Aurum™ Plasmid Mini Purification Module

Print
Aurum Plasmid Mini Purification Module

Overview

Lab Preparation Checklist
Kit contains sufficient reagents to purify DNA from 100 minicultures of plasmid-bearing bacteria.
DNA-binding mini columns 100
Capless collection tubes 100
Resuspension solution, 25 ml 1
Lysis solution, 25 ml 1
Neutralization solution, 40 ml 1
5x wash solution, 25 ml 1
Elution solution, 16 ml 1
Instruction manual 1
Required Accessories Not Included in Kit:
95–100% lab grade ethanol  
Microcentrifuge capable of greater than 12,000 x g 1
Shaking water bath 1
Adjustable micropipets
   20–200 µl 1–12
   100–1000 µl 1–12
Pipet tips  
Recommended (Optional) Accessories:
Aurum vacuum manifold

DNA yield >20 µg
Preparation time 10 min
A200/A280 1.75–1.85
Bacterial density Up to OD 12
Purity >99% accuracy*
Purification format Vacuum and spin
Aurum Plasmid Mini Purification Module
CS Module Honeycomb

Isolate plasmid DNA from transformed bacteria. Students inoculate minipreps with transformed bacterial colonies from their agar plates. They then isolate plasmid DNA using a three step alkaline lysis procedure followed by mini column chromatography. Purification can be carried out in microcentrifuges or using the Aurum vacuum manifold.

Once plasmid DNA is eluted, students verify insertion of the PCR product into the plasmid vector using restriction enzyme digestion analysis and agarose gel electrophoresis. Bgl II restriction enzyme, specific for screening the pJET1.2 plasmid, is supplied in the Ligation and Transformation module. Students can also determine plasmid DNA concentration using spectrophotometry or fluorometry in an optional step.

Scroll upScroll down




Purify up to 20 µg of plasmid DNA rapidly and inexpensively without the use of toxic reagents or alcohol precipitations. Plasmid DNA is purified from transformed bacteria using a three-step alkaline lysis procedure followed by mini column chromatography. The highly purified plasmid DNA is free of salts, bacterial chromosomal DNA, and RNA, and is ideal for subsequent molecular biology–based applications such as automated sequencing, cloning, PCR, or restriction digestion analysis.

The Aurum Plasmid Mini Purification Module is an integral component of the Cloning and Sequencing Explorer Series. The module may also be purchased separately and used to isolate plasmid from bacteria containing any cloned gene.

Kit contains sufficient reagents to purify
DNA from 100 minicultures of plasmid-bearing
bacteria.
 
DNA-binding mini columns 100
Capless collection tubes 100
Resuspension solution, 25 ml 1
Lysis solution, 25 ml 1
Neutralization solution, 40 ml 1
5x wash solution, 25 ml 1
Elution solution, 16 ml 1
Instruction manual 1
DNA yield >20 µg
Preparation time 10 min
A200/A280 1.75–1.85
Bacterial density Up to OD 12
Purity >99% accuracy*
Purification format Vacuum and spin
x

Account price failed to display. Please try reloading the page.Reload Page

If you are placing an order, you may proceed with your order; the account price will be applied if it is lower than the
list price. If the problem persists, please call customer service at or send an
email to

Aurum Plasmid Mini Purification Kit

7326400EDU
Plasmid purification kit contains plasmid-binding mini columns, capless collection tubes, reagents, for 100 preps

0 7326400EDU /templatedata/internet/product_SKU/data/LSE/Cloning-and-Sequencing-Explore/Cloning-and-Sequencing-Explore/732-6400EDU_0921121012543.xml 7326400EDU /default/main/bio-rad/WORKAREA/default 09/21/09 12:10 PM en_US jc99dsmt 2017-11-14 07:07:14 true true internet/product_SKU jc99dsmt templatedata/internet/product_SKU/data/LSE/Cloning-and-Sequencing-Explore/Cloning-and-Sequencing-Explore/732-6400EDU_0921121012543.xml LSE 2019-05-04 11:13:12 /default/main/bio-rad/STAGING N /default/main/bio-rad en 12/31/37 11:59 PM 1555972631979 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN 2019-05-04 11:13:12 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSE/Cloning-and-Sequencing-Explore/Cloning-and-Sequencing-Explore/732-6400EDU_0921121012543.xml templatedata/internet/product_SKU/data/LSE/Cloning-and-Sequencing-Explore/Cloning-and-Sequencing-Explore/732-6400EDU_0921121012543.xml 7326400edu-aurum-plasmid-mini-purification-kit 7326400EDU Aurum Plasmid Mini Purification Kit Aurum Plasmid Mini Purification Kit Plasmid purification kit contains plasmid-binding mini columns, capless collection tubes, reagents, for 100 preps Plasmid purification kit contains plasmid-binding mini columns, capless collection tubes, reagents, for 100 preps Plasmid purification kit contains plasmid-binding mini columns, capless collection tubes, reagents, for 100 preps /webroot/web/images/lse/products/cloning_sequ_explorer_series/sku_thumb/global/732-6400EDU_tn.jpg /webroot/web/images/lse/products/cloning_sequ_explorer_series/sku_view/global/732-6400EDU_view.jpg /webroot/web/images/lse/products/cloning_sequ_explorer_series/sku_feature/global/732-6400EDU_feature.jpg Aurum&trade; Plasmid Mini Purification Kit #732-6400EDU RepEx Aurum&trade; Plasmid Mini Purification Kit #732-6400EDU Plasmid purification kit contains plasmid-binding mini columns, capless collection tubes, reagents, for 100 preps 7326400, 7326400EDU, aurum, plasmid, mini, dna, purification, sample, preparation, columns, capless, collection, tubes, reagents, science, education, products, classroom 09/21/09 12:10 PM 12/31/37 11:59 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSE /LSE/Cloning-and-Sequencing-Explore/Cloning-and-Sequencing-Explore N 0 ,VN <p>The seventh module in the Cloning and Sequencing Explorer Series, the Aurum&trade; Plasmid Mini Purification Module contains reagents to purify up to 20 &micro;g of DNA from a plasmid miniculture. The kit includes spin columns, collection tubes, buffers, and curriculum. This product is for education use only.</p> /webroot/web/html/lse/products/SKUs/autopopulated/ 732-6400EDU_0921121012543_en.html /webroot/web/html/lse/products/SKUs/autopopulated//732-6400EDU_0921121012543_en.html 1141 <b>Kit contains sufficient reagents to purify DNA from 100 minicultures of plasmid-bearing bacteria</b> &nbsp; 1 0 1580 DNA-binding columns 100 1 0 2700 Capless collection tubes, 2.0 ml 100 1 0 2373 Resuspension solution, 25 ml 1 1 0 2374 Lysis solution, 25 ml 1 1 0 2375 Neutralization solution, 40 ml 1 1 0 2701 Wash solution, 5x, 25 ml 1 1 0 2377 Elution solution, 16 ml 1 1 0 2398 Curriculum, including teacher's guide, student manual, and graphic quick guide 1 1 0 false $217.14 true $ %24152.00 true false true true false USD true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=7326400EDU&quantity= false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=7326400EDU&CartQuote=QT&quantity= 1711 false true false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=7326400EDU&AddToDefaultWishList=true&quantity= 41116100 217.14 7326400EDU
List Price: 
 
$217.14 
LoadingLoading...
EDU Price:
  $152.00  What is this?
Your Price: 
 
LoadingLoading...
Your Price:
 

Communication error

Reload Page

Quantity:   Add to Cart
 
 
Number Description Options
2664 Aurum Plasmid Mini Kit Product Information Sheet, Rev C Click to download
2671 Quick Guide, Aurum Vacuum Manifold Column Purification, Rev A Click to download
2663 Quick Guide, Aurum Plasmid Mini Kit Spin Format Protocol Overview, Rev A Click to download [ Add to Cart (Free) ]
4110111 Instruction Manual, Aurum Plasmid Mini Kit, Biotechnology Explorer, Rev C Click to download

This product contains electrical or electronic material. Learn more about Bio-Rad's EU Recycle Program

Get Help
Sign Up for Bio-Rad Updates!
Enter your email address below to receive your choice of the latest news, promotions, and more.
4b89c258-92a7-4dba-bd88-ac2418290a9b [x-forwarded-proto] = [https] [accept-language] = [en-US,en;q=0.5] [x-forwarded-port] = [443] [x-forwarded-for] = [3.83.192.109, 10.232.3.174] [accept] = [text/html,application/xhtml+xml,application/xml;q=0.9,*/*;q=0.8] [seourl] = [/en-us/product/aurum-plasmid-mini-purification-module] [x-amzn-trace-id] = [Root=1-5d61fe64-c6c160efd3d693b5725308c5] [x-forwarded-server] = [lsds-prod-s.br.aws-livesite.io] [x-forwarded-host] = [www.bio-rad.com] [x-query-string] = [ID=4b89c258-92a7-4dba-bd88-ac2418290a9b] [host] = [10.232.17.28:1776] [x-request-uri] = [/en-us/product/aurum-plasmid-mini-purification-module] [connection] = [Keep-Alive] [accept-encoding] = [gzip] [user-agent] = [CCBot/2.0 (https://commoncrawl.org/faq/)] Catalog/ProductDetail pageStyleKey aurum-plasmid-mini-purification-module LSE https://www.bio-rad-antibodies.com https://www.bio-rad.com 03/26/14 02:17 PM en_US true internet/generic 2019-07-09 16:59:23 /default/main/bio-rad/STAGING 1385504243813 US templatedata/internet/generic/data/en_US/GNL/page_generic_footer_b2c_1385505294_US.xml page_generic_footer_b2c /default/main/bio-rad/WORKAREA/default jc7v8okm 2017-10-27 13:13:40 true Done jc7v8okm templatedata/internet/generic/data/en_US/GNL/page_generic_footer_b2c_1385505294_US.xml GNL N /default/main/bio-rad en_US 11/27/23 02:18 PM 1561946660551 2019-07-09 16:59:23 /default/main/bio-rad/WORKAREA/default/templatedata/internet/generic/data/en_US/GNL/page_generic_footer_b2c_1385505294_US.xml footer 1 1385504243813 page_generic_footer_b2c <div class="col"> <h4>Support</h4> <ul> <li><a class="wtclass linkgeneration" href="_brcDomain/_locale/life-science-research/support?ID=1140">Life Science Support</a></li> <li><a class="wtclass linkgeneration" href="_acdDomain/technical-support.html">Antibodies Support</a></li> <li><a class="wtclass linkgeneration" href="_brcDomain/_locale/life-science-research/purchase-service-programs/expert-care-service?ID=1383780912356">Expert Care Service</a></li> <li><a class="wtclass linkgeneration" href="_brcDomain/contact">Contact Us</a></li> </ul> </div> <div class="col"> <h4>Documents</h4> <ul> <li><a class="wtclass linkgeneration" href="_brcDomain/_locale/literature-library">Literature Library</a></li> <li><a class="wtclass linkgeneration" href="_brcDomain/_locale/life-science-research/support/certificate-of-analysis">Certificate of Analysis</a></li> <li><a class="wtclass" href="https://myeinserts.qcnet.com/" target="_blank" rel="noopener noreferrer">QC Inserts</a></li> <li><a class="wtclass linkgeneration" href="_acdDomain/iso-9001-quality-assurance-management.html">Quality Certification</a></li> </ul> </div> <div class="col"> <h4>Ordering</h4> <ul> <li><a class="wtclass linkgeneration" href="_brcDomain/_locale/my-bio-rad">Quick Order</a></li> <li><a id="footer_orderLink" class="orderhistoryLink wtclass" href="javascript:void(0);">Order History</a></li> <li><a class="wtclass" id="footer_quoteLink" href="javascript:void(0);">Quote History</a></li> <li><a class="wtclass linkgeneration" href="_brcDomain/_locale/my-bio-rad">My Bio-Rad</a></li> <li><a class="wtclass linkgeneration" href="_brcDomain/_locale/life-science-research/purchase-service-programs/supply-center-program?ID=1383773882551">Supply Centers</a></li> </ul> </div> <div class="col"> <h4>Our Products</h4> <ul> <li><a class="wtclass linkgeneration" href="_brcDomain/_locale/life-science-research?ID=1100">Life Science Research</a></li> <li><a class="wtclass linkgeneration" href="_acdDomain/">Antibodies</a></li> <li><a class="wtclass linkgeneration" href="_brcDomain/_locale/clinical-diagnostics?ID=1200">Clinical Diagnostics</a></li> <li><a class="wtclass linkgeneration" href="_brcDomain/_locale/spectroscopy?ID=1300">Spectroscopy</a></li> <li><a class="wtclass linkgeneration" href="_brcDomain/_locale/process-separations?ID=1400">Process Separations</a></li> <li><a class="wtclass linkgeneration" href="_brcDomain/_locale/food-science?ID=1475">Food Science</a></li> <li><a class="wtclass linkgeneration" href="_brcDomain/_locale/education?ID=1450">Life Science Education</a></li> </ul> </div> <div class="col"> <h4>Company</h4> <ul> <li><a class="wtclass linkgeneration" href="_brcDomain/_locale/corporate/about-bio-rad?ID=1003">About Bio-Rad</a></li> <li><a class="wtclass linkgeneration" href="_brcDomain/_locale/corporate/careers?ID=1004">Careers</a></li> <li><a class="wtclass linkgeneration" href="_brcDomain/_locale/corporate/investor-relations?ID=1007">Investor Relations</a></li> <li><a class="wtclass linkgeneration" href="_brcDomain/_locale/corporate/newsroom?ID=1006">Newsroom</a></li> <li><a class="wtclass linkgeneration" href="_acdDomain/blog/">Blog</a></li> <li><a class="wtclass" target="_blank" href="https://www.bioradiations.com" rel="noopener noreferrer">Bioradiations</a></li> <li> <div class="social"> <ul> <li><a class="no-target-style brconly wtclass" href="https://www.linkedin.com/company/bio-rad?trk=top_nav_home" target="_blank" rel="noopener noreferrer"><img src="https://www.bio-rad.com/webroot/web/images/general/icons/linkedin_round_40x40.png" alt="Bio-Rad LinkedIn" width="20" height="20" /></a> <a class="no-target-style bracd wtclass" style="display: none;" href="https://www.linkedin.com/company/bio-rad-antibodies" target="_blank" rel="noopener noreferrer"><img src="https://www.bio-rad.com/webroot/web/images/general/icons/linkedin_round_40x40.png" alt="Bio-Rad Antibodies LinkedIn" width="20" height="20" /></a></li> <li><a class="no-target-style brconly wtclass" href="https://www.youtube.com/user/BioRadLifeScience" target="_blank" rel="noopener noreferrer"><img src="https://www.bio-rad.com/webroot/web/images/general/icons/youtube-icon-rnd-40x40.png" alt="Bio-Rad YouTube" width="20" height="20" /></a> <a class="no-target-style bracd wtclass" style="display: none;" href="https://www.youtube.com/playlist?list=PLrAEgIY86I6w1jajKp3O9RQJEvJZ2ux0I" target="_blank" rel="noopener noreferrer"><img src="https://www.bio-rad.com/webroot/web/images/general/icons/youtube-icon-rnd-40x40.png" alt="Bio-Rad Antibodies YouTube" width="20" height="20" /></a></li> <li><a class="no-target-style brconly wtclass" href="https://twitter.com/BioRadLifeSci" target="_blank" rel="noopener noreferrer"><img src="https://www.bio-rad.com/webroot/web/images/general/icons/twitter_round_40x40.png" alt="Bio-Rad Twitter" width="20" height="20" /></a> <a class="no-target-style bracd wtclass" style="display: none;" href="https://twitter.com/BioRadAbs" target="_blank" rel="noopener noreferrer"><img src="https://www.bio-rad.com/webroot/web/images/general/icons/twitter_round_40x40.png" alt="Bio-Rad Antibodies Twitter" width="20" height="20" /></a></li> <li><a class="no-target-style brconly wtclass" href="https://www.facebook.com/biorad/" target="_blank" rel="noopener noreferrer"><img src="https://www.bio-rad.com/webroot/web/images/general/icons/facebook-icon-rnd-40x40.png" alt="Bio-Rad Facebook" width="20" height="20" /></a> <a class="no-target-style bracd wtclass" style="display: none;" href="https://www.facebook.com/BioRadAntibodies" target="_blank" rel="noopener noreferrer"><img src="https://www.bio-rad.com/webroot/web/images/general/icons/facebook-icon-rnd-40x40.png" alt="Bio-Rad Antibodies Facebook" width="20" height="20" /></a></li> <li><a class="no-target-style wtclass" href="https://www.instagram.com/bioradlabs/" target="_blank" rel="noopener noreferrer"><img src="https://www.bio-rad.com/webroot/web/images/general/icons/instagram-icon-rnd-40x40.png" alt="Bio-Rad Instagram" width="20" height="20" /></a></li> <li><a class="no-target-style wtclass" href="https://uk.pinterest.com/BioRadAbs/" target="_blank" rel="noopener noreferrer"><img src="https://www.bio-rad.com/webroot/web/images/general/icons/pinterest40x40.png" alt="Bio-Rad Pinterest" width="20" height="20" /></a></li> </ul> </div> </li> </ul> </div> /webroot/web/html footer_page_b2c_en_US.html /webroot/web/html/footer_page_b2c_en_US.html Karen Moss Footer page Footer 03/26/14 02:17 PM 11/27/23 02:18 PM US en GNL /GNL N 0 en-us Home <script type="text/javascript">$(document).ready(function() { var region = $("#headlangID .lang strong").html(); $(".footer-nav > a:nth-child(3)").after(" | <a href='https://www.bio-rad.com/webroot/web/html/featured/en/web-accessibility.html' id='regionlink'>Web Accessibility</a>");});</script>Trademarks Site Terms Terms & Conditions Imprint EU Recycle Program Privacy Change Cookie Setting <script type="text/javascript">$(document).ready(function () {$(".links2").append('<style>.social-links-br {float: left;margin-right: 10px;}.social-links-br img {width: 20px;height: 20px;margin-right: 5px}</style><div class="social-links-br"><a class="no-target-style" href="https://www.linkedin.com/company/bio-rad?trk=top_nav_home" target="_blank"><img src="/webroot/web/images/general/icons/linkedin_round_40x40.png" alt="Bio-Rad LinkedIn"></a><a class="no-target-style" href="https://www.youtube.com/user/BioRadLifeScience" target="_blank"><img src="/webroot/web/images/general/icons/youtube-icon-rnd-40x40.png" alt="Bio-Rad YouTube"></a><a class="no-target-style" href="https://twitter.com/BioRadLifeSci" target="_blank"><img src="/webroot/web/images/general/icons/twitter_round_40x40.png" alt="Bio-Rad Twitter"></a><a class="no-target-style" href="https://www.facebook.com/biorad/" target="_blank"><img src="/webroot/web/images/general/icons/facebook-icon-rnd-40x40.png" alt="Bio-Rad Facebook"></a><a class="no-target-style" href="https://www.instagram.com/bioradlabs/" target="_blank"><img src="/webroot/web/images/general/icons/instagram-icon-rnd-40x40.png" alt="Bio-Rad Instagram"></a></div>');});</script>Copyright &copy; {0} Bio-Rad Laboratories, Inc. All rights reserved. This page was last edited on {0} true True GTM-MJ2DN2S us US en LSE education life-science-research /prd/en/US/direct/biorad?ts=1&cmd=OrdersWorkspaceDisplay /prd/en/US/direct/biorad?ts=1&cmd=QuotesWorkspaceDisplay /prd/en/US/direct/biorad?ts=1&cmd=InvoiceWorkspaceDisplay&_Tab=Invoice false false true false true true 1040988879 LHsYCOu_wQMQz_Ww8AM window.google_tag_params true 272-THL-329 username CartID_ WT_FPC <script type="text/javascript">$.ajax({url: document.location.protocol + '//munchkin.marketo.net/munchkin.js',dataType: 'script',cache: true,success: function() {Munchkin.init('044-CBN-599');}});</script> <script type="text/javascript" src="https://www.bio-rad.com/evportal/framework/skins/evolution/js/proxyMunchkin.js"></script> 2019 {"country":"us","b2b_punch_out_mode":"","pageType":"Product Detail Page","user_id":"","visitorLoginState":false,"visitorType":"","sessionType":"B2C","language":"en","vertical":"LSE","b2b_org_id":""}