Bio-Plex Multiplex SARS-CoV-2 Neutralization Antibody Assay Kits and Reagents

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Bio-Plex SARS-CoV-2 Kit

Overview

Provides highly precise and specific assays to measure SARS-CoV-2 neutralizing antibodies against two wild-type and 11 SARS-CoV-2 variants.

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Description

Description

Bio-Plex SARS-CoV-2 Neutralization Antibody Assays Webinar

Bio-Plex Pro Human SARS-CoV-2 Neutralization Antibody Assays* allow researchers to assess the efficacy of COVID-19 vaccines or compare the effectiveness of naturally acquired or vaccine-induced antibody response to arising variants of concern. The highly precise and specific assays are offered as an 11-Plex 96-well plate complete kit that includes Wild-type S1, Wild-type RBD, Alpha Spike 1, Beta Spike 1, Gamma RBD, Epsilon RBD, Kappa RBD, D614G Spike 1, E484K RBD, K417N RBD, and N501 RBD. The wild-type S1 and RBD are also available as a 2-plex 96-well plate kit. For flexibility, the variants are available as coupled beads. In addition, the Delta RBD and Delta Spike Trimer are available as 2-plex coupled beads. SARS-CoV-2 antigens are coated on magnetic beads that bind neutralizing antibodies in competition with a biotinylated detection ACE2 receptor.

*Bio-Plex Pro SARS-CoV-2 Neutralization Antibody Assays are for research use only.

Features and Benefits

  • Comprehensive panel coverage — detects neutralizing antibody response to two wild-type and 11 SARS-CoV-2 variants
  • Fast protocol — results in less than 3 hours
  • High precision and specificity — for greater confidence in measuring SARS-CoV-2 specific antibodies in serum and plasma samples
  • Reproducibility — attain consistent results in your experiments with a robust magnetic bead-based workflow

Ordering Options for Bio-Plex Neutralization Antibody Assays

  • Select complete kits for multiplex target panels
  • Create multiplex assays with singleplex SARS-CoV-2 neutralization variant antigen coupled beads
  • Develop your own variant assay with Bio-Plex Pro Human SARS-CoV-2 Neutralization Antibody Custom Assay Developer Kit

How to Order Individual Bio-Plex SARS-CoV-2 Neutralization Antibody Assay Components

Human samples require four components for a complete assay:

  • Bio-Plex Pro SARS-CoV-2 wild-type or variant antigen coupled beads
  • Bio-Plex Pro SARS-CoV-2 Neutralization Antibody Reagent Kit (12017037)
  • Bio-Plex Pro SARS-CoV-2 Neutralization Antibody Standard (12016945)
  • Bio-Plex Pro Biotinylated Detection ACE2 Receptor (12016944)

For samples from other species, researchers must provide their own positive control, and standard. The appropriate species-specific reagent kit should also be used.

  • Bio-Plex Pro Reagent Kit V with Flat Bottom Plate, 1 x 96-well (12002798)

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Bio-Plex SARS-CoV-2 Variant
12016897

1 x 96-well, includes SARS-CoV-2 wild-type and variant coupled magnetic capture beads, biotinylated detection ACE2 receptor, standard, ready-to-use positive control, assay buffer, wash buffer, serology sample diluent, streptavidin-phycoerythrin (SA-PE), 96-well flat bottom plate, and sealing tape. For the detection of human neutralization antibodies against SARS-CoV-2 RBD, S1, Alpha S1, Beta S1, Gamma RBD, D614G S1, E484K RBD, Epsilon RBD, K417N RBD, Kappa RBD, N501Y RBD antigens.

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Bio-Plex SARS-CoV-2 NAb thumbnail
12016848

1 x 96-well, includes SARS-CoV-2 wild-type Receptor Binding Domain (RBD) and Spike 1 (S1) coupled magnetic beads, biotinylated detection ACE2 receptor, standard, ready-to-use positive control, assay buffer, wash buffer, serology sample diluent, streptavidin-phycoerythrin (SA-PE), flat bottom plate, and sealing tape. For the detection of human neutralization antibodies against SARS-CoV-2 wild-type RBD and Spike 1 antigens.

Kits & Reagents

Kits & Reagents

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Bio-Plex Pro Human SARS-CoV-2 Neutralization Antibody Reagent Kit Thumbnail
12017037

1 x 96 well, assay buffer, wash buffer, serology sample diluent, streptavidin-PE, flat-bottom plate, and sealing tape. To be used with Bio-Plex Pro SARS-COV-2 Antigen Coupled beads, Bio-Plex Pro Neutralization Antibody Standard, and Bio-Plex Pro Biotinylated Detection ACE2 Receptor.

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Bio-Plex Biotinylated ACE2 Receptor Assay Thumbnail
12016944

1 x 96-well, biotinylated detection ACE2 receptor. The SARS-CoV-2 spike protein binds to the ACE2 receptor during viral entry. Requires Bio-Plex Pro Neutralization Reagent Kit, Bio-Plex Pro Neutralization Antibody Standard, and Bio-Plex Pro SARS-CoV-2 Antigen Coupled Beads to run a complete singleplex or multiplex assay.

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Bio-Plex Pro Human SARS-CoV-2 Neutralization Antibody Standard Thumbnail
12016945

Pkg of 1 vial, Neutralization Antibody Standard binds to SARS-CoV-2 viral antigens, enough to generate a standard curve in duplicate for 1 x 96 well plate; for use with Bio-Plex Pro SARS-CoV-2 Antigen Coupled Beads, Bio-Plex Pro Biotinylated Detection ACE2 Receptor, Bio-Plex Pro SARS-CoV-2 Neutralization Antibody Reagent Kit.

Assay Developer Kit

Assay Developer Kit

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Bio-Plex SAR-CoV-2 Antibody Kit Thumbnail
17007632

SARS-CoV-2 Neutralization Antibody Custom Assay Developer Kit. Includes Bio-Plex Pro Human SARS-COV-2 Neutralization Antibody 2-Plex Panel Kit, Bio-Plex Amine Coupling Kit for coupling proteins to magnetic beads (bead wash and activation buffers, storage and staining buffers, tubes), Bio-Plex Pro Magnetic COOH Beads 27, and Bio-Plex Pro Serology Beads Storage Buffer.

Coupled Beads

Coupled Beads

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Bio-Plex SARS-CoV-2 Alpha S1 Coupled Beads Thumbnail
12016868

Pkg of 1, SARS-CoV-2 Alpha S1 Viral Antigen Coupled Magnetic Beads for capturing neutralization antibodies that bind to Alpha Spike 1 viral antigen, sufficient for 96 reactions. Requires Bio-Plex Pro Human SARS-CoV-2 Neutralization Antibody 2-Plex Panel for a complete assay or the use with biotinylated detection ACE2 receptor, Bio-Plex Pro Neutralization Reagent Kit, and standard to run a singleplex assay.

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Bio-Plex SARS-CoV-2 Beta S1 Coupled Beads Thumbnail
12016849

Pkg of 1, SARS-CoV-2 Beta S1 Viral Antigen Coupled Magnetic Beads for capturing neutralization antibodies that bind to Beta Spike 1 viral antigen, sufficient for 96 reactions. Requires Bio-Plex Pro Human SARS-CoV-2 Neutralization Antibody 2-Plex Panel for a complete assay or the use with biotinylated detection ACE2 receptor, Bio-Plex Pro Human Neutralization Reagent Kit, and standard to run a singleplex assay.

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Bio-Plex Pro SARS-CoV-2 Gamma RBD Coupled Beads Thumbnail
12016898

Pkg of 1, SARS-CoV-2 Gamma RBD Viral Antigen Coupled Magnetic Beads for capturing neutralization antibodies that bind to Gamma RBD viral antigen, sufficient for 96 reactions. Requires Bio-Plex Pro Human SARS-CoV-2 Neutralization Antibody 2-Plex Panel for a complete assay or the use with biotinylated detection ACE2 receptor, Bio-Plex Pro Neutralization Reagent Kit, and standard to run a singleplex assay.

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Bio-Plex Pro SARS-CoV-2 Delta RBD and Spike Trimer 2-Plex Coupled Beads
12017225

Pkg of 1, SARS-CoV-2 Delta RBD and Spike Trimer 2-Plex Viral Antigen Coupled Magnetic Beads for qualitatively capturing neutralization antibodies that bind to Delta RBD and Spike Trimer viral antigens, sufficient for 96 reactions. A ready-to-use positive control is included with the Delta 2-plex coupled beads. Requires Bio-Plex Pro Human SARS-CoV-2 Neutralization Antibody 2-Plex Panel for a complete assay or the use with biotinylated detection ACE2 receptor, and Bio-Plex Pro Neutralization Reagent Kit to run a 2-plex assay.

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Bio-Plex Pro SARS-CoV-2 Epsilon RBD Coupled Beads
12016875

Pkg of 1, SARS-CoV-2 Epsilon RBD Viral Antigen Coupled Magnetic Beads for capturing neutralization antibodies that bind to Epsilon RBD viral antigen, sufficient for 96 reactions. Requires Bio-Plex Pro Human SARS-CoV-2 Neutralization Antibody 2-Plex Panel for a complete assay or the use with biotinylated detection ACE2 receptor, Bio-Plex Pro Neutralization Reagent Kit, and standard to run a singleplex assay.

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Bio-Plex Pro SARS-CoV-2 Kappa RBD Coupled Beads Thumbnail
12016850

Pkg of 1, SARS-CoV-2 Kappa RBD Viral Antigen Coupled Magnetic Beads for capturing neutralization antibodies that bind to Kappa RBD viral antigen, sufficient for 96 reactions. Requires Bio-Plex Pro Human SARS-CoV-2 Neutralization Antibody 2-Plex Panel for a complete assay or the use with biotinylated detection ACE2 receptor, Bio-Plex Pro Neutralization Reagent Kit, and standard to run a singleplex assay.

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12014772-th.jpg
12015406

Pkg of 1, SARS-CoV-2 RBD viral protein coupled magnetic beads, for capturing anti-SARS-CoV-2 antibodies specific for RBD from multispecies serum or plasma, sufficient for 96 reactions; Requires isotype-specific detection antibody, reagent kit, and isotype-specific control set.

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12014771-th.jpg
12014771

Pkg of 1, SARS-CoV-2 S1 viral protein coupled magnetic beads, for capturing anti-SARS-CoV-2 antibodies specific for Spike 1 from multispecies serum or plasma, sufficient for 96 reactions; Requires isotype-specific detection antibody, reagent kit, and isotype-specific control set.

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Bio-Plex Pro SARS-CoV-2 D614G S1 Coupled Beads Thumbnail
12016838

Pkg of 1, SARS-CoV-2 D614G S1 Viral Antigen Coupled Magnetic Beads for capturing neutralization antibodies that bind to D614G Spike 1 viral antigen, sufficient for 96 reactions. Requires Bio-Plex Pro Human SARS-CoV-2 Neutralization Antibody 2-Plex Panel for a complete assay or the use with biotinylated detection ACE2 receptor, Bio-Plex Pro Neutralization Reagent Kit, and standard to run a singleplex assay.

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Bio-Plex Pro SARS-CoV-2 E484K RBD Coupled Beads Thumbnail
12016943

Pkg of 1, SARS-CoV-2 E484K RBD Viral Antigen Coupled Magnetic Beads for capturing neutralization antibodies that bind to E484K RBD viral antigen, sufficient for 96 reactions. Requires Bio-Plex Pro Human SARS-CoV-2 Neutralization Antibody 2-Plex Panel for a complete assay or the use with biotinylated detection ACE2 receptor, Bio-Plex Pro Neutralization Reagent Kit, and standard to run a singleplex assay.

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Bio-Plex Pro SARS-CoV-2 K417N RBD Coupled Beads Thumbnail
12016942

Pkg of 1, SARS-CoV-2 K417N RBD Viral Antigen Coupled Magnetic Beads for capturing neutralization antibodies that bind to K417N RBD viral antigen, sufficient for 96 reactions. Requires Bio-Plex Pro Human SARS-CoV-2 Neutralization Antibody 2-Plex Panel for a complete assay or the use with biotinylated detection ACE2 receptor, Bio-Plex Pro Human Neutralization Reagent Kit, and standard to run a singleplex assay.

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Bio-Plex Pro SARS-CoV-2 N501Y RBD Coupled Beads Thumbnail
12016869

Pkg of 1, SARS-CoV-2 N501Y RBD Viral Antigen Coupled Magnetic Beads for capturing neutralization antibodies that bind to N501Y RBD viral antigen, sufficient for 96 reactions. Requires Bio-Plex Pro Human SARS-CoV-2 Neutralization Antibody 2-Plex Panel for a complete assay or the use with biotinylated detection ACE2 receptor, Bio-Plex Pro Neutralization Reagent Kit, and standard to run a singleplex assay.

Documents

Documents

Number Description Options
10000147006
Instruction Manual, Bio-Plex Pro Human SARS-CoV-2 Neutralization Antibody Assays
10000147009
Bio-Plex Pro Human SARS-CoV-2 Neutralization Antibody Assays Quick Guide
3263
Bio-Plex™ Pro Human SARS-CoV-2 Neutralization Antibody Assays Product Information Sheet
10000148774
Bio-Plex Amine Coupling Kit Instruction Manual
3286
Development of New SARS-CoV-2 Variant Neutralization Antibody Assays Using the Bio-Plex Pro Human SARS-CoV-2 Neutralization Antibody Custom Assay Developer Kit Application Note
3345
Correlation of SARS-CoV-2 Live Virus Neutralization Assay to Bio-Plex™ Pro Human SARS-CoV-2 Variant Neutralization Antibody Assays