Bio-Plex Multiplex SARS-CoV-2 Neutralization Antibody Assay Kits and Reagents

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Bio-Plex SARS-CoV-2 Kit

Overview

Provides highly precise and specific assays to measure SARS-CoV-2 neutralizing antibodies against two wild-type and 11 SARS-CoV-2 variants.

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Description

Description

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Bio-Plex SARS-CoV-2 Neutralization Antibody Assays Webinar

Bio-Plex Pro Human SARS-CoV-2 Neutralization Antibody Assays* allow researchers to assess efficacy of COVID-19 vaccines or compare the effectiveness of naturally acquired or vaccine-induced antibody response to arising variants of concern. The highly precise and specific assays measure SARS-CoV-2 neutralizing antibodies against two wild-type SARS-CoV-2 Receptor Binding Domain and Spike 1 and 11 variants (Alpha Spike 1, Beta Spike 1, Delta RBD, Delta Spike Trimer, Gamma RBD, Epsilon RBD, Kappa RBD, D614G Spike 1, E484K RBD, K417N RBD, and N501 RBD). SARS-CoV-2 antigens are coated on magnetic beads that bind neutralizing antibodies in competition with a biotinylated detection ACE2 receptor.

*Bio-Plex Pro SARS-CoV-2 Neutralization Antibody Assays are for research use only.

Features and Benefits

  • Comprehensive panel coverage — detects neutralizing antibody response to two wild-type and 11 SARS-CoV-2 variants
  • Fast protocol — results in less than 3 hours
  • High precision and specificity — for greater confidence in measuring SARS-CoV-2 specific antibodies in serum and plasma samples
  • Reproducibility — attain consistent results in your experiments with a robust magnetic bead-based workflow

Ordering Options for Bio-Plex Neutralization Antibody Assays

  • Select complete kits for multiplex target panels
  • Create multiplex assays with singleplex SARS-CoV-2 neutralization variant antigen coupled beads
  • Develop your own variant assay with Bio-Plex Pro Human SARS-CoV-2 Neutralization Antibody Custom Assay Developer Kit

How to Order Individual Bio-Plex SARS-CoV-2 Neutralization Antibody Assay Components

Human samples require four components for a complete assay:

  • Bio-Plex Pro SARS-CoV-2 wild-type or variant antigen coupled beads
  • Bio-Plex Pro SARS-CoV-2 Neutralization Antibody Reagent Kit (12017037)
  • Bio-Plex Pro SARS-CoV-2 Neutralization Antibody Standard (12016945)
  • Bio-Plex Pro Biotinylated Detection ACE2 Receptor (12016944)

For samples from other species, researchers must provide their own positive control, and standard. The appropriate species-specific reagent kit should also be used.

  • Bio-Plex Pro Reagent Kit V with Flat Bottom Plate, 1 x 96-well (12002798)

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Bio-Plex SARS-CoV-2 Variant
12016897

1 x 96-well, includes SARS-CoV-2 wild-type and variant coupled magnetic capture beads, biotinylated detection ACE2 receptor, standard, ready-to-use positive control, assay buffer, wash buffer, serology sample diluent, streptavidin-phycoerythrin (SA-PE), 96-well flat bottom plate, and sealing tape. For the detection of human neutralization antibodies against SARS-CoV-2 RBD, S1, Alpha S1, Beta S1, Gamma RBD, D614G S1, E484K RBD, Epsilon RBD, K417N RBD, Kappa RBD, N501Y RBD antigens.

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Bio-Plex SARS-CoV-2 NAb thumbnail
12016848

1 x 96-well, includes SARS-CoV-2 wild-type Receptor Binding Domain (RBD) and Spike 1 (S1) coupled magnetic beads, biotinylated detection ACE2 receptor, standard, ready-to-use positive control, assay buffer, wash buffer, serology sample diluent, streptavidin-phycoerythrin (SA-PE), flat bottom plate, and sealing tape. For the detection of human neutralization antibodies against SARS-CoV-2 wild-type RBD and Spike 1 antigens.

Kits & Reagents

Kits & Reagents

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Bio-Plex Pro Human SARS-CoV-2 Neutralization Antibody Reagent Kit Thumbnail
12017037

1 x 96 well, assay buffer, wash buffer, serology sample diluent, streptavidin-PE, flat-bottom plate, and sealing tape. To be used with Bio-Plex Pro SARS-COV-2 Antigen Coupled beads, Bio-Plex Pro Neutralization Antibody Standard, and Bio-Plex Pro Biotinylated Detection ACE2 Receptor.

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Bio-Plex Biotinylated ACE2 Receptor Assay Thumbnail
12016944

1 x 96-well, biotinylated detection ACE2 receptor. The SARS-CoV-2 spike protein binds to the ACE2 receptor during viral entry. Requires Bio-Plex Pro Neutralization Reagent Kit, Bio-Plex Pro Neutralization Antibody Standard, and Bio-Plex Pro SARS-CoV-2 Antigen Coupled Beads to run a complete singleplex or multiplex assay.

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Bio-Plex Pro Human SARS-CoV-2 Neutralization Antibody Standard Thumbnail
12016945

Pkg of 1 vial, Neutralization Antibody Standard binds to SARS-CoV-2 viral antigens, enough to generate a standard curve in duplicate for 1 x 96 well plate; for use with Bio-Plex Pro SARS-CoV-2 Antigen Coupled Beads, Bio-Plex Pro Biotinylated Detection ACE2 Receptor, Bio-Plex Pro SARS-CoV-2 Neutralization Antibody Reagent Kit.

Assay Developer Kit

Assay Developer Kit

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Bio-Plex SAR-CoV-2 Antibody Kit Thumbnail
17007632

SARS-CoV-2 Neutralization Antibody Custom Assay Developer Kit. Includes Bio-Plex Pro Human SARS-COV-2 Neutralization Antibody 2-Plex Panel Kit, Bio-Plex Amine Coupling Kit for coupling proteins to magnetic beads (bead wash and activation buffers, storage and staining buffers, tubes), Bio-Plex Pro Magnetic COOH Beads 27, and Bio-Plex Pro Serology Beads Storage Buffer.

Coupled Beads

Coupled Beads

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Bio-Plex SARS-CoV-2 Alpha S1 Coupled Beads Thumbnail
12016868

Pkg of 1, SARS-CoV-2 Alpha S1 Viral Antigen Coupled Magnetic Beads for capturing neutralization antibodies that bind to Alpha Spike 1 viral antigen, sufficient for 96 reactions. Requires Bio-Plex Pro Human SARS-CoV-2 Neutralization Antibody 2-Plex Panel for a complete assay or the use with biotinylated detection ACE2 receptor, Bio-Plex Pro Neutralization Reagent Kit, and standard to run a singleplex assay.

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Bio-Plex SARS-CoV-2 Beta S1 Coupled Beads Thumbnail
12016849

Pkg of 1, SARS-CoV-2 Beta S1 Viral Antigen Coupled Magnetic Beads for capturing neutralization antibodies that bind to Beta Spike 1 viral antigen, sufficient for 96 reactions. Requires Bio-Plex Pro Human SARS-CoV-2 Neutralization Antibody 2-Plex Panel for a complete assay or the use with biotinylated detection ACE2 receptor, Bio-Plex Pro Human Neutralization Reagent Kit, and standard to run a singleplex assay.

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Bio-Plex Pro SARS-CoV-2 Gamma RBD Coupled Beads Thumbnail
12016898

Pkg of 1, SARS-CoV-2 Gamma RBD Viral Antigen Coupled Magnetic Beads for capturing neutralization antibodies that bind to Gamma RBD viral antigen, sufficient for 96 reactions. Requires Bio-Plex Pro Human SARS-CoV-2 Neutralization Antibody 2-Plex Panel for a complete assay or the use with biotinylated detection ACE2 receptor, Bio-Plex Pro Neutralization Reagent Kit, and standard to run a singleplex assay.

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Bio-Plex Pro SARS-CoV-2 Delta RBD and Spike Trimer 2-Plex Coupled Beads
12017225

Pkg of 1, SARS-CoV-2 Delta RBD and Spike Trimer 2-Plex Viral Antigen Coupled Magnetic Beads for qualitatively capturing neutralization antibodies that bind to Delta RBD and Spike Trimer viral antigens, sufficient for 96 reactions. A ready-to-use positive control is included with the Delta 2-plex coupled beads. Requires Bio-Plex Pro Human SARS-CoV-2 Neutralization Antibody 2-Plex Panel for a complete assay or the use with biotinylated detection ACE2 receptor, and Bio-Plex Pro Neutralization Reagent Kit to run a 2-plex assay.

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Bio-Plex Pro SARS-CoV-2 Epsilon RBD Coupled Beads
12016875

Pkg of 1, SARS-CoV-2 Epsilon RBD Viral Antigen Coupled Magnetic Beads for capturing neutralization antibodies that bind to Epsilon RBD viral antigen, sufficient for 96 reactions. Requires Bio-Plex Pro Human SARS-CoV-2 Neutralization Antibody 2-Plex Panel for a complete assay or the use with biotinylated detection ACE2 receptor, Bio-Plex Pro Neutralization Reagent Kit, and standard to run a singleplex assay.

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Bio-Plex Pro SARS-CoV-2 Kappa RBD Coupled Beads Thumbnail
12016850

Pkg of 1, SARS-CoV-2 Kappa RBD Viral Antigen Coupled Magnetic Beads for capturing neutralization antibodies that bind to Kappa RBD viral antigen, sufficient for 96 reactions. Requires Bio-Plex Pro Human SARS-CoV-2 Neutralization Antibody 2-Plex Panel for a complete assay or the use with biotinylated detection ACE2 receptor, Bio-Plex Pro Neutralization Reagent Kit, and standard to run a singleplex assay.

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12014772-th.jpg
12015406

Pkg of 1, SARS-CoV-2 RBD viral protein coupled magnetic beads, for capturing anti-SARS-CoV-2 antibodies specific for RBD from multispecies serum or plasma, sufficient for 96 reactions; Requires isotype-specific detection antibody, reagent kit, and isotype-specific control set.

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12014771-th.jpg
12014771

Pkg of 1, SARS-CoV-2 S1 viral protein coupled magnetic beads, for capturing anti-SARS-CoV-2 antibodies specific for Spike 1 from multispecies serum or plasma, sufficient for 96 reactions; Requires isotype-specific detection antibody, reagent kit, and isotype-specific control set.

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Bio-Plex Pro SARS-CoV-2 D614G S1 Coupled Beads Thumbnail
12016838

Pkg of 1, SARS-CoV-2 D614G S1 Viral Antigen Coupled Magnetic Beads for capturing neutralization antibodies that bind to D614G Spike 1 viral antigen, sufficient for 96 reactions. Requires Bio-Plex Pro Human SARS-CoV-2 Neutralization Antibody 2-Plex Panel for a complete assay or the use with biotinylated detection ACE2 receptor, Bio-Plex Pro Neutralization Reagent Kit, and standard to run a singleplex assay.

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Bio-Plex Pro SARS-CoV-2 E484K RBD Coupled Beads Thumbnail
12016943

Pkg of 1, SARS-CoV-2 E484K RBD Viral Antigen Coupled Magnetic Beads for capturing neutralization antibodies that bind to E484K RBD viral antigen, sufficient for 96 reactions. Requires Bio-Plex Pro Human SARS-CoV-2 Neutralization Antibody 2-Plex Panel for a complete assay or the use with biotinylated detection ACE2 receptor, Bio-Plex Pro Neutralization Reagent Kit, and standard to run a singleplex assay.

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Bio-Plex Pro SARS-CoV-2 K417N RBD Coupled Beads Thumbnail
12016942

Pkg of 1, SARS-CoV-2 K417N RBD Viral Antigen Coupled Magnetic Beads for capturing neutralization antibodies that bind to K417N RBD viral antigen, sufficient for 96 reactions. Requires Bio-Plex Pro Human SARS-CoV-2 Neutralization Antibody 2-Plex Panel for a complete assay or the use with biotinylated detection ACE2 receptor, Bio-Plex Pro Human Neutralization Reagent Kit, and standard to run a singleplex assay.

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Bio-Plex Pro SARS-CoV-2 N501Y RBD Coupled Beads Thumbnail
12016869

Pkg of 1, SARS-CoV-2 N501Y RBD Viral Antigen Coupled Magnetic Beads for capturing neutralization antibodies that bind to N501Y RBD viral antigen, sufficient for 96 reactions. Requires Bio-Plex Pro Human SARS-CoV-2 Neutralization Antibody 2-Plex Panel for a complete assay or the use with biotinylated detection ACE2 receptor, Bio-Plex Pro Neutralization Reagent Kit, and standard to run a singleplex assay.

Documents

Documents

Number Description Options
10000147006
Instruction Manual, Bio-Plex Pro Human SARS-CoV-2 Neutralization Antibody Assays
10000147009
Bio-Plex Pro Human SARS-CoV-2 Neutralization Antibody Assays Quick Guide
3263
Bio-Plex™ Pro Human SARS-CoV-2 Neutralization Antibody Assays Product Information Sheet
10000148774
Bio-Plex Amine Coupling Kit Instruction Manual
3286
Development of New SARS-CoV-2 Variant Neutralization Antibody Assays Using the Bio-Plex Pro Human SARS-CoV-2 Neutralization Antibody Custom Assay Developer Kit Application Note