Clarity™ Western ECL Substrate, 20 ml #1705060S
Pkg of 1, contains 10 ml Clarity Western Peroxide Reagent and 10 ml Clarity Western Luminol/Enhancer Reagent
Available Safety Data Sheets
Safety Data Sheet
Overview
Use Clarity Western ECL Substrate with any HRP-conjugated secondary antibody for digital or film-based imaging.
Features and Benefits
- Femtogram-level sensitivity
- 24-hr signal duration
- 1-year shelf-life at room temperature
- Use 7 ml for a mini membrane (7 x 8.5 cm)
- Use 12 ml for a midi membrane (8.5 x 13.5 cm)
Product Contents
- 10 ml Clarity Western Peroxide Reagent
- 10 ml Clarity Western Luminol/Enhancer Reagent
Packaging Options
| Number of applications | Total Volume | Catalog Number |
| 1 to 3 membranes | 20 ml | 1705060S |
| 16 to 28 membranes | 200 ml | 1705060 |
| 32 to 56 membranes | 500 ml | 1705061 |
Related Products
- Clarity Max Western ECL Substrate, 20 ml (1705062S)
- Clarity Max Western ECL Substrate, 100 ml (1705062)
- Immun-Star™ Goat Anti-Mouse (GAM)-HRP Conjugate, 2 ml (1705047)
- Immun-Star Goat Anti-Rabbit (GAR)-HRP Conjugate, 2 ml (1705046)
- Premixed Transfer Buffers
- Transfer Buffer Reagents
- Detergents and Blocking Buffers
Related Categories
- Western Blotting Membranes
- Immunodetection Reagents and Kits
- Western Blotting Products
- Protein Electrophoresis and Blotting
Supporting Documents
- Blotting Membrane and Filter Paper Selection Guide
- Clarity Western ECL Substrate Quick Start Guide
- Protein Blotting Guide
Online Resources
- Information on Membranes and Blotting Papers
- Basic principles of Protein Blotting Methods
- Introduction to Western Blotting
Specifications
Lower detection limit
Clarity: Mid-femtogram level
Clarity Max: Low-femtogram level
Clarity Max: Low-femtogram level
Signal duration
Clarity: 24 hr
Clarity Max: 2 hr
Clarity Max: 2 hr
Primary detection method
Digital or film imaging
Suggested antibody dilution
Primary: 1:1,000–1:50,000;
Secondary: 1:50,000–1:250,000
Secondary: 1:50,000–1:250,000
Recommended membrane
Nitrocellulose, PVDF, or LF PVDF
Recommended Products
Image
(Discontinued) Clarity Max Western ECL Substrate, 100 ml
1705062
Pkg of 1, contains 50 ml Clarity Max Western Peroxide Reagent and 50 ml Clarity Max Western Luminol/Enhancer Reagent
Image
(Discontinued) Immun-Star Goat Anti-Mouse (GAM)-HRP Conjugate
1705047
2 ml, horseradish peroxidase secondary antibody conjugate, for use with all chemiluminescent substrate kits
Image
(Discontinued) Immun-Star Goat Anti-Rabbit (GAR)-HRP Conjugate
1705046
2 ml, horseradish peroxidase secondary antibody conjugate, for use with all chemiluminescent substrate kits