
Overview
The iQ-Check Listeria monocytogenes II Kit is based on the fast, sensitive, and proven technology of real-time PCR. It is intended for detecting Listeria monocytogenes in environmental samples and in food samples such as dairy products and fish.
Due to the sensitivity and specificity of PCR, results can be obtained in as little as 24 hr after a single enrichment in a selective medium.
Highly Specific and Reliable
The iQ-Check Listeria monocytogenes II Kit uses an optimized system of primers and probes to ensure high specificity and eliminate cross-reactions. It is designed as a multiplex reaction that includes an internal inhibition control that is amplified in parallel with the target DNA for a reliable result.
Maximum Flexibility
Developed as an open and flexible system, the iQ-Check Listeria monocytogenes II Kit can be used for up to 94 samples. Reactions can be run on low- or high-throughput Bio-Rad instruments, depending on the laboratory’s needs. Different iQ-Check tests can also be run in parallel.
Validated Protocols
The iQ-Check Listeria monocytogenes II Kit protocol has been validated by several certification bodies. These validation studies have shown that the kit is equivalent to or better than the various reference methods evaluated and provides results in much less time.
A Complete Solution
Bio-Rad offers a complete solution with standardized reagents, including enrichment media, PCR kits, confirmation agars, and PCR detection systems, in addition to software for automated analysis specific to each test.
Kit Components
- Lysis reagent for DNA extraction
- Fluorescent probes
- PCR amplification mix
- Positive and negative PCR controls
Specifications
Ordering
items
Use the filters below to refine results!


Pkg of 1, 1 vial of lyophilized iQ-Check Free DNA Removal Reagent (to make 10 ml) and 1 vial of iQ-Check 10x Activation Buffer (9 ml)

Pkg of 1, vial of Purification Reagent (to make 25 ml), for 96 tests


Pkg of 6, 225 ml bottles of liquid media
Pkg of 1, 500 g of dehydrated broth
Pkg of 1, 5 kg of dehydrated broth

2 bags, each containing 5 L of ready-to-use Listeria Special Broth II
Pkg of 4, bags of medium, enough for 4 x 3 L
Accessories










Pkg of 100, optically clear seal for PCR plates for real-time PCR

Pkg of 500, screwcap micro test tube, sterilized, with installed O-ring screwcaps

Pkg of 960, 20–200 µl pipet tip with aerosol barrier, racked, certified free of pyrogen, DNase, RNase; For food application only

Pkg of 1,000, 100–1,000 µl pipet tip with aerosol barrier, bulk, certified free of pyrogen, DNase, RNase, For food application only

Pkg of 960, 100–1,000 µl pipet tip with aerosol barrier, racked, certified free of pyrogen, DNase, RNase, For food application only

Pkg of 960, 20–200 µl pipet tip with aerosol barrier, racked, certified free of pyrogen, DNase, RNase; For food application only
Equipment

Modular thermal cycler platform, includes C1000 Touch Thermal Cycler chassis, CFX96 Deep Well Optical Reaction Module, cables. To be used with CFX Manager Software, Industrial Diagnostic Edition.

96-well, 5-color plus FRET, network-connected real-time PCR detection system, includes CFX Opus 96 base unit, cables

96-well, 5-color plus FRET, network-connected real-time PCR detection system, includes CFX Opus 96 base unit, and cables
Pkg of 1, cell disruption device, for DNA extraction for iQ-Check PCR test Detection Kits



CFX Manager Software, Industrial Diagnostic Edition