GAPDH PCR Module

Image
lsr_gapdh_PCR_module.jpg

Overview

An advanced PCR lab that utilizes nested PCR, the GAPDH PCR module allows students to amplify a portion of the GAPC gene, a housekeeping gene in the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) family, from a plant of their choice.

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Description

Description

The GAPDH PCR module is an integral component of the Cloning and Sequencing Explorer Series. You can either use this module in conjunction with the electrophoresis module to visualize PCR results or purchase the module separately as a shorter stand-alone PCR laboratory activity to demonstrate applications of PCR in real research.

The experimental goal of the GAPDH PCR module is to amplify a portion of the GAPC gene, a housekeeping gene in the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) family, from a plant. After choosing a plant, students will amplify genomic and control plasmid DNA using two rounds of PCR from Arabidopsis (provided). Students may also amplify genomic DNA from two other plants, and additional genomic DNA may be extracted using the nucleic acid extraction module. Using degenerate primer sequences and multiple (nested) rounds of PCR, students will learn that amplifying novel DNA sequences can overcome the problems of the degradation of genetic code.

Students can analyze their PCR products using agarose gel electrophoresis and staining with ethidium bromide to visualize the GAPC gene they have isolated from the plant genome using the electrophoresis module.

Specifications

Specifications

Kit contains sufficient materials for 12 student workstations.
 
Initial GAPDH PCR primers, 50 µl
1
Nested GAPDH PCR primers, 50 µl
1
PCR master mix, 1.2 ml
3
Plasmid control DNA, 1 ml
1
Control Arabidopsis genomic DNA
1
Exonuclease I, 50 µl
1
Molecular weight ruler
1
Orange G loading dye, 1 ml
2
Sterile water, 2.5 ml
1
PCR tubes, 0.2 ml
150
Capless PCR tube adaptors, 1.5 ml
150
Colored micro test tubes, 2.0 ml
120
Foam micro test tube holders
12
Curriculum, including teacher's guide, student manual, and graphic quick guide
1

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items

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166-5010EDU_tn.jpg
(Discontinued) GAPDH PCR Module
1665010EDU
Second module in Cloning and Sequencing Explorer Series classroom study kit, includes primers, PCR master mix, test tubes/holders, curriculum

Refills

Refills

166-5011EDU_tn.jpg
1665011EDU
Consumables refill kit for use with GAPDH module (#166-5010EDU), contains primers, PCR master mix, controls, molecular weight ruler, and more; education use only

Documents

Documents

Number Description Options
1665012
Instruction Manual, GAPDH PCR Module, Biotechnology Explorer, Rev A