Macro-Prep® 25 S Resin

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Macro-Prep® 25 S Resin

Overview

Pressure versus Linear Velocity

Pressure vs. linear velocity for 1 M NaCl on Macro-Prep 25 S in a 1.6 cm (ID) x 12.5 cm (L) column.

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Macro-Prep 25 S Resin is a strong cation exchange resin that offers high-resolution separations at high flow rates with medium pressures. It possesses the same rigid, macroporous, and hydrophilic properties of 50 µm Macro-Prep High S support but in a 25 µm bead that offers higher resolution.

Features and Benefits of Macro-Prep 25 S Resin

  • Benchtop to pilot-scale separations, where maximum sample recovery and purity are important
  • Fewer chromatographic steps required
  • Removal of protein variants and other difficult contaminants that would require additional validation if present in the final formulation
More Information

Instruction Manual, Macro-Prep® 25 Resins (Bulletin 4006129)
Type of ion exchanger Strong cation
Functional group –SO3-
Total ionic capacity 110±30 µeq/ml
Median particle size 25 µm
Nominal pore size 725 Å
Shipping counterion NA+
Dynamic binding capacity >40 mg/ml lgG
Recommended linear flow rate range 50–300 cm/hr
Maximum linear flow rate 3,000 cm/hr
Chemical stability  
  1.0 M HCI >72 hr
  100% ethanol Excellent
  Chaotropic agents (8 M urea, 6 M guanidine-HCI) Excellent
pH stability 1–12
Volume changes  
  ph 4–10 <1%
  0.1–1.0 M Na Cl <4%
Autoclavability (121°C, 30 min) Yes
Storage solution 20% ethanol
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Macro-Prep 25 S Support

1530031
50 ml, cation exchange chromatography media, 25 µm particle size, for high-flow medium-pressure separations

0 1530031 /templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Media/153-0031_0921120701649.xml 1530031 09/21/09 12:07 PM N en 09/21/19 12:07 PM true true internet/product_SKU AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA LSR 2017-11-14 07:08:01 1530031-macro-prep-25-s-support 1530031 Macro-Prep 25 S Support Macro-Prep 25 S Support 50 ml, cation exchange chromatography media, 25 µm particle size, for high-flow medium-pressure separations 50 ml, cation exchange chromatography media, 25 µm particle size, for high-flow medium-pressure separations 50 ml, cation exchange chromatography media, 25 µm particle size, for high-flow medium-pressure separations /webroot/web/images/ps/products/ion_exchange_media/sku_thumb/global/153-0031_tn.jpg /webroot/web/images/ps/products/ion_exchange_media/sku_view/global/153-0031_view.jpg /webroot/web/images/ps/products/ion_exchange_media/sku_feature/global/153-0031_feature.jpg RepEx 153-0031 50 ml, cation exchange chromatography media, 25 µm particle size, for high-flow medium-pressure separations 09/21/09 12:07 PM 09/21/19 12:07 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Chromatography/Chromatography-Media N 0 1530031, macro-prep, 25, s, support, cation, exchange, chromatography, media, 25um, particle, size, high-flow, medium-pressure, separation, protein, purification, 50, macroprep false false false /prd/en/CA/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1530031&CartQuote=QT&quantity= false true false $950.00 false null /prd/en/CA/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1530031&AddToDefaultWishList=true&quantity= true false true true false true true true /prd/en/CA/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1530031&quantity= 1530031
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Macro-Prep 25 S Support

1530032
200 ml, cation exchange chromatography media, 25 µm particle size, for high-flow medium-pressure separations

1 1530032 /templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Media/153-0032_0921120701621.xml 1530032 09/21/09 12:07 PM N en 09/21/19 12:07 PM true true internet/product_SKU AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA LSR 2017-11-14 07:08:01 1530032-macro-prep-25-s-support 1530032 Macro-Prep 25 S Support Macro-Prep 25 S Support 200 ml, cation exchange chromatography media, 25 µm particle size, for high-flow medium-pressure separations 200 ml, cation exchange chromatography media, 25 µm particle size, for high-flow medium-pressure separations 200 ml, cation exchange chromatography media, 25 µm particle size, for high-flow medium-pressure separations /webroot/web/images/ps/products/ion_exchange_media/sku_thumb/global/153-0032_tn.jpg /webroot/web/images/ps/products/ion_exchange_media/sku_view/global/153-0032_view.jpg /webroot/web/images/ps/products/ion_exchange_media/sku_feature/global/153-0032_feature.jpg RepEx 153-0032 200 ml, cation exchange chromatography media, 25 µm particle size, for high-flow medium-pressure separations 09/21/09 12:07 PM 09/21/19 12:07 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Chromatography/Chromatography-Media N 0 1530032, macro-prep, 25, s, support, cation, exchange, chromatography, media, 25um, particle, size, high-flow, medium-pressure, separation, protein, purification, 200, macroprep false false false /prd/en/CA/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1530032&CartQuote=QT&quantity= false true false $3,335.00 false null /prd/en/CA/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1530032&AddToDefaultWishList=true&quantity= true false true true false true true true /prd/en/CA/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1530032&quantity= 1530032
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Macro-Prep 25 S Support

153-0033
1 L, strong cation exchange chromatography media, 25 μm particle size

2 1530033 /templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Media/153-0033_0921120701602.xml 1530033 09/21/09 12:07 PM N en 09/21/19 12:07 PM true true internet/product_SKU AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA LSR 2017-11-14 07:08:01 1530033-macro-prep-25-s-support 1530033 Macro-Prep 25 S Support Macro-Prep 25 S Support 1 L, strong cation exchange chromatography media, 25 μm particle size 1 L, strong cation exchange chromatography media, 25 μm particle size 1 L, strong cation exchange chromatography media, 25 μm particle size /webroot/web/images/lsr/products/chromatography/sku_thumb/global/153-0033_tn.jpg /webroot/web/images/lsr/products/chromatography/sku_view/global/153-0033_view.jpg /webroot/web/images/lsr/products/chromatography/sku_feature/global/153-0033_feature.jpg RepEx 153-0033 1 L, strong cation exchange chromatography media, 25 μm particle size 09/21/09 12:07 PM 09/21/19 12:07 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Chromatography/Chromatography-Media N 0 1530033, macro-prep, 25, s, support, cation, exchange, chromatography, media, 25um, particle, size, high-flow, medium-pressure, separation, protein, purification, liter, macroprep false false false /prd/en/CA/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1530033&CartQuote=QT&quantity= false true false null false null /prd/en/CA/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1530033&AddToDefaultWishList=true&quantity= false false true true false true false true /prd/en/CA/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1530033&quantity= 153-0033
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Macro-Prep 25 S Support

153-0034
5 L, strong cation exchange chromatography media, 25 μm particle size

3 1530034 /templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Media/153-0034_0921120701579.xml 1530034 09/21/09 12:07 PM N en 09/21/19 12:07 PM true true internet/product_SKU AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA LSR 2017-11-14 07:08:01 1530034-macro-prep-25-s-support 1530034 Macro-Prep 25 S Support Macro-Prep 25 S Support 5 L, strong cation exchange chromatography media, 25 μm particle size 5 L, strong cation exchange chromatography media, 25 μm particle size 5 L, strong cation exchange chromatography media, 25 μm particle size /webroot/web/images/ps/products/ion_exchange_media/sku_thumb/global/153-0034_tn.jpg /webroot/web/images/ps/products/ion_exchange_media/sku_view/global/153-0034_view.jpg /webroot/web/images/ps/products/ion_exchange_media/sku_feature/global/153-0034_feature.jpg RepEx 153-0034 5 L, strong cation exchange chromatography media, 25 μm particle size 09/21/09 12:07 PM 09/21/19 12:07 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Chromatography/Chromatography-Media N 0 1530034, macro-prep, 25, s, support, cation, exchange, chromatography, media, 25um, particle, size, high-flow, medium-pressure, separation, protein, purification, liter, macroprep false false false /prd/en/CA/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1530034&CartQuote=QT&quantity= false true false null false null /prd/en/CA/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1530034&AddToDefaultWishList=true&quantity= false false true true false true false true /prd/en/CA/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1530034&quantity= 153-0034
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Protein Standard for Cation Exchange Chromatography

1250562
Pkg of 6 vials, lyophilized mixture of equine myoglobin, Ribonuclease A, cytochrome c, for use in ion exchange chromatography

3 1250562 /templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Media/125-0562_0921120700271.xml 1250562 09/21/09 12:07 PM N en 09/21/19 12:07 PM true true internet/product_SKU AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA LSR 2017-11-14 07:07:58 1250562-protein-standard-for-cation-exchange-chromatography 1250562 Protein Standard for Cation Exchange Chromatography Protein Standard for Cation Exchange Chromatography Pkg of 6 vials, lyophilized mixture of equine myoglobin, Ribonuclease A, cytochrome c, for use in ion exchange chromatography Pkg of 6 vials, lyophilized mixture of equine myoglobin, Ribonuclease A, cytochrome c, for use in ion exchange chromatography Pkg of 6 vials, lyophilized mixture of equine myoglobin, Ribonuclease A, cytochrome c, for use in ion exchange chromatography /webroot/web/images/ps/products/chrom_media_sampling/sku_thumb/global/125-0562_tn.jpg /webroot/web/images/ps/products/chrom_media_sampling/sku_view/global/125-0562_view.jpg /webroot/web/images/ps/products/chrom_media_sampling/sku_feature/global/125-0562_feature.jpg RepEx 125-0562 Pkg of 6 vials, lyophilized mixture of equine myoglobin, Ribonuclease A, cytochrome c, for use in ion exchange chromatography 09/21/09 12:07 PM 09/21/19 12:07 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Chromatography/Chromatography-Media N 0 1250562, protein, standard, cation, exchange, chromatography, ion, marker, ladder, lyophilized, mixture, separation, purification false false false /prd/en/CA/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1250562&CartQuote=QT&quantity= false true false $401.00 false null /prd/en/CA/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1250562&AddToDefaultWishList=true&quantity= true false true true false true true true /prd/en/CA/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1250562&quantity= 1250562
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Number Description Options
2292 Macro-Prep Ion Exchange Supports Product Information Sheet, Rev A Click to download
4006129 Instruction Manual, Macro-Prep 25 Ion Exchange Supports, Rev B Click to download
5662 Process Chromatography Applications Laboratory Brochure, Rev B Click to download

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