Based on the Bio-Rad Quick Start™ Bradford protein assay, the Got Protein™ Kit is designed to introduce students to proteomics and provides the tools for them to develop their own protein-based experiments. In this laboratory activity, students use absorbance values from a set of protein samples with known concentrations to create a standard curve on linear graph paper. Protein concentrations of their test samples, such as milk, egg, sports drink, or saliva, can then be extrapolated by hand or plotted using a graphing utility such as Microsoft Excel software.
Features and Benefits
- Inquiry-based activity
- Integrates biology, chemistry, and physics in relation to protein structure and function
With this kit, students are able to:
- Measure protein concentrations
- Apply Beer's law
- Learn to use a spectrophotometer
- Learn to use a micropipet
How It Works
The Bradford method is based on the color development formed when the dye Coomassie Blue G–250 binds to protein. There is a correlation between the amount of blue color and the amount of protein in the sample: the more protein, the more intense the blue color. The simplicity of the assay allows the results to be measured qualitatively by eye or quantitatively with a spectrophotometer.
- One 45 min laboratory activity
- Provides sufficient materials for eight student workstations, up to four students per workstation.
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