RAPID'L.mono Medium

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Overview

Selective chromogenic medium for the direct detection and enumeration of Listeria monocytogenes and Listeria spp. in food and environmental samples. 24 hr to results.

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Description

Description

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RAPID'L.mono Medium is a selective chromogenic medium for the direct detection and enumeration of Listeria monocytogenes and Listeria spp. in food and environmental samples.

Key Benefits

  • Fast
    • L. monocytogenes detection 24 hr after enrichment*
    • Listeria spp. detection 24 and 48 hr after enrichment
    • L. monocytogenes enumeration in 24 hr without confirmation**
    • L. monocytogenes confirmation in 24 hr after using the iQ-Check Listeria monocytogenes II Kit
  • Simple and Cost Effective
    • Only 1 broth, and 1 plate required for each sample
    • Easy confirmation
  • Easy-to-Read Chromogenic Reaction
    • L. monocytogenes (PIPLC+/xylose-) forms blue colonies
    • L. ivanovii (PIPLC+/xylose+) formsblue-green colonies with distinct yellow halos
    • Other Listeria species form white colonies
  • Multipurpose Chromogenic Medium
    • RAPID'L.mono is the only chromogenic method to be validated for L. monocytogenes and Listeria spp. detection on the same plate
    • Can also be used for the enumeration or confirmation of L. monocytogenes
  • Validation
    • Certified NF VALIDATION according to the ISO 16140 standard and validated by AOAC

* Within the scope of ISO 16140 NordVal validation, no confirmation is required.

** Within the scope of the AFNOR validation, confirmation is not required when the presence of L. monocytogenes has been confirmed at the detection stage.

Principle

Reading and identification of L. monocytogenes are easy due to the unique chromogenic principle (PI-PLC activity) of RAPID'L.mono medium and the fermentation of a sugar (xylose)

  • L. monocytogenes (PIPLC+/xylose-) forms blue colonies
  • L. ivanovii (PIPLC+/xylose+) forms blue colonies with a yellow halo
  • Other Listeria spp. (PIPLC-/xylose+ or -) form white colonies with or without a yellow halo

RAPID'L.mono medium is very specific and inhibits the growth of other bacteria and yeasts.

The rhamnose test (< 6hr) and spot inoculation on Agar Listeria (AL agar) have been validated for confirmation of colonies grown on RAPID'L.mono medium.

Other Applications of RAPID'L.mono Medium

  • As a second selective medium in different standardized protocols (for example, the ISO 11290 reference method)
  • To confirm positive results obtained using the iQ-Check Listeria monocytogenes II PCR Detection Kit
  • To enumerate L. monocytogenes in 24 hr without confirmation

RAPID'L.mono Chromogenic Assay

Rapid l.mono medium
Enzymatic Activity l.mono cytogenes
L.
monocytogenes

L.
ivanovii

L.
innocua
l.mono medium welshimeri
L.
welshimeri
PI-PLC detection + + - -
Xylose fermentation - + - +

Ordering Information

RAPID'L.mono Medium is offered as ready-to-use plates (3563964) or as a dehydrated agar base (3564293).

When using the dehydrated broth base, RAPID'L.mono Supplement 1 (3564294) and RAPID'L.mono Supplement 2 (3564746) need to be added to the molten agar prior to pouring plates.

For convenience, RAPID'L.mono Medium is also available as a kit (3555294) comprising 190 ml of medium and one vial of each supplement.

Ordering

Ordering

items

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3555294
Pkg of 1, kit contains 190 ml of medium, 1 vial each of supplement 1 (3564294) and supplement 2 (3564746)
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3563694
Pkg of 20, 90 mm agar plates
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3563964
Pkg of 120, 90 mm agar plates
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3564293
Pkg of 1, 500 g of dehydrated agar base, enough for 7.4 L of medium
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3564294
Pkg of 10, vials of supplement 1 for 'RAPID´L.mono Agar, enough for 10 x 500 ml of medium
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3564746
Pkg of 10, vials of supplement 2, enough for 10 x 500 ml of medium
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3555797
Pkg of 6, 225 ml bottles
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3564604
Pkg of 1, 500 g of dehydrated broth base
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3564616
Pkg of 10, vials of 1/2 strength supplement for Fraser Broth, enough for 10 x 2.25 L of medium
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3578113
Pkg of 1 kit, includes lysis reagent, fluorescent probes, PCR amplification mix, positive and negative controls, for 94 samples
3555794
Pkg of 4, bags of medium, enough for 4 x 3 L
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3578124
Pkg of 1 kit, includes lysis reagent, fluorescent probes, PCR amplification mix, positive and negative controls, for 94 samples
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3555790
Pkg of 2, 5 L bags — for challenging and non-challenging enrichment conditions
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3554179
Pkg of 6, 225 ml bottles — for challenging and non-challenging enrichment conditions
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3555795
Pkg of 4, 3 L bags — for challenging and non-challenging enrichment conditions
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3564684
Pkg of 1, 500 g — for challenging and non-challenging enrichment conditions
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3564686
Pkg of 1, 5 kg — for challenging and non-challenging enrichment conditions
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12013260
Pkg of 5 L x 2 bags — for standard enrichment conditions
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12013259
Pkg of 1, 500 g — for standard enrichment conditions
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12013258
Pkg of 1, 5 kg — for standard enrichment conditions

Documents

Documents

Number Description Options
10000127436
RAPIDʹL.mono Agar, User Guide (English, French, German, Italian, Portuguese, Spanish)
FSD_16288
RAPID’Chromogenic Media Brochure
FSD_17933
Food Safety and Quality Reference Guide
7274
Complete Solutions for Service Laboratories
10000128740
Buffered Peptone Water Plus, User Guide (English, French, German, Italian, Portuguese, Spanish)
10000128738
Buffered Peptone Water Standard, User Guide (English, French, German, Italian, Portuguese, Spanish)