Overview
Chemiluminescence and colorimetric detection kits for western blot applications are available in both AP and HRP formats. Bio-Rad's chemiluminescent detection products are optimized for use with CCD imagers.
Category Products
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Clarity and Clarity Max ECL Western Blotting SubstratesClarity Western ECL Blotting Substrate is compatible with any HRP-conjugate secondary antibody and ideal for both digital and film-based imaging.
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Immun-Star™ AP Chemiluminescence Kits
The highly sensitive Immun-Star AP kit combines Bio-Rad's blotting reagents and expertise with CDP-Star chemiluminescence technology.
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Immun-Blot® Colorimetric Kits
Bio-Rad's HRP-based colorimetric detection systems offer a choice of HRP-conjugated secondary antibodies and HRP-conjugated protein A or protein G using the detection reagent 4-chloro-1-naphthol II (4CN).
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Immun-Blot® AP Colorimetric KitsAP-based colorimetric kits utilize 5-bromo-4-chloro-3-indolyl phosphate/Nitroblue Tetrazolium (BCIP/NBT), which reacts with AP to form a color precipitate. Can detect as little as 100 pg of protein.
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HRP and AP Conjugates
The high titer of the blotting-grade antibody conjugates increase assay sensitivity. Greater working dilutions (1:3,000) decrease background and increase the signal-to-noise ratio of the assay
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Detergents and Blocking Reagents for BlottingPremixed blocking buffers available are PBS/casein and TBS/casein. Pipetting of Tween 20 is accurate and easy with 10% Tween 20.
