Macro-Prep High Q and High Q-3HT Resins

Macro-Prep High Q and High Q-3HT Resins

Overview

Macro-Prep High Q strong anion exchange support contains quaternary amine functional groups and is ideal for purifying acidic and neutral proteins and peptides.

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Description

Description

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Macro-Prep High Q Resin is a strong anion exchange resin containing quaternary amine functional groups that is ideal for purifying acidic and neutral proteins and peptides. It is an excellent choice for rapid purification and meets the demands of analytical, semipreparative, and process-scale applications.

The physical structure of Macro-Prep High Q Resin is specifically designed to achieve excellent resolution at very high flow rates. It consists of rigid methacrylate beads that have minimal shrinkage and swelling tendencies, enabling their effective use in low- and medium-pressure chromatography.

It is also available in a different composition – Macro-Prep High Q-3HT. The 3HT product uses a base bead which is polymerized faster resulting in a slightly larger pore size and a different ligand density range.

The superior mechanical and chemical stability of Macro-Prep High Q Resins make it a preferred choice over other Q supports on the market. Macro-Prep High Q Resins can be sanitized with chaotropic agents and can be autoclaved. They provide consistent and reproducible results.

Features and Benefits

  • High capacity for biomolecules
  • High resolution of complex biological mixtures
  • Rigid polymer matrix that allows high flow rates at moderate pressures
  • Hydrophilic matrix that reduces nonspecific binding
  • Macroporous beads that enhance access to ionic sites
  • High chemical, mechanical, and thermal stability

More Information

Regulatory support files are available upon request. Bio-Rad Laboratories is an ISO 9001-registered corporation.

Specifications

Specifications

Properties of Macro-Prep High Q Support
 
Type of support
Strong anion exchanger
Functional group
–N+(CH3)3
Typical dynamic binding capacity
≥37 BSA/ml
Median particle size
50 µm
Nominal pore diameter
1,000 Å
Recommended maximum linear flow rate
600 cm/hr
pH stability
1–10
Regeneration
in 1–2 M NaCl/KCl or 70% ethanol
Chemical Compatibility of Macro-Prep High Q Support
 
1% SDS
Yes
8 M guanidine-HCI
Yes
1 N HCI
Yes
100% ethanol
Yes

Ordering

Ordering

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Use the filters below to refine results!

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1560042 tn
156-0042

5 L, strong anion exchange chromatography media, for analytical, semi-preparative, and process scale applications, 50 μm particle size

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12003681 tn
12003681

5 L, strong anion exchange chromatography media, for analytical, semi-preparative, and process scale applications, 50 μm particle size, shipped in 2% benzyl alcohol

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1560043 tn
156-0043

10 L, strong anion exchange chromatography media, for analytical, semi-preparative, and process scale applications, 50 μm particle size

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1560043 tn
12003682

10 L, strong anion exchange chromatography media, for analytical, semi-preparative, and process scale applications, 50 μm particle size, shipped in 2% benzyl alcohol

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EconoFit_Columns-1x1ml-Sku_TN.png
12009275

Pkg of 1, 1 ml, prepacked anion exchange column, 7 mm x 25 mm, max. pressure 72 psi, for use with NGC Systems or any chromatography system

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EconoFit_Columns-5x1ml-Sku_TN.png
12009267

Pkg of 5, 1 ml, prepacked anion exchange column, 7 mm x 25 mm, max. pressure 72 psi, for use with NGC Systems or any chromatography system

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EconoFit_Columns-1x5ml-Sku_TN.png
12009268

Pkg of 1, 5 ml, prepacked anion exchange column, 16 mm x 25 mm, max. pressure 72 psi, for use with NGC Systems or any chromatography system

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EconoFit_Columns-5x5ml-Sku_TN.png
12009269

Pkg of 5, 5 ml, prepacked anion exchange column, 16 mm x 25 mm, max. pressure 72 psi, for use with NGC Systems or any chromatography system

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158-0100_tn.jpg
(Discontinued) Media Sampler Pack
1580100
Includes 25 ml each of UNOsphere™ Q and UNOsphere S, Macro-Prep® DEAE, Macro-Prep High Q, and Macro-Prep High S; 5 ml of UNOsphere SUPrA™; 10 g each of CHT™ Ceramic Hydroxyapatite Types I and II, 40 μm; and 10 g of CFT™ Ceramic Fluoroapatite Type II, 40 μm
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1250561
Pkg of 6 vials, lyophilized mixture of equine myoglobin, conalbumin, chicken ovalbumin, soybean trypsin inhibitor, for ion exchange chromatography

Documents

Documents

Number Description Options
LIT271
Instruction Manual, Macro-Prep Ion Exchange Supports, Rev D
5644
Macro-Prep® High Q Support Bulletin, Rev A
6983
Native and Recombinant Protein Purification Resins/Media Selection Card
6810
Practical Guide: Selecting the Optimal Resins for the Process Purification of Native and Recombinant Proteins, Ver A
6813
Practical Guide: Selecting the Optimal Resins for Endotoxin Depletion in Process Purification, Ver A
1913
Developing and Scaling Up a Purification Procedure With Two New Ion Exchange Supports (Poster), Rev B
1985
Clearance of Murine Leukemia Virus From a Chimeric Monoclonal Antibody Using Ion Exchange Chromatography, Rev B.
2204
Effective Removal of Negatively Charged Interfering Molecules From Proteins, Rev B
6868
Macro-Prep® Chromatography Resins Publications List, Ver A
5662
Process Chromatography Applications Laboratory Brochure, Rev B
6530
Protein Purification Workflow Development Using the Bio-Rad NGC Chromatography System and Stain-Free Gel Technology Application Note
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10000105041
Instruction Manual, EconoFit Macro-Prep High Q, High S, DEAE and CM Columns