Aurum™ Total RNA Fatty and Fibrous Tissue Kit

Overview

The Aurum total RNA fatty and fibrous tissue kit produces high yields of exceptionally pure total RNA from samples that are difficult to disrupt.

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Description

The Aurum total RNA fatty and fibrous tissue kit produces high yields of exceptionally pure total RNA from samples that are difficult to disrupt. The kit is perfect for purifications involving fatty and fibrous tissues or samples such as fungi that are rich in RNases. It also works well with most animal and plant tissues, cultured cells, yeast, and bacteria.

The Aurum total RNA fatty and fibrous tissue kit combines the best features of PureZOL™ RNA reagent, a potent phenol-based reagent that effectively lyses tissues and cells, with the speed of silica membrane technology in a spin-column format to yield high-quality, intact RNA in minimal time.

The entire purification process, including DNase I digestion, is completed in less than 60 minutes. The kit includes mini spin columns that can be processed using a choice of vacuum- or spin-mediated protocols. Isolated RNA is of the highest integrity and is suitable for the most discriminating applications.

Features include:

High-quality RNA isolation from difficult-to-process samples in less than 60 minutes
RNase-free reagents and plasticware; includes DNase I for the removal of genomic DNA
Rapid silica binding-based purification technology in a spin-column format
Easy-to-use spin- or vacuum-compatible protocol

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Specifications

Format

Mini column

Method

Spin- or vacuum-mediated silica binding

Number of preps

Maximum starting material amounts

100 mg animal or plant tissues

50 mg filamentous fungi

1 x 10⁷ cultured cells

2.4 x 10⁹ bacterial cells

3 x 10⁷ yeast cells

Binding capacity

>100 µg purified total RNA

Purity

A₂₆₀/A₂₈₀ of 1.9–2.1

RNA size distribution

>200 nucleotides

Preparation time

30–60 min (including DNase I digest)

Maximum elution volume

80 µl (2 elutions of 40 µl each)

Minimum elution volume

30 µl*

Yield**

96 µg/100 mg brain

58 µg/100 mg breast

85 µg/100 mg heart

63 µg/100 mg skin

54 µg/100 mg cartilage

91 µg/100 mg plant tissue

12 µg/50 mg filamentous fungi

145 µg/1 x 10⁷ 293-H cultured cells

30 µg/2.4 x 10⁹ E. coli

64 µg/3 x 10⁷ S. cerevisiae

*The elution volume is reduced to 30µl
when isolating total RNA from small amounts of
starting material (<500,000 cells or 10 mg of tissue).

**Yields will vary depending on the development stage, tissue type or cell line, and growth conditions used.

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(Discontinued) Aurum™ Total RNA Fatty and Fibrous Tissue Kit

7326830

DNA-free RNA isolation kit, includes PureZOL RNA isolation reagent, RNA binding columns, all collection and sample tubes, lyophilized DNase I, RNase-free reagents and plasticware, for 50 preps