The effect of flow rate on the dynamic binding capacity of the Macro-Prep DEAE support. A sample containing 3 mg/ml BSA was separated on a 1 x 10 cm (3 ml) Macro-Prep DEAE column. Sample was loaded onto the column in 10 mM Tris-HCl, pH 7.6 (buffer A) and was eluted in 1 M NaCl in buffer A. Sample was applied until the eluting buffer had an A280 value 50% of the incoming sample.
The effect of flow rate on separation and peak symmetry. A 5 ml sample containing 3.65 mg/ml each myoglobin, conalbumin, BSA, and soybean trypsin inhibitor was loaded onto a column containing 6.56 ml Macro-Prep DEAE support. The sample was loaded in 50 mM Tris-HCl, pH 7.6 (buffer A) and eluted in a gradient of 0–1 M NaCl in buffer A as follows: buffer A for 15 ml, 0–0.35 M NaCl in 85 ml, 0.35–0.65 M NaCl in 15 ml, 0.65–1 M NaCl in 15 ml.
Sample separation with the Macro-Prep DEAE support and two other commercially available DEAE supports. A 100 µl sample containing 9 mg/ml each of myoglobin, conalbumin, ovalbumin, BSA, and soybean trypsin inhibitor was loaded onto a 0.5 cm ID column containing 2 ml of support. Sample was loaded onto the column in 50 mM Tris-HCl, pH 7.6 (buffer A) and was eluted with 1 M NaCl in buffer A. The flow rate was 153 cm/hr (0.5 ml/min). Scales offset for comparison.
Scalability of separations on Macro-Prep DEAE support. Separation of a yeast enzyme concentrate (Sigma) under various conditions of scale. A, a 50 mg sample was separated on a 7.4 ml column (10 mm ID) using a flow rate of 78 cm/hr (1 ml/min) in an analytical flow cell; B, a 500 mg sample was separated on a 64 ml column (25 mm ID) using a flow rate of 78 cm/hr (6.4 ml/min) in an analytical flow cell; C, a 1 g sample was separated on a 64 ml column (25 mm ID) using a flow rate of 305 cm/hr (25 ml/min) in a preparative flow cell. For all separations, sample was loaded in 50 mM Tris-HCl, pH 8.3, washed with 1 column volume of the same buffer, and eluted in a linear gradient of 0–0.35 M NaCl in the same buffer for 22.5 column volumes.
Macro-Prep DEAE Weak Anion Exchange Resins were developed using a macroporous methacrylate polymer and meet the demands of analytical, semipreparative, and process-scale applications.
Read full description
This product contains electrical or electronic material. Learn more about Bio-Rad's EU Recycle Program
Call us at 1-800-4-BIORAD (1-800-424-6723)
When using our online Certificate of Analysis search tool, keep the following in mind:
If you cannot find the Certificate of Analysis you are looking for, please Contact Us.
The catalog number, SKU number, or product number is printed on your product label. See the sample label below for the location of this information.
The lot number or control number (one or the other, not both) is printed on your product label. See the sample label below for the location of this information.
Certificates of Analysis are associated not only with a product, but also with a specific lot of that product. For a given product, there may be several Certificates of Analysis, especially if it is an older product line and has had several lots produced over the years. Using the Certificate of Analysis search tool, you can enter the catalog number and lot/control number for the specific product you have on hand and download the exact Certificate of Analysis you need.
You can use the batch number in place of the lot/control number. Use the Certificate of Analysis search tool, entering the catalog number as usual and the batch number where the lot/control number would go.
Yes. While we do periodically remove Certificates of Analysis for site maintenance purposes, we typically keep them available well beyond a product's expiration date.
There are Certificates of Analysis that correspond to products that are either discontinued or simply not available on the website. In these situations, the Certificate of Analysis is available for download, but other product details, such as the product name, are not.
In this case, you have entered a valid catalog number and lot/control number and we have located the Certificate of Analysis. However, for some reason, the actual file itself is unavailable.
Please use the Request Form here or contact your local Bio-Rad Representative so that we can send you the Certificate of Analysis.
Please reenter your email address in the correct format.
Please enter your email address.
Your subscription information already exists, we will send you an email with specific instructions to manage your existing subscription profile.
To receive the latest news, promotions, and more, sign up for Bio-Rad updates by entering your email address below. You can elect to receive only the types of Bio-Rad communications that are of interest to you..
Home |
Trademarks |
Site Terms |
Terms & Conditions |
Privacy
© 2021 Bio-Rad Laboratories, Inc. United States