Fish DNA Barcoding Kit

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Fish DNA Barcoding Kit

Overview

DNA Barcode Generator

The DNA barcode generator allows you to create a barcode in color — each line of the barcode represents a particular base pair: green = adenine, blue = cytosine, black = guanine, red = thymine.

When assembled side by side, you will create a representative color barcode for the sequence of your sample!

Just follow the instructions to generate your barcode.

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Fish DNA Barcoding Kit Flowchart
Fish DNA Barcoding Kit Flowchart
Polymerase Chain Reaction
Lab Preparation Checklist
Kit Contents (sufficient materials for 8 student workstations with 2–4 students per workstation)
Resuspension solution, 5 ml 1
Lysis solution, 5 ml 1
Neutralization solution, 5 ml 1
Wash buffer, 10 ml 1
Matrix, 5 ml 1
Spin filters 20
PCR tubes, 0.2 ml 50
Microcentrifuge tubes, 2 ml 250
PCR master mix (Taq DNA polymerase, dNTPs, buffer), 1.2 ml 1
Fish primer mix (40x), 50 µl 1
pCOI control, 25 µl 1
UView™ 6x loading dye, 200 µl 1
PCR molecular weight ruler, 200 µl 1
Sterile water, 2.5 ml 1
Curriculum (includes teacher guide, student manual, and graphic quick guide) 1
Required Accessories Not Included in Kit
Fish tissue samples 8–16
Digital dry bath/water bath 1
Model 16K centrifuge 1–2
Horizontal gel electrophoresis chambers 4–8
Power supplies 2–4
Thermal cycler 1
2–20 µl adjustable-volume micropipet 8
20–200 µl adjustable-volume micropipet 8
100–1,000 µl adjustable-volume micropipet 8
100–1,000 µl pipet tips 1 bag
2–20 µl aerosol barrier pipet tips 1 bag
20–200 µl aerosol barrier pipet tips 1 bag
UV transilluminator or imaging system 1
Ethanol 10 ml
Distilled water 5 ml
Razor blades or plastic knives 16
Electrophoresis buffer, 50x TAE, 100 ml 1
Agarose powder, 5 g 1
Gloves  
Markin pens 8
Recommended (Optional) Accessories
Precast agarose gels 8
Pipet controller 1
Vortexer 1
Digital camera for imaging gels 1

The Fish DNA Barcoding Kit is an advanced kit that incorporates PCR and DNA sequencing techniques for teaching students how to genetically identify fish species.


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  • Description
  • Specifications
  • Ordering
  • Download
  • Accessories
  • Refills
  • Documents

Description

The Fish DNA Barcoding Kit is an advanced PCR kit that allows students to determine the species of a fish sample based on its DNA sequence of the cytochrome c oxidase I gene.

Key Features and Benefits

With this kit, students are able to:

  • Perform DNA extraction, PCR amplification, and gel electrophoresis
  • Use sequencing and bioinformatics to determine fish species
  • Contribute to the global initiatives to barcode life

Background Information

Species are traditionally identified by taxonomists who need to have in-depth knowledge of certain species characteristics and environments in order to categorize them. The power of DNA research now allows us to add a level of identification based on the sequencing of a conserved gene. The subtle changes in sequences result in species-specific identification or genetic barcodes. This information is critical in helping to assess biodiversity and conservation efforts, as well as commercially, where food marketplaces can be regulated.

More Information

  • The Fish DNA Barcoding Kit (1665100EDU) provides sufficient materials for processing up to 16 fish and control samples (8 student workstations, 2 samples per workstation, 2–4 students per workstation).
  • DNA samples may be sequenced using any commercial DNA sequencing service. These services are available in most regions and at local universities. Refer to the instruction manual for sample processing requirements (sample cleanup, primers, etc.). Also, please contact the service provider you wish to use for their specific requirements and services.
  • The DNA Barcoding Sequencing Module (1665115EDU) will be discontinued effective July 1, 2021. You may still order the module until June 30, 2021 by calling Customer Care to place your order. All purchases of the DNA Sequencing Module may be used/redeemed through June 30, 2023.

Additional Resources

Download the Fish DNA Barcoding Quiz Game, a fun activity to get your students excited about the Fish DNA Barcoding lab.

Use this fun Microplate Art Pipetting Activity to help your students practice accurate pipetting skills while creating art.

Download this Fish Flashcard activity for a fun way to introduce the Fish DNA Barcoding Laboratory to your class.


Specifications

Kit Contents This kit provides sufficient materials for processing up to 16 fish samples (8 student work stations, 2 samples per workstation, 2–4 students per workstation).
Cell resuspension solution, 5 ml 1
Cell lysis solution, 5 ml 1
Neutralization solution, 5 ml 1
Wash buffer, 10 ml 1
Matrix, 5 ml 1
Spin filters 20
PCR tubes, 0.2 ml 50
Microcentrifuge tubes, 2 ml 250
PCR master mix (Taq DNA polymerase, dNTPs, buffer), 1.2 ml 1
Fish primer mix (40x), 50 µl 1
pCOI control, 25 µl 1
UView™ 6x loading dye, 200 µl 1
PCR molecular weight ruler, 200 µl 1
Sterile water, 2.5 ml 1
Curriculum 1 (includes teacher guide, student manual, and graphic quick guide)

Ordering

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Fish DNA Barcoding Kit

1665100EDU
Advanced PCR and bioinformatics kit, includes reagents for DNA extraction, PCR amplification, plastics, curriculum, for processing up to 16 fish and control samples (8 student work stations, 2 samples per workstation, 2–4 students per workstation); education use only; sequencing module sold separately

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$311.25 
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$249.00  
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DNA Barcoding Sequencing Module

1665115EDU
Sequencing module, PCR cleanup and DNA sequencing for up to 9 fish samples; prepaid service that can be claimed with an authorized sequencing vendor, sample shipping included; 2 modules required to serve 1 full Fish DNA Barcoding Kit (#166-5100EDU); education use only

This product is being discontinued soon. Please contact customer service for more information.

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Fish DNA Barcoding Dataset

SOFT-FISH-DNA-DATASET
Sequencing data generated in an online classroom to determine the species of fish samples


Accessories

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UView™ Mini Transilluminator

1660531EDU
Compact long-wave transilluminator with auto safety shutoff; education use only

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$1,131.00 
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$904.01  
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Temperature-Controlled Water Bath

1660504EDU
120 V, 6 L water bath, ambient to 100°C, includes stainless steel tank and gable cover, over-temperature protection, non-mercury thermometer, CE approved; education use only

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$800.00 
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$560.00  
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Digital Dry Bath, 120 V

1660562EDU
Stainless steel chamber and aluminum block offer rapid and even heating of up to 24 x 1.5 ml microcentrifuge tubes, CE approved; education use only

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$670.00 
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Digital Dry Bath Heating Block, 2.0 ml

1660566EDU
Pkg of 1, aluminum heating block, heats up to 24 x 2.0 ml microcentrifuge tubes, for use with digital dry bath; education use only

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$129.00 
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Professional Adjustable-Volume 100–1,000 μl Digital Micropipet

1660508EDU
Pkg of 1, 100–1,000 μl adjustable-volume micropipet, includes digital dial micrometer, tip ejector, accommodates standard pipet tips, fully autoclavable; education use only

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$337.50 
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Xcluda™ Style E Aerosol Barrier Pipet Tips

2112021EDU
Pkg of 960, 100–1,000 µl pipet tip with aerosol barrier, racked, certified free of pyrogen, DNase, RNase; education use only

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$191.00 
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$152.80  
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TBR-41 Tips

2239351EDU
Pkg of 1,000, 100–1,000 µl standard pipet tip in enclosed rack, autoclavable, natural; education use only

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$61.00 
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Professional Adjustable-Volume 20–200 μl Digital Micropipet

1660507EDU
Pkg of 1, 20–200 μl adjustable-volume micropipet, includes digital dial micrometer, tip ejector, accommodates standard pipet tips, fully autoclavable; education use only

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$337.50 
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Xcluda™ Style D Aerosol Barrier Pipet Tips

2112016EDU
Pkg of 960, 20–200 µl pipet tip with aerosol barrier, racked, certified free of pyrogen, DNase, RNase; education use only

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$191.00 
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Professional Adjustable-Volume 2–20 μl Digital Micropipet

1660506EDU
Pkg of 1, 2–20 μl adjustable-volume micropipet, includes digital dial micrometer, tip ejector, accommodates standard pipet tips, fully autoclavable; education use only

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$337.50 
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Xcluda™ Style B Aerosol Barrier Pipet Tips

2112006EDU
Pkg of 960, 2–20 µl pipet tip with aerosol barrier, racked, certified free of pyrogen, DNase, RNase; education use only

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$209.00 
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$167.20  
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Mini-Sub® Cell GT Cell Tank and Lid

1664000EDU
Agarose gel electrophoresis cell, includes tank, lid, cables, 7 x 10 cm gel tray, two 8-well combs, does not include casing gates; education use only

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$367.85 
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$178.57  
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PowerPac Basic Power Supply

1645050EDU
Power supply for basic applications such as submerged horizontal gel electrophoresis, includes power cord

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$416.00 
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$299.00  
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Model 16K Microcentrifuge

1660602EDU
120 V, 18-tube benchtop centrifuge, 14,000 rpm (16,000 x g), includes rotor for either 1.5 or 2.0 ml tubes, safe for coldroom, CE approved; education use only

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$2,840.00 
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T100™ Thermal Cycler

1861096EDU
Thermal cycler system, includes 96-well thermal cycler, power cord, tube support ring; educational use only

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$5,211.00 
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Green Racks

1660481EDU
Pkg of 5, for organizing up to 80 x 1.5/2.0 ml microcentrifuge tubes in each rack; education use only

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$76.00 
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$53.00  
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96-Place Racks

TRC0501EDU
Pkg of 5, rack for PCR tubes and unskirted microplates, with covers, assorted colors; education use only

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$45.00 
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Refills

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DNA Extraction Reagent Pack

1665105EDU
Refill kit, includes resuspension, lysis, and neutralization solutions, wash buffer, matrix and spin filters for up to 16 extractions; education use only

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$122.50 
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Fish DNA Barcoding Reagent Refill Pack

1665110EDU
Temperature-sensitive refill kit, includes PCR master mix, primers, positive and negative controls, molecular weight ruler, and UView™ 6x loading dye; education use only

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$217.50 
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UView™ 6x Loading Dye, 200 µl

1665111EDU
200 µl, 6x dual-action nontoxic fluorescent nucleic acid stain and loading dye; education use only

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$32.19 
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$25.75  
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UView™ 6x Loading Dye, 1 ml

1665112EDU
1,000 µl, 6x dual-action nontoxic fluorescent nucleic acid stain and loading dye; education use only

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$97.85 
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$78.28  
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PCR Kleen™ Spin Purification Module

7326300EDU
Fourth module in Cloning and Sequencing Explorer Series classroom study kit, includes PCR Kleen Spin columns, test tubes, curriculum, for 12 workstations; education use only

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$105.00 
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Certified™ Molecular Biology Agarose

1613116EDU
5 g, general purpose agarose for nucleic acid electrophoresis, high gel strength, certified free of inhibitors, DNases, RNases; education use only

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$8.00 
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$6.40  
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50x Tris/Acetic Acid/EDTA (TAE)

1660742EDU
100 ml, TAE nucleic acid electrophoresis buffer solution, pH 8.0; education use only

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$18.75 
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Documents

Number Description Options
6672 Map of AP-Aligned Bio-Rad Kits with Essential Knowledge Click to download
6673 Map of AP-Aligned Bio-Rad Kits with Investigative Laboratories Click to download
6605 Map of AP-Aligned Bio-Rad Kits with Learning Objectives Click to download
6353 Fish DNA Barcoding Kit Planning Guide, Biotechnology Explorer, Rev A Click to download
6398 Biotechnology Explorer™ Fish DNA Barcoding Kit Bioinformatics Guide, Rev A Please login to download this document
10028899 Instruction Manual, Fish DNA Barcoding Kit, Biotechnology Explorer™, Rev A Please login to download this document
6410 Instruction Manual, Biotechnology Explorer™ Fish DNA Barcoding Kit Quick Guide, Rev A Click to download
6602 UView™ 6x Loading Dye and Stain Guide, Bio-Rad Explorer™ Click to download

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