Macro-Prep DEAE Resin

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Macro-Prep DEAE Resin

Overview

Macro-Prep DEAE Dynamic Binding Capacity

The effect of flow rate on the dynamic binding capacity of the Macro-Prep DEAE support. A sample containing 3 mg/ml BSA was separated on a 1 x 10 cm (3 ml) Macro-Prep DEAE column. Sample was loaded onto the column in 10 mM Tris-HCl, pH 7.6 (buffer A) and was eluted in 1 M NaCl in buffer A. Sample was applied until the eluting buffer had an A280 value 50% of the incoming sample.

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Macro-Prep® DEAE Support: Effects of Flow Rate

The effect of flow rate on separation and peak symmetry. A 5 ml sample containing 3.65 mg/ml each myoglobin, conalbumin, BSA, and soybean trypsin inhibitor was loaded onto a column containing 6.56 ml Macro-Prep DEAE support. The sample was loaded in 50 mM Tris-HCl, pH 7.6 (buffer A) and eluted in a gradient of 0–1 M NaCl in buffer A as follows: buffer A for 15 ml, 0–0.35 M NaCl in 85 ml, 0.35–0.65 M NaCl in 15 ml, 0.65–1 M NaCl in 15 ml.

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Comparing Purifications of Different DEAE Supports

Sample separation with the Macro-Prep DEAE support and two other commercially available DEAE supports. A 100 µl sample containing 9 mg/ml each of myoglobin, conalbumin, ovalbumin, BSA, and soybean trypsin inhibitor was loaded onto a 0.5 cm ID column containing 2 ml of support. Sample was loaded onto the column in 50 mM Tris-HCl, pH 7.6 (buffer A) and was eluted with 1 M NaCl in buffer A. The flow rate was 153 cm/hr (0.5 ml/min). Scales offset for comparison.

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Macro-Prep DEAE Support: Scaling Up Purification Process

Scalability of separations on Macro-Prep DEAE support. Separation of a yeast enzyme concentrate (Sigma) under various conditions of scale. A, a 50 mg sample was separated on a 7.4 ml column (10 mm ID) using a flow rate of 78 cm/hr (1 ml/min) in an analytical flow cell; B, a 500 mg sample was separated on a 64 ml column (25 mm ID) using a flow rate of 78 cm/hr (6.4 ml/min) in an analytical flow cell; C, a 1 g sample was separated on a 64 ml column (25 mm ID) using a flow rate of 305 cm/hr (25 ml/min) in a preparative flow cell. For all separations, sample was loaded in 50 mM Tris-HCl, pH 8.3, washed with 1 column volume of the same buffer, and eluted in a linear gradient of 0–0.35 M NaCl in the same buffer for 22.5 column volumes.

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Macro-Prep DEAE Weak Anion Exchange Resins were developed using a macroporous methacrylate polymer and meet the demands of analytical, semipreparative, and process-scale applications.
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Description

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Macro-Prep DEAE Resin is a weak anion exchange resin that was developed using a macroporous methacrylate polymer and meet the demands of analytical, semipreparative, and process-scale applications.

This rigid, macroporous, hydrophilic 50 µm support provides exceptional capacity, resolution, and throughput in a chemically and mechanically stable form. Macro-Prep DEAE Resin operates well at low and medium pressures and exhibits minimal volume changes in response to variations in pH or ionic strength. Macro-Prep DEAE Resin provides consistent and reproducible results.

Features and Benefits

  • High capacity for biomolecules
  • High resolution of complex biological mixtures
  • Rigid polymer matrix that allows high flow rates at moderate pressures
  • Hydrophilic matrix that reduces nonspecific binding
  • Macroporous beads that enhance access to ionic sites
  • High chemical, mechanical, and thermal stability

More Information

Regulatory support files are available upon request. Bio-Rad Laboratories is an ISO 9001-registered corporation.


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Macro-Prep DEAE Support

1580020
25 ml, weak anion ion exchange chromatography media, 50 μm particle size

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$57.00 
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Macro-Prep DEAE Support

1560020
100 ml, weak anion exchange chromatography media, 50 μm particle size

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$183.00 
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Macro-Prep DEAE Support

1560021
500 ml, weak anion exchange chromatography media, 50 μm particle size

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$665.00 
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Macro-Prep DEAE Support

156-0022
5 L, weak anion exchange chromatography media, for analytical, semi-preparative, and process scale applications, 50 μm particle size

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Macro-Prep DEAE Support

156-0023
10 L, weak anion exchange chromatography media, for analytical, semi-preparative, and process scale applications, 50 μm particle size

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Bio-Scale™ Mini Ion Exchange Sample Pack

7324650
Includes one 1 ml cartridge each of UNOsphere™ S, UNOsphere Q, Macro-Prep® High Q, Macro-Prep High S, Macro-Prep DEAE

This product is being discontinued soon. Please contact customer service for more information.
Bio-Scale Mini Macro-Prep DEAE Cartridges

7324140
Pkg of 5, 1 ml, prepacked ion exchange column, 40 mm x 5.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system

This product is being discontinued soon. Please contact customer service for more information.

The following replacement is recommended:

  • EconoFit Macro-Prep DEAE Columns, 5 x 1 ml (12009264)
Bio-Scale Mini Macro-Prep DEAE Cartridge

7324142
Pkg of 1, 5 ml, prepacked ion exchange column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system

This product is being discontinued soon. Please contact customer service for more information.

The following replacement is recommended:

  • EconoFit Macro-Prep DEAE Column, 1 x 5 ml (12009265)
Bio-Scale Mini Macro-Prep DEAE Cartridges

7324144
Pkg of 5, 5 ml, prepacked ion exchange column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic systems or any chromatography system

This product is being discontinued soon. Please contact customer service for more information.

The following replacement is recommended:

  • EconoFit Macro-Prep DEAE Columns, 5 x 5 ml (12009266)
Protein Standard for Anion Exchange Chromatography

1250561
Pkg of 6 vials, lyophilized mixture of equine myoglobin, conalbumin, chicken ovalbumin, soybean trypsin inhibitor, for ion exchange chromatography

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$327.00 
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Documents

Number Description Options
LIT271 Instruction Manual, Macro-Prep Ion Exchange Supports, Rev D Click to download
1942 Macro-Prep DEAE Support for Chromatography of Biomolecules, Rev C. Click to download
6983 Native and Recombinant Protein Purification Resins/Media Selection Card Click to download
6789 IgG Polyclonal Antibody Purification Resin Selection Card, Ver A Click to download
6810 Practical Guide: Selecting the Optimal Resins for the Process Purification of Native and Recombinant Proteins, Ver A Click to download
6793 Practical Guide: Selecting the Optimal Resins for Immunoglobulin G (IgG) Process Purification, Ver A Click to download
2524 Purification of Horse IgGT Using Macro-Prep DEAE and CHT Ceramic Hydroxyapatite Type I Supports, Rev C Click to download
6868 Macro-Prep® Chromatography Resins Publications List, Ver A Click to download
5662 Process Chromatography Applications Laboratory Brochure, Rev B Click to download

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