Chelex® 100 Resin

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Chelex<sup>&reg;</sup> 100 Resin

Overview

Analytical Grade Resin Selection Guide
Analytical Grade Resin Selection Guide
Change in Structure of Chelex Resin with Increasing pH

Chelex chelating resin is efficiently regenerated in dilute acid and operates in basic, neutral, and weakly acidic solutions of pH 4 or higher. At very low pH, the resin acts as an anion exchanger. This figure shows the zwitterionic forms of the Chelex resin as a function of pH.

Selectivity for Divalent Cations
Hg+2 1060 Fe+2 0.130
Cu+2
126 Mn+2 0.024
UO+2 5.70 Ba+2 0.016
Ni+2 4.40 Ca+2 0.013
Pb+2 3.88 Sr+2 0.013
Zn+2 1.00 Mg+2 0.009
Co+2 0.615 Na+1 0.0000001
Cd+2 0.390    

The selectivity of Chelex resin for metal cations corresponds to that of iminodiacetic acid. This is a list of selectivity factors for several divalent cations. The selectivity factor is a quantitative measure of the affinity that Chelex resin displays for a particular cation compared to its affinity for a reference cation, in this case Zn+2.

Chelex 100 Sample Applications and Resins
Application Reference
Preparation of metal-free apoenzyme O'Keefe, E. T., Hill, R. L. and Bell, J. E., Biochem., 19, 4954 (1980).
Removal of calcium from sarcoplasmic reticulum vesicles Chiesi, M. and Inesi, G., Biochem. 19, 2912 (1980).
Removal of extraneously bound metal ions from enzymes prior to NMR and ESR studies Barker, R., et al., Biochem. J., 177, 289 (1979).
Batch removal of calcium from whole blood Raymond, F A. and Weinshilboum, R. M., Clin. Chim. Acta, 58, 185 (1975).
Removal of metals from ATP Sontheimer, G. M., et al., Biochem., 26, 2701 (1987).
Removal of metals from buffer, brine, and biological solutions Knapp, G., et al., J. Anal. Atomic Spectrometry, 2, 611 (1987); Laue, T. M., et al., Biochem., 28, 4762 (1989).
Removal of metals from S100b and melittin Baudier, J., et al., Biochem., 26, 2886 (1987).
Purification of dinucleotides Reinhardt, C. G. and Krugh, T. R., Biochem., 17, 4845 (1978).
Removal of metals from cell suspension Bosron, W. F., Kennedy F. S. and Vallee, B. L., Biochem., 14, 2275 (1975).
Batch removal of metals from urine Agarwal, M., Bennett, R. B., Stump, I. G. and D'Avria, J. M., Anal. Chem., 47, 924 (1975).
Removal of metals from enzyme solutions Dunn, M. F., Pattison, S. E., Storm, M. C. and Quiel, E., Biochem., 19, 718 (1980).
Removal of metals from guinea pig complement
Amiraian, K., McKinney, J. A. and Duchna, L., Immunology, 26, 1135 (1974).
Removal of aldehydes and peroxides from polyethylene glycol Ray, W. J. and Pavathingal, J. M., Anal. Biochem., 146, 307 (1985).
Removal of interfering components from myo-inositol Brandt, S. J., Dougherty, R. W., Lapetina, E . G. and Niedel, J. E., Proc. Nat. Acad. Sci., 82, 3277 (1985).
Calcium removal from protein kinases Putnam-Evans, C. L., Harmon, A. C. and Cormier, M. J., Biochem., 29, 2488 (1990).
Removal of divalent cations from NMR stock solutions Devlin, C. C. and Grisham, C. M., Biochem., 29, 6192 (1990). Chuknyisky, P. P., Rifkind, J. M., Tarien, E., Beal, R. B. and Eichhorn, G. L., Biochem., 29, 5987 (1990).
Removal of paramagnetic impurities from oligonucleotides for NMR analysis Kochoyan, M., Leroy, J.L. and Guéron, M. Biochem., 29, 4799 (1990), Schroeder, S.A., Roongta, V., Fu J. M., Jones, C.R., and Gorenstein, D.G., Biochem., 28, 8292 (1989).
Trace metal removal from buffer solutions and seawater Pai, S.-C., Chen, T.-C., Wong, G.T.F. and Hung, C.- C., Anal. Chem., 62, 774 (1990)
Iron removal from bacteria Trost, J.T. and Blankenship, R.E., Biochem., 28, 9898 (1989)
Trace metal ion removal from buffer solutions Yong, G., Leone,C. and Strothkamp, K.G., Biochem., 29, 9684 (1990). Severns, J.C.
and McMillin, D.R., Biochem., 29, 8592 (1990) Shang, S., Liao Y.-D., Wu, F., Y,-H. and Wu, C.- W., Biochem., 28, 9790 (1989), Chung, H.K. and Ingle, J.D., Anal. Chem., 62, 2547 (1990), Grimshaw, C.E., Shahbaz, M. and Putney, C.G., Biochem., 29, 9936 (1990).
Removal or multivalent cation contaminants Swanson, J.E., and Feigenson, G.W., Biochem., 29, 8291 (1990).
Purification of NMR reagents Brito, R.M.M., Rudolph, F.B., and Rosevear, P.R., Biochem., 30, 1461 (1991). Ray, W.J. Burgner, J.W., and Post, C.B., Biochem., 29, 2770 (1990)
Calcium removal from calmodulin Vorherr., T., James, P., Krebs, J., Enyedi, A., McCormick, D.J., Penniston, J.T., and Carafoli, E., Biochem., 29, 355 (1990)
Purification of zinc isotopes from human blood and excrement Gökmen, I.G., Aras, N.K., Gordon, G.E., Wastney, M.E., and Henkin, R.I., Anal. Chem., 61, 2757 (1989)
Calcium removal from buffer solutions Kinoshita, C.M., Ying, S.-C., Hugli, T.E., Siegel, J.N., Potempa., L.A., Jiang, H., Houghten, R.A. and Gewurz, H., Biochem., 28, 9840 (1989), Thielens, N.M., Dorsselaer, A.V., Gagnon, J. and Arlaud, G.J., Biochem., 29, 3570 (1990)
Removal of zinc from buffer solutions Jefferson, J.R., Hunt, J.B., and Ginsburg, A., Biochem., 29, 6687 (1990)
DNA Extraction for PCR Walsh, P.S., Metzger, D.A. and Higuchi, R., BioTechniques, 10, 506 (1991)
Stability of Selected Analytical Grade Resins to Various Treatments
Resin
Heat*
Solvents (Alcohols,
Hydrocarbons, etc.)
Oxidation
Reduction
AG 1 OH- form, fair to 50°C
Cl- and other forms,
good to 150°C
Very good Slowly dissolves in
hot 15% HNO3
or concentrated H2O2
Good**
AG 2 OH- form, fair to 30°C
Cl- form, good to 150°C
Very good Slowly dissolves in
hot 15% HNO3
Good**
AG 50W Good to 150°C Very good Slowly dissolves in
hot 15% HNO3
Very good
AG 4-X4 Good to 60°C Good Good Unknown
AG 501-X8 Good to 50°C Very good Slowly dissolves in
hot 15% HNO3
or concentrated H2O2
Good**
AG 11 A8 Good to 100°C Good Slowly dissolves in
hot 15% HNO3
or concentrated H2O2
Good
Bio-Rex 5 Good to 60°C Good Good Excellent
Bio-Rex 70 Good to 100°C Good Good Good
Bio-Rex MSZ 501(D) Good to 50°C Very good Slowly dissolves in
hot 15% HNO3
or concentrated H2O2
Good**
Chelex 100 Good to 75°C Good Breaks down in strong
oxidizing agents
Unknown
* All anion resins may be autoclaved except the CH- form; all cation exchange resins may be autoclaved.
** AG 1, AG 2, AG 501, and Bio-Rex MSZ 501 (D) resins will slowly break down in the presence of sulfur-containing reducing agents.
AG Resin Wet Mesh and Diameters
Wet mesh (U.S. Standards) 16 20 40 50 80 100 140 200 270 325 400
Diameter, µm 1,180 850 425 300 180 150 106 75 53 45 38
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Chelex 100 resin is a styrene divinylbenzene copolymer containing paired iminodiacetate ions, which act as chelating groups in binding polyvalent metal ions. The carboxylic acid groups of Chelex 100 resin classify it as a weak cation exchange resin, but it differs from other exchangers in this class by featuring uniquely high selectivities for metal ions and much higher bond strengths. The resin can be used to ultrapurify buffers and ionic reagents: it will scavenge metal contaminants without altering the concentration of nonmetal ions.

Chelex 100 chelating ion exchange resin has a stronger selectivity for copper, iron, and other heavy metal ions over monovalent cations such as sodium and potassium. Its selectivity for divalent over monovalent ions is approximately 5,000 to 1, and it has a strong attraction for transition metals, even in highly concentrated salt solutions.

The following forms of Chelex 100 resin are available:

  • Analytical grade Chelex 100 resin — exhaustively sized, purified, and converted to make it suitable for accurate, reproducible analytical techniques
  • Biotechnology grade Chelex 100 resin — the analytical grade form certified to contain fewer than 100 microorganisms per gram of resin
Larger volumes and special packaging for industrial applications are available upon request.

Type Weak cation, chelating
Functional group R-CH2N(CH2COO-)2
Matrix Styrene divinylbenzene
Crosslinkage 1%
Approx. MW exclusion 3,500
Operating stability/pH range 4–14/0–14
Min. wet capacity (meq.ml) 0.4
Nominal density 0.65
Ionic forms Sodium, iron*
* Iron form is glyphosate concentration.  
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Chelex® 100 Chelating Resin, analytical grade, 50–100 mesh, sodium form, 500 g

1422822
500 g, cation exchange resin, sodium form, 1% crosslinkage, 50–100 dry mesh size, 300–1,180 µm wet bead size, ~3,500 MW limit

0 1422822 /templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Media/142-2822_0921120700762.xml 1422822 09/21/09 12:07 PM N en 09/21/19 12:07 PM true true internet/product_SKU AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA LSR 2018-02-01 00:17:44 1422822-chelex-100-chelating-resin-analytical-grade-50-ndash-100-mesh-sodium-form-500-g 1422822 Chelex 100 Resin Chelex<sup>®</sup> 100 Chelating Resin, analytical grade, 50&ndash;100 mesh, sodium form, 500 g 500 g, cation exchange resin, sodium form, 1% crosslinkage, 50–100 dry mesh size, 300–1,180 µm wet bead size, ~3,500 MW limit 500 g, cation exchange resin, sodium form, 1% crosslinkage, 50–100 dry mesh size, 300–1,180 µm wet bead size, ~3,500 MW limit 500 g, cation exchange resin, sodium form, 1% crosslinkage, 50–100 dry mesh size, 300–1,180 µm wet bead size, ~3,500 MW limit /webroot/web/images/lsr/products/chromatography/sku_thumb/global/142-2822_tn.jpg /webroot/web/images/lsr/products/chromatography/sku_view/global/142-2822_view.jpg /webroot/web/images/lsr/products/chromatography/sku_feature/global/142-2822_feature.jpg Chelex<sup>®</sup> 100 Chelating Resin, analytical grade, 50-100 mesh, sodium form, 500 g preview RepEx Chelex 100 Resin 500 g, cation exchange resin, sodium form, 1% crosslinkage, 50–100 dry mesh size, 300–1,180 µm wet bead size, ~3,500 MW limit 142-2822, 1422822, chelex, 100, resin, ion, cation, exchange, iex, chromatography, purification, fplc, media 09/21/09 12:07 PM 09/21/19 12:07 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Chromatography/Chromatography-Media N 0 <p>Choose analytical grade Chelex 100 Resin, a weak cation exchange media, for chelation, DNA extraction for PCR, and other cation exchange protocols. Use this sodium-form resin for gravity or low-pressure chromatography.</p> <ul> <li>Large-mesh resin is effective for preparative column and batch protocols</li> <li>Selectivity for divalent over monovalent ions is approximately 5,000 to 1</li> <li>Strong attraction for transition metals, even in highly concentrated salt solutions</li> <li>Inactivates enzymes, including DNases, that require divalent cations for activity</li> <li>Larger volumes and special packaging are available upon request</li> </ul> <h2 class="descHeader">Product Options</h2> <table class="pd_table pd_gridlines" border="0"> <tbody> <tr> <td width="450" align="center" valign="top"><strong>Resin</strong></td> <td width="250" align="center" valign="top"><strong>Strength</strong></td> <td width="250" align="center" valign="top"><strong>Grade*</strong></td> <td width="150" align="center" valign="top"><strong>Crosslink</strong></td> <td width="325" align="center" valign="top"><strong>Ionic Form</strong></td> <td width="325" align="center" valign="top"><strong>Dry Mesh</strong></td> <td width="450" align="center" valign="top"><strong>Wet Bead Size &micro;m</strong></td> <td width="319" align="center" valign="top"><strong>MW Limit </strong></td> <td width="319" align="center" valign="top"><strong>Catalog Number</strong></td> </tr> <tr class="pd_colorbackground"> <td align="center" valign="top">Chelex 100</td> <td align="center" valign="top">Weak</td> <td align="center" valign="top">Analytical</td> <td align="center" valign="top">1%</td> <td align="center" valign="top">Sodium</td> <td align="center" valign="top">50&ndash;100</td> <td align="center" valign="top">300&ndash;1,180</td> <td align="center" valign="top">~3,500</td> <td align="center" valign="top">1422822</td> </tr> <tr> <td align="center" valign="top">Chelex 100</td> <td align="center" valign="top">Weak</td> <td align="center" valign="top">Analytical</td> <td align="center" valign="top">1%</td> <td align="center" valign="top">Sodium</td> <td align="center" valign="top">100&ndash;200</td> <td align="center" valign="top">150&ndash;300</td> <td align="center" valign="top">~3,500</td> <td align="center" valign="top"><a href="/en-us/sku/1422832-chelex-100-chelating-resin-analytical-grade-100-ndash-200-mesh-sodium-form-500-g?ID=1422832">1422832</a></td> </tr> <tr class="pd_colorbackground"> <td align="center" valign="top">Chelex 100</td> <td align="center" valign="top">Weak</td> <td align="center" valign="top">Biotech</td> <td align="center" valign="top">1%</td> <td align="center" valign="top">Sodium</td> <td align="center" valign="top">100&ndash;200</td> <td align="center" valign="top">150&ndash;300</td> <td align="center" valign="top">~3,500</td> <td align="center" valign="top"><a href="/en-us/sku/1432832-chelex-100-chelating-resin-biotechnology-grade-100-ndash-200-mesh-sodium-form-100-g?ID=1432832">1432832</a></td> </tr> <tr> <td align="center" valign="top">Chelex 100</td> <td align="center" valign="top">Weak</td> <td align="center" valign="top">Analytical</td> <td align="center" valign="top">1%</td> <td align="center" valign="top">Iron</td> <td align="center" valign="top">100&ndash;200</td> <td align="center" valign="top">150&ndash;300</td> <td align="center" valign="top">~3,500</td> <td align="center" valign="top"><a href="/en-us/sku/1422825-chelex-100-chelating-resin-analytical-grade-100-ndash-200-mesh-iron-form-100g?ID=1422825">1422825</a></td> </tr> <tr class="pd_colorbackground"> <td align="center" valign="top">Chelex 100</td> <td align="center" valign="top">Weak</td> <td align="center" valign="top">Analytical</td> <td align="center" valign="top">1%</td> <td align="center" valign="top">Sodium</td> <td align="center" valign="top">200&ndash;400</td> <td align="center" valign="top">75&ndash;150</td> <td align="center" valign="top">~3,500</td> <td align="center" valign="top"><a href="/en-us/sku/1422842-chelex-100-chelating-resin-analytical-grade-200-ndash-400-mesh-sodium-form-500-g?ID=1422842">1422842</a></td> </tr> <tr> <td align="center" valign="top">Chelex 100</td> <td align="center" valign="top">Weak</td> <td align="center" valign="top">Mol Bio</td> <td align="center" valign="top">1%</td> <td align="center" valign="top">Sodium</td> <td align="center" valign="top">200&ndash;400</td> <td align="center" valign="top">75&ndash;150</td> <td align="center" valign="top">~3,500</td> <td align="center" valign="top"><a href="/en-us/sku/1421253-chelex-100-chelating-resin-molecular-biology-grade-200-ndash-400-mesh-sodium-form-50-g?ID=1421253">1421253</a></td> </tr> </tbody> </table> <p class="caption">*Biotechnology grade resin is certified to contain less than 100 microorganisms per gram; molecular-biology-grade resin is certified to be endo- and exonuclease-free and contain no ligase inhibitors.</p> <h2 class="descHeader">Related Products</h2> <ul> <li><a href="/en-us/product/poly-prep-chromatography-columns">Poly-Prep<sup>&reg;</sup> Chromatography Columns</a> hold up to 2 ml resin, polypropylene</li> <li><a href="/en-us/product/econo-pac-chromatography-columns">Econo-Pac<sup>&reg;</sup> Chromatography Columns</a> hold from 1 to 20 ml resin, polypropylene</li> <li><a href="/en-us/product/glass-econo-column-columns?ID=2cfb1c6e-32e8-4c72-b532-dd39013d707d">Glass Econo-Column<sup>&reg;</sup> Columns </a></li> <li><a href="/en-us/product/instagene-matrix">InstaGene&trade; Matrix</a> for nucleic acid extraction</li> </ul> <h2 class="descHeader">Related Categories</h2> <ul> <li>Selection of <a href="/en-us/category/cation-exchange-resin">Cation Exchange Resins</a> with different specificities, capacities, operating pressures and flow rates</li> <li><a href="/en-us/category/chromatography-systems-components-accessories">Chromatography Systems, Components, and Accessories</a></li> <li><a href="/en-us/category/biologic-low-pressure-liquid-chromatography-systems">BioLogic&trade; Low-Pressure Liquid Chromatography Systems </a></li> <li><a href="/en-us/category/chromatography-resins?ID=f6b4c81f-147b-4130-83ae-d24dbba71eb2">Chromatography Resins </a></li> <li><a href="/en-us/category/chromatography-columns?ID=826b06e0-11cf-4f20-a477-e59ebc32eec1">Chromatography Columns </a></li> </ul> <h2 class="descHeader">Supporting Documents</h2> <ul> <li>Chelex 100 and Chelex 20 Chelating Ion Exchange Resin <a href="/webroot/web/pdf/lsr/literature/LIT200.pdf" target="_blank">Instruction Manual</a></li> <li>Chelex 100 Iron-Form Resin for Glyphosate Cleanup <a href="/webroot/web/pdf/lsr/literature/LIT237.pdf" target="_blank">Instruction Sheet</a></li> <li>InstaGene Matrix <a href="/webroot/web/pdf/lsr/literature/Bulletin_2074.pdf" target="_blank">Product Information Sheet</a></li> <li>Lab-Scale Chromatography Sample Preparation <a href="/webroot/web/pdf/lsr/literature/Bulletin_5777A.pdf" target="_blank">Guide</a></li> </ul> <h2 class="descHeader">Online Resources</h2> <ul> <li><a href="/en-us/applications-technologies/introduction-chromatography">Introduction to Chromatography</a></li> <li><a href="/en-us/applications-technologies/pcr-polymerase-chain-reaction">PCR (Polymerase Chain Reaction)</a></li> <li><a href="/en-us/applications-technologies/introduction-digital-pcr">Introduction to Digital PCR</a></li> </ul> <ul> </ul> /webroot/web/html/lsr/products/SKUs/autopopulated Chelex-100-Chelating-Resin-analytical-grade-50-100-mesh-sodium-form-500-g-1422822.html /webroot/web/html/lsr/products/SKUs/autopopulated/Chelex-100-Chelating-Resin-analytical-grade-50-100-mesh-sodium-form-500-g-1422822.html false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1422822&CartQuote=QT&quantity= false true false $856.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1422822&AddToDefaultWishList=true&quantity= true false true true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1422822&quantity= 1422822
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Chelex® 100 Chelating Resin, analytical grade, 100–200 mesh, sodium form, 500 g

1422832
500 g, cation exchange resin, sodium form, 1% crosslinkage, 100–200 dry mesh size, 150–300 µm wet bead size, ~3,500 MW limit

1 1422832 /templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Media/142-2832_0921120700785.xml 1422832 09/21/09 12:07 PM N en 09/21/19 12:07 PM true true internet/product_SKU AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA LSR 2018-02-01 00:17:44 1422832-chelex-100-chelating-resin-analytical-grade-100-ndash-200-mesh-sodium-form-500-g 1422832 Chelex 100 Resin Chelex<sup>®</sup> 100 Chelating Resin, analytical grade, 100&ndash;200 mesh, sodium form, 500 g 500 g, cation exchange resin, sodium form, 1% crosslinkage, 100–200 dry mesh size, 150–300 µm wet bead size, ~3,500 MW limit 500 g, cation exchange resin, sodium form, 1% crosslinkage, 100–200 dry mesh size, 150–300 µm wet bead size, ~3,500 MW limit 500 g, cation exchange resin, sodium form, 1% crosslinkage, 100–200 dry mesh size, 150–300 µm wet bead size, ~3,500 MW limit /webroot/web/images/lsr/products/chromatography/sku_thumb/global/142-2832_tn.jpg /webroot/web/images/lsr/products/chromatography/sku_view/global/142-2832_view.jpg /webroot/web/images/lsr/products/chromatography/sku_feature/global/142-2832_feature.jpg Chelex<sup>®</sup> 100 Chelating Resin, analytical grade, 100-200 mesh, sodium form, 100 g preview RepEx 142-2832 500 g, cation exchange resin, sodium form, 1% crosslinkage, 100–200 dry mesh size, 150–300 µm wet bead size, ~3,500 MW limit 142-2832, 1422832, chelex, 100, resin, ion, cation, exchange, iex, chromatography, purification, fplc, media 09/21/09 12:07 PM 09/21/19 12:07 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Chromatography/Chromatography-Media N 0 <p>Choose analytical grade Chelex 100 Resin, a weak cation exchange media, for chelation, DNA extraction for PCR, and other cation exchange protocols. Use this sodium-form resin for gravity or low-pressure chromatography.</p> <ul> <li>Medium-mesh resin is effective for both preparative and analytical protocols</li> <li>Selectivity for divalent over monovalent ions is approximately 5,000 to 1</li> <li>Strong attraction for transition metals, even in highly concentrated salt solutions</li> <li>Inactivates enzymes, including DNases, that require divalent cations for activity</li> <li>Larger volumes and special packaging are available upon request</li> </ul> <h2 class="descHeader">Product Options</h2> <table class="pd_table pd_gridlines" border="0"> <tbody> <tr> <td width="450" align="center" valign="top"><strong>Resin</strong></td> <td width="250" align="center" valign="top"><strong>Strength</strong></td> <td width="250" align="center" valign="top"><strong>Grade*</strong></td> <td width="150" align="center" valign="top"><strong>Crosslink</strong></td> <td width="325" align="center" valign="top"><strong>Ionic Form</strong></td> <td width="325" align="center" valign="top"><strong>Dry Mesh</strong></td> <td width="450" align="center" valign="top"><strong>Wet Bead Size &micro;m</strong></td> <td width="319" align="center" valign="top"><strong>MW Limit </strong></td> <td width="319" align="center" valign="top"><strong>Catalog Number</strong></td> </tr> <tr class="pd_colorbackground"> <td align="center" valign="top">Chelex 100</td> <td align="center" valign="top">Weak</td> <td align="center" valign="top">Analytical</td> <td align="center" valign="top">1%</td> <td align="center" valign="top">Sodium</td> <td align="center" valign="top">50&ndash;100</td> <td align="center" valign="top">300&ndash;1,180</td> <td align="center" valign="top">~3,500</td> <td align="center" valign="top"><a href="/en-us/sku/1422822-chelex-100-chelating-resin-analytical-grade-50-ndash-100-mesh-sodium-form-500-g?ID=1422822">1422822</a></td> </tr> <tr> <td align="center" valign="top">Chelex 100</td> <td align="center" valign="top">Weak</td> <td align="center" valign="top">Analytical</td> <td align="center" valign="top">1%</td> <td align="center" valign="top">Sodium</td> <td align="center" valign="top">100&ndash;200</td> <td align="center" valign="top">150&ndash;300</td> <td align="center" valign="top">~3,500</td> <td align="center" valign="top">1422832</td> </tr> <tr class="pd_colorbackground"> <td align="center" valign="top">Chelex 100</td> <td align="center" valign="top">Weak</td> <td align="center" valign="top">Biotech</td> <td align="center" valign="top">1%</td> <td align="center" valign="top">Sodium</td> <td align="center" valign="top">100&ndash;200</td> <td align="center" valign="top">150&ndash;300</td> <td align="center" valign="top">~3,500</td> <td align="center" valign="top"><a href="/en-us/sku/1432832-chelex-100-chelating-resin-biotechnology-grade-100-ndash-200-mesh-sodium-form-100-g?ID=1432832">1432832</a></td> </tr> <tr> <td align="center" valign="top">Chelex 100</td> <td align="center" valign="top">Weak</td> <td align="center" valign="top">Analytical</td> <td align="center" valign="top">1%</td> <td align="center" valign="top">Iron</td> <td align="center" valign="top">100&ndash;200</td> <td align="center" valign="top">150&ndash;300</td> <td align="center" valign="top">~3,500</td> <td align="center" valign="top"><a href="/en-us/sku/1422825-chelex-100-chelating-resin-analytical-grade-100-ndash-200-mesh-iron-form-100g?ID=1422825">1422825</a></td> </tr> <tr class="pd_colorbackground"> <td align="center" valign="top">Chelex 100</td> <td align="center" valign="top">Weak</td> <td align="center" valign="top">Analytical</td> <td align="center" valign="top">1%</td> <td align="center" valign="top">Sodium</td> <td align="center" valign="top">200&ndash;400</td> <td align="center" valign="top">75&ndash;150</td> <td align="center" valign="top">~3,500</td> <td align="center" valign="top"><a href="/en-us/sku/1422842-chelex-100-chelating-resin-analytical-grade-200-ndash-400-mesh-sodium-form-500-g?ID=1422842">1422842</a></td> </tr> <tr> <td align="center" valign="top">Chelex 100</td> <td align="center" valign="top">Weak</td> <td align="center" valign="top">Mol Bio</td> <td align="center" valign="top">1%</td> <td align="center" valign="top">Sodium</td> <td align="center" valign="top">200&ndash;400</td> <td align="center" valign="top">75&ndash;150</td> <td align="center" valign="top">~3,500</td> <td align="center" valign="top"><a href="/en-us/sku/1421253-chelex-100-chelating-resin-molecular-biology-grade-200-ndash-400-mesh-sodium-form-50-g?ID=1421253">1421253</a></td> </tr> </tbody> </table> <p class="caption">*Biotechnology grade resin is certified to contain less than 100 microorganisms per gram; molecular-biology-grade resin is certified to be endo- and exonuclease-free and contain no ligase inhibitors.</p> <h2 class="descHeader">Related Products</h2> <ul> <li><a href="/en-us/product/poly-prep-chromatography-columns">Poly-Prep<sup>&reg;</sup> Chromatography Columns</a> hold up to 2 ml resin, polypropylene</li> <li><a href="/en-us/product/econo-pac-chromatography-columns">Econo-Pac<sup>&reg;</sup> Chromatography Columns</a> hold from 1 to 20 ml resin, polypropylene</li> <li><a href="/en-us/product/glass-econo-column-columns?ID=2cfb1c6e-32e8-4c72-b532-dd39013d707d">Glass Econo-Column<sup>&reg;</sup> Columns </a></li> <li><a href="/en-us/product/instagene-matrix">InstaGene&trade; Matrix</a> for nucleic acid extraction</li> </ul> <h2 class="descHeader">Related Categories</h2> <ul> <li>Selection of <a href="/en-us/category/cation-exchange-resin">Cation Exchange Resins</a> with different specificities, capacities, operating pressures and flow rates</li> <li><a href="/en-us/category/chromatography-systems-components-accessories">Chromatography Systems, Components, and Accessories</a></li> <li><a href="/en-us/category/biologic-low-pressure-liquid-chromatography-systems">BioLogic&trade; Low-Pressure Liquid Chromatography Systems </a></li> <li><a href="/en-us/category/chromatography-resins?ID=f6b4c81f-147b-4130-83ae-d24dbba71eb2">Chromatography Resins </a></li> <li><a href="/en-us/category/chromatography-columns?ID=826b06e0-11cf-4f20-a477-e59ebc32eec1">Chromatography Columns </a></li> </ul> <h2 class="descHeader">Supporting Documents</h2> <ul> <li>Chelex 100 and Chelex 20 Chelating Ion Exchange Resin <a href="/webroot/web/pdf/lsr/literature/LIT200.pdf" target="_blank">Instruction Manual</a></li> <li>Chelex 100 Iron-Form Resin for Glyphosate Cleanup <a href="/webroot/web/pdf/lsr/literature/LIT237.pdf" target="_blank">Instruction Sheet</a></li> <li>InstaGene Matrix <a href="/webroot/web/pdf/lsr/literature/Bulletin_2074.pdf" target="_blank">Product Information Sheet</a></li> <li>Lab-Scale Chromatography Sample Preparation <a href="/webroot/web/pdf/lsr/literature/Bulletin_5777A.pdf" target="_blank">Guide</a></li> </ul> <h2 class="descHeader">Online Resources</h2> <ul> <li><a href="/en-us/applications-technologies/introduction-chromatography">Introduction to Chromatography</a></li> <li><a href="/en-us/applications-technologies/pcr-polymerase-chain-reaction">PCR (Polymerase Chain Reaction)</a></li> <li><a href="/en-us/applications-technologies/introduction-digital-pcr">Introduction to Digital PCR</a></li> </ul> <ul> </ul> /webroot/web/html/lsr/products/SKUs/autopopulated Chelex-100-Chelating-Resin-analytical-grade-100-200-mesh-sodium-form-100-g-1422832.html /webroot/web/html/lsr/products/SKUs/autopopulated/Chelex-100-Chelating-Resin-analytical-grade-100-200-mesh-sodium-form-100-g-1422832.html false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1422832&CartQuote=QT&quantity= false true false $856.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1422832&AddToDefaultWishList=true&quantity= true false true true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1422832&quantity= 1422832
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Chelex® 100 Chelating Resin, analytical grade, 100–200 mesh, iron form, 100 g

1422825
100 g, cation exchange resin, iron form, 1% crosslinkage, 100–200 dry mesh size, 150–300 µm wet bead size, ~3,500 MW limit

3 1422825 /templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Media/142-2825_0921120700681.xml 1422825 09/21/09 12:07 PM N en 09/21/19 12:07 PM true true internet/product_SKU AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA LSR 2018-02-01 00:17:44 1422825-chelex-100-chelating-resin-analytical-grade-100-ndash-200-mesh-iron-form-100g 1422825 Chelex 100 Resin Chelex<sup>®</sup> 100 Chelating Resin, analytical grade, 100&ndash;200 mesh, iron form, 100&nbsp;g 100 g, cation exchange resin, iron form, 1% crosslinkage, 100–200 dry mesh size, 150–300 µm wet bead size, ~3,500 MW limit 100 g, cation exchange resin, iron form, 1% crosslinkage, 100–200 dry mesh size, 150–300 µm wet bead size, ~3,500 MW limit 100 g, cation exchange resin, iron form, 1% crosslinkage, 100–200 dry mesh size, 150–300 µm wet bead size, ~3,500 MW limit /webroot/web/images/lsr/products/chromatography/sku_thumb/global/142-2825_tn.jpg /webroot/web/images/lsr/products/chromatography/sku_view/global/142-2825_view.jpg /webroot/web/images/lsr/products/chromatography/sku_feature/global/142-2825_feature.jpg Chelex® 100 Chelating Resin, analytical grade, 100-200 mesh, iron form, 100 g preview RepEx 142-2825 100 g, cation exchange resin, iron form, 1% crosslinkage, 100–200 dry mesh size, 150–300 µm wet bead size, ~3,500 MW limit 142-2825, 1422825, chelex, 100, resin, ion, cation, exchange, iex, chromatography, purification, fplc, media 09/21/09 12:07 PM 09/21/19 12:07 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Chromatography/Chromatography-Media N 0 <p>Choose analytical grade Chelex 100 Resin, a weak cation exchange media, for chelation, catalysis, and other cation exchange protocols. Use this iron-form resin for gravity or low-pressure chromatography.</p> <ul> <li>Medium-mesh resin is effective for both preparative and analytical protocols</li> <li>Can be used as a catalyst for phosphodiester hydrolysis</li> <li>Use for glyphosate cleanup</li> <li>Larger volumes and special packaging are available upon request</li> </ul> <h2 class="descHeader">Product Options</h2> <table class="pd_table pd_gridlines" border="0"> <tbody> <tr> <td width="450" align="center" valign="top"><strong>Resin</strong></td> <td width="250" align="center" valign="top"><strong>Strength</strong></td> <td width="250" align="center" valign="top"><strong>Grade*</strong></td> <td width="150" align="center" valign="top"><strong>Crosslink</strong></td> <td width="325" align="center" valign="top"><strong>Ionic Form</strong></td> <td width="325" align="center" valign="top"><strong>Dry Mesh</strong></td> <td width="450" align="center" valign="top"><strong>Wet Bead Size &micro;m</strong></td> <td width="319" align="center" valign="top"><strong>MW Limit </strong></td> <td width="319" align="center" valign="top"><strong>Catalog Number</strong></td> </tr> <tr class="pd_colorbackground"> <td align="center" valign="top">Chelex 100</td> <td align="center" valign="top">Weak</td> <td align="center" valign="top">Analytical</td> <td align="center" valign="top">1%</td> <td align="center" valign="top">Sodium</td> <td align="center" valign="top">50&ndash;100</td> <td align="center" valign="top">300&ndash;1,180</td> <td align="center" valign="top">~3,500</td> <td align="center" valign="top"><a href="/en-us/sku/1422822-chelex-100-chelating-resin-analytical-grade-50-ndash-100-mesh-sodium-form-500-g?ID=1422822">1422822</a></td> </tr> <tr> <td align="center" valign="top">Chelex 100</td> <td align="center" valign="top">Weak</td> <td align="center" valign="top">Analytical</td> <td align="center" valign="top">1%</td> <td align="center" valign="top">Sodium</td> <td align="center" valign="top">100&ndash;200</td> <td align="center" valign="top">150&ndash;300</td> <td align="center" valign="top">~3,500</td> <td align="center" valign="top"><a href="/en-us/sku/1422832-chelex-100-chelating-resin-analytical-grade-100-ndash-200-mesh-sodium-form-500-g?ID=1422832">1422832</a></td> </tr> <tr class="pd_colorbackground"> <td align="center" valign="top">Chelex 100</td> <td align="center" valign="top">Weak</td> <td align="center" valign="top">Biotech</td> <td align="center" valign="top">1%</td> <td align="center" valign="top">Sodium</td> <td align="center" valign="top">100&ndash;200</td> <td align="center" valign="top">150&ndash;300</td> <td align="center" valign="top">~3,500</td> <td align="center" valign="top"><a href="/en-us/sku/1432832-chelex-100-chelating-resin-biotechnology-grade-100-ndash-200-mesh-sodium-form-100-g?ID=1432832">1432832</a></td> </tr> <tr> <td align="center" valign="top">Chelex 100</td> <td align="center" valign="top">Weak</td> <td align="center" valign="top">Analytical</td> <td align="center" valign="top">1%</td> <td align="center" valign="top">Iron</td> <td align="center" valign="top">100&ndash;200</td> <td align="center" valign="top">150&ndash;300</td> <td align="center" valign="top">~3,500</td> <td align="center" valign="top">1422825</td> </tr> <tr class="pd_colorbackground"> <td align="center" valign="top">Chelex 100</td> <td align="center" valign="top">Weak</td> <td align="center" valign="top">Analytical</td> <td align="center" valign="top">1%</td> <td align="center" valign="top">Sodium</td> <td align="center" valign="top">200&ndash;400</td> <td align="center" valign="top">75&ndash;150</td> <td align="center" valign="top">~3,500</td> <td align="center" valign="top"><a href="/en-us/sku/1422842-chelex-100-chelating-resin-analytical-grade-200-ndash-400-mesh-sodium-form-500-g?ID=1422842">1422842</a></td> </tr> <tr> <td align="center" valign="top">Chelex 100</td> <td align="center" valign="top">Weak</td> <td align="center" valign="top">Mol Bio</td> <td align="center" valign="top">1%</td> <td align="center" valign="top">Sodium</td> <td align="center" valign="top">200&ndash;400</td> <td align="center" valign="top">75&ndash;150</td> <td align="center" valign="top">~3,500</td> <td align="center" valign="top"><a href="/en-us/sku/1421253-chelex-100-chelating-resin-molecular-biology-grade-200-ndash-400-mesh-sodium-form-50-g?ID=1421253">1421253</a></td> </tr> </tbody> </table> <p class="caption">*Biotechnology grade resin is certified to contain less than 100 microorganisms per gram; molecular-biology-grade resin is certified to be endo- and exonuclease-free and contain no ligase inhibitors.</p> <h2 class="descHeader">Related Products</h2> <ul> <li><a href="/en-us/product/poly-prep-chromatography-columns">Poly-Prep<sup>&reg;</sup> Chromatography Columns</a> hold up to 2 ml resin, polypropylene</li> <li><a href="/en-us/product/econo-pac-chromatography-columns">Econo-Pac<sup>&reg;</sup> Chromatography Columns</a> hold from 1 to 20 ml resin, polypropylene</li> <li><a href="/en-us/product/glass-econo-column-columns?ID=2cfb1c6e-32e8-4c72-b532-dd39013d707d">Glass Econo-Column<sup>&reg;</sup> Columns </a></li> </ul> <h2 class="descHeader">Related Categories</h2> <ul> <li>Selection of <a href="/en-us/category/cation-exchange-resin">Cation Exchange Resins</a> with different specificities, capacities, operating pressures and flow rates</li> <li><a href="/en-us/category/chromatography-systems-components-accessories">Chromatography Systems, Components, and Accessories</a></li> <li><a href="/en-us/category/biologic-low-pressure-liquid-chromatography-systems">BioLogic&trade; Low-Pressure Liquid Chromatography Systems </a></li> <li><a href="/en-us/category/chromatography-resins?ID=f6b4c81f-147b-4130-83ae-d24dbba71eb2">Chromatography Resins </a></li> <li><a href="/en-us/category/chromatography-columns?ID=826b06e0-11cf-4f20-a477-e59ebc32eec1">Chromatography Columns </a></li> </ul> <h2 class="descHeader">Supporting Documents</h2> <ul> <li>Chelex 100 and Chelex 20 Chelating Ion Exchange Resin <a href="/webroot/web/pdf/lsr/literature/LIT200.pdf" target="_blank">Instruction Manual</a></li> <li>Chelex 100 Iron-Form Resin for Glyphosate Cleanup <a href="/webroot/web/pdf/lsr/literature/LIT237.pdf" target="_blank">Instruction Sheet</a></li> <li>Lab-Scale Chromatography Sample Preparation <a href="/webroot/web/pdf/lsr/literature/Bulletin_5777A.pdf" target="_blank">Guide</a></li> </ul> <h2 class="descHeader">Online Resources</h2> <ul> <li><a href="/en-us/applications-technologies/introduction-chromatography">Introduction to Chromatography</a></li> </ul> <ul> </ul> /webroot/web/html/lsr/products/SKUs/autopopulated Chelex-100-Chelating-Resin-analytical-grade-100-200-mesh-iron-form-100-g-1422825.html /webroot/web/html/lsr/products/SKUs/autopopulated/Chelex-100-Chelating-Resin-analytical-grade-100-200-mesh-iron-form-100-g-1422825.html false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1422825&CartQuote=QT&quantity= false true false $856.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1422825&AddToDefaultWishList=true&quantity= true false true true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1422825&quantity= 1422825
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Chelex® 100 Chelating Resin, molecular biology grade, 200–400 mesh, sodium form, 50 g

1421253
50 g, molecular biology grade cation exchange resin, sodium form, 1% crosslinkage, 200–400 dry mesh size, 75–150 µm wet bead size, ~3,500 MW limit

4 1421253 /templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Media/142-1253_0921120700739.xml 1421253 09/21/09 12:07 PM N en 09/21/19 12:07 PM true true internet/product_SKU AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA LSR 2018-02-01 00:17:44 1421253-chelex-100-chelating-resin-molecular-biology-grade-200-ndash-400-mesh-sodium-form-50-g 1421253 Chelex 100 Resin Chelex<sup>®</sup> 100 Chelating Resin, molecular biology grade, 200&ndash;400 mesh, sodium form, 50 g 50 g, molecular biology grade cation exchange resin, sodium form, 1% crosslinkage, 200–400 dry mesh size, 75–150 µm wet bead size, ~3,500 MW limit 50 g, molecular biology grade cation exchange resin, sodium form, 1% crosslinkage, 200–400 dry mesh size, 75–150 µm wet bead size, ~3,500 MW limit 50 g, molecular biology grade cation exchange resin, sodium form, 1% crosslinkage, 200–400 dry mesh size, 75–150 µm wet bead size, ~3,500 MW limit /webroot/web/images/lsr/products/chromatography/sku_thumb/global/142-1253_tn.jpg /webroot/web/images/lsr/products/chromatography/sku_view/global/142-1253_view.jpg /webroot/web/images/lsr/products/chromatography/sku_feature/global/142-1253_feature.jpg preview RepEx 142-1253 50 g, molecular biology grade cation exchange resin, sodium form, 1% crosslinkage, 200–400 dry mesh size, 75–150 µm wet bead size, ~3,500 MW limit 142-1253, 1421253, chelex, 100, resin, ion, cation, exchange, iex, chromatography, purification, fplc, media 09/21/09 12:07 PM 09/21/19 12:07 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Chromatography/Chromatography-Media N 0 <p>Choose molecular biology grade Chelex 100 Resin, a weak cation exchange media, for chelation, DNA extraction for PCR, and other cation exchange protocols. Use this sodium-form resin for gravity or low-pressure chromatography.</p> <ul> <li>Fine-mesh resin is effective for high resolution analytical protocols</li> <li>Selectivity for divalent over monovalent ions is approximately 5,000 to 1</li> <li>Strong attraction for transition metals, even in highly concentrated salt solutions</li> <li>Inactivates enzymes, including DNases, that require divalent cations for activity</li> <li>Certified to be endo- and exonuclease-free, and to contain no ligase inhibitors</li> <li>Larger volumes and special packaging are available upon request</li> </ul> <h2 class="descHeader">Product Options</h2> <table class="pd_table pd_gridlines" border="0"> <tbody> <tr> <td width="450" align="center" valign="top"><strong>Resin</strong></td> <td width="250" align="center" valign="top"><strong>Strength</strong></td> <td width="250" align="center" valign="top"><strong>Grade*</strong></td> <td width="150" align="center" valign="top"><strong>Crosslink</strong></td> <td width="325" align="center" valign="top"><strong>Ionic Form</strong></td> <td width="325" align="center" valign="top"><strong>Dry Mesh</strong></td> <td width="450" align="center" valign="top"><strong>Wet Bead Size &micro;m</strong></td> <td width="319" align="center" valign="top"><strong>MW Limit </strong></td> <td width="319" align="center" valign="top"><strong>Catalog Number</strong></td> </tr> <tr class="pd_colorbackground"> <td align="center" valign="top">Chelex 100</td> <td align="center" valign="top">Weak</td> <td align="center" valign="top">Analytical</td> <td align="center" valign="top">1%</td> <td align="center" valign="top">Sodium</td> <td align="center" valign="top">50&ndash;100</td> <td align="center" valign="top">300&ndash;1,180</td> <td align="center" valign="top">~3,500</td> <td align="center" valign="top"><a href="/en-us/sku/1422822-chelex-100-chelating-resin-analytical-grade-50-ndash-100-mesh-sodium-form-500-g?ID=1422822">1422822</a></td> </tr> <tr> <td align="center" valign="top">Chelex 100</td> <td align="center" valign="top">Weak</td> <td align="center" valign="top">Analytical</td> <td align="center" valign="top">1%</td> <td align="center" valign="top">Sodium</td> <td align="center" valign="top">100&ndash;200</td> <td align="center" valign="top">150&ndash;300</td> <td align="center" valign="top">~3,500</td> <td align="center" valign="top"><a href="/en-us/sku/1422832-chelex-100-chelating-resin-analytical-grade-100-ndash-200-mesh-sodium-form-500-g?ID=1422832">1422832</a></td> </tr> <tr class="pd_colorbackground"> <td align="center" valign="top">Chelex 100</td> <td align="center" valign="top">Weak</td> <td align="center" valign="top">Biotech</td> <td align="center" valign="top">1%</td> <td align="center" valign="top">Sodium</td> <td align="center" valign="top">100&ndash;200</td> <td align="center" valign="top">150&ndash;300</td> <td align="center" valign="top">~3,500</td> <td align="center" valign="top"><a href="/en-us/sku/1432832-chelex-100-chelating-resin-biotechnology-grade-100-ndash-200-mesh-sodium-form-100-g?ID=1432832">1432832</a></td> </tr> <tr> <td align="center" valign="top">Chelex 100</td> <td align="center" valign="top">Weak</td> <td align="center" valign="top">Analytical</td> <td align="center" valign="top">1%</td> <td align="center" valign="top">Iron</td> <td align="center" valign="top">100&ndash;200</td> <td align="center" valign="top">150&ndash;300</td> <td align="center" valign="top">~3,500</td> <td align="center" valign="top"><a href="/en-us/sku/1422825-chelex-100-chelating-resin-analytical-grade-100-ndash-200-mesh-iron-form-100g?ID=1422825">1422825</a></td> </tr> <tr class="pd_colorbackground"> <td align="center" valign="top">Chelex 100</td> <td align="center" valign="top">Weak</td> <td align="center" valign="top">Analytical</td> <td align="center" valign="top">1%</td> <td align="center" valign="top">Sodium</td> <td align="center" valign="top">200&ndash;400</td> <td align="center" valign="top">75&ndash;150</td> <td align="center" valign="top">~3,500</td> <td align="center" valign="top"><a href="/en-us/sku/1422842-chelex-100-chelating-resin-analytical-grade-200-ndash-400-mesh-sodium-form-500-g?ID=1422842">1422842</a></td> </tr> <tr> <td align="center" valign="top">Chelex 100</td> <td align="center" valign="top">Weak</td> <td align="center" valign="top">Mol Bio</td> <td align="center" valign="top">1%</td> <td align="center" valign="top">Sodium</td> <td align="center" valign="top">200&ndash;400</td> <td align="center" valign="top">75&ndash;150</td> <td align="center" valign="top">~3,500</td> <td align="center" valign="top">1421253</td> </tr> </tbody> </table> <p class="caption">*Biotechnology grade resin is certified to contain less than 100 microorganisms per gram; molecular-biology-grade resin is certified to be endo- and exonuclease-free and contain no ligase inhibitors.</p> <h2 class="descHeader">Related Products</h2> <ul> <li><a href="/en-us/product/poly-prep-chromatography-columns">Poly-Prep<sup>&reg;</sup> Chromatography Columns</a> hold up to 2 ml resin, polypropylene</li> <li><a href="/en-us/product/econo-pac-chromatography-columns">Econo-Pac<sup>&reg;</sup> Chromatography Columns</a> hold from 1 to 20 ml resin, polypropylene</li> <li><a href="/en-us/product/glass-econo-column-columns?ID=2cfb1c6e-32e8-4c72-b532-dd39013d707d">Glass Econo-Column<sup>&reg;</sup> Columns </a></li> <li><a href="/en-us/product/instagene-matrix">InstaGene&trade; Matrix</a> for nucleic acid extraction</li> </ul> <h2 class="descHeader">Related Categories</h2> <ul> <li>Selection of <a href="/en-us/category/cation-exchange-resin">Cation Exchange Resins</a> with different specificities, capacities, operating pressures and flow rates</li> <li><a href="/en-us/category/chromatography-systems-components-accessories">Chromatography Systems, Components, and Accessories</a></li> <li><a href="/en-us/category/biologic-low-pressure-liquid-chromatography-systems">BioLogic&trade; Low-Pressure Liquid Chromatography Systems </a></li> <li><a href="/en-us/category/chromatography-resins?ID=f6b4c81f-147b-4130-83ae-d24dbba71eb2">Chromatography Resins </a></li> <li><a href="/en-us/category/chromatography-columns?ID=826b06e0-11cf-4f20-a477-e59ebc32eec1">Chromatography Columns </a></li> </ul> <h2 class="descHeader">Supporting Documents</h2> <ul> <li>Chelex 100 and Chelex 20 Chelating Ion Exchange Resin <a href="/webroot/web/pdf/lsr/literature/LIT200.pdf" target="_blank">Instruction Manual</a></li> <li>Chelex 100 Iron-Form Resin for Glyphosate Cleanup <a href="/webroot/web/pdf/lsr/literature/LIT237.pdf" target="_blank">Instruction Sheet</a></li> <li>InstaGene Matrix <a href="/webroot/web/pdf/lsr/literature/Bulletin_2074.pdf" target="_blank">Product Information Sheet</a></li> <li>Lab-Scale Chromatography Sample Preparation <a href="/webroot/web/pdf/lsr/literature/Bulletin_5777A.pdf" target="_blank">Guide</a></li> </ul> <h2 class="descHeader">Online Resources</h2> <ul> <li><a href="/en-us/applications-technologies/introduction-chromatography">Introduction to Chromatography</a></li> <li><a href="/en-us/applications-technologies/pcr-polymerase-chain-reaction">PCR (Polymerase Chain Reaction)</a></li> <li><a href="/en-us/applications-technologies/introduction-digital-pcr">Introduction to Digital PCR</a></li> </ul> <ul> </ul> /webroot/web/html/lsr/products/SKUs/autopopulated Chelex-100-Chelating-Resin-molecular-biology-grade-200-400-mesh-sodium-form-50-g-1421253.html /webroot/web/html/lsr/products/SKUs/autopopulated/Chelex-100-Chelating-Resin-molecular-biology-grade-200-400-mesh-sodium-form-50-g-1421253.html false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1421253&CartQuote=QT&quantity= false true false $210.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1421253&AddToDefaultWishList=true&quantity= true false true true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1421253&quantity= 1421253
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Chelex® 100 Chelating Resin, biotechnology grade, 100–200 mesh, sodium form, 100 g

1432832
100 g, biotechnology grade cation exchange resin, sodium form, 1% crosslinkage, 100–200 dry mesh size, 150–300 µm wet bead size, ~3,500 MW limit

5 1432832 /templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Media/143-2832_0921120700703.xml 1432832 09/21/09 12:07 PM N en 09/21/19 12:07 PM true true internet/product_SKU AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA LSR 2018-02-01 00:17:45 1432832-chelex-100-chelating-resin-biotechnology-grade-100-ndash-200-mesh-sodium-form-100-g 1432832 Chelex 100 Resin Chelex<sup>®</sup> 100 Chelating Resin, biotechnology grade, 100&ndash;200 mesh, sodium form, 100 g 100 g, biotechnology grade cation exchange resin, sodium form, 1% crosslinkage, 100–200 dry mesh size, 150–300 µm wet bead size, ~3,500 MW limit 100 g, biotechnology grade cation exchange resin, sodium form, 1% crosslinkage, 100–200 dry mesh size, 150–300 µm wet bead size, ~3,500 MW limit 100 g, biotechnology grade cation exchange resin, sodium form, 1% crosslinkage, 100–200 dry mesh size, 150–300 µm wet bead size, ~3,500 MW limit /webroot/web/images/ps/products/ion_exchange_media/sku_thumb/global/143-2832_tn.jpg /webroot/web/images/ps/products/ion_exchange_media/sku_view/global/143-2832_view.jpg /webroot/web/images/ps/products/ion_exchange_media/sku_feature/global/143-2832_feature.jpg Chelex<sup>®</sup> 100 Chelating Resin, biotechnology grade, 100-200 mesh, sodium form, 500 g preview RepEx 143-2832 100 g, biotechnology grade cation exchange resin, sodium form, 1% crosslinkage, 100–200 dry mesh size, 150–300 µm wet bead size, ~3,500 MW limit 143-2832, 1432832, chelex, 100, resin, ion, cation, exchange, iex, chromatography, purification, fplc, media 09/21/09 12:07 PM 09/21/19 12:07 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Chromatography/Chromatography-Media N 0 <p>Choose biotechnology grade Chelex 100 Resin, a weak cation exchange media, for chelation, DNA extraction for PCR, and other cation exchange protocols. Use this sodium-form resin for gravity or low-pressure chromatography.</p> <ul> <li>Medium-mesh resin is effective for both preparative and analytical protocols</li> <li>Selectivity for divalent over monovalent ions is approximately 5,000 to 1</li> <li>Strong attraction for transition metals, even in highly concentrated salt solutions</li> <li>Inactivates enzymes, including DNases, that require divalent cations for activity</li> <li>Certified to contain less than 100 microorganisms per gram</li> <li>Larger volumes and special packaging are available upon request</li> </ul> <h2 class="descHeader">Product Options</h2> <table class="pd_table pd_gridlines" border="0"> <tbody> <tr> <td width="450" align="center" valign="top"><strong>Resin</strong></td> <td width="250" align="center" valign="top"><strong>Strength</strong></td> <td width="250" align="center" valign="top"><strong>Grade*</strong></td> <td width="150" align="center" valign="top"><strong>Crosslink</strong></td> <td width="325" align="center" valign="top"><strong>Ionic Form</strong></td> <td width="325" align="center" valign="top"><strong>Dry Mesh</strong></td> <td width="450" align="center" valign="top"><strong>Wet Bead Size &micro;m</strong></td> <td width="319" align="center" valign="top"><strong>MW Limit </strong></td> <td width="319" align="center" valign="top"><strong>Catalog Number</strong></td> </tr> <tr class="pd_colorbackground"> <td align="center" valign="top">Chelex 100</td> <td align="center" valign="top">Weak</td> <td align="center" valign="top">Analytical</td> <td align="center" valign="top">1%</td> <td align="center" valign="top">Sodium</td> <td align="center" valign="top">50&ndash;100</td> <td align="center" valign="top">300&ndash;1,180</td> <td align="center" valign="top">~3,500</td> <td align="center" valign="top"><a href="/en-us/sku/1422822-chelex-100-chelating-resin-analytical-grade-50-ndash-100-mesh-sodium-form-500-g?ID=1422822">1422822</a></td> </tr> <tr> <td align="center" valign="top">Chelex 100</td> <td align="center" valign="top">Weak</td> <td align="center" valign="top">Analytical</td> <td align="center" valign="top">1%</td> <td align="center" valign="top">Sodium</td> <td align="center" valign="top">100&ndash;200</td> <td align="center" valign="top">150&ndash;300</td> <td align="center" valign="top">~3,500</td> <td align="center" valign="top"><a href="/en-us/sku/1422832-chelex-100-chelating-resin-analytical-grade-100-ndash-200-mesh-sodium-form-500-g?ID=1422832">1422832</a></td> </tr> <tr class="pd_colorbackground"> <td align="center" valign="top">Chelex 100</td> <td align="center" valign="top">Weak</td> <td align="center" valign="top">Biotech</td> <td align="center" valign="top">1%</td> <td align="center" valign="top">Sodium</td> <td align="center" valign="top">100&ndash;200</td> <td align="center" valign="top">150&ndash;300</td> <td align="center" valign="top">~3,500</td> <td align="center" valign="top">1432832</td> </tr> <tr> <td align="center" valign="top">Chelex 100</td> <td align="center" valign="top">Weak</td> <td align="center" valign="top">Analytical</td> <td align="center" valign="top">1%</td> <td align="center" valign="top">Iron</td> <td align="center" valign="top">100&ndash;200</td> <td align="center" valign="top">150&ndash;300</td> <td align="center" valign="top">~3,500</td> <td align="center" valign="top"><a href="/en-us/sku/1422825-chelex-100-chelating-resin-analytical-grade-100-ndash-200-mesh-iron-form-100g?ID=1422825">1422825</a></td> </tr> <tr class="pd_colorbackground"> <td align="center" valign="top">Chelex 100</td> <td align="center" valign="top">Weak</td> <td align="center" valign="top">Analytical</td> <td align="center" valign="top">1%</td> <td align="center" valign="top">Sodium</td> <td align="center" valign="top">200&ndash;400</td> <td align="center" valign="top">75&ndash;150</td> <td align="center" valign="top">~3,500</td> <td align="center" valign="top"><a href="/en-us/sku/1422842-chelex-100-chelating-resin-analytical-grade-200-ndash-400-mesh-sodium-form-500-g?ID=1422842">1422842</a></td> </tr> <tr> <td align="center" valign="top">Chelex 100</td> <td align="center" valign="top">Weak</td> <td align="center" valign="top">Mol Bio</td> <td align="center" valign="top">1%</td> <td align="center" valign="top">Sodium</td> <td align="center" valign="top">200&ndash;400</td> <td align="center" valign="top">75&ndash;150</td> <td align="center" valign="top">~3,500</td> <td align="center" valign="top"><a href="/en-us/sku/1421253-chelex-100-chelating-resin-molecular-biology-grade-200-ndash-400-mesh-sodium-form-50-g?ID=1421253">1421253</a></td> </tr> </tbody> </table> <p class="caption">*Biotechnology grade resin is certified to contain less than 100 microorganisms per gram; molecular-biology-grade resin is certified to be endo- and exonuclease-free and contain no ligase inhibitors.</p> <h2 class="descHeader">Related Products</h2> <ul> <li><a href="/en-us/product/poly-prep-chromatography-columns">Poly-Prep<sup>&reg;</sup> Chromatography Columns</a> hold up to 2 ml resin, polypropylene</li> <li><a href="/en-us/product/econo-pac-chromatography-columns">Econo-Pac<sup>&reg;</sup> Chromatography Columns</a> hold from 1 to 20 ml resin, polypropylene</li> <li><a href="/en-us/product/glass-econo-column-columns?ID=2cfb1c6e-32e8-4c72-b532-dd39013d707d">Glass Econo-Column<sup>&reg;</sup> Columns </a></li> <li><a href="/en-us/product/instagene-matrix">InstaGene&trade; Matrix</a> for nucleic acid extraction</li> </ul> <h2 class="descHeader">Related Categories</h2> <ul> <li>Selection of <a href="/en-us/category/cation-exchange-resin">Cation Exchange Resins</a> with different specificities, capacities, operating pressures and flow rates</li> <li><a href="/en-us/category/chromatography-systems-components-accessories">Chromatography Systems, Components, and Accessories</a></li> <li><a href="/en-us/category/biologic-low-pressure-liquid-chromatography-systems">BioLogic&trade; Low-Pressure Liquid Chromatography Systems </a></li> <li><a href="/en-us/category/chromatography-resins?ID=f6b4c81f-147b-4130-83ae-d24dbba71eb2">Chromatography Resins </a></li> <li><a href="/en-us/category/chromatography-columns?ID=826b06e0-11cf-4f20-a477-e59ebc32eec1">Chromatography Columns </a></li> </ul> <h2 class="descHeader">Supporting Documents</h2> <ul> <li>Chelex 100 and Chelex 20 Chelating Ion Exchange Resin <a href="/webroot/web/pdf/lsr/literature/LIT200.pdf" target="_blank">Instruction Manual</a></li> <li>Chelex 100 Iron-Form Resin for Glyphosate Cleanup <a href="/webroot/web/pdf/lsr/literature/LIT237.pdf" target="_blank">Instruction Sheet</a></li> <li>InstaGene Matrix <a href="/webroot/web/pdf/lsr/literature/Bulletin_2074.pdf" target="_blank">Product Information Sheet</a></li> <li>Lab-Scale Chromatography Sample Preparation <a href="/webroot/web/pdf/lsr/literature/Bulletin_5777A.pdf" target="_blank">Guide</a></li> </ul> <h2 class="descHeader">Online Resources</h2> <ul> <li><a href="/en-us/applications-technologies/introduction-chromatography">Introduction to Chromatography</a></li> <li><a href="/en-us/applications-technologies/pcr-polymerase-chain-reaction">PCR (Polymerase Chain Reaction)</a></li> <li><a href="/en-us/applications-technologies/introduction-digital-pcr">Introduction to Digital PCR</a></li> </ul> <ul> </ul> /webroot/web/html/lsr/products/SKUs/autopopulated Chelex-100-Chelating-Resin,-biotechnology-grade-100-200-mesh-sodium-form-500-g-1432832.html /webroot/web/html/lsr/products/SKUs/autopopulated/Chelex-100-Chelating-Resin,-biotechnology-grade-100-200-mesh-sodium-form-500-g-1432832.html false false false /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1432832&CartQuote=QT&quantity= false true false $452.00 false null /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1432832&AddToDefaultWishList=true&quantity= true false true true false true true true /prd/en/US/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1432832&quantity= 1432832
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Communication error

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Number Description Options
LIT237 Instruction Sheet, Chelex 100 Iron Form Resin for Glyphosate Cleanup, Rev B Click to download
LIT200 Instruction Manual, Chelex 100 and Chelex 20 Chelating Ion Exchange Resin, Rev B Click to download
6908 Speciality Beads: Chelex® 100 Resin Flier, Ver A Click to download

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