3–8% Criterion™ XT Tris-Acetate Protein Gel, 12+2 well, 45 µl #3450129



Pkg of 1, 3–8% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ Electrophoresis Cells
Safety Data Sheet

Safety Data Sheet



Choose this versatile midi Criterion XT Tris-Acetate Gel for denaturing or nondenaturing PAGE. The gels are made without SDS, which allow the sample buffer and running buffer to determine the separation mechanism.

  • For native PAGE, use Native Sample and Tris/Glycine Native Running Buffers
  • For separation by molecular weight, use XT Sample and XT Tricine Running Buffers
  • Neutral pH (pH 7.0) delays acrylamide hydrolysis as compared to traditional Laemmli systems
  • 8-month shelf life
  • Separation range is ~40–400 kD under denaturing conditions
  • Two wells available for markers or other references
  • Can be loaded with a multichannel pipet

Under denaturing conditions, with the SDS-containing Tricine running buffer, the discontinuous acetate and Tricine ion fronts form moving boundaries to stack and then separate proteins by molecular weight. During native electrophoresis, where proteins are separated by mass-to-charge ratio, discontinuous acetate and glycine ion fronts form moving boundaries to stack and separate proteins by both size and charge.

Packaging Options

Criterion XT Tris-Acetate Midi Protein Gels are available as 3–8% (separation range ~40–400 kD) or 7% (separation range ~35–250 kD) gels, with a selection of different well configurations.

Well Configuration, Volume Catalog Number
3–8% 7%
12+2 well, 45 µl 3450129 3450135
18 well, 30 µl 3450130 3450136
26 well, 15 µl 3450131 3450137
11 cm IPG/prep+1 well, 550 µl 3450133

Related Products

  • Native Sample Buffer (1610738)
  • XT Sample Buffer, 4x, denaturing (1610791)
  • Tris/Glycine Native Running Buffer, 10x (1610734)
  • XT Tricine Running Buffer, 20x, denaturing (1610790)
  • XT Reducing Agent, 20x (1610792)
  • XT Tricine Buffer Kit (1610797), XT Tricine Running Buffer, XT Sample buffer, and XT Reducing Agent

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Supporting Documents

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Sample volume, µl
Shelf life, months*
Gel storage conditions
Store flat at 2–8°C; do not freeze
Recommended sample buffer
XT sample buffer + XT reducing agent
Recommended running buffer
XT Tricine running buffer
Gel dimensions (W x L x thickness), cm
13.3 x 8.7 x 0.1
Cassette dimensions (W x L x thickness), cm
15.0 x 10.6 x 0.53
Cassette material
Styrene copolymer
Comb material
Storage tray material
* From date of manufacture.