Aurum™ Plasmid Mini Purification Module

Image
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Overview

In this module, students can rapidly purify up to 20 µg of plasmid DNA from transformed bacteria using a three-step alkaline lysis procedure followed by mini column chromatography.

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Description

Description

Purify up to 20 µg of plasmid DNA rapidly and inexpensively without the use of toxic reagents or alcohol precipitations. Plasmid DNA is purified from transformed bacteria using a three-step alkaline lysis procedure followed by mini column chromatography. The highly purified plasmid DNA is free of salts, bacterial chromosomal DNA, and RNA, and is ideal for subsequent molecular biology–based applications such as automated sequencing, cloning, PCR, or restriction digestion analysis.

The Aurum Plasmid Mini Purification Module is an integral component of the Cloning and Sequencing Explorer Series. The module may also be purchased separately and used to isolate plasmid from bacteria containing any cloned gene.

Specifications

Specifications

Kit contains sufficient reagents to purify
DNA from 100 minicultures of plasmid-bearing
bacteria.
 
DNA-binding mini columns
100
Capless collection tubes
100
Resuspension solution, 25 ml
1
Lysis solution, 25 ml
1
Neutralization solution, 40 ml
1
5x wash solution, 25 ml
1
Elution solution, 16 ml
1
Instruction manual
1
DNA yield
>20 µg
Preparation time
10 min
A200/A280
1.75–1.85
Bacterial density
Up to OD 12
Purity
>99% accuracy*
Purification format
Vacuum and spin

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7326400EDU
Plasmid purification kit contains plasmid-binding mini columns, capless collection tubes, reagents, for 100 preps

Documents

Documents

Number Description Options
2664
Aurum Plasmid Mini Kit Product Information Sheet, Rev C
2671
Quick Guide, Aurum Vacuum Manifold Column Purification, Rev A
2663
Quick Guide, Aurum Plasmid Mini Kit Spin Format Protocol Overview, Rev A
4110111
Instruction Manual, Aurum Plasmid Mini Kit, Biotechnology Explorer, Rev C