Nucleic Acid Extraction Module

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Overview

The first step in a gene cloning lab, the Nucleic Acid Extraction module allows students to extract all nucleic acids from plant cells using tissue grinding and lysis, followed by purification using column chromatography.

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Description

Description

To clone a gene, genomic DNA (gDNA) must first be extracted from the organism. The nucleic acid extraction module allows students to extract all nucleic acids from plant cells using tissue grinding and lysis, followed by purification using column chromatography. The final sample, which contains gDNA and RNA, can be used for PCR. RNA can then be removed from the sample by treatment with RNase I as an optional activity.

The protocol provided with this module has been optimized for genomic DNA extraction from most plant species. Different plants yield different quantities of genomic DNA. Each extraction yields sufficient DNA for 16 PCR amplifications.

If you want students to use the nucleic acid extraction module to extract RNA, genomic DNA can be removed from the sample with DNase I treatment. For further RNA work, elution using DEPC-treated water is recommended.

Specifications

Specifications

Kit contains sufficient materials for 25 genomic DNA extractions
 
Lysis buffer
20 ml
Dithiothreitol (DTT)
0.3 g
Wash buffer
20 ml
Sterile water
2.5 ml
Micropestles
25
Spin columns
25
Microcentrifuge tubes
2.0 ml, 25
Microcentrifuge tubes
1.5 ml, 30
Colored microcentrifuge tubes
2.0 ml, 60
Instruction manual
1

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1665005EDU
First module in Cloning and Sequencing Explorer Series classroom study kit, includes buffers, DTT, test tubes

Refills

Refills

166-5006EDU_tn.jpg
1665006EDU
Consumables refill kit for use with Nucleic Acid Extraction module (#166-5005EDU), contains DTT, lysis buffer, wash buffer, sterile water; education use only

Documents

Documents

Number Description Options
1665007
Instruction Manual, Nucleic Acid Extraction Module, Biotechnology Explorer, Rev A