded 96-well plate, test tubes, curriculum, and more, for 12 workstations; education use only (Sequencing service not included)

(Discontinued) Cloning and Sequencing Explorer Series Instruction Manual

1665001EDU

CD-ROM for use with Cloning and Sequencing Explorer series (1665000EDU), includes electronic version of instruction manual (PDF format); education use only

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Cbroccoli_1_GAPSEQ_Forward.ab1

Cbroccoli_1_GAPSEQ_Forward.ab1

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Software Download

EXPORT COMPLIANCE REQUIREMENTS

Export of any Bio-Rad product or technical information relating to the product (i.e., manuals, memoranda, specifications, schematics, etc.) outside of the United States is subject to U.S. export control laws, including but not limited to the U.S. Export-Re-export Requirements and Enhanced Proliferation Control Initiative.

You agree that, unless You receive prior authorization from the United States government, You and/or Your organization shall not:

use any Bio-Rad product if You are located in, under the control of, or a national or resident of any embargoed or restricted* countries (Cuba, Iran, *North Korea, Sudan, and *Syria); or
sell, license, or otherwise provide or ship, directly or indirectly, any Bio-Rad product or technical data (or the direct product thereof) for export or re-export to the embargoed or restricted* countries (Cuba, Iran, *North Korea, Sudan, and *Syria); or
transfer, export or re-export any Bio-Rad product to Your customers or any intermediate entity in the chain of supply if any Bio-Rad product will be used in the design, development, production, stockpiling or use of missiles, chemical or biological weapons or for nuclear end uses; or
transfer, export or re-export, directly or indirectly, any Bio-Rad product, technology or software (or any part thereof, or the product of such technology or software, or any process or service which is the product of such technology or software) to any person or entity identified by the U.S. Department of Commerce as a sanctioned and/or embargoed person or entity. (For a current list of all sanctioned and/or embargoed persons or entities, please consult the U.S. Department of Commerce).

All rights to use the Bio-Rad product(s) are granted on condition that such rights are forfeited if You fail to comply with the terms of these Export Compliance Requirements.

For more information regarding U.S. export laws and regulations, contact the U.S. Department of Commerce and the U.S. Department of the Treasury, Office of Foreign Assets Control.

I agree with the compliance requirements.
(You are required to agree in order to download the software.)

Download

Cbroccoli_1_GAPSEQ_Reverse.ab1

Cbroccoli_1_GAPSEQ_Reverse.ab1

Download Software

Software Download

EXPORT COMPLIANCE REQUIREMENTS

You agree that, unless You receive prior authorization from the United States government, You and/or Your organization shall not:

use any Bio-Rad product if You are located in, under the control of, or a national or resident of any embargoed or restricted* countries (Cuba, Iran, *North Korea, Sudan, and *Syria); or
sell, license, or otherwise provide or ship, directly or indirectly, any Bio-Rad product or technical data (or the direct product thereof) for export or re-export to the embargoed or restricted* countries (Cuba, Iran, *North Korea, Sudan, and *Syria); or
transfer, export or re-export any Bio-Rad product to Your customers or any intermediate entity in the chain of supply if any Bio-Rad product will be used in the design, development, production, stockpiling or use of missiles, chemical or biological weapons or for nuclear end uses; or
transfer, export or re-export, directly or indirectly, any Bio-Rad product, technology or software (or any part thereof, or the product of such technology or software, or any process or service which is the product of such technology or software) to any person or entity identified by the U.S. Department of Commerce as a sanctioned and/or embargoed person or entity. (For a current list of all sanctioned and/or embargoed persons or entities, please consult the U.S. Department of Commerce).

All rights to use the Bio-Rad product(s) are granted on condition that such rights are forfeited if You fail to comply with the terms of these Export Compliance Requirements.

For more information regarding U.S. export laws and regulations, contact the U.S. Department of Commerce and the U.S. Department of the Treasury, Office of Foreign Assets Control.

I agree with the compliance requirements.
(You are required to agree in order to download the software.)

Download

Cbroccoli_1_pJETSEQ_Forward.ab1

Cbroccoli_1_pJETSEQ_Forward.ab1

Download Software

Software Download

EXPORT COMPLIANCE REQUIREMENTS

You agree that, unless You receive prior authorization from the United States government, You and/or Your organization shall not:

use any Bio-Rad product if You are located in, under the control of, or a national or resident of any embargoed or restricted* countries (Cuba, Iran, *North Korea, Sudan, and *Syria); or
sell, license, or otherwise provide or ship, directly or indirectly, any Bio-Rad product or technical data (or the direct product thereof) for export or re-export to the embargoed or restricted* countries (Cuba, Iran, *North Korea, Sudan, and *Syria); or
transfer, export or re-export any Bio-Rad product to Your customers or any intermediate entity in the chain of supply if any Bio-Rad product will be used in the design, development, production, stockpiling or use of missiles, chemical or biological weapons or for nuclear end uses; or
transfer, export or re-export, directly or indirectly, any Bio-Rad product, technology or software (or any part thereof, or the product of such technology or software, or any process or service which is the product of such technology or software) to any person or entity identified by the U.S. Department of Commerce as a sanctioned and/or embargoed person or entity. (For a current list of all sanctioned and/or embargoed persons or entities, please consult the U.S. Department of Commerce).

All rights to use the Bio-Rad product(s) are granted on condition that such rights are forfeited if You fail to comply with the terms of these Export Compliance Requirements.

For more information regarding U.S. export laws and regulations, contact the U.S. Department of Commerce and the U.S. Department of the Treasury, Office of Foreign Assets Control.

I agree with the compliance requirements.
(You are required to agree in order to download the software.)

Download

Cbroccoli_1_pJETSEQ_Reverse.ab1

Cbroccoli_1_pJETSEQ_Reverse.ab1

Download Software

Software Download

EXPORT COMPLIANCE REQUIREMENTS

You agree that, unless You receive prior authorization from the United States government, You and/or Your organization shall not:

use any Bio-Rad product if You are located in, under the control of, or a national or resident of any embargoed or restricted* countries (Cuba, Iran, *North Korea, Sudan, and *Syria); or
sell, license, or otherwise provide or ship, directly or indirectly, any Bio-Rad product or technical data (or the direct product thereof) for export or re-export to the embargoed or restricted* countries (Cuba, Iran, *North Korea, Sudan, and *Syria); or
transfer, export or re-export any Bio-Rad product to Your customers or any intermediate entity in the chain of supply if any Bio-Rad product will be used in the design, development, production, stockpiling or use of missiles, chemical or biological weapons or for nuclear end uses; or
transfer, export or re-export, directly or indirectly, any Bio-Rad product, technology or software (or any part thereof, or the product of such technology or software, or any process or service which is the product of such technology or software) to any person or entity identified by the U.S. Department of Commerce as a sanctioned and/or embargoed person or entity. (For a current list of all sanctioned and/or embargoed persons or entities, please consult the U.S. Department of Commerce).

All rights to use the Bio-Rad product(s) are granted on condition that such rights are forfeited if You fail to comply with the terms of these Export Compliance Requirements.

For more information regarding U.S. export laws and regulations, contact the U.S. Department of Commerce and the U.S. Department of the Treasury, Office of Foreign Assets Control.

I agree with the compliance requirements.
(You are required to agree in order to download the software.)

Download

Documents

Number Description Options

10000069187

Bio-Rad Explorer Cloning and Sequencing Explorer Series

5773

Cloning and Sequencing Explorer Series Planning Guide, Bio-Rad Explorer™ , Ver C

6672

Map of AP-Aligned Bio-Rad Kits with Essential Knowledge

6673

Map of AP-Aligned Bio-Rad Kits with Investigative Laboratories

6602

UView™ 6x Loading Dye and Stain Guide, Bio-Rad Explorer™

Cloning and Sequencing Explorer Series

Overview

Description

Description

Features and Benefits

Now with Geneious Prime!

More Information

Additional Resources

Ordering

Ordering

Refills

Refills

Download

Download

Documents

Documents

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