Blotting-grade HRP conjugates (horseradish peroxidase) and AP conjugates (alkaline phosphatase) produce specific results, eliminating false positives in western blotting immunoassays
- Double affinity-purified blotting-grade antibodies are isolated by affinity chromatography and further purified by cross-adsorption against an unrelated species to eliminate nonspecific immunoglobulins
- The high titer of the blotting-grade antibody conjugates increase assay sensitivity. Greater working dilutions (1:3,000) decrease background and increase the signal-to-noise ratio of the conjugated enzyme assay
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