Ligation and Transformation Module

Overview

Kit contains sufficient materials for 12 workstations.
T4 DNA ligase, 10 µl	1
2x ligase buffer, 100 µl	1
Proofreading DNA polymerase, 10 µl	1
Cloning vector, 10 µl	1
Bgl II restriction enzyme, 50 µl	1
10x restriction enzyme buffer, 100 µl	1
C-Growth medium, 20 ml	1
Transformation A solution, 5 ml	1
Transformation B solution, 5 ml	1
IPTG, 0.1 ml	1
Sterile water, 2.5 ml	1
Colored micro test tubes, 2.0 ml	120
Curriculum, including Teacher's Guide, Student Manual, and graphic Quick Guide	1
Required Accessories Not Included in Kit:
Water bath
Microcentrifuge
Incubation oven
Shaking water bath

Note: Bacterial culturing reagents such as LB broth and LB agar are contained in the Microbial Culturing module.

Ligation and Transformation

Ligate the PCR product into a plasmid and transform bacteria. Students directly clone their PCR products into the pJET1.2 vector and immediately transform bacteria using a protocol that takes less than 2 hours to go from purified PCR product to transformed bacteria plated on agar.

Students first remove the 3'-dA overhang from their PCR product which results from amplification with Taq polymerase using a proofreading polymerase. A 10 minute protocol is then used to ligate the blunted PCR product into a pre-opened and blunted vector — pJET1.2. The pJET1.2 plasmid positively selects for successful insertion of fragments because the cloned fragment inserts into a lethal gene present in the plasmid that prevents its activation. Bacteria that are transformed with re-ligated vector activate the gene and are killed. This results in higher transformation efficiency than traditional blue-white cloning. Bgl II restriction sites are located on either side of the pJET1.2 cloning site, which allow students to determine if their gene of interest was successfully ligated once they have isolated candidate plasmids.

To transform the ligation into bacteria, students make competent cells by inoculating specialized growth media and performing a series of microcentrifugations and washes in a specialized transformation buffer. Competent cells are then added to the ligations on ice and plated directly on warm agar plates. Between 10 and 100 colonies should be expected from transformed ligations with the Cloning and Sequencing Explorer Series.

This module allows students to directly ligate purified PCR products into the pJET1.2 plasmid vector and immediately transform freshly prepared competent bacteria with the ligation reaction.

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Description

The ligation and transformation module is an integral component of the Cloning and Sequencing Explorer Series. Use this module to directly ligate PCR products into the pJET1.2 plasmid vector and immediately transform freshly prepared competent bacteria with the ligation reaction. Competent cells are then plated directly on warm agar plates and incubated overnight at 37°C.

Once transformed bacteria have been propagated, plasmid DNA can be isolated and digested with BglII enzyme. The BglII restriction sites located on either side of the pJET1.2 cloning site allow students to determine if their PCR product was successfully ligated.

This module may also be purchased separately and used to clone any PCR product. The entire ligation and transformation protocol takes approximately 2 hours to complete and does not require commercial competent cells, a refrigerated microcentrifuge, or a –80°C freezer, making it the method of choice for educators worldwide.

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Ligation And Transformation Module

1665015EDU
Fifth module in Cloning and Sequencing Explorer Series classroom study kit, includes T4 ligase, cloning vector, growth medium, curriculum, and more, for 12 workstations; education use only

0 1665015EDU /templatedata/internet/product_SKU/data/LSE/Cloning-and-Sequencing-Explore/Cloning-and-Sequencing-Explore/166-5015EDU_0921121012507.xml 1665015EDU /default/main/bio-rad/WORKAREA/default 09/21/09 12:10 PM en_US jc99dso6 2017-11-14 07:07:14 true true internet/product_SKU jc99dso6 templatedata/internet/product_SKU/data/LSE/Cloning-and-Sequencing-Explore/Cloning-and-Sequencing-Explore/166-5015EDU_0921121012507.xml LSE 2019-05-04 11:13:22 /default/main/bio-rad/STAGING N /default/main/bio-rad en 12/31/37 11:59 PM 1555972631811 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN 2019-05-04 11:13:22 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSE/Cloning-and-Sequencing-Explore/Cloning-and-Sequencing-Explore/166-5015EDU_0921121012507.xml templatedata/internet/product_SKU/data/LSE/Cloning-and-Sequencing-Explore/Cloning-and-Sequencing-Explore/166-5015EDU_0921121012507.xml 1665015edu-ligation-transformation-module 1665015EDU Ligation And Transformation Module Ligation And Transformation Module Fifth module in Cloning and Sequencing Explorer Series classroom study kit, includes T4 ligase, cloning vector, growth medium, curriculum, and more, for 12 workstations; education use only Fifth module in Cloning and Sequencing Explorer Series classroom study kit, includes T4 ligase, cloning vector, growth medium, curriculum, and more, for 12 workstations; education use only Fifth module in Cloning and Sequencing Explorer Series classroom study kit, includes T4 ligase, cloning vector, growth medium, curriculum, and more, for 12 workstations; education use only /webroot/web/images/lse/products/cloning_sequ_explorer_series/sku_thumb/global/166-5015EDU_tn.jpg /webroot/web/images/lse/products/cloning_sequ_explorer_series/sku_view/global/166-5015EDU_view.jpg /webroot/web/images/lse/products/cloning_sequ_explorer_series/sku_feature/global/166-5015EDU_feature.jpg Ligation and Transformation Module #166-5015EDU RepEx Ligation and Transformation Module #166-5015EDU Fifth module in Cloning and Sequencing Explorer Series classroom study kit, includes T4 ligase, cloning vector, growth medium, curriculum, and more, for 12 workstations; education use only 1665015, 1665015EDU, ligation, and, transformation, module, fifth, cloning, sequencing, explorer, series, ligase, buffer, proofreading, dna, polymerase, vector, science, education, products, classroom 09/21/09 12:10 PM 12/31/37 11:59 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSE /LSE/Cloning-and-Sequencing-Explore/Cloning-and-Sequencing-Explore N 0 ,VN <p>The fifth module in Cloning and Sequencing Explorer Series, the Ligation and Transmformation Module allows the ligation of PCR products into the pJET1.2 plasmid vector for transformation of competent bacteria. The kit includes T4 ligase, cloning vector, proofreading polymerase, buffers, C-Growth medium, transformation reagents, microcentrifuge tubes, and curriculum. Materials are sufficient for 12 student workstations. This product is for education use only.</p> /webroot/web/html/lse/products/SKUs/autopopulated/ 166-5015EDU_0921121012507_en.html /webroot/web/html/lse/products/SKUs/autopopulated//166-5015EDU_0921121012507_en.html 1139 <b>Kit contains sufficient materials for 12 student workstations (2–4 students per workstation)</b>   1 0 2691 T4 DNA ligase, 10 µl 1 1 0 2692 Ligation reaction buffer, 2x, 100 µl 1 1 0 2693 Proofreading DNA polymerase, 10 µl 1 1 0 2694 pJET1.2 blunted cloning vector, 10 µl 1 1 0 1380 BgIII restriction enzyme, 55 µl 1 1 0 2695 Restriction enzyme buffer, 10x, 100 µl 1 1 0 1397 C-Growth medium, 30 ml 1 1 0 2696 Transformation reagent A, 5 ml 1 1 0 2697 Transformation reagent B, 5 ml 1 1 0 2698 IPTG ,1 M, 0.1 ml 1 1 0 2699 Sterile water, 1 ml 1 1 0 2421 Colored microcentrifuge tubes, 2.0 ml 120 1 0 2428 Microcentrifuge tubes, 1.5 ml 30 1 0 2398 Curriculum, including teacher's guide, student manual, and graphic quick guide 1 1 0 false $406.25 true $ %24325.00 true false true true false USD true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1665015EDU&quantity= false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1665015EDU&CartQuote=QT&quantity= 1711 false true false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1665015EDU&AddToDefaultWishList=true&quantity= 41106600 406.25 Product detail page 1665015EDU

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Refills

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Ligation Module Reagent Refill

1665016EDU
Consumables refill kit for use with Ligation and Transformation module (#166-5015EDU), contains T4 DNA ligase, ligase buffer, proofreading DNA polymerase, cloning vector, and more; education use only

1665016EDU /templatedata/internet/product_SKU/data/LSE/Cloning-and-Sequencing-Explore/Ligation-and-Transformation-Mo/166-5016EDU_0921121013278.xml 2 1665016EDU /default/main/bio-rad/WORKAREA/default 09/21/09 12:10 PM en_US jcrucx04 2017-11-14 07:07:15 true true internet/product_SKU jcrucx04 templatedata/internet/product_SKU/data/LSE/Cloning-and-Sequencing-Explore/Ligation-and-Transformation-Mo/166-5016EDU_0921121013278.xml LSE 2019-05-04 11:13:10 /default/main/bio-rad/STAGING N /default/main/bio-rad en 12/31/37 11:59 PM 1555972632652 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN 2019-05-04 11:13:10 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSE/Cloning-and-Sequencing-Explore/Ligation-and-Transformation-Mo/166-5016EDU_0921121013278.xml templatedata/internet/product_SKU/data/LSE/Cloning-and-Sequencing-Explore/Ligation-and-Transformation-Mo/166-5016EDU_0921121013278.xml 1665016edu-ligation-module-reagent-refill 1665016EDU Ligation Module Reagent Refill Ligation Module Reagent Refill Consumables refill kit for use with Ligation and Transformation module (#166-5015EDU), contains T4 DNA ligase, ligase buffer, proofreading DNA polymerase, cloning vector, and more; education use only Consumables refill kit for use with Ligation and Transformation module (#166-5015EDU), contains T4 DNA ligase, ligase buffer, proofreading DNA polymerase, cloning vector, and more; education use only Consumables refill kit for use with Ligation and Transformation module (#166-5015EDU), contains T4 DNA ligase, ligase buffer, proofreading DNA polymerase, cloning vector, and more; education use only /webroot/web/images/lse/products/cloning_sequ_explorer_series/sku_thumb/global/166-5016EDU_tn.jpg /webroot/web/images/lse/products/cloning_sequ_explorer_series/sku_view/global/166-5016EDU_view.jpg /webroot/web/images/lse/products/cloning_sequ_explorer_series/sku_feature/global/166-5016EDU_feature.jpg RepEx 166-5016EDU Consumables refill kit for use with Ligation and Transformation module (#166-5015EDU), contains T4 DNA ligase, ligase buffer, proofreading DNA polymerase, cloning vector, and more; education use only 09/21/09 12:10 PM 12/31/37 11:59 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSE /LSE/Cloning-and-Sequencing-Explore/Ligation-and-Transformation-Mo N 0 false $185.00 true $ %24148.00 true false true true false USD true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1665016EDU&quantity= false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1665016EDU&CartQuote=QT&quantity= 1711 false true false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1665016EDU&AddToDefaultWishList=true&quantity= 41106600 185.00 Product detail page 1665016EDU

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1665016EDU Add to Hot List		Add to Quote 185.00

Transformation Module Reagent Refill

1665017EDU
Consumables refill kit for use with Ligation and Transformation module (#166-5015EDU), contains Transformation A solution, Transformation B solution, C-Growth medium, and IPTG; education use only

1665017EDU /templatedata/internet/product_SKU/data/LSE/Cloning-and-Sequencing-Explore/Ligation-and-Transformation-Mo/166-5017EDU_0921121013316.xml 3 1665017EDU /default/main/bio-rad/WORKAREA/default 09/21/09 12:10 PM en_US jcrucwzx 2017-11-14 07:07:15 true true internet/product_SKU jcrucwzx templatedata/internet/product_SKU/data/LSE/Cloning-and-Sequencing-Explore/Ligation-and-Transformation-Mo/166-5017EDU_0921121013316.xml LSE 2019-05-04 11:13:03 /default/main/bio-rad/STAGING N /default/main/bio-rad en 12/31/37 11:59 PM 1555972632694 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN 2019-05-04 11:13:03 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSE/Cloning-and-Sequencing-Explore/Ligation-and-Transformation-Mo/166-5017EDU_0921121013316.xml templatedata/internet/product_SKU/data/LSE/Cloning-and-Sequencing-Explore/Ligation-and-Transformation-Mo/166-5017EDU_0921121013316.xml 1665017edu-transformation-module-reagent-refill 1665017EDU Transformation Module Reagent Refill Transformation Module Reagent Refill Consumables refill kit for use with Ligation and Transformation module (#166-5015EDU), contains Transformation A solution, Transformation B solution, C-Growth medium, and IPTG; education use only Consumables refill kit for use with Ligation and Transformation module (#166-5015EDU), contains Transformation A solution, Transformation B solution, C-Growth medium, and IPTG; education use only Consumables refill kit for use with Ligation and Transformation module (#166-5015EDU), contains Transformation A solution, Transformation B solution, C-Growth medium, and IPTG; education use only /webroot/web/images/lse/products/cloning_sequ_explorer_series/sku_thumb/global/166-5017EDU_tn.jpg /webroot/web/images/lse/products/cloning_sequ_explorer_series/sku_view/global/166-5017EDU_view.jpg /webroot/web/images/lse/products/cloning_sequ_explorer_series/sku_feature/global/166-5017EDU_feature.jpg RepEx 166-5017EDU Consumables refill kit for use with Ligation and Transformation module (#166-5015EDU), contains Transformation A solution, Transformation B solution, C-Growth medium, and IPTG; education use only 09/21/09 12:10 PM 12/31/37 11:59 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSE /LSE/Cloning-and-Sequencing-Explore/Ligation-and-Transformation-Mo N 0 false $193.75 true $ %24155.00 true false true true false USD true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1665017EDU&quantity= false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1665017EDU&CartQuote=QT&quantity= 1711 false true false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1665017EDU&AddToDefaultWishList=true&quantity= 41106600 193.75 Product detail page 1665017EDU

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1665017EDU Add to Hot List		Add to Quote 193.75

Bgl II Reagent Refill

1665018EDU
Consumables refill kit, contains Bgl II restriction enzyme, restriction enzyme buffer; education use only

1665018EDU /templatedata/internet/product_SKU/data/LSE/Cloning-and-Sequencing-Explore/Ligation-and-Transformation-Mo/166-5018EDU_0921121013297.xml 4 1665018EDU /default/main/bio-rad/WORKAREA/default 09/21/09 12:10 PM en_US jcrucx0e 2017-11-14 07:07:15 true true internet/product_SKU jcrucx0e templatedata/internet/product_SKU/data/LSE/Cloning-and-Sequencing-Explore/Ligation-and-Transformation-Mo/166-5018EDU_0921121013297.xml LSE 2019-05-04 11:13:01 /default/main/bio-rad/STAGING N /default/main/bio-rad en 12/31/37 11:59 PM 1555972632736 AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN 2019-05-04 11:13:01 /default/main/bio-rad/WORKAREA/default/templatedata/internet/product_SKU/data/LSE/Cloning-and-Sequencing-Explore/Ligation-and-Transformation-Mo/166-5018EDU_0921121013297.xml templatedata/internet/product_SKU/data/LSE/Cloning-and-Sequencing-Explore/Ligation-and-Transformation-Mo/166-5018EDU_0921121013297.xml 1665018edu-bgl-ii-reagent-refill 1665018EDU Bgl II Reagent Refill Bgl II Reagent Refill Consumables refill kit, contains Bgl II restriction enzyme, restriction enzyme buffer; education use only Consumables refill kit, contains Bgl II restriction enzyme, restriction enzyme buffer; education use only Consumables refill kit, contains Bgl II restriction enzyme, restriction enzyme buffer; education use only /webroot/web/images/lse/products/cloning_sequ_explorer_series/sku_thumb/global/166-5018EDU_tn.jpg /webroot/web/images/lse/products/cloning_sequ_explorer_series/sku_view/global/166-5018EDU_view.jpg /webroot/web/images/lse/products/cloning_sequ_explorer_series/sku_feature/global/166-5018EDU_feature.jpg RepEx 166-5018EDU Consumables refill kit, contains Bgl II restriction enzyme, restriction enzyme buffer; education use only 09/21/09 12:10 PM 12/31/37 11:59 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA,VN en LSE /LSE/Cloning-and-Sequencing-Explore/Ligation-and-Transformation-Mo N 0 false $57.50 true $ %2446.00 true false true true false USD true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1665018EDU&quantity= false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1665018EDU&CartQuote=QT&quantity= 1711 false true false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1665018EDU&AddToDefaultWishList=true&quantity= 12352200 57.50 Product detail page 1665018EDU

List Price:		$57.50 Loading
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1665018EDU Add to Hot List		Add to Quote 57.50

Documents

Number	Description	Options
1665019	Instruction Manual, Ligation and Transformation Module, Biotechnology Explorer, Rev A	Click to download

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The lot number or control number (one or the other, not both) is printed on your product label. See the sample label below for the location of this information.

Why are Certificates of Analysis not on the Documents tab?

Certificates of Analysis are associated not only with a product, but also with a specific lot of that product. For a given product, there may be several Certificates of Analysis, especially if it is an older product line and has had several lots produced over the years. Using the Certificate of Analysis search tool, you can enter the catalog number and lot/control number for the specific product you have on hand and download the exact Certificate of Analysis you need.

I have a batch number, not a lot/control number. How do I find the Certificate of Analysis for my item?

You can use the batch number in place of the lot/control number. Use the Certificate of Analysis search tool, entering the catalog number as usual and the batch number where the lot/control number would go.

Can I get a Certificate of Analysis even if my item is expired?

Yes. While we do periodically remove Certificates of Analysis for site maintenance purposes, we typically keep them available well beyond a product's expiration date.

Why does it say "Product Name Not Found" for a Certificate of Analysis listed in the search results?

There are Certificates of Analysis that correspond to products that are either discontinued or simply not available on the website. In these situations, the Certificate of Analysis is available for download, but other product details, such as the product name, are not.

Why does it say "Not Available" for a Certificate of Analysis listed in the search results?

In this case, you have entered a valid catalog number and lot/control number and we have located the Certificate of Analysis. However, for some reason, the actual file itself is unavailable.

Please use the Request Form here or contact your local Bio-Rad Representative so that we can send you the Certificate of Analysis.

Find a Certificate of Analysis

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