GMO Investigator Real-Time PCR Starter Kit

GMO Investigator Real-Time PCR Starter Kit

Teach the principles and workflow of real-time quantitative PCR (qPCR), from DNA extraction from food to analysis and quantification of genetically modified ingredients.

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Overview

Real-Time PCR (qPCR) Learning Center

RT-qPCR University

Courses designed to make you a qPCR expert.

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How much of your favorite foods are genetically modified?

Use the GMO Investigator Real-Time PCR Starter Kit to teach students the principles and techniques of real-time quantitative polymerase chain reaction (qPCR) within the context of testing food for the presence of genetic modifications. Students extract DNA from food items, then use real-time quantitative polymerase chain reaction (qPCR) to test for the presence of DNA sequences that are present in >85% of genetically modified (GM) crops: the 35S promoter of the cauliflower mosaic virus and the terminator of the nopaline synthase gene of Agrobacterium tumefaciens. Quantitate and determine what fraction of the food product is made with genetically modified ingredients, in the same manner as standard testing laboratories do!

The GMO Investigator Real-Time PCR Starter Kit combines the components of the GMO Investigator Kit with the reagents and plastics needed to perform the same activity using qPCR. Students analyze their favorite foods as they:

  • Learn the basics of qPCR analysis as they determine the presence or absence of GMO sequences in their choice of test food
  • Conduct sophisticated scientific procedures with multiple levels of controls to assess the validity of the results
  • Investigate how to perform melt-curve analysis and quantitation through use of a standard curve, all within the context of food testing and labeling

Use of this kit requires the use of real-time PCR system, such as the CFX Duet Real-Time PCR System for the Classroom.

Activities Include

  • Use real-time PCR to analyze a set of dilutions of positive control DNA (compare primer sets, use a standard curve for quantitation, perform a melt-curve analysis)
  • Extract DNA from food samples
  • Perform real-time PCR of DNA from food samples
  • Post-lab discussion and debate activity
  • Optional: compare results with those obtained by traditional PCR (the GMO Investigator Kit is included as a component of this kit)

Features and Benefits

  • Three-session laboratory activity, 45 min per session
  • Learn the molecular basis of DNA amplification reactions using real-time qPCR detection systems, all within a real-world application
  • Explore the impacts of pipetting accuracy and reliability on qPCR results

Related Products

Specifications

Kit contains sufficient materials for 8 student workstations (2–4 students per workstation), plus additional real-time reagents for further studies
 
GMO Investigator kit
1
SsoAdvanced Universal SYBR Green Supermix, 2x1 ml
1
Agarose powder, 25 g
1
Electrophoresis buffer, 50x TAE, 100 ml
1
Fast Blast™ DNA stain, 500x, 100 ml
1
Sterile water, 500 ml
1
PCR tube strips
120
Optical flat caps
120
Curriculum, including Teacher's Guide and Student Manual
1

Ordering

items

Use the filters below to refine results!

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(Discontinued) GMO Real-Time PCR Starter Kit
1662560EDU

Introductory real-time PCR classroom study kit, contains GMO Investigator Kit (1662500EDU) and additional real-time reagents; education use only

Refills

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(Discontinued) GMO Investigator Kit
1662500EDU
Gel electrophoresis classroom study kit, includes positive control, InstaGene matrix, master mix, primers, dye, test tubes/holders, curriculum, and more, for 32 students; education use only
166-2501EDU_tn.jpg
(Discontinued) GMO Investigator Kit Reagent Refill Pack
1662501EDU

Consumables refill kit for use with GMO Investigator Kit (1662500EDU), contains PCR master mix, primers, positive control, PCR molecular weight ruler, Orange G loading dye; education use only

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(Discontinued) Master Mix For PCR
1665009EDU
90 units, 1.2 ml includes 0.075 units per µl Taq DNA polymerase, reaction buffer, 4 mM MgCl2, 0.4 mM of each dNTP (dATP, dCTP, dGTP, dTTP); education use only
ssoadvanced-universal-sybr-green-supermix-172-5270-thumb.jpg
(Discontinued) SsoAdvanced Universal SYBR Green Supermix, 200 x 20 µl rxns, 2 ml (2 x 1 ml)
1725270EDU
2 ml (2 x 1 ml vials), 2x qPCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, SYBR Green I, ROX normalization dyes, for 200 x 20 µl reactions; for educational use only
TLS-0851EDU_tn.jpg
(Discontinued) Low-Profile 0.2 ml 8-Tube Strips without Caps
TLS0851EDU
Pkg of 120, thin-wall polypropylene 8-tube strips for PCR and low volume reactions, white, (960 tubes); education use only
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(Discontinued) Multiplate Low Profile 48-Well Unskirted PCR Plates
MLL4851EDU
Pkg of 50, white PCR plate, 8 x 6 format, one-component polypropylene, can be cut for fewer samples; education use only

Documents

Number Description Options
10002155
Instruction Manual, GMO Investigator Kit, Biotechnology Explorer, Rev C
M1662505
GMO Investigator Kit: A Quantitative Real-Time PCR Extension, Biotechnology Explorer, Application Note
3332
Solutions for Teaching Real-Time PCR
5396
Fast Gel Protocol — Easily Run Your DNA Gels in Under 10 Minutes — Bio-Rad Explorer, Ver D