Cloning and Sequencing Explorer Series

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Overview

The complete Cloning and Sequencing Explorer series is a 6–8 week series of laboratory activities in which students clone and analyze a portion of the GAPC gene. Activities range from DNA extraction to computer-based sequence analysis.

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Description

Description

The Cloning and Sequencing Explorer Series gives your students the tools to perform novel research and contribute to the world's body of scientific knowledge. Over 8 modules, students use classic and cutting-edge molecular biology techniques to clone and sequence a portion of the GAPC gene from a plant of their choice. GAPC is a housekeeping gene in the glyeraldehyde-3-phosphate dehydrogenase (GAPDH) family and is well conserved among plants.

The Cloning and Sequencing Explorer Series guides students through the following experimental workflow steps:

  1. Extract genomic DNA from plants
  2. Amplify a GAPDH gene using PCR and analyze using electrophoresis
  3. Purify the PCR product
  4. Ligate the PCR product into a plasmid
  5. Transform the ligated plasmid into bacteria
  6. Grow minipreps of the transformed bacteria
  7. Isolate plasmid DNA and analyze by restriction digestion
  8. Sequence plasmid DNA (sequencing service not included)
  9. Analyze GAPDH gene sequence using bioinformatics

Features and Benefits

Designed by undergraduate and community college educators, this course was specifically developed to engage your students in authentic research, while ensuring success for both you and them. Tailored experimental controls allow your students to:

  • Continue the experiment to its conclusion even with negative results
  • Make mistakes, learn from them, and still complete every step of the workflow
  • Experience firsthand the satisfaction of completing a successful research project
  • Apply molecular biology skills and techniques to complete a real-world research project

The Cloning and Sequencing Explorer Series allows students to apply molecular biology skills and techniques to complete a real-world research project, potentially encouraging them to pursue a career in research.

Now with Geneious Prime!

geneiousThe Cloning and Sequencing Explorer Series now includes a 4-month subscription to Geneious Prime Bioinformatics Software to support your students' scientific discovery.

For more information, visit the Sequencing and Bioinformatics Module product page.

More Information

  • 6–8 week series of laboratory activities made up of eight modular kits
  • Provides sufficient materials for 12 student workstations, up to four students per workstation
  • Sequencing service not included.

Additional Resources

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items

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1665000EDU
Molecular biology study kit, includes 8 modules with lab activities from DNA extraction and cloning to computer-based sequence analysis, curriculum (sequencing service not included); education use only

Refills

Refills

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1665005EDU
First module in Cloning and Sequencing Explorer Series classroom study kit, includes buffers, DTT, test tubes
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(Discontinued) GAPDH PCR Module
1665010EDU
Second module in Cloning and Sequencing Explorer Series classroom study kit, includes primers, PCR master mix, test tubes/holders, curriculum
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1660451EDU
Agarose gel preparation kit,makes 16–48 mini gels, includes agarose powder, ethidium bromide, buffer; education use only
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1660450EDU
Agarose gel preparation kit,makes 16–48 mini gels, includes agarose powder, Fast Blast DNA Stain, buffer; education use only
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1660455EDU
Agarose gel preparation kit,makes 90–270 mini gels, includes agarose powder, Fast Blast DNA Stain, buffer; education use only
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1660460EDU
Agarose gel preparation kit,makes 360–1,080 mini gels, includes agarose powder, Fast Blast DNA Stain, buffer; education use only
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1660456EDU
Agarose gel preparation kit,makes 90–270 mini gels, includes agarose powder, ethidium bromide, buffer; education use only
166-0461EDU_tn.jpg
1660461EDU
Agarose gel preparation kit,makes 360–1,080 mini gels, includes agarose powder, ethidium bromide, buffer; education use only
732-6300EDU_tn.jpg
7326300EDU
Fourth module in Cloning and Sequencing Explorer Series classroom study kit, includes PCR Kleen Spin columns, test tubes, curriculum, for 12 workstations; education use only
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1665015EDU
Fifth module in Cloning and Sequencing Explorer Series classroom study kit, includes T4 ligase, cloning vector, growth medium, curriculum, and more, for 12 workstations; education use only
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1665020EDU
Sixth module in Cloning and Sequencing Explorer Series classroom study kit, includes ampicillin, petri dishes, E. coli strain HB101 K-12, curriculum, and more, for 12 workstations; education use only
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7326400EDU
Plasmid purification kit contains plasmid-binding mini columns, capless collection tubes, reagents, for 100 preps
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1665025EDU
Eighth module in Cloning and Sequencing Explorer Series classroom study kit, includes primers, plasmid, barcoded 96-well plate, test tubes, curriculum, and more, for 12 workstations; education use only (Sequencing service not included)
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1665001EDU
CD-ROM for use with Cloning and Sequencing Explorer series (1665000EDU), includes electronic version of instruction manual (PDF format); education use only

Documents

Documents

Number Description Options
10000069187
Bio-Rad Explorer Cloning and Sequencing Explorer Series, Ver G
5773
Cloning and Sequencing Explorer Series Planning Guide, Bio-Rad Explorer™ , Ver C
6672
Map of AP-Aligned Bio-Rad Kits with Essential Knowledge
6673
Map of AP-Aligned Bio-Rad Kits with Investigative Laboratories
6602
UView™ 6x Loading Dye and Stain Guide, Bio-Rad Explorer™