Description
Description
Criterion™ Tris-Tricine precast gels are ideal for vertical midi gel electrophoresis of peptides and small proteins with a molecular weight <10,000 D. Superior resolution is achieved by slowing the migration rate of the peptide-SDS complexes. This helps achieve separation from faster-moving SDS micelles that interfere with peptide resolution in Tris-glycine buffer systems.
Features and Benefits
- Ideal for the separation of small molecular–weight proteins
- Reproducible, high-resolution results
- Fast setup, loading, and run times
- Numbered, marked well outlines on the cassette ease sample loading and identification
- Run 2-D gels in less than a day
- Robust system allows separation of complex samples
Applications and Uses
- Polyacrylamide gel electrophoresis (PAGE or SDS-PAGE)
- 2-D gel electrophoresis
- Accurate molecular weight estimation
Use the Protein Migration Chart to select a gel percentage that will provide optimal resolution of your sample.
Specifications
Specifications
Gel dimensions (W x L x thickness), cm
13.3 x 8.7 x 0.1
Cassette dimensions (W x L x thickness), cm
15.0 x 10.6 x .53
Cassette material
Styrene copolymer
Comb material
Polycarbonate
Storage tray material
PET
Gel storage conditions
Store flat at 2–8°C; do not freeze
Gel shelf life at recommended temperature*
12 weeks
Buffer volume, ml
Upper, 60; lower, 400
Recommended sample buffer
Tricine Sample Buffer (1610739); dilute at least 1:1 with sample
Gel chemistry
Tris-HCl
* From date of manufacture.
Ordering
Ordering
items
Use the filters below to refine results!
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(Discontinued) 16.5% Criterion™ Tris-Tricine Gel, 12+2 well, 45 µl
3450063
Pkg of 1, 16.5% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells
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(Discontinued) 16.5% Criterion™ Tris-Tricine Gel, 18 well, 30 µl
3450064
Pkg of 1, 16.5% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells
Image
(Discontinued) 16.5% Criterion™ Tris-Tricine Gel, 26 well, 15 µl
3450065
Pkg of 1, 16.5% precast polyacrylamide gel, 13.3 × 8.7 cm (W × L), for use with Criterion and Criterion™ Dodeca™ electrophoresis cells
Accessories
Accessories
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(Discontinued) Mini-PROTEAN® Cassette Opening Lever
4560000
Pkg of 1, 100% aluminum and recyclable opening lever for Mini-PROTEAN precast gel cassettes
Kits & Reagents
Kits & Reagents
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(Discontinued) 10x Tris/Tricine/SDS Running Buffer, 1 L
1610744
Pkg of 1, 1 L, 10x premixed electrophoresis buffer, contains 100 mM Tris, 100 mM Tricine, 0.1% SDS, pH 8.3 following dilution to 1x with water
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(Discontinued) Tricine Sample Buffer for Protein Gels, 30 ml
1610739
30 ml, premixed protein sample buffer for peptide and small protein SDS-PAGE, contains 200 mM Tris-HCl, pH 6.8, 40% glycerol, 2% SDS, 0.04% Coomassie Blue G-250
Image
(Discontinued) Dithiothreitol (DTT)
1610610
1 g, dithiothreitol (DTT) reducing agent
Image
(Discontinued) Dithiothreitol (DTT)
1610611
5 g, dithiothreitol (DTT) reducing agent
Image
(Discontinued) 2-Mercaptoethanol
1610710
25 ml, ≥98% pure 2-mercaptoethanol (14.2 M)
Documents
Documents
Login Required Number Description Options
2788
Criterion Blotting Tray Insert, Rev A
2710
Criterion™ Precast Gel System Brochure, Rev C
4110001
Instruction Manual, Criterion™ Gel Application Guide, Ver H