iQ-Check Listeria monocytogenes II PCR Detection Kit

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Overview

Complete sample preparation and PCR kit for the rapid detection of Listeria monocytogenes in food and environmental samples. Next-day results.

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Description

Description

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The iQ-Check Listeria monocytogenes II Kit is based on the fast, sensitive, and proven technology of real-time PCR. It is intended for detecting Listeria monocytogenes in environmental samples and in food samples such as dairy products and fish.

Due to the sensitivity and specificity of PCR, results can be obtained in as little as 24 hr after a single enrichment in a selective medium.

Highly Specific and Reliable

The iQ-Check Listeria monocytogenes II Kit uses an optimized system of primers and probes to ensure high specificity and eliminate cross-reactions. It is designed as a multiplex reaction that includes an internal inhibition control that is amplified in parallel with the target DNA for a reliable result.

Maximum Flexibility

Developed as an open and flexible system, the iQ-Check Listeria monocytogenes II Kit can be used for up to 94 samples. Reactions can be run on low- or high-throughput Bio-Rad instruments, depending on the laboratory’s needs. Different iQ-Check tests can also be run in parallel.

Validated Protocols

The iQ-Check Listeria monocytogenes II Kit protocol has been validated by several certification bodies. These validation studies have shown that the kit is equivalent to or better than the various reference methods evaluated and provides results in much less time.

A Complete Solution

Bio-Rad offers a complete solution with standardized reagents, including enrichment media, PCR kits, confirmation agars, and PCR detection systems, in addition to software for automated analysis specific to each test.

 Kit Components

  • Lysis reagent for DNA extraction
  • Fluorescent probes
  • PCR amplification mix
  • Positive and negative PCR controls

Related Products

Specifications

Specifications

Ordering

Ordering

items

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3578124
Pkg of 1 kit, includes lysis reagent, fluorescent probes, PCR amplification mix, positive and negative controls, for 94 samples
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3594970
Pkg of 1, 1 vial of lyophilized iQ-Check Free DNA Removal Reagent (to make 10 ml) and 1 vial of iQ-Check 10x Activation Buffer (9 ml)
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12012383
Pkg of 1, vial of Purification Reagent (to make 25 ml), for 96 tests
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3555703
Pkg of 6, 225 ml bottles of liquid media
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3564703
Pkg of 1, 500 g of dehydated broth
3555794
Pkg of 4, bags of medium, enough for 4 x 3 L
3564753
Pkg of 1, 5 kg of dehydated broth

Accessories

Accessories

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3600040
Pkg of 100, PCR plate sealing film, pre-pierced
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MSB1001XTU
Pkg of 100, optically clear seal for PCR plates for real-time PCR
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2240110XTU
Pkg of 500, screwcap micro test tube, sterilized, with installed O-ring screwcaps
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TLS0851XTU
Pkg of 120, thin-wall polypropylene 8–tube strips for PCR and low volume reactions, white, (960 tubes)
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TCS0803XTU
Pkg of 120, 8-cap strips for 0.2 ml tubes and plates for PCR and optical reactions, ultraclear (960 caps)
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MLL4851XTU
Pkg of 50, white PCR plate, 8 x 6 format, one-component polypropylene, can be cut for fewer samples
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MLL9651XTU
Pkg of 25, white PCR plate, one-component polypropylene, can be cut for fewer samples
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ECT2000
Pkg of 1, tool for seating 8- and 12-cap strips on PCR plates or tubes
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HSP9655XTU
Pkg of 50, white shell/white well PCR plate for higher fluorescent signals, rigid 2-component design
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HSP9955XTU
Pkg of 50, white shell/white well PCR plate for higher fluorescent signals, barcoded, 7-digit sequential, Code 128, rigid 2-component design
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3594900
Pkg of 50, 96-well plates plates, deep well
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(Discontinued) X-Pierce Films
3593977
Pkg of 100, PCR plate sealing film, pre-pierced

Equipment

Equipment

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3600037

Modular thermal cycler platform, includes C1000 Touch Thermal Cycler chassis, CFX96 Deep Well Optical Reaction Module, cables. To be used with CFX Manager Software, Industrial Diagnostic Edition.

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3591456
Pkg of 1, cell disruption device, for DNA extraction for iQ-Check® PCR test Detection Kits
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(Discontinued) iQ-Check Prep System
3594911
Automated system for DNA extraction and real-time PCR plate set-up, 1 unit
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(Discontinued) Heating Thermoshaker
3594995
Pkg of 1, temperature-controlled shaker for cell lysis, for use with iQ-Check PCR Detection Kits
3594996
Pkg of 1, deep well block for thermoshaker
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Documents

Documents

Number Description Options
10000123535
iQ-Check Listeria monocytogenes II Kit User Guide (English, French, German, Italian, Portuguese, Spanish)
FSD_16439
iQ-Check Real-Time PCR Solution Brochure
7401
Performance summary, iQ-Check Listeria monocytogenes
6960
iQ-Check Prep Solution Brochure
7117
iQ-Check Listeria spp kit Quick Guide: Easy II Extraction Deep Well Protocol Using the Thermoshaker
3240
iQ-Check PCR Kits Easy I and II Extraction TUBE Protocol Quick Guide
10000136002
Listeria Special Broth (LSB), User Guide (English, French, German, Italian, Portuguese, Spanish)
10000058391
iQ-Check Free DNA Removal Solution Product Insert
7322
iQ-Check Free DNA Removal Solution — Quick Guide
6834
iQ-Check Free DNA Removal Solution Brochure
10000119734
iQ-Check Purification Reagent Technical sheet
FSD_17933
Food Safety and Quality Reference Guide
7274
Complete Solutions for Service Laboratories