Evolutionary Relationships

Explore evolutionary relationships at the molecular level using DNA or protein. Students investigate the difference of DNA sequences or proteins of closely related species.

	Comparative Proteomics Kit I	DNA Barcoding Kits
Overview	In this proteomics based lab, students analyze the differences of the actin and myosin muscle proteins to build an understanding that even closely related species exhibit considerable variation.	Investigate the nuances of the cytochrome c oxidase I (COI) gene or the internal transcribed spacer (ITS) of your choice of fish, mammals, insects, or fungi. Investigate fraud happening at local marketplaces or explore species variations in local habitats.
Ideal For	Teachers who want to explore evolutionary relationships by examining fish muscle proteins and want to include protein gel electrophoresis (SDS-PAGE) in their classrooms	Teachers who want to introduce students to PCR, gel electrophoresis, DNA sequencing, bioinformatics, and potentially citizen science projects
AP Alignment	Map of AP-Aligned Bio-Rad Kits with Essential Knowledge Map of AP-Aligned Bio-Rad Kits with Investigative Laboratories Map of AP-Aligned Bio-Rad Kits with Learning Objectives Inquiry Based Learning Poster
Pedagogical Support	20 Questions to Master Inquiry - Protein Profiler Kit	Fish DNA Barcoding Kit Planning Guide, Biotechnology Explorer
Class Sessions	Three 45 minute classes	Varies
Inquiry Type	Open-ended	Open-ended
Required Equipment	Adjustable Micropipets Power Supplies Water Bath or Dry Bath Gel Staining Trays Foam Floating Racks If Using Polyacrylamide Gel Electrophoresis: Vertical gel Electrophoresis Chambers (required) If Using Agarose Gel Electrophoresis: Horizontal Gel Electrophoresis Chambers (required)	Horizontal Gel Electrophoresis Chambers Adjustable Micropipets Power Supplies Thermal Cycler Microcentrifuge Gel Staining Trays Foam Floating Racks

Teachers who bought these items, also bought:

PV92 PCR Informatics Kit

In this activity, students use real-world forensic techniques to extract DNA from their hair follicles or cheek cells, and then use PCR amplification and electrophoresis to fingerprint their own DNA.
Forensic DNA Fingerprinting Kit

The Forensic DNA Fingerprinting kit allows your students to use real DNA as evidence, as they play the role of crime scene investigator to figure out for themselves "Who done it?".
Got Protein? Kit

Based on the Bio-Rad Quick Start Bradford protein assay, this kit is designed to introduce students to proteomics and provides the tools for them to develop their own protein-based experiments.