CFT Ceramic Fluoroapatite Resin

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Overview

CFT Ceramic Fluoroapatite (Ca10(PO4)6F2) mixed-mode media is a rigid, spherical macroporous support used in the purification of biologically active compounds.
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Description

Description

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CFT Ceramic Fluoroapatite, Ca10(PO4)6F2, is a rigid, spherical macroporous support used in the purification of biologically active compounds. CFT Ceramic Fluoroapatite Resin is a composite of fluoroapatite and hydroxyapatite prepared by chemically converting hydroxyapatite nanocrystals to fluoroapatite with a fluorine reagent.

CFT Resin can be used under stringent chromatography conditions to separate acidic proteins requiring buffered conditions as low as pH 5.6 with minimal effect on the solubility or lifetime of the support.

CFT Resin has a high binding capacity and may be used reproducibly over an extended number of chromatography runs. Its tensile strength, chemical durability, and density provide excellent throughput and consistent performance for all separations, including biopharmaceutical process-scale manufacturing.

Features and Benefits

  • High-density particles for fast, simple column packing
  • Sintering at 700°C (Type II) for heavy-duty, durable supports
  • Rigid particles for fast cleaning and equilibration
  • Inorganic calcium phosphate for distinct selectivities

Regulatory support files are available upon request. Bio-Rad Laboratories is an ISO 9001-registered corporation.

Specifications

Specifications

Functional groups
Ca2+, PO4 3-, F-
Nominal mean particle size
40 ± 4 µm
Nominal pore diameter range
600–800 A
Packing density (g/ml packed bed)
.86 g/ml
Dynamic binding capacity
14–21.5 mg lysozyme/g
Typical IgG binding capacity
33 mg/ml
Maximum operating pressure
55 bar (800 psi)
Operating pH range
5–14
Regeneration
Normal conditions: 400 mM sodium phosphate, pH 7.4; Difficult conditions: 400–1,000 mM sodium phosphate, pH 11–12
Sanitation
1–2 M NaOH or KOH
Storage pH range
11–14

Ordering

Ordering

items

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1585200
10 g, fluoroapatite and hydroxyapatite composite, CFT ceramic fluoroapatite multimodal chromatography media, Type II, 40 μm particle size
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1575000
100 g, fluoroapatite and hydroxyapatite composite, CFT ceramic fluoroapatite multimodal chromatography media, Type II, 40 μm particle size
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157-5100
1 kg, fluoroapatite and hydroxyapatite composite, CFT ceramic fluoroapatite multimodal chromatography media, Type II, 40 μm particle size
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157-5500
5 kg, fluoroapatite and hydroxyapatite composite, CFT ceramic fluoroapatite multimodal chromatography media, Type II, 40 μm particle size

Accessories

Accessories

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7320113
Low-pressure system fittings kit, includes 1/4–28 female to male and 1/4–28 female to female luers to connect 1 cartridge to BioLogic DuoFlow™ and NGC Systems
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7320112
Low-pressure adaptor fittings kit, includes luer to 10-32 fittings to connect 1 cartridge to an HPLC system
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7321011
Pkg of 500, 1.5 x 12 cm, polypropylene columns, 14 cm, 20 ml bed volume, empty, includes upper bed supports, end caps, tip closures

Kits & Reagents

Kits & Reagents

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7324405
5 ml, prepacked, CFT ceramic fluoroapatite multimodal chromatography media cartridge, Type II
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7324406
Pkg of 5, 5 ml, prepacked, CFT ceramic fluoroapatite multimodal chromatography media cartridge, Type II
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(Discontinued) Media Sampler Pack
1580100
Includes 25 ml each of UNOsphere™ Q and UNOsphere S, Macro-Prep® DEAE, Macro-Prep High Q, and Macro-Prep High S; 5 ml of UNOsphere SUPrA™; 10 g each of CHT™ Ceramic Hydroxyapatite Types I and II, 40 μm; and 10 g of CFT™ Ceramic Fluoroapatite Type II, 40 μm

Documents

Documents

Number Description Options
3111
CFT Ceramic Fluoroapatite Product Information Sheet, Rev B
5584
Bio-Scale Mini Cartridges, Product Information Sheet, Rev B
6982
Mixed-Mode Resins/Media Selection Guide
[ Add to Cart (Free) ]
6789
IgG Polyclonal Antibody Purification Resin Selection Card, Ver A
6791
Virus Purification Resin Selection Card, Ver A
6793
Practical Guide: Selecting the Optimal Resins for Immunoglobulin G (IgG) Process Purification, Ver A
6813
Practical Guide: Selecting the Optimal Resins for Endotoxin Depletion in Process Purification, Ver A
6810
Practical Guide: Selecting the Optimal Resins for the Process Purification of Native and Recombinant Proteins, Ver A
6549
Mammalian Virus Purification Using Ceramic Hydroxyapatite, Rev A
[ Add to Cart (Free) ]
5853
Chimeric IgG Monoclonal Antibody Purification: A Comparative Study Using CHT Ceramic Hydroxyapatite and CFT Ceramic Fluoroapatite Chromatographic Media, Rev A
5913
Separation of Fab and Fc Fragments from Monoclonal Antibody Papain Digest on Ceramic Hydroxyapatite and Ceramic Fluoroapatite, Rev A
6870
Ceramic Apatite-Based Chromatography Media Publications List, Ver A
5662
Process Chromatography Applications Laboratory Brochure, Rev B
4110183
Instruction Manual, CFT Ceramic Fluoroapatite