UNOsphere™ Cation Exchange Resin

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UNOsphere Cation Exchange Resin - Cation Exchange Resin

Overview

Specifications
  UNOsphere™ Q UNOsphere S
Type of ion exchanger Strong anion Strong cation
Functional group -N+(CH3)3 -SO3-
Total ionic capacity 120 µeq/ml 280 µeq/ml
Dynamic binding capacity*    
   150 cm/hr 180 mg BSA/ml 60 mg IgG/ml
   600 cm/hr 125 mg BSA/ml 30 mg IgG/ml
Shipping counterion Cl- Na+
Median particle size 120 µm 80 µm
Recommended linear flow rate range** 50–1,200 cm/hr 50–1,200 cm/hr
Chemical stability    
   1.0 M NaOH (20°C) Up to 10,000 hr Up to 10,000 hr
   1.0 M HCl (20°C) Up to 200 hr Up to 200 hr
Volume changes**    
   pH 4–10 <5% <5%
   0.01–1.0 M NaCl <5% <5%
pH stability 1–14 1–14
Autoclavability Yes Yes
Antimicrobial agent 20% ethanol 20% ethanol
Regeneration 1–2 M NaCl 1–2 M NaCl
Storage conditions 20% ethanol or 0.1 M NaOH 20% ethanol or 0.1 M NaOH

* 10% breakthrough capacity determined with 4.5 mg/ml human IgG (UNOsphere S) and 2.0 mg/ml BSA (UNOsphere Q) in a 1.1 x 20 cm column.
** UNOshpere packed into a 20 cm bed height and run at 1,200 cm/hr generates less than 2 bar backpressure.

UNOsphere ion exchange resins are designed to meet the increasing needs of the biopharmaceutical industry for high-productivity chromatographic resin. UNOsphere, a hydrophilic polymeric support, delivers high productivity through high binding capacity and low backpressures. The single-step polymerization process produces a support unsurpassed in quality and batch-to-batch reproducibility.

UNOsphere S support is used for strong cation exchange chromatography. UNOsphere Rapid S resin builds on the properties and features of the UNOsphere family of supports with enhanced chemistries that overcome the pH shift that occurs with conductivity transitions.

Key Features

  • Efficient capture of biopharmaceutical molecules from crude feedstreams
  • Large pores that result in ultrahigh binding capacities at fast linear velocities
  • Hydrophilic polymeric beads engineered for high mechanical stability and low backpressures
  • Robust polymer designed to withstand repetitive clean-in-place cycles
  • Optimized to operate under 2 bar backpressure at velocities of 1,200 cm/hr
  • Stable to 1 M NaoH exposure to 10,000 hr
  • Availability in biopharmaceutical quantities
  • Fully supported for regulatory submissions

 

UNOsphere S Support #156-0113
UNOsphere S Support

156-0111
25 ml, strong cation exchange chromatography media, 80 μm particle size

List Price:   $88.00
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UNOsphere S Support #156-0115
UNOsphere S Support

156-0113
100 ml, strong cation exchange chromatography media, 80 μm particle size

List Price:   $275.00
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UNOsphere S Support #156-0117
UNOsphere S Support

156-0115
500 ml, strong cation exchange chromatography media, 80 μm particle size

List Price:   $977.00
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UNOsphere S Support

156-0117
10 L, strong cation exchange chromatography media, 80 μm particle size

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UNOsphere Rapid S Media #156-0211
(Discontinued) UNOsphere Rapid S Media

156-0211
25 ml, strong cation exchange chromatography media, 100 μm particle size

This product is no longer available. Please contact technical support for more information on replacement product.
UNOsphere Rapid S Media #156-0213
(Discontinued) UNOsphere Rapid S Media

156-0213
100 ml, strong cation exchange chromatography media, 100 μm particle size

This product is no longer available. Please contact technical support for more information on replacement product.
UNOsphere Rapid S Media #156-0215
(Discontinued) UNOsphere Rapid S Media

156-0215
500 ml, strong cation exchange chromatography media, 100 μm particle size

This product is no longer available. Please contact technical support for more information on replacement product.
UNOsphere Rapid S Media #156-0217
(Discontinued) UNOsphere Rapid S Media

156-0217
10 L, strong cation exchange chromatography media, 100 μm particle size

This product is no longer available. Please contact technical support for more information on replacement product.
Number Description Options
2780 Purification of Murine IgG1 Using UNOsphere S and CHT Ceramic Hydroxyapatite Chromatography, Rev A Click to download [ Add to Cart (Free) ]
2774 Purification of Transgenic Antibody From Corn Seed Using UNOsphere S and CHT Ceramic Hydroxyapatite Supports, Rev A Click to download
2678 UNOsphere™ S Support Technical Data, Rev C Click to download [ Add to Cart (Free) ]
2669 UNOsphere S Cation Exchange Media Product Information Sheet, Rev C Click to download
5734 UNOsphere Rapid S Media Product Information Sheet, Rev B Click to download [ Add to Cart (Free) ]
4110109 Instruction Manual, UNOsphere Q and S Ion Exchange Media, Rev C Click to download
10010339 Instruction Manual, UNOsphere Rapid S Media, Rev A Click to download