Got Protein? Kit

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Overview

Based on the Bio-Rad Quick Start Bradford protein assay, this kit is designed to introduce students to proteomics and provides the tools for them to develop their own protein-based experiments.
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Description

Description

Based on the Bio-Rad Quick Start™ Bradford protein assay, the Got Protein™ Kit is designed to introduce students to proteomics and provides the tools for them to develop their own protein-based experiments. In this laboratory activity, students use absorbance values from a set of protein samples with known concentrations to create a standard curve on linear graph paper. Protein concentrations of their test samples, such as milk, egg, sports drink, or saliva, can then be extrapolated by hand or plotted using a graphing utility such as Microsoft Excel software.

Features and Benefits

  • Inquiry-based activity
  • Integrates biology, chemistry, and physics in relation to protein structure and function

With this kit, students are able to:

  • Measure protein concentrations
  • Apply Beer's law
  • Learn to use a spectrophotometer
  • Learn to use a micropipet

How It Works

The Bradford method is based on the color development formed when the dye Coomassie Blue G–250 binds to protein. There is a correlation between the amount of blue color and the amount of protein in the sample: the more protein, the more intense the blue color. The simplicity of the assay allows the results to be measured qualitatively by eye or quantitatively with a spectrophotometer.

More Information

  • One 45 min laboratory activity
  • Provides sufficient materials for eight student workstations, up to four students per workstation.

Specifications

Specifications

Kit contains sufficient materials for 80 student workstations (2–4 students per workstation)
 
Quick Start™ Bradford Protein Assay Kit 4, includes 1 L of 1x dye reagent, bovine γ-globulin standard set (2 sets of 7 standards, 0.125–2.0 mg/ml, 2 ml)
1
10x PBS, 100 ml
1
1.5 ml standard disposable polystyrene cuvettes
100
Curriculum, including teacher's guide, student manual, and graphic quick guide
1

Ordering

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items

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166-2900EDU_tn.jpg
(Discontinued) Got Protein? Kit
1662900EDU
Introductory proteomics classroom study kit, includes Quick Start Bradford protein assay kit 4 (#500-0204EDU), 10x PBS, curriculum, and more, for 320 students; education use only

Refills

Refills

166-2901EDU_tn.jpg
1662901EDU
Pkg of 1, instruction manual for use with Got Protein? Kit (#166-2900EDU); education use only
500-0204EDU_tn.jpg
5000204EDU
Colorimetric protein assay kit, includes 1x dye reagent (1 L), bovine γ-globulin standard set (2 sets of 7 standards, 0.125–2.0 mg/ml, 2 ml); education use only

Documents

Documents

Number Description Options
5516
Got Protein? Kit, Biotechnology Explorer, Quick Guide, Rev A
10006836
Got Protein? Kit, Biotechnology Explorer, Instruction Manual, Rev A