HRP and AP Conjugates

Image
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Overview

The high titer of the blotting-grade antibody conjugates increase assay sensitivity. Greater working dilutions (1:3,000) decrease background and increase the signal-to-noise ratio of the assay
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Description

Description

Blotting-grade HRP conjugates (horseradish peroxidase) and AP conjugates (alkaline phosphatase) produce specific results, eliminating false positives in western blotting immunoassays

  • Double affinity-purified blotting-grade antibodies are isolated by affinity chromatography and further purified by cross-adsorption against an unrelated species to eliminate nonspecific immunoglobulins
  • The high titer of the blotting-grade antibody conjugates increase assay sensitivity. Greater working dilutions (1:3,000) decrease background and increase the signal-to-noise ratio of the conjugated enzyme assay

Ordering

Ordering

items

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1706515
2 ml, blotting-grade horseradish peroxidase secondary antibody conjugate
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1706516
2 ml, blotting-grade horseradish peroxidase secondary antibody conjugate
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1721050
2 ml, EIA-grade, enzyme-antibody conjugate
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1706522
1 ml, blotting-grade protein A horseradish peroxidase antibody conjugate
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1706425
1 ml, blotting-grade protein G horseradish peroxidase antibody conjugate
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(Discontinued) Avidin-HRP
1706528
2 ml, HRP-Avidin conjugate for detection of biotinylated SDS-PAGE standards
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1705047
2 ml, horseradish peroxidase secondary antibody conjugate, for use with all chemiluminescent substrate kits
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1705046
2 ml, horseradish peroxidase secondary antibody conjugate, for use with all chemiluminescent substrate kits
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1706518
1 ml, blotting-grade alkaline phosphatase secondary antibody conjugate
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1706520
1 ml, blotting-grade alkaline phosphatase secondary antibody conjugate
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1706521
1 ml, blotting-grade alkaline phosphatase secondary antibody conjugate
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(Discontinued) Avidin-AP
1706533
1 ml, blotting-grade alkaline phosphatase (AP) avidin conjugate
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1706403
1 ml, alkaline phosphatase (AP) secondary antibody conjugate

Documents

Documents

Number Description Options
LIT423
Instruction Manual, Blotting-Grade Avidin-Horseradish Peroxidase Conjugate, Rev E
5576
Molecular Weight Estimation and Quantitation of Protein Samples Using Precision Plus Protein WesternC Standards, the Immun-Star WesternC Chemiluminescent Detection Kit, and the Molecular Imager ChemiDoc XRS Imaging System, Rev C
LIT417
Instruction Manual, Blotting Grade Protein G - Horseradish Peroxidate Conjugate, Rev C
LIT422
Instruction Manual, Blotting Grade Protein A- Horseradish Peroxidase Conjugate
LIT418
Instruction Manual, Blotting Grade Goat Anti-Rabbit IgG (H + L) (Human IgG Adsorbed) Horseradish Peroxidase Conjugate, Rev F
LIT419
Instruction Manual, Blotting Grade Affinity Purified Goat Anti-Rabbit IgG (H + L) Alkaline Phosphatase Conjugate
LIT372
Instruction Manual, Blotting Grade Affinity Purified Goat Anti-Mouse IgG (H + L) Horseradish Peroxidase Conjugate, Rev D
LIT420
Instruction Manual, Blotting Grade Affinity Purified Goat Anti-Mouse IgG (H + L) Alkaline Phosphatase Conjugate, Rev D
LIT421
Instruction Manual, Blotting Grade Affinity Purified Goat Anti-Human IgG (H + L) Alkaline Phosphatase Conjugate
LIT440
Instruction Manual, Blotting Grade Affinity Purified Goat Anti-Human IgG ((H + L) Horseradish Peroxidase Conjugate
2032
Western Blotting Detection Reagents Brochure, Rev F