The Aurum total RNA 96 kit isolates high-quality DNA-free total RNA from cultured cells, bacteria, and yeast in a 96-well format for high-throughput total RNA isolation. The kit utilizes a stringent reagent composed of guanidine isothiocyanate and β-mercaptoethanol for efficient sample lysis and quick RNase inactivation, followed by purification on silica membrane in a 96-well plate format using a spin- or vacuum-mediated protocol. The entire purification process, including DNase I digest, is completed in less than 60 minutes. Isolated RNA is of the highest integrity and is suitable for the most discriminating applications.
- High-throughput total RNA isolation in less than 60 minutes
- RNase-free reagents and plasticware, including DNase I for removal of genomic DNA at no additional cost
- Rapid silica binding-based purification technology in a 96-well plate format
- Easy-to-use spin- or vacuum-compatible protocol
8 x 108 bacterial cells
2 x 107 yeast cells
10 µg/1 x 106 NIH 3T3 cells
17 µg/1 x 109 HeLa cells
11 µg/2 x 107 S. cerevisiae cells
5 µg/8 x 108 B. cereus cells
isolating total RNA from small amounts of
starting material (<500,000 cells or 10 mg of tissue).
developmental stage, tissue type or cell line,
and growth conditions used.
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