UNOsphere S Media

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Overview

UNOsphere S cation exchange media are designed to meet the increasing needs of the biopharmaceutical industry for high-productivity chromatographic media.
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Description

Description

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UNOsphere S cation exchange media are designed to meet the increasing needs of the biopharmaceutical industry for high-productivity chromatographic media. UNOsphere, a hydrophilic polymeric support based on a single-step polymerization process, delivers high productivity through high binding capacity and low backpressures. The single-step polymerization process produces a support unsurpassed in quality and batch-to-batch reproducibility.

UNOsphere S support is a strong cation exchanger.

Features include:

  • Optimized to operate under 2 bar backpressure at linear velocities of 1,200 cm/hr
  • Stable to 1 M NaOH exposure up to 10,000 hours
  • Efficient capture of biomolecules from crude feedstreams
  • Large pores that result in ultrahigh binding capacities at fast linear velocities
  • Hydrophilic polymeric beads
  • Robust polymer designed to withstand repeated clean-in-place cycles
  • Biopharmaceutical manufacturing quantities available
  • Fully supported for regulatory submission

Most production chromatography systems have maximum pressure limits of 3 bar. The median particle size of UNOsphere S media is 80 µm, which generates a backpressure of less than 2 bar at 1,200 cm/hr. The highly macroporous nature of UNOsphere S media provides high binding capacities of 60 mg human IgG per ml of media at 150 ml/min. Harsh clean-in-place and sanitization cycles will not affect the long-term stability of UNOsphere media.

Regulatory support files are available upon request. Bio-Rad Laboratories is an ISO 9001-registered corporation.

Specifications

Specifications

Type of ion exchanger
Strong cation
Functional group
-SO3-
Total ionic capacity
280 ± 30 µeq/ml
Dynamic binding capacity
150 cm/hr: 60 mg IgG/ml
1,200 cm/hr: 30 mg IgG/ml
(10% breakthrough capacity determined with 4.5 mg/ml human IgG)
Median particle size
80 µm
Recommended maximum linear flow rate
50–1,200 cm/hr
UNOsphere S support packed into a 20 cm bed height and run at 1,200 cm/hr generates less than 2 bar backpressure.
Chemical stability
 
1.0 M NaOH (20°C)
Up to 2,000 hr
1.0 M HCl (20°C)
Up to 200 hr
pH stability range
pH 1–14
Regeneration conditions
1–2 M NaCl
Storage conditions
20% Ethanol
0.1 N NaOH for 30 days

Ordering

Ordering

items

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7324710
2 x 96-well plates, 20 μl, filter plate prepacked with UNOsphere S Strong Cation Exchange Chromatography Media
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7324813
1 row of 8 columns, 200 μl, RoboColumn unit prepacked with UNOsphere S Strong Cation Exchange Chromatography Media
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7324814
1 row of 8 columns, 600 μl, RoboColumn unit prepacked with UNOsphere S Strong Cation Exchange Chromatography Media

Kits & Reagents

Kits & Reagents

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7324650
Includes one 1 ml cartridge each of UNOsphere™ S, UNOsphere Q, Macro-Prep® High Q, Macro-Prep High S, Macro-Prep DEAE
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7324110
Pkg of 5, 1 ml, prepacked ion exchange column, 40 mm x 5.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system
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7324112
Pkg of 1, 5 ml, prepacked ion exchange column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system
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7324114
Pkg of 5, 5 ml, prepacked ion exchange column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system
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(Discontinued) Media Sampler Pack
1580100
Includes 25 ml each of UNOsphere™ Q and UNOsphere S, Macro-Prep® DEAE, Macro-Prep High Q, and Macro-Prep High S; 5 ml of UNOsphere SUPrA™; 10 g each of CHT™ Ceramic Hydroxyapatite Types I and II, 40 μm; and 10 g of CFT™ Ceramic Fluoroapatite Type II, 40 μm
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1250562
Pkg of 6 vials, lyophilized mixture of equine myoglobin, Ribonuclease A, cytochrome c, for use in ion exchange chromatography

Documents

Documents

Number Description Options
4110109
Instruction Manual, UNOsphere Q and S Ion Exchange Media, Rev C
2669
UNOsphere™ S Cation Exchange Resin Product Information Sheet, Ver D
7026
Enhanced Purification of Similarly Charged Proteins with Self-Generated On-Column Resin pH Gradients, Rev A
5515
Guidelines for Using UNOsphere S Cation Exchange Support, Rev A
10005454
Instruction Manual, Bio-Scale™ Mini UNOsphere™ Q, S and Rapid S Cartridges, 1 and 5 ml, Rev E
6983
Native and Recombinant Protein Purification Resins/Media Selection Card
6789
IgG Polyclonal Antibody Purification Resin Selection Card, Ver A
6875
Practical Guide: Selecting the Optimal Resins for Monoclonal Antibody Process Purification, Ver A
6810
Practical Guide: Selecting the Optimal Resins for the Process Purification of Native and Recombinant Proteins, Ver A
6790
Practical Guide: Selecting the Optimal Resins for Mammalian Virus Process Purification and Clearance, Ver A
6793
Practical Guide: Selecting the Optimal Resins for Immunoglobulin G (IgG) Process Purification, Ver A
6719
Development of an Efficient Manufacturing Process for Adenovirus, Ver A
6889
Novel Selectivities and Workflows to Streamline mAb Purification Processes, Ver A
6867
UNOsphere™ Chromatography Resins Publications List, Ver A
5662
Process Chromatography Applications Laboratory Brochure, Rev B