HRP and AP Conjugates
The high titer of the blotting-grade antibody conjugates increase assay sensitivity. Greater working dilutions (1:3,000) decrease background and increase the signal-to-noise ratio of the assay
Overview
Blotting-grade HRP conjugates (horseradish peroxidase) and AP conjugates (alkaline phosphatase) produce specific results, eliminating false positives in western blotting immunoassays
- Double affinity-purified blotting-grade antibodies are isolated by affinity chromatography and further purified by cross-adsorption against an unrelated species to eliminate nonspecific immunoglobulins
- The high titer of the blotting-grade antibody conjugates increase assay sensitivity. Greater working dilutions (1:3,000) decrease background and increase the signal-to-noise ratio of the conjugated enzyme assay
Ordering
items
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(Discontinued) Goat Anti-Rabbit IgG (H + L)-HRP Conjugate
1706515
2 ml, blotting-grade horseradish peroxidase secondary antibody conjugate
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(Discontinued) Goat Anti-Mouse IgG (H + L)-HRP Conjugate
1706516
2 ml, blotting-grade horseradish peroxidase secondary antibody conjugate
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(Discontinued) Goat Anti-Human (H+L) HRP conjugate
1721050
2 ml, EIA-grade, enzyme-antibody conjugate
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(Discontinued) Avidin-HRP
1706528
2 ml, HRP-Avidin conjugate for detection of biotinylated SDS-PAGE standards
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(Discontinued) Immun-Star Goat Anti-Mouse (GAM)-HRP Conjugate
1705047
2 ml, horseradish peroxidase secondary antibody conjugate, for use with all chemiluminescent substrate kits
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(Discontinued) Immun-Star Goat Anti-Rabbit (GAR)-HRP Conjugate
1705046
2 ml, horseradish peroxidase secondary antibody conjugate, for use with all chemiluminescent substrate kits
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(Discontinued) Goat Anti-Rabbit IgG-AP Conjugate
1706518
1 ml, blotting-grade alkaline phosphatase secondary antibody conjugate
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(Discontinued) Goat Anti-Mouse IgG-AP Conjugate
1706520
1 ml, blotting-grade alkaline phosphatase secondary antibody conjugate
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(Discontinued) Goat Anti-Human IgG-AP Conjugate
1706521
1 ml, blotting-grade alkaline phosphatase secondary antibody conjugate
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(Discontinued) Avidin-AP
1706533
1 ml, blotting-grade alkaline phosphatase (AP) avidin conjugate
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(Discontinued) Blotting-Grade Biotinylated AP Conjugate
1706403
1 ml, alkaline phosphatase (AP) secondary antibody conjugate
Documents
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Number Description Options
LIT423
Instruction Manual, Blotting-Grade Avidin-Horseradish Peroxidase Conjugate, Rev E
5576
Molecular Weight Estimation and Quantitation of Protein Samples Using Precision Plus Protein WesternC Standards, the Immun-Star WesternC Chemiluminescent Detection Kit, and the Molecular Imager ChemiDoc XRS Imaging System, Rev C
LIT417
Instruction Manual, Blotting Grade Protein G - Horseradish Peroxidate Conjugate, Rev C
LIT422
Instruction Manual, Blotting Grade Protein A- Horseradish Peroxidase Conjugate
LIT418
Instruction Manual, Blotting Grade Goat Anti-Rabbit IgG (H + L) (Human IgG Adsorbed) Horseradish Peroxidase Conjugate, Rev F
LIT419
Instruction Manual, Blotting Grade Affinity Purified Goat Anti-Rabbit IgG (H + L) Alkaline Phosphatase Conjugate
LIT372
Instruction Manual, Blotting Grade Affinity Purified Goat Anti-Mouse IgG (H + L) Horseradish Peroxidase Conjugate, Rev D
LIT420
Instruction Manual, Blotting Grade Affinity Purified Goat Anti-Mouse IgG (H + L) Alkaline Phosphatase Conjugate, Rev D
LIT421
Instruction Manual, Blotting Grade Affinity Purified Goat Anti-Human IgG (H + L) Alkaline Phosphatase Conjugate
LIT440
Instruction Manual, Blotting Grade Affinity Purified Goat Anti-Human IgG ((H + L) Horseradish Peroxidase Conjugate
2032
Western Blotting Detection Reagents Brochure, Rev F