Bio-Plex Multiplex Immunoassay System
Bio-Plex Assays, Instruments & Software
Quantify over 500 different protein and peptide targets simultaneously in a single 12.5 µl sample using the Bio-Plex Multiplex System, powered by Luminex xMAP technology. The Bio-Plex System offers high-performance readers, industry-leading software, and sensitive assays in ready-to-use or custom configurations, enabling you to obtain high-quality data from limited samples.
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Bio-Plex Assay Finder
Find Bio-Plex Immunoassays to quantify over 450 biological analytes. Select your targets, filter by species: Human, Mouse, Rat, Canine, or Primate.
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Bio-Plex Assays, Kits & Reagents
Bio-Plex Multiplex immunoassays use Luminex magnetic beads for the quantification of over 450 biologically relevant targets: assays for inflammation, disease, cancer, cell signaling and growth, apoptosis, toxicity, and more. Choose assays in premade and custom configurations, or develop your own assays for new targets.
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Bio-Plex Instruments
Bio-Plex Readers and Tools include the Bio-Plex 200 and Bio-Plex 3D Systems, Bio-Plex Pro Wash Station, and maintenance, calibration, verification, and validation kits.
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Bio-Plex Software
Bio-Plex Manager and Bio-Plex Data Pro Software make it simple to run instruments using xMAP technology and analyze multiplex data.
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Bio-Plex Standard Lot Data
Download data for released standard lots for import into Bio-Plex Manager Software.
Free 96-Well Plate Template Post-It Pad
Simplifies the Bio-Plex Multiplex Assay process from start to finish.
Comparison of Bio-Plex Multiplexing vs. ELISA
* Samples run in duplicate.
** Calculated as 2.2 hours required per plate.
*** Assumes 50 μl of sample used per well.
Resources
Bio-Plex Multiplex Immunoassay Manuals, Guides & Protocols Library
Find protocols for all the Bio-Plex assay groups, Multiplex Immunoassays Success Guides for sample prep and hardware & software use, and a calculation worksheet with a plate layout template.
Bio-Plex System Applications & Uses
Watch short videos to learn how the Bio-Plex system works, how it is used in cardiovascular disease and leukemia research, biomarker identification, and citokinin expression in cancer.
Bio-Plex™ Pro Multiplex Immunoassays Analyte Guide
(PDF 5 MB)
Bio-Plex Pro Multiplex Immunoassays Brochure
(PDF 4 MB)
Bio-Plex Assay and Sample Prep Success Guide
(PDF 390 KB)
Bio-Plex Hardware and Software Success Guide
(PDF 319 KB)
Bio-Plex Applications
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Bench to Bedside - Learn how Bio-Plex assays and instruments are being used in cardiovascular disease and leukemia research.
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Understanding Melanoma Biomarkers - Learn how the Bio-Plex system can help to identify early markers of melanoma in serum and blood samples from at-risk patients.
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Understanding Cancer - Learn how Bio-Plex assays and instruments are being used to monitor cytokine expression in cancer and in response to cancer vaccines.
Bio-Plex Quick Tip Videos
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Bio-Plex Quick Tips — Planning for Assay Success Part 1
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Bio-Plex Quick Tips — Planning for Assay Success Part 2
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Bio-Plex Quick Tips — Planning for Assay Success Part 3
Application Notes
Learn about drug toxicity monitoring in kidney proximal tubule chips
(PDF 681 KB)
Normal Physiological Levels of Human Cytokines Using Bio-Plex Pro Cytokine Assays
(PDF 140 KB)
Development and Validation of Bio-Plex Pro Human Chemokine Assays
(PDF 284 KB)
Multiplex Analysis of Cell Death Markers Using Bio-Plex Pro RBM Apoptosis Assays
(PDF 569 KB)
Quantitative Bio-Plex Pro Human IgG, IgA, and IgM SARS-CoV-2 Serology Assays Using a Standard Curve
(PDF 420 KB)
Bio-Plex Custom Assay Development Reagents
(PDF 67 KB)-
Bio-Plex Pro Human IgA, IgG, and IgM SARS-CoV-2 Serology Assay Performance in Freeze-Thawed and Refrigerated Serum, Plasma, and Saliva Samples
(PDF 267 KB) 
Development of New SARS-CoV-2 Variant Neutralization Antibody Assays Using Bio-Plex Pro Human SARS-CoV-2 Neutralization Antibody Custom Assay Developer Kit
(PDF 386 KB)-
Correlation of SARS-CoV-2 Live Virus Neutralization Assay to Bio-Plex™ Pro Human SARS-CoV-2 Variant Neutralization Antibody Assays
(PDF 273 KB)
Multiplex Immunodetection Webinar Series
Join Bio-Rad multiplex immunodetection experts for a webinar series that explores the many factors that go into the design and execution of successful xMAP multiplex immunoassays. Not only will we cover the technology and general how to’s, we’ll also delve into the why’s to give you a deeper understanding and get you on the right path for success. By the end of this series, you’ll be a multiplexing expert.
Applications & Technologies
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Multiplex Immunoassays
Details on the experimental design, data collection, analysis, and validation of multiplex protein assays using the Bio-Plex Suspension Array System.
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Biomarker Analysis
Learn how biomarkers such as hormones, cytokines, chemokines, growth factors, and phosphoproteins are used to investigate physiological or pharmacological mechanisms.
