Sequencing and Bioinformatics Module

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Overview

The sequencing and bioinformatics module is used to analyze a portion of the GAPC gene isolated from a plant. Students are guided through a sequence analysis that allows them to make a real contribution to science!

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Description

Description

The Sequencing and Bioinformatics Module is an integral component of the Cloning and Sequencing Explorer Series. This module is used to sequence and analyze a portion of the GAPC gene, a housekeeping gene in the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) family, from a plant of each student's choice. Once sequence data are generated* and verified, they can be uploaded into NCBI's GenBank database and used by other scientists.

This module combines the power of computer analysis with student-generated cloning data to teach students essential bioinformatics skills. The Sequencing and Bioinformatics Module includes a 3-month subscription for unrestricted access to Geneious Prime, a software program that provides students with the latest tools in bioinformatics data analysis.

The Sequencing and Bioinformatics Module can also be purchased separately and used to sequence any PCR product that is cloned into the pJET1.2 plasmid. Four sequencing primers are provided to sequence PCR fragments up to 1 kb in length. Once the sequence of the cloned gene has been obtained, use Geneious to assemble the sequences into a contig and analyze the data.

*Sequencing service is not included.

Specifications

Specifications

Kit contains reagents sufficient materials for 12 student workstations or 96 sequencing reactions
 
pJETF primer
1
pJETR primer
1
GAPSEQF primer
1
GAPSEQR primer
1
Control plasmid
1
Barcoded 96-well plate
1
Sealing film
1
Colored microcentrifuge tubes, 2 ml
60
Curriculum, including instruction manual
1

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166-5025EDU_tn.jpg
1665025EDU
Eighth module in Cloning and Sequencing Explorer Series classroom study kit, includes primers, plasmid, barcoded 96-well plate, test tubes, curriculum, and more, for 12 workstations; education use only (Sequencing service not included)

Refills

Refills

166-5026EDU_tn.jpg
1665026EDU
Consumables refill kit for use with Sequencing and Bioinformatics module (#166-5025EDU), contains pJETF, pJETR, GAPSEQF, and GAPSEQR primers, and control plasmid; education use only (Sequencing service not included)

Documents

Documents

Number Description Options
10000147502
Instruction Manual, Sequencing and Bioinformatics