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Bio-Plex Pro Human SARS-CoV-2 Neutralization Antibody Assays
Product Information SheetSee Performance Data
Bio-Plex Pro SARS-CoV-2 Multiplex Serology Assays Flier
The Bio-Plex Pro SARS-CoV-2 Serology Assays* provide COVID-19 researchers with sensitive and specific assays to monitor the antibody immune response profile against four viral antigens. There are three isotype-specific 4-plex panels that measure antibodies against SARS-CoV-2 Nucleocapsid, Receptor Binding Domain, Spike 1, and Spike 2 viral proteins. The isotype-specific antibodies detected are IgA, IgG, and IgM, providing the spectrum of humoral response over time after COVID-19 vaccine administration or natural exposure to SARS-CoV-2.
Bio-Plex Pro SARS-CoV-2 neutralization assays allow researchers to assess the efficacy of COVID-19 vaccines and compare effectiveness of naturally acquired or vaccine-induced antibody response to arising variants of concern. These highly precise and sensitive assays quantitatively measure SARS-CoV-2 neutralizing antibodies against two wild type and 11 variants of receptor binding domain and spike 1 protein subunits. Antigens are coated on magnetic beads that bind neutralizing antibodies in competition with a biotinylated detection ACE-2 receptor.
SARS-CoV-2 Wild Type and Variant S1 and RBD Antigens
- Wild type RBD
- Wild type S1
- Alpha S1
- Beta S1
- Gamma RBD
- Delta RBD
- Delta Spike Trimer
- Epsilon RBD
- Kappa RBD
- D614G S1
- N501Y RBD
- K417N RBD
- E484K RBD
In this rapidly changing SARS-CoV-2 variant landscape, the Bio-Plex Human SARS-CoV-2 Neutralization Antibody Custom Assay Developer Kit, can be used to develop neutralization assays with any variant of interest. It comes with the Bio-Plex Pro Amine Coupling Kit, a vial of Bio-Plex Pro Magnetic COOH Beads 27, and Bio-Plex Pro Serology Beads Storage Buffer.
Individual assay components are also available, offering the flexibility to assay only the IgA, IgG, IgM, or neutralization antibodies of interest. The viral-protein coated beads can be used to assay antibodies specific to SARS-CoV-2 in multiple species and only need to be paired with a species-specific biotinylated detection antibody for the serology assays. Bio-Plex Pro reagent kits can be used to further streamline assay configuration.
Individual Assay Component Ordering Information »
COVID-19 Vaccine Research and Development and Clinical Trials
The immune response to vaccination is multifaceted, with elements of the innate and adaptive immune response reacting to the target immunogen. Capturing the antibody response is one aspect that can be used to determine a vaccine's efficacy.
The Bio-Plex SARS-CoV-2 serology and neutralization assays enable you to:
- Detect antibody response to specific viral subunits and quantitatively measure neutralizing antibodies to understand each protein’s immunogenicity profile
- Monitor dosing levels and efficacy in preclinical and clinical trials
- Determine effectiveness of vaccine over time through seropositive results during post-market surveillance
IgA, IgG, and IgM Multiplex Serology and Neutralization Assay Kits
Serology Assays: SARS-CoV-2 N, RBD, S1, and S2 Coupled Beads and Neutralization Assays: SARS-CoV-2 RBD and S1 Wild Type and Variant Coupled Beads
Serology Assays: Anti-IgA, IgG, and IgM Antibodies and Neutralization Assays: Detection ACE-2 Receptor
Serology Assays: Control Sets and Neutralization Assays: Standard and Control
Publications For Multiplex Immunoassay Use in Vaccine Development
- Trivedi SU, Miao C, Sanchez JE, Caidi H, Tamin A, Haynes L, Thornburg NJ. Development and Evaluation of a Multiplexed Immunoassay for Simultaneous Detection of Serum IgG Antibodies to Six Human Coronaviruses. Sci Rep. 2019 Feb 4;9(1):1390. DOI: 10.1038/s41598-018-37747-5
- Itell HL et al. (2018) Development and application of a multiplex assay for the simultaneous measurement of antibody responses elicited by common childhood vaccines. Vaccine. 2018 Sep 5;36(37):5600-5608. DOI: 10.1016/j.vaccine.2018.07.048
- Nygård M et al. (2018) Evaluation of the Long-Term Anti-Human Papillomavirus 6 (HPV6), 11, 16, and 18 Immune Responses Generated by the Quadrivalent HPV Vaccine. Clin Vaccine Immunol. 2015 Aug;22(8):943-8. DOI: 10.1128/CVI.00133-15
SARS-CoV-2 Antibody Assays for Public Health Studies
Surveying the seroprevalence of antibodies to the SARS-CoV-2 virus is key to understanding local infection and exposure rates in specific areas or institutions. Because some people are asymptomatic and may not know they have been infected, population studies are crucial in modeling transmission in the COVID-19 pandemic.
Bio-Plex SARS-CoV-2 Antibody Assays provide flexible options to measure anti-SARS-CoV-2 IgG antibodies in a population. Samples can be assayed to measure any combination of antibodies against SARS-CoV-2 nucleocapsid, receptor-binding domain, spike 1, and spike 2 viral proteins.
Publications For Multiplex Immunoassay Use in Seroprevalence Studies
- Fenwick C et al. (2020) Changes in SARS-CoV-2 Antibody Responses Impact the Estimates of Infections in Population-Based Seroprevalence Studies. medRxiv 2020.07.14.20153536; DOI: 10.1101/2020.07.14.20153536
- Kamminga S (2018) Seroprevalence of fourteen human polyomaviruses determined in blood donors. PLoS One. 2018 Oct 23;13(10):e0206273. DOI: 10.1371/journal.pone.0206273
- Kaur H et al. (2018) Evaluation of the reproducibility of a serological test for antibodies to Chlamydia trachomatis pgp3: A potential surveillance tool for trachoma programs. J Microbiol Methods. 2018 Apr;147:56-58. DOI: 10.1016/j.mimet.2018.02.017
Bio-Plex SARS-CoV-2 Assays Ordering Options
Multiplex SARS-CoV-2 Serology and Neutralization Ab Assays Kits
Select complete, ready-to-use kits for multiplex target panels. For human samples only.
SARS-CoV-2 Assay Documents & Resources
NEW COVID-19 eBook
Sero-Surveillance: Immunoassays for Combating the COVID-19 Pandemic
Find info about kit contents, storage, workflows, detailed instructions, and important considerations with this manual.
Read this quick guide for protocols on how to prepare and run assays.
Learn more about the assay with detailed instructions, workflows, kit contents, storage, and troubleshooting tips with this instruction manual.
Find out how to prepare and run a full 96-well Bio-Plex SARS-CoV-2 Serology Assay plate with this quick guide.
Obtain product details such as assay characteristics and cross-reactivity results with this product information sheet.
Produce results with this semiquantitative protocol for use with the Bio-Plex Human IgG SARS-CoV-2 N/RBD/S1/S2 Serology Assays.
Generate results with this protocol for use with Bio-Plex Human IgA and IgM SARS-CoV-2 Serology Assays.
Bio-Plex Multiplex Systems Brochure (PDF 15.9 MB)
Learn about how how you can reduce time to results and samples while discovering more with Bio-Plex multiplex immunoassays, instruments, and software.
Quantitative Bio-Plex Pro Human IgG, IgA, and IgM SARS-CoV-2 Serology Assays Using a Standard Curve Application Note (PDF 420 KB)
Learn how the VIROTROL SARS-CoV-2 Serological Control is validated for use as a reference material to quantitate levels of SARS-CoV-2–specific IgG, IgA, and IgM antibodies using the Bio-Plex Pro Human IgG, IgA, and IgM SARS-CoV-2 Serology Assays in this application note.
Bio-Plex Analyte Guide (PDF 2.2 MB)
Explore the different multiplex immunoassays and panels available on the Bio-Plex platform. Evaluate assay performance characteristics and sample data to choose the panel that fits your application.
Bio-Plex Amine Coupling Kit Instruction Manual (PDF 259 KB)
Find info about kit contents, storage, workflows, detailed instructions, and important considerations with this manual. The amine coupling kit is used to develop new SARS-CoV-2 Variant assay with Bio-Plex Pro Human SARS-CoV-2 Neutralization Antibody Custom Assay Developer Kit (17007632).
Development of New SARS-CoV-2 Variant Neutralization Antibody Assays Using the Bio-Plex Pro Human SARS-CoV-2 Neutralization Antibody Custom Assay Developer Kit Application Note (PDF 12 KB)
Learn how this new Bio-Plex Developer Kit can be used to rapidly develop assays for determining the percentage inhibition of neutralization antibodies against SARS-CoV-2 variants.
Calibration Factors Using WHO International Standard (NIBSC 20/136) for Anti–SARS-CoV-2 Immunoglobulins Flier (PDF 135 KB)
Reference WHO conversion factors to compare SARS-CoV-2 serology immunoassay results among different manufacturers and platforms.
In this webinar, we discuss early research and nascent hypotheses regarding the pathophysiology of SARS-CoV-2 induced COVID-19 disease by evaluating cytokine and chemokine profiles, the role of chronic inflammation in comorbidities, and the arc of immune resolution of historical virulent pathogens, such as SARS and MERS.
Get the most data out of your sample, without spending time on individual ELISAs. Learn how multiplex assays are being used on the front lines to understand patient immune response during infection, and discuss the most commonly asked questions around targets, panels, and multi-plate data analysis.
Tips for overcoming developmental hurdles, technologies that can shorten timelines, and novel tools for manufacturing and quality control.
In this webinar, we will be discussing the immune response to SARS‑CoV-2, both in the context of natural exposure to the virus as well as induced immunity from vaccination. We will also investigate the relationship between symptom severity and the longevity of acquired immunity.
Bio-Rad provides multiple solutions to study the host response to coronavirus infection including assays to profile cytokine levels, immune and inflammation markers, and flow cytometry solutions to monitor cellular immune response.
Bio-Plex Readers and Tools include the Bio-Plex 200 and Bio-Plex 3D Systems, Bio-Plex Pro Wash Station, and maintenance, calibration, verification, and validation kits.