RAPID'L.mono Medium

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RAPID'<em>L.mono</em> Medium

Overview

Key Benefits
  • Fast
    • 24 hr incubation only for L. monocytogenes detection and enumeration
    • Easy-to-read chromogenic reaction
  • Simple and Affordable
    • Economical (1 broth, 1 plate)
    • Easy confirmation
  • Easy to Read
    • Blue colonies are L. monocytogenes
    • Blue-green colonies with distinct yellow halos are L. ivanovii
    • White colonies are other Listeria species
Protocol
Validation

Bio-Rad's solutions are validated by international certification bodies against standard methods, so the results you get are results you can trust.

 

AOAC

NF VALIDATION

NordVal

Health Canada
iQ-Check Real-Time PCR Methods
Campylobacter spp. Open in new window
Cronobacter spp. Open in new window
Enterobacteriaceae Open in new window
E.coli O157:H7 Open in new window Open in new window
Listeria monocytogenes Open in new window Open in new window Open in new window Open in new window
Listeria spp. Open in new window Open in new window Open in new window
Salmonella spp. Open in new window Open in new window Open in new window Open in new window
Salmonella Enteritidis Open in new window
Salmonella Typhimurium Open in new window
STEC 7 major O-groups Open in new window
STEC virulence genes Open in new window
Vibrio spp. Open in new window
RAPID'Chromogenic Methods
Bacillus cereus Open in new window
Campylobacter spp. Open in new window
Coliforms Open in new window Open in new window Open in new window Open in new window
Cronobacter spp. Open in new window
Enterobacteriaceae Open in new window
Escherichia coli Open in new window Open in new window Open in new window Open in new window
E. coli O157:H7 Open in new window Open in new window
Listeria monocytogenes

Detection
Enumeration
AL


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Listeria spp. Open in new window Open in new window
Salmonella spp. Open in new window Open in new window Open in new window
Staphylococcus aureus Open in new window

* Alternative analytical methods for agrifood — http://nf-validation.afnor.org/en

Selective chromogenic medium for the direct detection and enumeration of Listeria monocytogenes and Listeria spp. in food and environmental samples. 24 hr to results.
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Ordering Information


Description

Talk with a Specialist

RAPID'L.mono Medium is a selective chromogenic medium for the direct detection and enumeration of Listeria monocytogenes and Listeria spp. in food and environmental samples.

Key Benefits

  • Fast
    • L. monocytogenes detection 24 hr after enrichment*
    • Listeria spp. detection 24 and 48 hr after enrichment
    • L. monocytogenes enumeration in 24 hr without confirmation**
    • L. monocytogenes confirmation in 24 hr after using the iQ-Check Listeria monocytogenes II Kit
  • Simple and Cost Effective
    • Only 1 broth, and 1 plate required for each sample
    • Easy confirmation
  • Easy-to-Read Chromogenic Reaction
    • L. monocytogenes (PIPLC+/xylose-) forms blue colonies
    • L. ivanovii (PIPLC+/xylose+) formsblue-green colonies with distinct yellow halos
    • Other Listeria species form white colonies
  • Multipurpose Chromogenic Medium
    • RAPID'L.mono is the only chromogenic method to be validated for L. monocytogenes and Listeria spp. detection on the same plate
    • Can also be used for the enumeration or confirmation of L. monocytogenes
  • Validation
    • Certified NF VALIDATION according to the ISO 16140 standard and validated by AOAC

* Within the scope of ISO 16140 NordVal validation, no confirmation is required.

** Within the scope of the AFNOR validation, confirmation is not required when the presence of L. monocytogenes has been confirmed at the detection stage.

Principle

Reading and identification of L. monocytogenes are easy due to the unique chromogenic principle (PI-PLC activity) of RAPID'L.mono medium and the fermentation of a sugar (xylose)

  • L. monocytogenes (PIPLC+/xylose-) forms blue colonies
  • L. ivanovii (PIPLC+/xylose+) forms blue colonies with a yellow halo
  • Other Listeria spp. (PIPLC-/xylose+ or -) form white colonies with or without a yellow halo

RAPID'L.mono medium is very specific and inhibits the growth of other bacteria and yeasts.

The rhamnose test (< 6hr) and spot inoculation on Agar Listeria (AL agar) have been validated for confirmation of colonies grown on RAPID'L.mono medium.

Other Applications of RAPID'L.mono Medium

  • As a second selective medium in different standardized protocols (for example, the ISO 11290 reference method)
  • To confirm positive results obtained using the iQ-Check Listeria monocytogenes II PCR Detection Kit
  • To enumerate L. monocytogenes in 24 hr without confirmation

RAPID'L.mono Chromogenic Assay

Rapid l.mono medium
Enzymatic Activity l.mono cytogenes
L.
monocytogenes

L.
ivanovii

L.
innocua
l.mono medium welshimeri
L.
welshimeri
PI-PLC detection + + - -
Xylose fermentation - + - +

Ordering Information

RAPID'L.mono Medium is offered as ready-to-use plates (3563964) or as a dehydrated agar base (3564293).

When using the dehydrated broth base, RAPID'L.mono Supplement 1 (3564294) and RAPID'L.mono Supplement 2 (3564746) need to be added to the molten agar prior to pouring plates.

For convenience, RAPID'L.mono Medium is also available as a kit (3555294) comprising 190 ml of medium and one vial of each supplement.


Specifications

Assay type Chromogenic media
Organism Listeria monocytogenes, Listeria spp.

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RAPID'L.mono Agar Medium with Supplements

3555294
Pkg of 1, kit contains 190 ml of medium, 1 vial each of supplement 1 (3564294) and supplement 2 (3564746)

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$59.00 
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RAPID'L.mono Agar Plates

3563694
Pkg of 20, 90 mm agar plates

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$78.00 
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RAPID'L.mono Agar Plates

3563964
Pkg of 120, 90 mm agar plates

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$565.00 
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RAPID´L.mono Agar, dehydrated

3564293
Pkg of 1, 500 g of dehydrated agar base, enough for 7.4 L of medium

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$655.00 
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RAPID´L.mono Supplement 1

3564294
Pkg of 10, vials of supplement 1 for 'RAPID´L.mono Agar, enough for 10 x 500 ml of medium

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$320.00 
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RAPID'L.mono and RAPID'Listeria spp. Supplement 2

3564746
Pkg of 10, vials of supplement 2, enough for 10 x 500 ml of medium

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$139.00 
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Fraser 1/2 Broth, ready to use

3555797
Pkg of 6, 225 ml bottles

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$44.00 
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Fraser Broth Base, dehydrated

3564604
Pkg of 1, 500 g of dehydrated broth base

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$117.00 
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Fraser 1/2 Strength Supplement

3564616
Pkg of 10, vials of 1/2 strength supplement for Fraser Broth, enough for 10 x 2.25 L of medium

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$203.00 
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iQ-Check Listeria spp. PCR Detection Kit

3578113
Pkg of 1 kit, includes lysis reagent, fluorescent probes, PCR amplification mix, positive and negative controls, for 94 samples

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Fraser 1/2 Broth, ready to use

3555794
Pkg of 4, bags of medium, enough for 4 x 3 L

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$223.00 
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iQ-Check Listeria monocytogenes II PCR Detection Kit

3578124
Pkg of 1 kit, includes lysis reagent, fluorescent probes, PCR amplification mix, positive and negative controls, for 94 samples

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Buffered Peptone Water Plus

3555790
Pkg of 2, 5 L bags — for challenging and non-challenging enrichment conditions

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$113.00 
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Buffered Peptone Water Plus

3554179
Pkg of 6, 225 ml bottles — for challenging and non-challenging enrichment conditions

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$28.00 
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Buffered Peptone Water Plus

3555795
Pkg of 4, 3 L bags — for challenging and non-challenging enrichment conditions

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$114.00 
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Buffered Peptone Water Plus

3564684
Pkg of 1, 500 g — for challenging and non-challenging enrichment conditions

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$78.00 
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Buffered Peptone Water Plus

3564686
Pkg of 1, 5 kg — for challenging and non-challenging enrichment conditions

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$716.00 
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Buffered Peptone Water Standard

12013260
Pkg of 5 L x 2 bags — for standard enrichment conditions

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$99.00 
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Buffered Peptone Water Standard, dehydrated

12013259
Pkg of 1, 500 g — for standard enrichment conditions

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$68.00 
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Buffered Peptone Water Standard, dehydrated

12013258
Pkg of 1, 5 kg — for standard enrichment conditions

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$622.00 
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Documents

Number Description Options
7213EN Technical Sheet, RAPID'L.mono Agar, Ver 14 Click to download
7213FR Fiche Technique, RAPID'L.mono Gelose, Ver 14 Click to download
FSD_16288 RAPID’Chromogenic Media Brochure Click to download
FSD_17933 Food Safety and Quality Reference Guide Click to download
7274 Complete Solutions for Service Laboratories Click to download
10000128740 Buffered Peptone Water Plus, User Guide (English, French, German, Italian, Portuguese, Spanish) Click to download
10000128738 Buffered Peptone Water Standard, User Guide (English, French, German, Italian, Portuguese, Spanish) Click to download

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