PureZOL™ RNA Isolation Reagent

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Overview

RNA Isolation Selection Guide
  Aurum Total RNA Kits PureZOL RNA Isolation Reagent
Mini Fatty and Fibrous Tissue 96
Format Mini column Filtration (vacuum or spin) Mini column Filtration (vacuum or spin) 96-well plate Filtration (vacuum or spin)
Maximum starting amounts        
   Cultured cells 2 x 106 1 x 106 1 x 107
   Bacterial cells 2.4 x 109 8 x 108 2.4 x 109
   Yeast cells 3 x 107 2 x 107 3 x 107
   Animal tissue — hard 20 mg 100 mg 100 mg
   Animal tissue — soft    to moderately hard 40 mg 100 mg 100 mg
   Plant tissue 40 mg 100 mg 100 mg
Isolation method Silica membrane Lysis with PureZOL reagent, purification on silica membrane Silica membrane Organic extraction
Number of preps 10* or 50 mini preps 10* or 50 mini preps 2 x 96-well plate 50 or 100 preps (1 ml/prep)
Number of washes 3 3 3
DNase I included** Yes Yes Yes No
DNase I digest time 15 min (animal tissue, 25 min) 15 min 10 min
Total preparation time <50min (1.5 hr with DNase I) <50min (1.5 hr with DNase I) <60 min <60 min
Binding capacity >100 µg >100 µg >40 µg
Elution volume 2 x 40 µl 2 x 40 µl 80 µl 30–100 µl
Yield*** 28 µg/2 x 106 NIH 3T3 cells 96 µg/100 mg brain 10 µg/1 x 106 NIH 3T3 cells 1.15 µg/mg brain
  38 µg/2 x 106 HeLa cells 58 µg/100 mg breast 17 µg/1 x 106 HeLa cells 1.37 µg/mg fat
  20 µg/40 mg brain 85 µg/100 mg heart 11 µg/2 x 107 S. cerevisiae cells 1.2 µg/mg heart
  52 µg/20 mg thymus 63 µg/100 mg skin 5 µg/8 x 108 E. coli cells 0.66 µg/mg cartilage
  132 µg/40 mg spleen 54 µg/100 mg cartilage 5 µg/8 x 108 B. cereus cells 1.2 µg/mg skin
  36 µg/40 mg lung     1.0 µg/mg potato
  32 µg/40 mg potato     1.37 µg/1 x 105 293H cells
  28 µg/3 x 107 S. cerevisiae cells     0.1 µg/mg Arabidopsis
  54 µg/2.4 x 109 E. coli cells     0.38 µg/mg A. niger
        0.63 µg/2.4 x 107 E. coli cells
        0.31 µg/3 x 105 S. cerevisiae cells
Purity A260/A280 of 1.9–2.1 A260/A280 of 1.9–2.1 A260/A280 of 1.9–2.1 A260/A280 of 1.9–2.1
 

* Trial sizes of the Aurum total RNA mini kit and Aurum total RNA fatty and fibrous tissue kit are available.

** Removal not required.

*** Yields will vary depending on the development stage, tissue type or cell line, and growth conditions used.

PureZOL Performance

RNA isolated from various biological samples using the PureZOL RNA isloation reagent. Duplicate RNA samples (4 µg) from each sample type were denatured with glyoxal, resolved on a 1% TBE gel, and stained with ethidium bromide.

The ready-to-use PureZOL RNA isolation reagent is a versatile and efficient means of isolating high yields of RNA from a variety of sources, including cultured cell, animal and plant tissue, yeast, virus, and bacteria samples. The protocol is an improvement over the rapid, widely used, and proven method of RNA isolation developed by Chomczynski and Sacchi (1987).

PureZOL RNA isolation reagent is a potent monophasic combination of phenol and the chaotropic agent guanidine isothiocyanate, which effectively lyses cells and tissues, deproteinates RNA, and inactivates endogenous nucleases in a single step. The single-solution format permits recovery of RNA from small quantities of tissues or cells, making it ideally suited for gene expression studies or whenever limited sample quantities are available.

Protocols are scalable to accommodate a wide range of sample sizes. DNA and protein that are efficiently removed from the RNA, following phase separation, can be subsequently extracted from the same sample origin.

Features include:

  • Effective total RNA isolation from a wide variety of samples in less than 60 minutes
  • Scalable starting sample amount
  • Convenient isolation of RNA, DNA, and protein from the same sample source

Reference

Chomczynski P and Sacchi N (1987). Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem 162, 156-159.

Format Single-solution
Method Organic extraction
Number of preps 50 or 100 (1ml/prep)
Maximum starting material amounts 100 mg animal or plant tissues
1 x 107 cultured cells
2.4 x 109 bacterial cells
3 x 107 yeast cells
Purity A260/A280 of 1.9–2.1
Preparation time <60 min
PureZOL™ RNA Isolation Reagent

732-6880
50 ml, reagent for DNA- and protein-free RNA isolation

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PureZOL™ RNA Isolation Reagent

732-6890
100 ml, reagent for DNA- and protein-free RNA isolation

List Price:   $179.00
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Number Description Options
6090 Amplification Reagents and Plastics Brochure, Rev D Click to download [ Add to Cart (Free) ]
10001481 Instruction Manual, PureZOL RNA Isolation Reagent, Rev A Click to download