UNOsphere Q Media

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Overview

UNOsphere ion exchange media are designed to meet the increasing needs of the biopharmaceutical industry for high-productivity chromatographic media.

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Description

Description

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UNOsphere ion exchange media are designed to meet the increasing needs of the biopharmaceutical industry for high-productivity chromatographic media. UNOsphere, a hydrophilic polymeric support based on a single-step polymerization process, delivers high productivity through high binding capacity and low backpressures. The single-step polymerization process produces a support unsurpassed in quality and batch-to-batch reproducibility. UNOsphere Q media are strong anion exchangers.

Features include:

  • Optimized to operate under 2 bar backpressure at linear velocities of 1,200 cm/hr
  • Stable to 1 M NaOH exposure up to 10,000 hours
  • Efficient capture of biomolecules from crude feedstreams
  • Large pores that result in ultrahigh binding capacities at fast linear velocities
  • Hydrophilic polymeric beads
  • Robust polymer designed to withstand repeated clean-in-place cycles
  • Biopharmaceutical manufacturing quantities available
  • Fully supported for regulatory submission

Most production chromatography systems have maximum pressure limits of 3 bar. The median particle size of UNOsphere Q support is 120 µm, which generates a backpressure of less than 2 bar at 1,200 cm/hr. The highly macroporous nature of UNOsphere Q support provides high binding capacities that range from 180 to 125 mg BSA per ml in the linear velocity range of 150–1,200 cm/hr, respectively. Harsh clean-in-place and sanitization cycles will not affect the long-term stability or performance of UNOsphere Q media.

Regulatory support files are available upon request. Bio-Rad Laboratories is an ISO 9001-registered corporation.

Specifications

Specifications

Type of ion exchanger
Strong anion
Functional group
-N(Ch3)3+
Total ionic capacity
120 µeq/m
Dynamic binding capacity*
 
     at 150 cm/hr
180 mg BSA/ml
     at 600 cm/hr
125 mg BSA/ml
Median particle size
120 µm
Recommended linear flow rate range**
50–1,200 cm/hr
Chemical stability
 
     in 1.0 M NaOH (20°C)
Up to 10,000 hr
     in 1.0 M HCl (20°C)
Up to 200 hr
pH stability range
pH 1–14
Regeneration conditions
1–2 M NaCl
Storage conditions
20% ethanol
0.1 M NaOH for 30 days
* 10% breakthrough capacity determined with 2.0 mg/ml BSA in a 1.1 x 10 cm column.
 
** UNosphere Q support packed into a 20 cm bed height and run at 1,200 cm/hr generates less than 2 bar backpressure.
 

Ordering

Ordering

items

Use the filters below to refine results!

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7324820
1 row of 8 columns, 600 μl, RoboColumn unit prepacked with UNOsphere Q strong anion exchange chromatography media
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7324714
2 x 96-well plates, 20 μl, filter plate prepacked with UNOsphere Q Strong Anion Exchange Chromatography Media
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7324819
1 row of 8 columns, 200 μl, RoboColumn unit prepacked with UNOsphere Q Strong Anion Exchange Chromatography Media
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7324820
1 row of 8 columns, 600 μl, RoboColumn unit prepacked with UNOsphere Q strong anion exchange chromatography media

Kits & Reagents

Kits & Reagents

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7324650
Includes one 1 ml cartridge each of UNOsphere™ S, UNOsphere Q, Macro-Prep® High Q, Macro-Prep High S, Macro-Prep DEAE
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7324408
UNOsphere SUPrA™ affinity cartridge, UNOsphere™ Q cartridge, CHT™ Type 1, 40 µm cartridge, 1 x 5 ml each
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7324100
Pkg of 5, 1 ml, prepacked ion exchange column, 40 mm x 5.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system
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7324102
Pkg of 1, 5 ml, prepacked ion exchange column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system
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7324104
Pkg of 5, 5 ml, prepacked ion exchange column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system
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(Discontinued) Media Sampler Pack
1580100
Includes 25 ml each of UNOsphere™ Q and UNOsphere S, Macro-Prep® DEAE, Macro-Prep High Q, and Macro-Prep High S; 5 ml of UNOsphere SUPrA™; 10 g each of CHT™ Ceramic Hydroxyapatite Types I and II, 40 μm; and 10 g of CFT™ Ceramic Fluoroapatite Type II, 40 μm
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1250561
Pkg of 6 vials, lyophilized mixture of equine myoglobin, conalbumin, chicken ovalbumin, soybean trypsin inhibitor, for ion exchange chromatography

Documents

Documents

Number Description Options
4110109
Instruction Manual, UNOsphere Q and S Ion Exchange Media, Rev C
2729
UNOsphere™ Q Support Technical Data, Rev A
10005454
Instruction Manual, Bio-Scale™ Mini UNOsphere™ Q, S and Rapid S Cartridges, 1 and 5 ml, Rev E
6862
Monoclonal Antibody Purification: Intermediate Purification Resins Brochure, Ver A
6983
Native and Recombinant Protein Purification Resins/Media Selection Card
6786
Monoclonal Antibody Purification Resin Selection Card, Ver A
6789
IgG Polyclonal Antibody Purification Resin Selection Card, Ver A
6791
Virus Purification Resin Selection Card, Ver A
5543
Effective Cleaning and Sanitizing of Anion Exchange Chromatography Resins, Rev A
6875
Practical Guide: Selecting the Optimal Resins for Monoclonal Antibody Process Purification, Ver A
6810
Practical Guide: Selecting the Optimal Resins for the Process Purification of Native and Recombinant Proteins, Ver A
6813
Practical Guide: Selecting the Optimal Resins for Endotoxin Depletion in Process Purification, Ver A
6790
Practical Guide: Selecting the Optimal Resins for Mammalian Virus Process Purification and Clearance, Ver A
6881
Practical Guide: Selecting the Optimal Resins for Removal of DNA Contamination during Process Purification, Ver A
6719
Development of an Efficient Manufacturing Process for Adenovirus, Ver A
6889
Novel Selectivities and Workflows to Streamline mAb Purification Processes, Ver A
6867
UNOsphere™ Chromatography Resins Publications List, Ver A
6886
Quick Monoclonal Antibody Purification Strategy: Affinity-Based Capture Flier, Ver A
5662
Process Chromatography Applications Laboratory Brochure, Rev B