Aurum™ Total RNA Fatty and Fibrous Tissue Kit

Image
lsr_aurum_rna_fatty_fibrous_kit.jpg

Overview

The Aurum total RNA fatty and fibrous tissue kit produces high yields of exceptionally pure total RNA from samples that are difficult to disrupt.
Read full description Ordering Information

Description

Description

The Aurum total RNA fatty and fibrous tissue kit produces high yields of exceptionally pure total RNA from samples that are difficult to disrupt. The kit is perfect for purifications involving fatty and fibrous tissues or samples such as fungi that are rich in RNases. It also works well with most animal and plant tissues, cultured cells, yeast, and bacteria.

The Aurum total RNA fatty and fibrous tissue kit combines the best features of PureZOL™ RNA reagent, a potent phenol-based reagent that effectively lyses tissues and cells, with the speed of silica membrane technology in a spin-column format to yield high-quality, intact RNA in minimal time.

The entire purification process, including DNase I digestion, is completed in less than 60 minutes. The kit includes mini spin columns that can be processed using a choice of vacuum- or spin-mediated protocols. Isolated RNA is of the highest integrity and is suitable for the most discriminating applications.

Features include:

  • High-quality RNA isolation from difficult-to-process samples in less than 60 minutes
  • RNase-free reagents and plasticware; includes DNase I for the removal of genomic DNA
  • Rapid silica binding-based purification technology in a spin-column format
  • Easy-to-use spin- or vacuum-compatible protocol

Specifications

Specifications

Format
Mini column
Method
Spin- or vacuum-mediated silica binding
Number of preps
50
Maximum starting material amounts
100 mg animal or plant tissues
 
50 mg filamentous fungi
 
1 x 107 cultured cells
 
2.4 x 109 bacterial cells
 
3 x 107 yeast cells
Binding capacity
>100 µg purified total RNA
Purity
A260/A280 of 1.9–2.1
RNA size distribution
>200 nucleotides
Preparation time
30–60 min (including DNase I digest)
Maximum elution volume
80 µl (2 elutions of 40 µl each)
Minimum elution volume
30 µl*
Yield**
96 µg/100 mg brain
 
58 µg/100 mg breast
 
85 µg/100 mg heart
 
63 µg/100 mg skin
 
54 µg/100 mg cartilage
 
91 µg/100 mg plant tissue
 
12 µg/50 mg filamentous fungi
 
145 µg/1 x 107 293-H cultured cells
 
30 µg/2.4 x 109 E. coli
 
64 µg/3 x 107 S. cerevisiae
*The elution volume is reduced to 30µl
when isolating total RNA from small amounts of
starting material (<500,000 cells or 10 mg of tissue).
 
**Yields will vary depending on the development stage, tissue type or cell line, and growth conditions used.
 

Ordering

Ordering

items

Use the filters below to refine results!

Image
732-6830_tn.jpg
7326830
DNA-free RNA isolation kit, includes PureZOL RNA isolation reagent, RNA binding columns, all collection and sample tubes, lyophilized DNase I, RNase-free reagents and plasticware, for 50 preps
Image
732-6870_tn.jpg
7326870
DNA-free RNA isolation kit, includes columns and tubes, DNase I, RNase-free reagents (PureZOL RNA isolation reagent sold separately), for 50 preps

Accessories

Accessories

Documents

Documents

Number Description Options
6090
Amplification Reagents and Plastics Brochure
[ Add to Cart (Free) ]
5282
Aurum Total RNA Fatty and Fibrous Tissue Kit Flier, Rev A
6763
Gene Expression Real-Time PCR Workflow Flier
[ Add to Cart (Free) ]
5257
Aurum Total RNA Fatty and Fibrous Tissue Kit Protocol Overview, Rev A
10001298
Instruction Manual, Aurum Total RNA Fatty and Fibrous Tissue Kit, Rev B
5990
Gene Expression Analysis Solutions Brochure
[ Add to Cart (Free) ]