SsoAdvanced Universal SYBR Green Supermix, 200 x 20 µl rxns, 2 ml (2 x 1 ml) #1725270EDU

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Overview

2 ml (2 x 1 ml vials), 2x qPCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, SYBR Green I, ROX normalization dyes, for 200 x 20 µl reactions; for educational use only

Description

Description

SsoAdvanced Universal SYBR Green Supermix is a high-performance real-time PCR supermix based on Bio-Rad's patented* Sso7d fusion protein technology. This supermix is formulated for a wide range of real-time PCR applications and for use with all ROX-dependent or -independent real-time PCR systems. The dsDNA binding protein, Sso7d, stabilizes the polymerase-template complex, providing superior inhibitor tolerance, increased processivity, specificity, and greater speed without affecting PCR sensitivity, efficiency, or reproducibility.

* U.S. patents 6,627,424; 7,541,170; and 7,560,260.

SsoAdvanced Universal SYBR Green Supermix Lets You:

  • Use any real-time PCR system — the universal reference dye in this supermix enables ROX normalization of qPCR data regardless of the ROX level requirements of the qPCR system
  • Achieve superior real-time PCR results under various conditions — our robust formulation delivers consistent performance in fast cycling across a broad range of reaction conditions, primer concentrations, and temperature ranges
  • Increase your qPCR sensitivity and efficiency of detection from compromised samples — the Sso7d fusion polymerase has increased resistance to a wide variety of PCR inhibitors, which helps provide better sensitivity and overall performance
  • Decrease time to results without compromising qPCR data quality — the Sso7d fusion polymerase and optimized buffer together provide rapid polymerization kinetics and instant polymerase activation
  • Obtain better results with Bio-Rad's PrimePCR assays — real-time PCR assays are expertly designed and wet-lab validated to ensure optimal assay performance

Instrument Compatibility

SsoAdvanced Universal SYBR Green Supermix is compatible with Bio-Rad Real-Time PCR Detection Systems and commercially available systems listed below:

Systems

Systems
Applied Biosystems
  • StepOne/StepOnePlus
  • 7500, ViiA 7
  • 7000, 7300, 7700, 7900HT, QuantStudio Systems
Stratagene
  • Mx3000P, 3005P, 4000
Eppendorf
  • Mastercycler ep realplex 2, 4
QIAGEN
  • Corbett Rotor-Gene 3000, 6000, Q
Roche
  • LightCycler 480, 96
  • LightCycler 1.0, 1.5, 2.0*
BioFire
  • LightScanner HR-1
  • LightScanner 32*

* BSA must be added according to instrument specifications

Applications and Uses of SsoAdvanced Universal SYBR Green Supermix

  • qPCR/real-time PCR
  • Gene expression analysis
  • Pathway analysis
  • Absolute quantification
  • Chromatin immunoprecipitation (ChIP) qPCR
  • Mutation detection
  • Pathogen detection
  • Viral detection (load)
  • Characterization of genetically modified organisms (GMO)
  • Genetic profiling

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