The ready-to-use PureZOL RNA isolation reagent is a versatile and efficient means of isolating high yields of RNA from a variety of sources, including cultured cell, animal and plant tissue, yeast, virus, and bacteria samples. The protocol is an improvement over the rapid, widely used, and proven method of RNA isolation developed by Chomczynski and Sacchi (1987). PureZOL RNA isolation reagent is a potent monophasic combination of phenol and the chaotropic agent guanidine isothiocyanate, which effectively lyses cells and tissues, deproteinates RNA, and inactivates endogenous nucleases in a single step. The single-solution format permits recovery of RNA from small quantities of tissues or cells, making it ideally suited for gene expression studies or whenever limited sample quantities are available. Protocols are scalable to accommodate a wide range of sample sizes. DNA and protein that are efficiently removed from the RNA, following phase separation, can be subsequently extracted from the same sample origin.
- Effective total RNA isolation from a wide variety of samples in less than 60 minutes
- Scalable starting sample amount
- Convenient isolation of RNA, DNA, and protein from the same sample source
Chomczynski P and Sacchi N (1987). Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem 162, 156-159.