Criterion™ Tris-Tricine Precast Gels

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Criterion Tris-Tricine Precast Gels

Overview

Criterion Tris-Tricine Precast Gels

The banding patterns indicate the optimal separation ranges (in kD) for each acrylamide percentage in combinations with the specified buffer system.

Tris-Tricine gels are ideal for separation of peptides and small proteins with a molecular weight <10,000. Superior resolution is achieved by slowing the migration rate of the peptide-SDS complexes.
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Ordering Information


Description

Criterion™ Tris-Tricine precast gels are ideal for vertical midi gel electrophoresis of peptides and small proteins with a molecular weight <10,000 D. Superior resolution is achieved by slowing the migration rate of the peptide-SDS complexes. This helps achieve separation from faster-moving SDS micelles that interfere with peptide resolution in Tris-glycine buffer systems.

Features and Benefits

  • Ideal for the separation of small molecular–weight proteins
  • Reproducible, high-resolution results
  • Fast setup, loading, and run times
  • Numbered, marked well outlines on the cassette ease sample loading and identification
  • Run 2-D gels in less than a day
  • Robust system allows separation of complex samples

Applications and Uses

  • Polyacrylamide gel electrophoresis (PAGE or SDS-PAGE)
  • 2-D gel electrophoresis
  • Accurate molecular weight estimation

Use the Protein Migration Chart to select a gel percentage that will provide optimal resolution of your sample.


Specifications

  Gel dimensions (W x L x thickness), cm 13.3 x 8.7 x 0.1
  Cassette dimensions (W x L x thickness), cm 15.0 x 10.6 x .53
  Cassette material Styrene copolymer
Comb material Polycarbonate
Storage tray material PET
Gel storage conditions Store flat at 2–8°C; do not freeze
  Gel shelf life at recommended temperature* 12 weeks
  Buffer volume, ml Upper, 60; lower, 400
Recommended sample buffer Tricine Sample Buffer (1610739); dilute at least 1:1 with sample
  Gel chemistry Tris-HCl
  Gel format Midi
* From date of manufacture.  

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16.5% Criterion™ Tris-Tricine Gel, 12+2 well, 45 µl

3450063
Pkg of 1, 16.5% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

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$16.00 
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16.5% Criterion™ Tris-Tricine Gel, 18 well, 30 µl

3450064
Pkg of 1, 16.5% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

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$16.00 
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16.5% Criterion™ Tris-Tricine Gel, 26 well, 15 µl

3450065
Pkg of 1, 16.5% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

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$16.00 
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(Discontinued) 16.5% Criterion™ Tris-Tricine Gel, Prep+2 well, 700 µl

3450066
Pkg of 1, 16.5% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells

This product is no longer available. Please contact technical support for more information on replacement product.
10–20% Criterion™ Tris-Tricine Gel, 12+2 well, 45 µl

3450067
Discontinued item
Replaced with 16.5% Criterion Tris-Tricine Gel, 12+2 well, 45 µl (3450063)

This product is being discontinued soon. Please contact customer service for more information.
10–20% Criterion™ Tris-Tricine Gel, 18 well, 30 µl

3450068
Discontinued item
Replaced with 16.5% Criterion Tris-Tricine Gel, 18-well, 30 µl (3450064)

This product is being discontinued soon. Please contact customer service for more information.
10–20% Criterion™ Tris-Tricine Gel, 26 well, 15 µl

3450069
Discontinued item
Replaced with 16.5% Criterion Tris-Tricine Gel, 26-well, 15 µl (3450065)

This product is being discontinued soon. Please contact customer service for more information.

Accessories

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Mini-PROTEAN® Cassette Opening Lever

4560000
Pkg of 1, 100% aluminum and recyclable opening lever for Mini-PROTEAN precast gel cassettes

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$5.00 
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Kits & Reagents

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10x Tris/Tricine/SDS Running Buffer, 1 L

1610744
Pkg of 1, 1 L, 10x premixed electrophoresis buffer, contains 100 mM Tris, 100 mM Tricine, 0.1% SDS, pH 8.3 following dilution to 1x with water

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$100.00 
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Tricine Sample Buffer for Protein Gels, 30 ml

1610739
30 ml, premixed protein sample buffer for peptide and small protein SDS-PAGE, contains 200 mM Tris-HCl, pH 6.8, 40% glycerol, 2% SDS, 0.04% Coomassie Blue G-250

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$14.00 
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Dithiothreitol (DTT)

1610610
1 g, dithiothreitol (DTT) reducing agent

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$31.00 
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Dithiothreitol (DTT)

1610611
5 g, dithiothreitol (DTT) reducing agent

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$94.00 
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2-Mercaptoethanol

1610710
25 ml, ≥98% pure 2-mercaptoethanol (14.2 M)

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$27.00 
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Documents

Number Description Options
2788 Criterion Blotting Tray Insert, Rev A Click to download
2710 Criterion™ Precast Gel System Brochure, Rev C Click to download
4110001 Instruction Manual, Criterion™ Gel Application Guide, Ver H Click to download

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