iQ-Check Listeria spp. PCR Detection Kit

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Overview

Complete sample preparation and PCR kit for the rapid detection of Listeria spp in food and environmental samples. Next-day results.

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Description

Description

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Due to the sensitivity and specificity of PCR, results can be obtained shortly following a single 18–24 hr enrichment in a selective medium.

Highly Specific and Reliable

The iQ-Check Listeria spp. PCR Detection Kit uses an optimized system of primers and probes to ensure high specificity and eliminate cross-reactions. It is designed as a multiplex reaction that includes an internal inhibition control that is amplified in parallel with the target DNA for a reliable result.

Maximum Flexibility

Developed as an open and flexible system, the iQ-Check Listeria spp. Kit can be used for up to 94 samples. Reactions can be run on low- or high-throughput Bio-Rad instruments, depending on the laboratory’s needs. Different iQ-Check assays can be run in parallel on the same plate.

Validated Protocols

The iQ-Check Listeria spp. Kit protocol has been validated by several certification bodies. These validation studies have shown that the kit is equivalent to or better than the various reference methods evaluated and it provides results in much less time.

A Complete Solution

Bio-Rad offers a complete solution with standardized reagents, such as enrichment media, PCR kits, confirmation agars, and PCR detection systems, in addition to software for automated analysis specific to each test.

Kit Components

  • Lysis reagent for DNA extraction
  • Fluorescent probes
  • PCR amplification mix
  • Positive and negative PCR controls

Specifications

Specifications

Ordering

Ordering

items

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3578113
Pkg of 1 kit, includes lysis reagent, fluorescent probes, PCR amplification mix, positive and negative controls, for 94 samples
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3594970
Pkg of 1, 1 vial of lyophilized iQ-Check Free DNA Removal Reagent (to make 10 ml) and 1 vial of iQ-Check 10x Activation Buffer (9 ml)
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3555703
Pkg of 6, 225 ml bottles of liquid media
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3564703
Pkg of 1, 500 g of dehydated broth

Accessories

Accessories

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TLS0851XTU
Pkg of 120, thin-wall polypropylene 8–tube strips for PCR and low volume reactions, white, (960 tubes)
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TCS0803XTU
Pkg of 120, 8-cap strips for 0.2 ml tubes and plates for PCR and optical reactions, ultraclear (960 caps)
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MLL4851XTU
Pkg of 50, white PCR plate, 8 x 6 format, one-component polypropylene, can be cut for fewer samples
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HSP9655XTU
Pkg of 50, white shell/white well PCR plate for higher fluorescent signals, rigid 2-component design
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HSP9955XTU
Pkg of 50, white shell/white well PCR plate for higher fluorescent signals, barcoded, 7-digit sequential, Code 128, rigid 2-component design

Equipment

Equipment

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3600037

Modular thermal cycler platform, includes C1000 Touch Thermal Cycler chassis, CFX96 Deep Well Optical Reaction Module, cables. To be used with CFX Manager Software, Industrial Diagnostic Edition.

Documents

Documents

Number Description Options
10000123536
iQ-Check Listeria spp. Kit User Guide (English, French, German, Italian, Portuguese, Spanish)
FSD_16439
iQ-Check Real-Time PCR Solution Brochure
7376
Performance summary, iQ-Check Listeria spp.
6960
iQ-Check Prep Solution Brochure
7117
iQ-Check Listeria spp kit Quick Guide: Easy II Extraction Deep Well Protocol Using the Thermoshaker
3240
iQ-Check PCR Kits Easy I and II Extraction TUBE Protocol Quick Guide
10000136002
Listeria Special Broth (LSB), User Guide (English, French, German, Italian, Portuguese, Spanish)
TN_Listeria
Internal Validation of iQ-Check® Listeria spp. Kit When Analyzing Five-Sponge Wet-Pooled Environmental Samples
10000058391
iQ-Check Free DNA Removal Solution Product Insert
7322
iQ-Check Free DNA Removal Solution — Quick Guide
6834
iQ-Check Free DNA Removal Solution Brochure
10000119734
iQ-Check Purification Reagent Technical sheet
FSD_17933
Food Safety and Quality Reference Guide
3564703_3564753_GHS
LSB (Listeria Special Broth)
7274
Complete Solutions for Service Laboratories