Description
Description
The Aurum total RNA 96 kit isolates high-quality DNA-free total RNA from cultured cells, bacteria, and yeast in a 96-well format for high-throughput total RNA isolation. The kit utilizes a stringent reagent composed of guanidine isothiocyanate and β-mercaptoethanol for efficient sample lysis and quick RNase inactivation, followed by purification on silica membrane in a 96-well plate format using a spin- or vacuum-mediated protocol. The entire purification process, including DNase I digest, is completed in less than 60 minutes. Isolated RNA is of the highest integrity and is suitable for the most discriminating applications.
Features include:
- High-throughput total RNA isolation in less than 60 minutes
- RNase-free reagents and plasticware, including DNase I for removal of genomic DNA at no additional cost
- Rapid silica binding-based purification technology in a 96-well plate format
- Easy-to-use spin- or vacuum-compatible protocol
Specifications
Specifications
Format
96-well plate
Method
Spin- or vacuum-mediated silica binding
Number of preps
192 (2 x 96-well plate)
Maximum starting material amounts
1 x 106 cultured cells
8 x 108 bacterial cells
2 x 107 yeast cells
8 x 108 bacterial cells
2 x 107 yeast cells
Binding capacity
>40 µg purified total RNA
Purity
A260/A280 of 1.9–2.1
RNA size distribution
>200 nucleotides
Preparation time
<60 min (including DNase I digest)
Maximum elution volume
80 µl
Minimum elution volume
30 µl*
10 µg/1 x 106 NIH 3T3 cells
17 µg/1 x 109 HeLa cells
11 µg/2 x 107 S. cerevisiae cells
10 µg/1 x 106 NIH 3T3 cells
17 µg/1 x 109 HeLa cells
11 µg/2 x 107 S. cerevisiae cells
Yield**
5 µg/8 x 108 E. Coli cells
5 µg/8 x 108 B. cereus cells
5 µg/8 x 108 B. cereus cells
*The elution volume is reduced to 30µl when
isolating total RNA from small amounts of
starting material (<500,000 cells or 10 mg of tissue).
isolating total RNA from small amounts of
starting material (<500,000 cells or 10 mg of tissue).
**Yields will vary depending on the
developmental stage, tissue type or cell line,
and growth conditions used.
developmental stage, tissue type or cell line,
and growth conditions used.
Ordering
Ordering
items
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Image
(Discontinued) Aurum™ Total RNA 96 Kit
7326800
DNA-free RNA isolation kit, includes RNA binding plates, collection microplates, lyophilized DNase I, RNase-free reagents, sealing tape, for 2 x 96-well preps
Accessories
Accessories
Image
(Discontinued) DNase I
7326828
Pkg of 1 vial, refill reagent for Aurum total RNA kits, RNase-free, lyophilized
Image
(Discontinued) Aurum DNase Dilution Solution
7326805
20 ml, refill reagent for Aurum total RNA kits
Image
(Discontinued) Aurum Total RNA Wash Low-Stringency Solution
7326804
60 ml, refill reagent for Aurum total RNA kits
Image
(Discontinued) Aurum Total RNA Wash High-Stringency Solution
7326803
150 ml, refill reagent for Aurum total RNA kits, RNase-free
Image
(Discontinued) Aurum Total RNA Lysis Solution
7326802
85 ml, refill reagent for Aurum total RNA kits, RNase-free
Image
(Discontinued) Aurum Total RNA Elution Solution
7326801
20 ml, refill reagent for Aurum total RNA kits, RNase-free
Documents
Documents
Login Required Number Description Options
6090
Amplification Reagents and Plastics Brochure
2925
Aurum Total RNA 96 Kit Protocol Overview
4110131
Instruction Manual, Aurum™ Total RNA 96 Kit, Rev C
7331
Protocol for Viral RNA Extraction using Aurum Total RNA Kits, Ver A