Overview
| Kit contains sufficient materials for 12 workstations, or 25 genomic DNA extractions | |
| Lysis buffer, 20 ml | 1 |
| Dithiothreitol (DTT), 0.3 g | 1 |
| Wash buffer, 20 ml | 1 |
| Sterile water, 2.5 ml | 1 |
| Micropestles | 25 |
| Spin columns | 25 |
| Microcentrifuge tubes, 2.0 ml | 25 |
| Microcentrifuge tubes, 1.5 ml | 30 |
| Colored microcentrifuge tubes, 2.0 ml | 60 |
| Instruction manual | 1 |
| Required Accessories Not Included in Kit: | |
| Plant samples | |
| 95–100% lab grade ethanol | |
| Adjustable micropipets | |
| 2–20 µl | 1 |
| 20–200 µl | 1–12 |
| 100–1,000 µl | 1–12 |
| Pipet tips, aerosol barrier | |
| 2–20 µl, Xcluda™ B | 1 box |
| 20–200 µl, Xcluda D | 1 box |
| 100–1,000 µl, Xcluda E | 1 box |
| Microcentrifuges capable of greater than 12,000 x g | 1–4 |
| Water bath | 1 |
| Heat block | 1 |
| Recommended (Optional) Accessories: | |
| Vortexer | |
| Spectrophotometer and cuvettes | |
| Fluorometer (VersaFluor™) | |
| DEPC-treated water, 100 ml | |
| Refills Available Separately: | |
| Nucleic Acid Extraction Refill Kit, includes DTT, lysis buffer, wash buffer, sterile water |
|
Prepare your students for the real world of scientific research. Engage them with the opportunity to perform novel, relevant research that can actually contribute to scientific knowledge. In this unique modular lab series, students are guided through an innovative research workflow identical to those performed in genomics labs worldwide. Over a multiple-week lab course, students will combine traditional and cutting edge molecular biology techniques and bioinformatics to clone, sequence, and analyze a housekeeping gene from a plant of your choice ensuring each class produces unique and novel data. The real thing. As a research and diagnostics manufacturing company, Bio-Rad brings unique strengths to the education market, including credibility, high quality products (kits and equipment that work), novel inquiry-based curricula, cutting edge kit applications, competitive pricing, superior teacher professional development programs, long-term strategic partners and allies, and strong personal endorsements from customers across all levels of education.
The first step in a gene cloning lab, the Nucleic Acid Extraction module allows students to extract all nucleic acids from plant cells using tissue grinding and lysis, followed by purification using column chromatography.
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