Clarity and Clarity Max ECL Western Blotting Substrates

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ECL Substrates for High-Sensitivity Western Blot Detection

Chemiluminescent assays depend on the emission of light as a product of a chemical reaction and are commonly used for the detection of proteins on western blots. The secondary antibody used in western blotting is conjugated to the horseradish peroxidase (HRP) enzyme which reacts with the HRP substrate luminol. This reaction emits light at 428 nm and this light signal can be captured on X-ray film or by a digital imager.

However, luminol emits light only weakly, so enhancers are added to the reaction to increase the signal. This enhancement of a luminol-based signal is commonly referred to as enhanced chemiluminescence (ECL).

There are a number of different enhancers available, some of which can increase the signal by as much as a 1,000-fold, making ECL more sensitive than other common detection systems such as conversion of substrates to colored precipitates. For example, the enhancers used in Bio-Rad Clarity Max ECL substrate maximizes detection sensitivity to enable detection to the low femtogram amounts of target. The type of enhancer used can also increase the signal duration. Luminol has an emission half-life of less than a minute. The Bio-Rad Clarity ECL substrate extends the signal duration to 24 hours, permitting reimaging without loss of signal intensity.

Clarity Family of ECL Western Blotting Substrates

Enhanced chemiluminescence (ECL) is a highly sensitive method for detecting proteins bound to western blotting membranes. Bio-Rad's Clarity family of ECL substrates provides simple, high-performance solutions for all your western blotting needs.

Clarity ECL Substrate is our most versatile chemiluminescence substrate yielding high signal, low background and a long signal duration. Clarity Max ECL Substrate is the substrate of choice for challenging blots that require maximum levels of sensitivity. Both substrates are compatible with any horseradish peroxidase (HRP) conjugate and ideal for all western blots and both digital and film-based imaging. Substrate components are stable at room temperature.

Clarity Western ECL Substrate

Use Clarity Western ECL Substrate with any HRP-conjugated secondary antibody for digital or film-based imaging of western blots.

Features and Benefits

• Mid-femtogram-level sensitivity
• 24-hour signal duration
• 1-year shelf-life at room temperature
• Ideal for digital and film-based imaging

Clarity ECL Substrate exhibits excellent visual limit of detection versus other commonly used ECL substrates on the market.

View Clarity Substrate performance data

Clarity Max Western ECL Substrate

Use Clarity Max Western ECL Substrate with any HRP-conjugated secondary antibody for digital or film-based imaging of any western blots.

Features and Benefits

• Low-femtogram-level sensitivity
• Recommended for detection of low target amounts
• Ideal for digital and film-based imaging

Clarity Max ECL Substrate exhibits superior visual limit of detection versus other commonly used high-performance ECL substrates on the market.

View Clarity Max Substrate performance data

Packaging Options

Companion Products

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  Western Blotting

  Western blotting products include the V3 Western Workflow, protein transfer systems, membranes, filter paper, blotting buffers, and detection kits

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  Protein Gels

  Choose SDS-PAGE and native PAGE gels, convert to TGX precast gels, or choose specialized gel chemistries including Stain-Free

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  Imaging Systems & Software

  Detect, image and quantify multiplex fluorescence, chemiluminescence, stain-free and colorimetric blots and gels.